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O88420

- SCN8A_RAT

UniProt

O88420 - SCN8A_RAT

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Protein

Sodium channel protein type 8 subunit alpha

Gene

Scn8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi891 – 8988ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. sodium ion binding Source: RGD
  3. voltage-gated sodium channel activity Source: RGD

GO - Biological processi

  1. membrane depolarization during action potential Source: RefGenome
  2. myelination Source: BHF-UCL
  3. neuronal action potential Source: RGD
  4. peripheral nervous system development Source: BHF-UCL
  5. sodium ion transmembrane transport Source: GOC
  6. sodium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium channel protein type 8 subunit alpha
Alternative name(s):
Peripheral nerve protein type 4
Short name:
PN4
Sodium channel 6
Short name:
NaCh6
Sodium channel protein type VIII subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.6
Gene namesi
Name:Scn8a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3638. Scn8a.

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 127127CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei128 – 15124Helical; Name=S1 of repeat ISequence AnalysisAdd
BLAST
Topological domaini152 – 1598ExtracellularSequence Analysis
Transmembranei160 – 17920Helical; Name=S2 of repeat ISequence AnalysisAdd
BLAST
Topological domaini180 – 19213CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei193 – 21119Helical; Name=S3 of repeat ISequence AnalysisAdd
BLAST
Topological domaini212 – 2176ExtracellularSequence Analysis
Transmembranei218 – 23720Helical; Name=S4 of repeat ISequence AnalysisAdd
BLAST
Topological domaini238 – 25215CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei253 – 27725Helical; Name=S5 of repeat ISequence AnalysisAdd
BLAST
Topological domaini278 – 387110ExtracellularSequence AnalysisAdd
BLAST
Transmembranei388 – 41326Helical; Name=S6 of repeat ISequence AnalysisAdd
BLAST
Topological domaini414 – 745332CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei746 – 77025Helical; Name=S1 of repeat IISequence AnalysisAdd
BLAST
Topological domaini771 – 78111ExtracellularSequence AnalysisAdd
BLAST
Transmembranei782 – 80524Helical; Name=S2 of repeat IISequence AnalysisAdd
BLAST
Topological domaini806 – 8138CytoplasmicSequence Analysis
Transmembranei814 – 83320Helical; Name=S3 of repeat IISequence AnalysisAdd
BLAST
Topological domaini834 – 8396ExtracellularSequence Analysis
Transmembranei840 – 86021Helical; Name=S4 of repeat IISequence AnalysisAdd
BLAST
Topological domaini861 – 87515CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei876 – 89621Helical; Name=S5 of repeat IISequence AnalysisAdd
BLAST
Topological domaini897 – 94953ExtracellularSequence AnalysisAdd
BLAST
Transmembranei950 – 97526Helical; Name=S6 of repeat IISequence AnalysisAdd
BLAST
Topological domaini976 – 1191216CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1192 – 121524Helical; Name=S1 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1216 – 122813ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1229 – 125426Helical; Name=S2 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1255 – 12606CytoplasmicSequence Analysis
Transmembranei1261 – 128222Helical; Name=S3 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1283 – 12864ExtracellularSequence Analysis
Transmembranei1287 – 130822Helical; Name=S4 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1309 – 132719CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1328 – 134922Helical; Name=S5 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1350 – 143586ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1436 – 146227Helical; Name=S6 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1463 – 151553CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1516 – 153924Helical; Name=S1 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1540 – 155011ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1551 – 157424Helical; Name=S2 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1575 – 15806CytoplasmicSequence Analysis
Transmembranei1581 – 160424Helical; Name=S3 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1605 – 161410ExtracellularSequence Analysis
Transmembranei1615 – 163622Helical; Name=S4 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1637 – 165115CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1652 – 167423Helical; Name=S5 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1675 – 173965ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1740 – 176425Helical; Name=S6 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1765 – 1978214CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon initial segment Source: BHF-UCL
  2. integral component of membrane Source: RGD
  3. node of Ranvier Source: BHF-UCL
  4. plasma membrane Source: RGD
  5. voltage-gated sodium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19781978Sodium channel protein type 8 subunit alphaPRO_0000390890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1356 – 13561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1370 – 13701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1381 – 13811N-linked (GlcNAc...)Sequence Analysis
Modified residuei1495 – 14951Phosphoserine; by PKCBy similarity

Post-translational modificationi

May be ubiquitinated by NEDD4L; which would promote its endocytosis.By similarity
Phosphorylation at Ser-1495 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88420.
PRIDEiO88420.

PTM databases

PhosphoSiteiO88420.

Expressioni

Tissue specificityi

Isoform 1 is highly expressed in brain, moderately in spinal cord, and at low levels in dorsal root ganglia, nodose ganglia and superior cervical ganglia. Not detected in sciatic nerve and non-neuronal tissues. Isoform 2 is hardly detectable, if at all, in brain, expressed at low levels in spinal cord and at highest levels in dorsal root ganglia.1 Publication

Gene expression databases

GenevestigatoriO88420.

Interactioni

Subunit structurei

Interacts with NEDD4 and NEDD4L (By similarity). Interacts with FGF13; may regulate SCN8A activity.By similarity1 Publication

Protein-protein interaction databases

BioGridi248327. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO88420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati114 – 442329IAdd
BLAST
Repeati733 – 1005273IIAdd
BLAST
Repeati1178 – 1493316IIIAdd
BLAST
Repeati1502 – 1799298IVAdd
BLAST
Domaini1893 – 192230IQPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231755.
HOVERGENiHBG053100.
InParanoidiO88420.
KOiK04840.
PhylomeDBiO88420.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR024583. DUF3451.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR008054. Na_channel_a8su.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
PfamiPF11933. DUF3451. 1 hit.
PF00520. Ion_trans. 4 hits.
PF00612. IQ. 1 hit.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSiPR00170. NACHANNEL.
PR01667. NACHANNEL8.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88420-1) [UniParc]FASTAAdd to Basket

Also known as: PN4

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARLLAPPG PDSFKPFTPE SLANIERRIA ESKLKKPPKA DGSHREDDED
60 70 80 90 100
SKPKPNSDLE AGKSLPFIYG DIPQGLVAVP LEDFDPYYLT QKTFVVLNRG
110 120 130 140 150
KTLFRFSATP ALYILSPFNL IRRIAIKILI HSVFSMIIMC TILTNCVFMT
160 170 180 190 200
FSNPPEWSKN VEYTFTGIYT FESLVKIIAR GFCIDGFTFL RDPWNWLDFS
210 220 230 240 250
VIMMAYVTEF VDLGNVSALR TFRVLRALKT ISVIPGLKTI VGALIQSVKK
260 270 280 290 300
LSDVMILTVF CLSVFALIGL QLFMGNLRNK CVVWPINFNE SYLENGTRGF
310 320 330 340 350
DWEEYINNKT NFYMVPGMLE PLLCGNSSDA GQCPEGFQCM KAGRNPNYGY
360 370 380 390 400
TSFDTFSWAF LALFRLMTQD YWENLYQLTL RAAGKTYMIF FVLVIFVGSF
410 420 430 440 450
YLVNLILAVV AMAYEEQNQA TLEEAEQKEA EFKAMLEQLK KQQEEAQAAA
460 470 480 490 500
MATSAGTVSE DAIEEEGEDG VGSPRSSSEL SKLSSKSAKE RRNRRKKRKQ
510 520 530 540 550
KELSEGEEKG DPEKVFKSES EDGMRRKAFR LPDNRIGRKF SIMNQSLLSI
560 570 580 590 600
PGSPFLSRHN SKSSIFSFRG PGRFRDPGSE NEFADDEHST VEESEGRRDS
610 620 630 640 650
LFIPIRARER RSSYSGYSGY SQCSRSSRIF PSLRRSVKRN STVDCNGVVS
660 670 680 690 700
LIGPGSHIGR LLPEATTEVE IKKKGPGSLL VSMDQLASYG RKDRINSIMS
710 720 730 740 750
VVTNTLVEEL EESQRKCPPC WYKFANTFLI WECHPYWIKL KEIVNLIVMD
760 770 780 790 800
PFVDLAITIC IVLNTLFMAM EHHPMTPQFE HVLAVGNLVF TGIFTAEMFL
810 820 830 840 850
KLIAMDPYYY FQEGWNIFDG FIVSLSLMEL SLADVEGLSV LRSFRLLRVF
860 870 880 890 900
KLAKSWPTLN MLIKIIGNSV GALGNLTLVL AIIVFIFAVV GMQLFGKSYK
910 920 930 940 950
ECVCKINQEC KLPRWHMNDF FHSFLIVFRV LCGEWIETMW DCMEVAGQAM
960 970 980 990 1000
CLIVFMMVMV IGNLVVLNLF LALLLSSFSA DNLAATDDDG EMNNLQISVI
1010 1020 1030 1040 1050
RIKKGVAWTK VKVHAFMQAH FKQREADEVK PLDELYEKKA NCIANHTGVD
1060 1070 1080 1090 1100
IHRNGDFQKN GNGTTSGIGS SVEKYIIDED HMSFINNPNL TVRVPIAVGE
1110 1120 1130 1140 1150
SDFENLNTED VSSESDPEGS KDKLDDTSSS EGSTIDIKPE VEEVPVEQPE
1160 1170 1180 1190 1200
EYLDPDACFT EGCVQRFKCC QVNIEEGLGK SWWILRKTCF LIVEHNWFET
1210 1220 1230 1240 1250
FIIFMILLSS GALAFEDIYI EQRKTIRTIL EYADKVFTYI FILEMLLKWT
1260 1270 1280 1290 1300
AYGFVKFFTN AWCWLDFLIV AVSLVSLIAN ALGYSELGAI KSLRTLRALR
1310 1320 1330 1340 1350
PLRALSRFEG MRVVVNALVG AIPSIMNVLL VCLIFWLIFS IMGVNLFAGK
1360 1370 1380 1390 1400
YHYCFNETSE IRFEIDIVNN KTDCEKLMEG NSTEIRWKNV KINFDNVGAG
1410 1420 1430 1440 1450
YLALLQVATF KGWMDIMYAA VDSRKPDEQP DYEGNIYMYI YFVIFIIFGS
1460 1470 1480 1490 1500
FFTLNLFIGV IIDNFNQQKK KFGGQDIFMT EEQKKYYNAM KKLGSKKPQK
1510 1520 1530 1540 1550
PIPRPLNKIQ GIVFDFVTQQ AFDIVIMMLI CLNMVTMMVE TDTQSKQMEN
1560 1570 1580 1590 1600
ILYWINLVFV IFFTCECVLK MFALRHYYFT IGWNIFDFVV VILSIVGMFL
1610 1620 1630 1640 1650
ADIIEKYFVS PTLFRVIRLA RIGRILRLIK GAKGIRTLLF ALMMSLPALF
1660 1670 1680 1690 1700
NIGLLLFLVM FIFSIFGMSN FAYVKHEAGI DDMFNFETFG NSMICLFQIT
1710 1720 1730 1740 1750
TSAGWDGLLL PILNRPPDCS LDKEHPGSGF KGDCGNPSVG IFFFVSYIII
1760 1770 1780 1790 1800
SFLIVVNMYI AIILENFSVA TEESADPLSE DDFETFYEIW EKFDPDATQF
1810 1820 1830 1840 1850
IEYCKLADFA DALEHPLRVP KPNTIELIAM DLPMVSGDRI HCLDILFAFT
1860 1870 1880 1890 1900
KRVLGDSGEL DILRQQMEER FVASNPSKVS YEPITTTLRR KQEEVSAVVL
1910 1920 1930 1940 1950
QRAYRGHLAR RGFICRKMAS NKLENGGTHR DKKESTPSTA SLPSYDSVTK
1960 1970
PDKEKQQRAE EGRRERAKRQ KEVRESKC
Length:1,978
Mass (Da):225,159
Last modified:November 1, 1998 - v1
Checksum:i9160843C5935B88B
GO
Isoform 2 (identifier: O88420-2) [UniParc]FASTAAdd to Basket

Also known as: PN4a

The sequence of this isoform differs from the canonical sequence as follows:
     664-664: E → EVKIDKAATDS

Note: May be due to competing donor splice site.

Show »
Length:1,988
Mass (Da):226,188
Checksum:iC1F7D87FDC266C8F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei664 – 6641E → EVKIDKAATDS in isoform 2. 1 PublicationVSP_038652

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049239 mRNA. Translation: AAC26014.1.
AF049240 mRNA. Translation: AAC26015.1.
RefSeqiNP_062139.2. NM_019266.2. [O88420-1]
UniGeneiRn.91216.

Genome annotation databases

GeneIDi29710.
KEGGirno:29710.
UCSCiRGD:3638. rat. [O88420-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049239 mRNA. Translation: AAC26014.1 .
AF049240 mRNA. Translation: AAC26015.1 .
RefSeqi NP_062139.2. NM_019266.2. [O88420-1 ]
UniGenei Rn.91216.

3D structure databases

ProteinModelPortali O88420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248327. 1 interaction.

Chemistry

BindingDBi O88420.
ChEMBLi CHEMBL2752.
GuidetoPHARMACOLOGYi 583.

PTM databases

PhosphoSitei O88420.

Proteomic databases

PaxDbi O88420.
PRIDEi O88420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29710.
KEGGi rno:29710.
UCSCi RGD:3638. rat. [O88420-1 ]

Organism-specific databases

CTDi 6334.
RGDi 3638. Scn8a.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231755.
HOVERGENi HBG053100.
InParanoidi O88420.
KOi K04840.
PhylomeDBi O88420.

Miscellaneous databases

NextBioi 610139.
PROi O88420.

Gene expression databases

Genevestigatori O88420.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR024583. DUF3451.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR008054. Na_channel_a8su.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view ]
Pfami PF11933. DUF3451. 1 hit.
PF00520. Ion_trans. 4 hits.
PF00612. IQ. 1 hit.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view ]
PRINTSi PR00170. NACHANNEL.
PR01667. NACHANNEL8.
SMARTi SM00015. IQ. 1 hit.
[Graphical view ]
PROSITEi PS50096. IQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Functional analysis of a voltage-gated sodium channel and its splice variant from rat dorsal root ganglia."
    Dietrich P.S., McGivern J.G., Delgado S.G., Koch B.D., Eglen R.M., Hunter J.C., Sangameswaran L.
    J. Neurochem. 70:2262-2272(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Tissue: Spinal ganglion.
  2. "Fibroblast growth factor homologous factor 2B: association with Nav1.6 and selective colocalization at nodes of Ranvier of dorsal root axons."
    Wittmack E.K., Rush A.M., Craner M.J., Goldfarb M., Waxman S.G., Dib-Hajj S.D.
    J. Neurosci. 24:6765-6775(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FGF13.

Entry informationi

Entry nameiSCN8A_RAT
AccessioniPrimary (citable) accession number: O88420
Secondary accession number(s): O88421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3