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O88419

- B4GT6_RAT

UniProt

O88419 - B4GT6_RAT

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Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for the biosynthesis of glycosphingolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Manganese or magnesium or calcium.1 Publication

Enzyme regulationi

Inhibited by EDTA.

pH dependencei

Optimum pH is 7.2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi230 – 2301ManganeseBy similarity
Binding sitei258 – 2581UDP-alpha-D-galactoseBy similarity
Binding sitei290 – 2901UDP-alpha-D-galactoseBy similarity
Metal bindingi323 – 3231Manganese; via tele nitrogenBy similarity
Binding sitei334 – 3341N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: RGD

GO - Biological processi

  1. glycosphingolipid biosynthetic process Source: RGD
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 6 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 6
Short name:
Beta4Gal-T6
Short name:
b4Gal-T6
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
Including the following 1 domains:
Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274)
Alternative name(s):
Lactosylceramide synthase
Short name:
LacCer synthase
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi71046. B4galt6.

Subcellular locationi

Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack.By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Beta-1,4-galactosyltransferase 6PRO_0000080549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi108 ↔ 152By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi223 ↔ 242By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88419.
PRIDEiO88419.

Expressioni

Tissue specificityi

Highest expression in brain with lower levels found in lungs, heart, skeletal muscle and kidney. Lowest expression in testis, liver and spleen.1 Publication

Gene expression databases

GenevestigatoriO88419.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021764.

Structurei

3D structure databases

ProteinModelPortaliO88419.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicAdd
BLAST
Topological domaini36 – 382347LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3520Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1675UDP-alpha-D-galactose bindingBy similarity
Regioni202 – 2043UDP-alpha-D-galactose bindingBy similarity
Regioni229 – 2302UDP-alpha-D-galactose bindingBy similarity
Regioni292 – 2954N-acetyl-D-glucosamine bindingBy similarity
Regioni323 – 3242UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO88419.
KOiK07553.
OrthoDBiEOG7060R0.
PhylomeDBiO88419.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O88419-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG
60 70 80 90 100
IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF
110 120 130 140 150
TYSPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IEPGGHWRPQ
160 170 180 190 200
DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ
210 220 230 240 250
PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA
260 270 280 290 300
AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR
310 320 330 340 350
VHYSGYNVTR PEGDLGKYTS IPHHHRGEVQ FLGRYKLLRY SKERQFIDGL
360 370 380
NNLLYTPKIL VDRLYTNISV NLMPELAPVE DY
Length:382
Mass (Da):44,778
Last modified:November 1, 1998 - v1
Checksum:i37C2A32B32D10D51
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF048687 mRNA. Translation: AAC24515.1.
RefSeqiNP_113928.1. NM_031740.1.
UniGeneiRn.21126.

Genome annotation databases

GeneIDi65196.
KEGGirno:65196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF048687 mRNA. Translation: AAC24515.1 .
RefSeqi NP_113928.1. NM_031740.1.
UniGenei Rn.21126.

3D structure databases

ProteinModelPortali O88419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000021764.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

Proteomic databases

PaxDbi O88419.
PRIDEi O88419.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 65196.
KEGGi rno:65196.

Organism-specific databases

CTDi 9331.
RGDi 71046. B4galt6.

Phylogenomic databases

eggNOGi NOG327897.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi O88419.
KOi K07553.
OrthoDBi EOG7060R0.
PhylomeDBi O88419.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi 614134.
PROi O88419.

Gene expression databases

Genevestigatori O88419.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain."
    Nomura T., Takizawa M., Aoki J., Arai H., Inoue K., Wakisaka E., Yoshizuka N., Imokawa G., Dohmae N., Takio K., Hattori M., Matsuo N.
    J. Biol. Chem. 273:13570-13577(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 256-279; 318-336 AND 343-358, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiB4GT6_RAT
AccessioniPrimary (citable) accession number: O88419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3