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Protein

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2

Gene

Papss2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth.

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: sulfate assimilation

This protein is involved in the pathway sulfate assimilation, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway sulfate assimilation and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92Adenylyl sulfateBy similarity1
Binding sitei162Adenylyl sulfateBy similarity1
Binding sitei198ATP 1; via carbonyl oxygenBy similarity1
Binding sitei559ATP 2; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 58ATP 1By similarity6
Nucleotide bindingi415 – 418ATP 2By similarity4
Nucleotide bindingi517 – 521ATP 2By similarity5

GO - Molecular functioni

  • adenylylsulfate kinase activity Source: MGI
  • ATP binding Source: UniProtKB-KW
  • nucleotidyltransferase activity Source: UniProtKB
  • sulfate adenylyltransferase (ATP) activity Source: MGI

GO - Biological processi

  • 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process Source: GO_Central
  • blood coagulation Source: MGI
  • bone development Source: MGI
  • sulfate assimilation Source: MGI

Keywordsi

Molecular functionKinase, Nucleotidyltransferase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-174362. Transport and synthesis of PAPS.
UniPathwayiUPA00097.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Short name:
PAPS synthase 2
Short name:
PAPSS 2
Alternative name(s):
Sulfurylase kinase 2
Short name:
SK 2
Short name:
SK2
Including the following 2 domains:
Sulfate adenylyltransferase (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase
Sulfate adenylate transferase
Short name:
SAT
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate synthase
APS kinase
Adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenylylsulfate 3'-phosphotransferase
Gene namesi
Name:Papss2
Synonyms:Atpsk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1330223. Papss2.

Pathology & Biotechi

Involvement in diseasei

Defects in Papss2 are the cause of brachymorphism (bm), a autosomal recessive disease, which is characterized by abnormal hepatic detoxification, bleeding times and postnatal growth, such as dome-shaped skull, short thick tail, and shortened but not widened limbs. The abnormal postnatal growth has been attributed to undersulfation of cartilage proteoglycans.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059621 – 621Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2Add BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO88428.
PaxDbiO88428.
PeptideAtlasiO88428.
PRIDEiO88428.

PTM databases

iPTMnetiO88428.
PhosphoSitePlusiO88428.

Expressioni

Tissue specificityi

Expressed in liver, cartilage, skin and brain.

Gene expression databases

BgeeiENSMUSG00000024899.
CleanExiMM_PAPSS2.
GenevisibleiO88428. MM.

Interactioni

Protein-protein interaction databases

IntActiO88428. 4 interactors.
MINTiMINT-1856155.
STRINGi10090.ENSMUSP00000025833.

Structurei

3D structure databases

ProteinModelPortaliO88428.
SMRiO88428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 216Adenylyl-sulfate kinaseCuratedAdd BLAST216
Regioni80 – 83Adenylyl sulfate bindingBy similarity4
Regioni97 – 100Adenylyl sulfate bindingBy similarity4
Regioni123 – 124Adenylyl sulfate bindingBy similarity2
Regioni175 – 176Adenylyl sulfate bindingBy similarity2
Regioni225 – 621Sulfate adenylyltransferaseCuratedAdd BLAST397

Sequence similaritiesi

In the N-terminal section; belongs to the APS kinase family.Curated
In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG0635. Eukaryota.
KOG4238. Eukaryota.
COG0529. LUCA.
COG2046. LUCA.
GeneTreeiENSGT00390000009613.
HOGENOMiHOG000069045.
HOVERGENiHBG053503.
InParanoidiO88428.
KOiK13811.
OMAiTCAKHPH.
OrthoDBiEOG091G07ZR.
TreeFamiTF313143.

Family and domain databases

CDDicd02027. APSK. 1 hit.
cd00517. ATPS. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiView protein in InterPro
IPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
PfamiView protein in Pfam
PF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.

Sequencei

Sequence statusi: Complete.

O88428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANFKMNHK RDQQKSTNVV YQAHHVSRNK RGQVVGTRGG FRGCTVWLTG
60 70 80 90 100
LSGAGKTTIS FALEEYLVSH AIPCYSLDGD NVRHGLNKNL GFSAGDREEN
110 120 130 140 150
IRRIAEVARL FADAGLVCIT SFISPFAKDR ENARKIHESA GLPFFEIFVD
160 170 180 190 200
APLNICESRD VKGLYKRARA GEIKGFTGID SDYEKPETPE CVLKTNLSSV
210 220 230 240 250
SDCVQQVVEL LQEQNIVPHT TIKGIHELFV PENKVDQIRA EAETLPSLPI
260 270 280 290 300
TKLDLQWVQI LSEGWATPLK GFMREKEYLQ TLHFDTLLDG VVPRDGVINM
310 320 330 340 350
SIPIVLPVSA DDKARLEGCS KFALMYEGRR VALLQDPEFY EHRKEERCSR
360 370 380 390 400
VWGTATAKHP HIKMVMESGD WLVGGDLQVL ERIRWDDGLD QYRLTPLELK
410 420 430 440 450
QKCKDMNADA VFAFQLRNPV HNGHALLMQD TRRRLLERGY KHPVLLLHPL
460 470 480 490 500
GGWTKDDDVP LEWRMKQHAA VLEERVLDPK STIVAIFPSP MLYAGPTEVQ
510 520 530 540 550
WHCRCRMIAG ANFYIVGRDP AGMPHPETKK DLYEPTHGGK VLSMAPGLTS
560 570 580 590 600
VEIIPFRVAA YNKIKKAMDF YDPARHEEFD FISGTRMRKL AREGEDPPDG
610 620
FMAPKAWKVL TDYYRSLEKT N
Length:621
Mass (Da):70,351
Last modified:July 27, 2011 - v2
Checksum:i27FD5377A79EFD61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5F → S in AAC40191 (PubMed:9671738).Curated1
Sequence conflicti290 – 294Missing in AAC98687 (PubMed:9771708).Curated5

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti79G → R in bm; activity abolished. 2 Publications1
Natural varianti109R → K1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052453 mRNA. Translation: AAC40191.1.
AF085144 mRNA. Translation: AAC98687.1.
BC090997 mRNA. Translation: AAH90997.1.
CCDSiCCDS37960.1.
RefSeqiNP_035994.2. NM_011864.3.
UniGeneiMm.203916.

Genome annotation databases

EnsembliENSMUST00000025833; ENSMUSP00000025833; ENSMUSG00000024899.
GeneIDi23972.
KEGGimmu:23972.
UCSCiuc008hfl.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPAPS2_MOUSE
AccessioniPrimary (citable) accession number: O88428
Secondary accession number(s): Q5BKP4, Q9Z274
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 5, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families