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Protein

Polypeptide N-acetylgalactosaminyltransferase 5

Gene

Galnt5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity toward Muc2, Muc1b, rMuc-2 or mG-Muc substrates.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei526SubstrateBy similarity1
Binding sitei555SubstrateBy similarity1
Metal bindingi578ManganeseBy similarity1
Binding sitei579SubstrateBy similarity1
Metal bindingi580ManganeseBy similarity1
Binding sitei685SubstrateBy similarity1
Metal bindingi713ManganeseBy similarity1
Binding sitei716SubstrateBy similarity1
Binding sitei721SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.41. 5301.
ReactomeiR-RNO-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 5 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 5
Short name:
GalNAc-T5
Short name:
pp-GaNTase 5
Protein-UDP acetylgalactosaminyltransferase 5
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5
Gene namesi
Name:Galnt5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi620361. Galnt5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini36 – 930LumenalSequence analysisAdd BLAST895

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591121 – 930Polypeptide N-acetylgalactosaminyltransferase 5Add BLAST930

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Glycosylationi198N-linked (GlcNAc...)Sequence analysis1
Glycosylationi213N-linked (GlcNAc...)Sequence analysis1
Modified residuei285PhosphoserineBy similarity1
Glycosylationi287N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Glycosylationi387N-linked (GlcNAc...)Sequence analysis1
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi476 ↔ 708PROSITE-ProRule annotation
Glycosylationi568N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi699 ↔ 779PROSITE-ProRule annotation
Glycosylationi766N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi812 ↔ 825PROSITE-ProRule annotation
Glycosylationi817N-linked (GlcNAc...)Sequence analysis1
Glycosylationi835N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi848 ↔ 863PROSITE-ProRule annotation
Disulfide bondi898 ↔ 913PROSITE-ProRule annotation
Glycosylationi902N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88422.
PRIDEiO88422.

Expressioni

Tissue specificityi

Predominantly expressed in sublingual gland. Expressed at lower level in stomach and small intestine. Weakly or not expressed in submandibular gland, parotid gland, kidney, liver, heart, brain, spleen, lung, skeletal muscle, testis, ovary, cervix and uterus.1 Publication

Gene expression databases

BgeeiENSRNOG00000004645.
GenevisibleiO88422. RN.

Interactioni

Subunit structurei

Interacts with EXT2. Does not interact with EXT1, EXTL1 or EXTL3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006319.

Structurei

3D structure databases

ProteinModelPortaliO88422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini794 – 925Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni485 – 594Catalytic subdomain AAdd BLAST110
Regioni654 – 716Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736. Eukaryota.
ENOG410XPMK. LUCA.
GeneTreeiENSGT00760000118828.
HOGENOMiHOG000231869.
HOVERGENiHBG051698.
InParanoidiO88422.
KOiK00710.
OMAiSHVVIIT.
OrthoDBiEOG091G085O.
PhylomeDBiO88422.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIRKFFRG SGRVLAFIFV ASVIWLLFDM AALRLSFSEI NTGILKEDIM
60 70 80 90 100
RREQTGFRVE ADQMTILSPS SRGMRPPRNG AGGKESFRKA ENRVLKVEEN
110 120 130 140 150
VDQVQRKGKM QFLLGRGKAV SLWHRTHVQT LPVTLPMQKT QGRDSKPEVS
160 170 180 190 200
SLHMMSKQTT VLGSEKDSFT VSRGVPLNKT AEHTETLDKK QEAPENYNLS
210 220 230 240 250
SDTSKQASQR ALNVTISVRT DRSKQQSQTV TKSSIQFASL PILKPEEVTV
260 270 280 290 300
TKKTEAQGKD LKYEAHKARP LLKFTADVGH LKKQSTNETG LGVLPEADGA
310 320 330 340 350
KVAPGKKLNF SESQIVIITK EEGQKTDTKE VPNSKIQTVF PKLLGESQGK
360 370 380 390 400
HIPRSQSQTL SSPLAPKRAV SQSKPTLAEE LHTARSNLTA KATTVGHQQS
410 420 430 440 450
HANISENPGK HHVLRIDVTL SPRDLNAPGQ FGRPVVVPPG KKKEAEQRWK
460 470 480 490 500
EGNFNVYLSD LIPVDRAIED TRPAGCAEQL VHNDLPTTSI IMCFVDEVWS
510 520 530 540 550
ALLRSVHSVL NRSPPHLIKE ILLVDDFSTK DYLKANLDKY MSQFPKVRIL
560 570 580 590 600
RLKERHGLIR ARLAGAQNAT GDVLTFLDSH VECNVGWLEP LLERVYLNRK
610 620 630 640 650
KVACPVIEVI NDKDMSYMTV DNFQRGVFTW PMNFGWRTIP PDVIAKNGIK
660 670 680 690 700
ETDIIRCPVM AGGLFSIDKS YFYELGTYDP GLDVWGGENM ELSFKVWMCG
710 720 730 740 750
GEIEIIPCSR VGHIFRNDNP YSFPKDRMKT VERNLVRVAE VWLDEYKELF
760 770 780 790 800
YGHGDHLIDQ GLDVGNLTQQ RELRKKLKCQ SFKWYLDNVF PDLKAPVVRA
810 820 830 840 850
SGVFINLALG KCVSIKNITV VLEDCDGSSE LQQFNYTWVR LIKHGEWCVA
860 870 880 890 900
PIPDKGSLTL YPCDNRNNRL KWLHRSASAF HPELVDHIVF ESYQQLLCME
910 920 930
GNFSQKTLKL AACNPTEPQQ KWKFEKYYDV
Length:930
Mass (Da):105,120
Last modified:November 1, 1998 - v1
Checksum:i1C906A76F320A225
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049344 mRNA. Translation: AAC69708.1.
RefSeqiNP_113984.1. NM_031796.1.
UniGeneiRn.30048.

Genome annotation databases

EnsembliENSRNOT00000006319; ENSRNOP00000006319; ENSRNOG00000004645.
GeneIDi83627.
KEGGirno:83627.
UCSCiRGD:620361. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049344 mRNA. Translation: AAC69708.1.
RefSeqiNP_113984.1. NM_031796.1.
UniGeneiRn.30048.

3D structure databases

ProteinModelPortaliO88422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006319.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Proteomic databases

PaxDbiO88422.
PRIDEiO88422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006319; ENSRNOP00000006319; ENSRNOG00000004645.
GeneIDi83627.
KEGGirno:83627.
UCSCiRGD:620361. rat.

Organism-specific databases

CTDi11227.
RGDi620361. Galnt5.

Phylogenomic databases

eggNOGiKOG3736. Eukaryota.
ENOG410XPMK. LUCA.
GeneTreeiENSGT00760000118828.
HOGENOMiHOG000231869.
HOVERGENiHBG051698.
InParanoidiO88422.
KOiK00710.
OMAiSHVVIIT.
OrthoDBiEOG091G085O.
PhylomeDBiO88422.
TreeFamiTF313267.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.41. 5301.
ReactomeiR-RNO-913709. O-linked glycosylation of mucins.

Miscellaneous databases

PROiO88422.

Gene expression databases

BgeeiENSRNOG00000004645.
GenevisibleiO88422. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGALT5_RAT
AccessioniPrimary (citable) accession number: O88422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.