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O88428

- PAPS2_MOUSE

UniProt

O88428 - PAPS2_MOUSE

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Protein

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2

Gene

Papss2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth.

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241Phosphoserine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 578ATPSequence Analysis

GO - Molecular functioni

  1. adenylylsulfate kinase activity Source: MGI
  2. ATP binding Source: UniProtKB-KW
  3. nucleotidyltransferase activity Source: UniProtKB
  4. sulfate adenylyltransferase (ATP) activity Source: UniProtKB

GO - Biological processi

  1. blood coagulation Source: MGI
  2. bone development Source: MGI
  3. sulfate assimilation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_214239. Transport and synthesis of PAPS.
UniPathwayiUPA00097.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Short name:
PAPS synthase 2
Short name:
PAPSS 2
Alternative name(s):
Sulfurylase kinase 2
Short name:
SK 2
Short name:
SK2
Including the following 2 domains:
Sulfate adenylyltransferase (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase
Sulfate adenylate transferase
Short name:
SAT
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate synthase
APS kinase
Adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenylylsulfate 3'-phosphotransferase
Gene namesi
Name:Papss2
Synonyms:Atpsk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1330223. Papss2.

Pathology & Biotechi

Involvement in diseasei

Defects in Papss2 are the cause of brachymorphism (bm), a autosomal recessive disease, which is characterized by abnormal hepatic detoxification, bleeding times and postnatal growth, such as dome-shaped skull, short thick tail, and shortened but not widened limbs. The abnormal postnatal growth has been attributed to undersulfation of cartilage proteoglycans.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2PRO_0000105962Add
BLAST

Proteomic databases

MaxQBiO88428.
PaxDbiO88428.
PRIDEiO88428.

PTM databases

PhosphoSiteiO88428.

Expressioni

Tissue specificityi

Expressed in liver, cartilage, skin and brain.

Gene expression databases

BgeeiO88428.
CleanExiMM_PAPSS2.
GenevestigatoriO88428.

Interactioni

Protein-protein interaction databases

IntActiO88428. 4 interactions.
MINTiMINT-1856155.

Structurei

3D structure databases

ProteinModelPortaliO88428.
SMRiO88428. Positions 22-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – ?211211Adenylyl-sulfate kinaseAdd
BLAST
Regioni?212 – 621410Sulfate adenylyltransferaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi517 – 5215PP-motif

Sequence similaritiesi

In the N-terminal section; belongs to the APS kinase family.Curated
In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0529.
GeneTreeiENSGT00390000009613.
HOGENOMiHOG000069045.
HOVERGENiHBG053503.
InParanoidiO88428.
KOiK13811.
OMAiHGIPCYS.
OrthoDBiEOG74TWZ4.
TreeFamiTF313143.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
[Graphical view]
PfamiPF01583. APS_kinase. 1 hit.
PF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.

Sequencei

Sequence statusi: Complete.

O88428-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSANFKMNHK RDQQKSTNVV YQAHHVSRNK RGQVVGTRGG FRGCTVWLTG
60 70 80 90 100
LSGAGKTTIS FALEEYLVSH AIPCYSLDGD NVRHGLNKNL GFSAGDREEN
110 120 130 140 150
IRRIAEVARL FADAGLVCIT SFISPFAKDR ENARKIHESA GLPFFEIFVD
160 170 180 190 200
APLNICESRD VKGLYKRARA GEIKGFTGID SDYEKPETPE CVLKTNLSSV
210 220 230 240 250
SDCVQQVVEL LQEQNIVPHT TIKGIHELFV PENKVDQIRA EAETLPSLPI
260 270 280 290 300
TKLDLQWVQI LSEGWATPLK GFMREKEYLQ TLHFDTLLDG VVPRDGVINM
310 320 330 340 350
SIPIVLPVSA DDKARLEGCS KFALMYEGRR VALLQDPEFY EHRKEERCSR
360 370 380 390 400
VWGTATAKHP HIKMVMESGD WLVGGDLQVL ERIRWDDGLD QYRLTPLELK
410 420 430 440 450
QKCKDMNADA VFAFQLRNPV HNGHALLMQD TRRRLLERGY KHPVLLLHPL
460 470 480 490 500
GGWTKDDDVP LEWRMKQHAA VLEERVLDPK STIVAIFPSP MLYAGPTEVQ
510 520 530 540 550
WHCRCRMIAG ANFYIVGRDP AGMPHPETKK DLYEPTHGGK VLSMAPGLTS
560 570 580 590 600
VEIIPFRVAA YNKIKKAMDF YDPARHEEFD FISGTRMRKL AREGEDPPDG
610 620
FMAPKAWKVL TDYYRSLEKT N
Length:621
Mass (Da):70,351
Last modified:July 27, 2011 - v2
Checksum:i27FD5377A79EFD61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51F → S in AAC40191. (PubMed:9671738)Curated
Sequence conflicti290 – 2945Missing in AAC98687. (PubMed:9771708)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti79 – 791G → R in bm; activity abolished. 2 Publications
Natural varianti109 – 1091R → K.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052453 mRNA. Translation: AAC40191.1.
AF085144 mRNA. Translation: AAC98687.1.
BC090997 mRNA. Translation: AAH90997.1.
CCDSiCCDS37960.1.
RefSeqiNP_035994.2. NM_011864.3.
UniGeneiMm.203916.

Genome annotation databases

EnsembliENSMUST00000025833; ENSMUSP00000025833; ENSMUSG00000024899.
GeneIDi23972.
KEGGimmu:23972.
UCSCiuc008hfl.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052453 mRNA. Translation: AAC40191.1 .
AF085144 mRNA. Translation: AAC98687.1 .
BC090997 mRNA. Translation: AAH90997.1 .
CCDSi CCDS37960.1.
RefSeqi NP_035994.2. NM_011864.3.
UniGenei Mm.203916.

3D structure databases

ProteinModelPortali O88428.
SMRi O88428. Positions 22-619.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O88428. 4 interactions.
MINTi MINT-1856155.

PTM databases

PhosphoSitei O88428.

Proteomic databases

MaxQBi O88428.
PaxDbi O88428.
PRIDEi O88428.

Protocols and materials databases

DNASUi 23972.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025833 ; ENSMUSP00000025833 ; ENSMUSG00000024899 .
GeneIDi 23972.
KEGGi mmu:23972.
UCSCi uc008hfl.2. mouse.

Organism-specific databases

CTDi 9060.
MGIi MGI:1330223. Papss2.

Phylogenomic databases

eggNOGi COG0529.
GeneTreei ENSGT00390000009613.
HOGENOMi HOG000069045.
HOVERGENi HBG053503.
InParanoidi O88428.
KOi K13811.
OMAi HGIPCYS.
OrthoDBi EOG74TWZ4.
TreeFami TF313143.

Enzyme and pathway databases

UniPathwayi UPA00097 .
Reactomei REACT_214239. Transport and synthesis of PAPS.

Miscellaneous databases

ChiTaRSi Papss2. mouse.
NextBioi 303861.
PROi O88428.
SOURCEi Search...

Gene expression databases

Bgeei O88428.
CleanExi MM_PAPSS2.
Genevestigatori O88428.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00065. Adenylyl_sulf_kinase.
InterProi IPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
[Graphical view ]
Pfami PF01583. APS_kinase. 1 hit.
PF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A member of a new family of sulfate activating enzymes causes murine brachymorphism."
    Kurima K., Warman M.L., Krishnan S., Domowicz M., Krueger R.C. Jr., Deyrup A., Schwartz N.B.
    Proc. Natl. Acad. Sci. U.S.A. 95:8681-8685(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT BM ARG-79.
    Tissue: Liver.
  2. "Mutations in orthologous genes in human spondyloepimetaphyseal dysplasia and the brachymorphic mouse."
    ul Haque M.F., King L.M., Krakow D., Cantor R.M., Rusiniak M.E., Swank R.T., Superti-Furga A., Haque S., Abbas H., Ahmad W., Ahmad M., Cohn D.H.
    Nat. Genet. 20:157-162(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT BM ARG-79, VARIANT LYS-109.
    Strain: PWK.
    Tissue: Spleen.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiPAPS2_MOUSE
AccessioniPrimary (citable) accession number: O88428
Secondary accession number(s): Q5BKP4, Q9Z274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3