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O88420

- SCN8A_RAT

UniProt

O88420 - SCN8A_RAT

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Protein
Sodium channel protein type 8 subunit alpha
Gene
Scn8a
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi891 – 8988ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: UniProtKB
  3. sodium ion binding Source: RGD
  4. voltage-gated sodium channel activity Source: RGD

GO - Biological processi

  1. membrane depolarization during action potential Source: RefGenome
  2. myelination Source: BHF-UCL
  3. neuronal action potential Source: RGD
  4. peripheral nervous system development Source: BHF-UCL
  5. sodium ion transmembrane transport Source: GOC
  6. sodium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium channel protein type 8 subunit alpha
Alternative name(s):
Peripheral nerve protein type 4
Short name:
PN4
Sodium channel 6
Short name:
NaCh6
Sodium channel protein type VIII subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.6
Gene namesi
Name:Scn8a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3638. Scn8a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 127127Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei128 – 15124Helical; Name=S1 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini152 – 1598Extracellular Reviewed prediction
Transmembranei160 – 17920Helical; Name=S2 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini180 – 19213Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei193 – 21119Helical; Name=S3 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini212 – 2176Extracellular Reviewed prediction
Transmembranei218 – 23720Helical; Name=S4 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini238 – 25215Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei253 – 27725Helical; Name=S5 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini278 – 387110Extracellular Reviewed prediction
Add
BLAST
Transmembranei388 – 41326Helical; Name=S6 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini414 – 745332Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei746 – 77025Helical; Name=S1 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini771 – 78111Extracellular Reviewed prediction
Add
BLAST
Transmembranei782 – 80524Helical; Name=S2 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini806 – 8138Cytoplasmic Reviewed prediction
Transmembranei814 – 83320Helical; Name=S3 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini834 – 8396Extracellular Reviewed prediction
Transmembranei840 – 86021Helical; Name=S4 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini861 – 87515Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei876 – 89621Helical; Name=S5 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini897 – 94953Extracellular Reviewed prediction
Add
BLAST
Transmembranei950 – 97526Helical; Name=S6 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini976 – 1191216Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1192 – 121524Helical; Name=S1 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1216 – 122813Extracellular Reviewed prediction
Add
BLAST
Transmembranei1229 – 125426Helical; Name=S2 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1255 – 12606Cytoplasmic Reviewed prediction
Transmembranei1261 – 128222Helical; Name=S3 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1283 – 12864Extracellular Reviewed prediction
Transmembranei1287 – 130822Helical; Name=S4 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1309 – 132719Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1328 – 134922Helical; Name=S5 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1350 – 143586Extracellular Reviewed prediction
Add
BLAST
Transmembranei1436 – 146227Helical; Name=S6 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1463 – 151553Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1516 – 153924Helical; Name=S1 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1540 – 155011Extracellular Reviewed prediction
Add
BLAST
Transmembranei1551 – 157424Helical; Name=S2 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1575 – 15806Cytoplasmic Reviewed prediction
Transmembranei1581 – 160424Helical; Name=S3 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1605 – 161410Extracellular Reviewed prediction
Transmembranei1615 – 163622Helical; Name=S4 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1637 – 165115Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1652 – 167423Helical; Name=S5 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1675 – 173965Extracellular Reviewed prediction
Add
BLAST
Transmembranei1740 – 176425Helical; Name=S6 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1765 – 1978214Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. axon initial segment Source: BHF-UCL
  2. integral component of membrane Source: RGD
  3. node of Ranvier Source: BHF-UCL
  4. plasma membrane Source: RGD
  5. voltage-gated sodium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19781978Sodium channel protein type 8 subunit alpha
PRO_0000390890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi215 – 2151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi289 – 2891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi295 – 2951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi308 – 3081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi326 – 3261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1356 – 13561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1370 – 13701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1381 – 13811N-linked (GlcNAc...) Reviewed prediction
Modified residuei1495 – 14951Phosphoserine; by PKC By similarity

Post-translational modificationi

May be ubiquitinated by NEDD4L; which would promote its endocytosis By similarity.
Phosphorylation at Ser-1495 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88420.
PRIDEiO88420.

PTM databases

PhosphoSiteiO88420.

Expressioni

Tissue specificityi

Isoform 1 is highly expressed in brain, moderately in spinal cord, and at low levels in dorsal root ganglia, nodose ganglia and superior cervical ganglia. Not detected in sciatic nerve and non-neuronal tissues. Isoform 2 is hardly detectable, if at all, in brain, expressed at low levels in spinal cord and at highest levels in dorsal root ganglia.1 Publication

Gene expression databases

GenevestigatoriO88420.

Interactioni

Subunit structurei

Interacts with NEDD4 and NEDD4L By similarity. Interacts with FGF13; may regulate SCN8A activity.1 Publication

Protein-protein interaction databases

BioGridi248327. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO88420.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati114 – 442329I
Add
BLAST
Repeati733 – 1005273II
Add
BLAST
Repeati1178 – 1493316III
Add
BLAST
Repeati1502 – 1799298IV
Add
BLAST
Domaini1893 – 192230IQ
Add
BLAST

Sequence similaritiesi

Contains 1 IQ domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231755.
HOVERGENiHBG053100.
KOiK04840.
PhylomeDBiO88420.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR024583. DUF3451.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR008054. Na_channel_a8su.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
PfamiPF11933. DUF3451. 1 hit.
PF00520. Ion_trans. 4 hits.
PF00612. IQ. 1 hit.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSiPR00170. NACHANNEL.
PR01667. NACHANNEL8.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88420-1) [UniParc]FASTAAdd to Basket

Also known as: PN4

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAARLLAPPG PDSFKPFTPE SLANIERRIA ESKLKKPPKA DGSHREDDED     50
SKPKPNSDLE AGKSLPFIYG DIPQGLVAVP LEDFDPYYLT QKTFVVLNRG 100
KTLFRFSATP ALYILSPFNL IRRIAIKILI HSVFSMIIMC TILTNCVFMT 150
FSNPPEWSKN VEYTFTGIYT FESLVKIIAR GFCIDGFTFL RDPWNWLDFS 200
VIMMAYVTEF VDLGNVSALR TFRVLRALKT ISVIPGLKTI VGALIQSVKK 250
LSDVMILTVF CLSVFALIGL QLFMGNLRNK CVVWPINFNE SYLENGTRGF 300
DWEEYINNKT NFYMVPGMLE PLLCGNSSDA GQCPEGFQCM KAGRNPNYGY 350
TSFDTFSWAF LALFRLMTQD YWENLYQLTL RAAGKTYMIF FVLVIFVGSF 400
YLVNLILAVV AMAYEEQNQA TLEEAEQKEA EFKAMLEQLK KQQEEAQAAA 450
MATSAGTVSE DAIEEEGEDG VGSPRSSSEL SKLSSKSAKE RRNRRKKRKQ 500
KELSEGEEKG DPEKVFKSES EDGMRRKAFR LPDNRIGRKF SIMNQSLLSI 550
PGSPFLSRHN SKSSIFSFRG PGRFRDPGSE NEFADDEHST VEESEGRRDS 600
LFIPIRARER RSSYSGYSGY SQCSRSSRIF PSLRRSVKRN STVDCNGVVS 650
LIGPGSHIGR LLPEATTEVE IKKKGPGSLL VSMDQLASYG RKDRINSIMS 700
VVTNTLVEEL EESQRKCPPC WYKFANTFLI WECHPYWIKL KEIVNLIVMD 750
PFVDLAITIC IVLNTLFMAM EHHPMTPQFE HVLAVGNLVF TGIFTAEMFL 800
KLIAMDPYYY FQEGWNIFDG FIVSLSLMEL SLADVEGLSV LRSFRLLRVF 850
KLAKSWPTLN MLIKIIGNSV GALGNLTLVL AIIVFIFAVV GMQLFGKSYK 900
ECVCKINQEC KLPRWHMNDF FHSFLIVFRV LCGEWIETMW DCMEVAGQAM 950
CLIVFMMVMV IGNLVVLNLF LALLLSSFSA DNLAATDDDG EMNNLQISVI 1000
RIKKGVAWTK VKVHAFMQAH FKQREADEVK PLDELYEKKA NCIANHTGVD 1050
IHRNGDFQKN GNGTTSGIGS SVEKYIIDED HMSFINNPNL TVRVPIAVGE 1100
SDFENLNTED VSSESDPEGS KDKLDDTSSS EGSTIDIKPE VEEVPVEQPE 1150
EYLDPDACFT EGCVQRFKCC QVNIEEGLGK SWWILRKTCF LIVEHNWFET 1200
FIIFMILLSS GALAFEDIYI EQRKTIRTIL EYADKVFTYI FILEMLLKWT 1250
AYGFVKFFTN AWCWLDFLIV AVSLVSLIAN ALGYSELGAI KSLRTLRALR 1300
PLRALSRFEG MRVVVNALVG AIPSIMNVLL VCLIFWLIFS IMGVNLFAGK 1350
YHYCFNETSE IRFEIDIVNN KTDCEKLMEG NSTEIRWKNV KINFDNVGAG 1400
YLALLQVATF KGWMDIMYAA VDSRKPDEQP DYEGNIYMYI YFVIFIIFGS 1450
FFTLNLFIGV IIDNFNQQKK KFGGQDIFMT EEQKKYYNAM KKLGSKKPQK 1500
PIPRPLNKIQ GIVFDFVTQQ AFDIVIMMLI CLNMVTMMVE TDTQSKQMEN 1550
ILYWINLVFV IFFTCECVLK MFALRHYYFT IGWNIFDFVV VILSIVGMFL 1600
ADIIEKYFVS PTLFRVIRLA RIGRILRLIK GAKGIRTLLF ALMMSLPALF 1650
NIGLLLFLVM FIFSIFGMSN FAYVKHEAGI DDMFNFETFG NSMICLFQIT 1700
TSAGWDGLLL PILNRPPDCS LDKEHPGSGF KGDCGNPSVG IFFFVSYIII 1750
SFLIVVNMYI AIILENFSVA TEESADPLSE DDFETFYEIW EKFDPDATQF 1800
IEYCKLADFA DALEHPLRVP KPNTIELIAM DLPMVSGDRI HCLDILFAFT 1850
KRVLGDSGEL DILRQQMEER FVASNPSKVS YEPITTTLRR KQEEVSAVVL 1900
QRAYRGHLAR RGFICRKMAS NKLENGGTHR DKKESTPSTA SLPSYDSVTK 1950
PDKEKQQRAE EGRRERAKRQ KEVRESKC 1978
Length:1,978
Mass (Da):225,159
Last modified:November 1, 1998 - v1
Checksum:i9160843C5935B88B
GO
Isoform 2 (identifier: O88420-2) [UniParc]FASTAAdd to Basket

Also known as: PN4a

The sequence of this isoform differs from the canonical sequence as follows:
     664-664: E → EVKIDKAATDS

Note: May be due to competing donor splice site.

Show »
Length:1,988
Mass (Da):226,188
Checksum:iC1F7D87FDC266C8F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei664 – 6641E → EVKIDKAATDS in isoform 2.
VSP_038652

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF049239 mRNA. Translation: AAC26014.1.
AF049240 mRNA. Translation: AAC26015.1.
RefSeqiNP_062139.2. NM_019266.2. [O88420-1]
UniGeneiRn.91216.

Genome annotation databases

GeneIDi29710.
KEGGirno:29710.
UCSCiRGD:3638. rat. [O88420-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF049239 mRNA. Translation: AAC26014.1 .
AF049240 mRNA. Translation: AAC26015.1 .
RefSeqi NP_062139.2. NM_019266.2. [O88420-1 ]
UniGenei Rn.91216.

3D structure databases

ProteinModelPortali O88420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248327. 1 interaction.

Chemistry

BindingDBi O88420.
ChEMBLi CHEMBL2752.
GuidetoPHARMACOLOGYi 583.

PTM databases

PhosphoSitei O88420.

Proteomic databases

PaxDbi O88420.
PRIDEi O88420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29710.
KEGGi rno:29710.
UCSCi RGD:3638. rat. [O88420-1 ]

Organism-specific databases

CTDi 6334.
RGDi 3638. Scn8a.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231755.
HOVERGENi HBG053100.
KOi K04840.
PhylomeDBi O88420.

Miscellaneous databases

NextBioi 610139.
PROi O88420.

Gene expression databases

Genevestigatori O88420.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR024583. DUF3451.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR008054. Na_channel_a8su.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view ]
Pfami PF11933. DUF3451. 1 hit.
PF00520. Ion_trans. 4 hits.
PF00612. IQ. 1 hit.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view ]
PRINTSi PR00170. NACHANNEL.
PR01667. NACHANNEL8.
SMARTi SM00015. IQ. 1 hit.
[Graphical view ]
PROSITEi PS50096. IQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Functional analysis of a voltage-gated sodium channel and its splice variant from rat dorsal root ganglia."
    Dietrich P.S., McGivern J.G., Delgado S.G., Koch B.D., Eglen R.M., Hunter J.C., Sangameswaran L.
    J. Neurochem. 70:2262-2272(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Tissue: Spinal ganglion.
  2. "Fibroblast growth factor homologous factor 2B: association with Nav1.6 and selective colocalization at nodes of Ranvier of dorsal root axons."
    Wittmack E.K., Rush A.M., Craner M.J., Goldfarb M., Waxman S.G., Dib-Hajj S.D.
    J. Neurosci. 24:6765-6775(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FGF13.

Entry informationi

Entry nameiSCN8A_RAT
AccessioniPrimary (citable) accession number: O88420
Secondary accession number(s): O88421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi