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O88419

- B4GT6_RAT

UniProt

O88419 - B4GT6_RAT

Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Required for the biosynthesis of glycosphingolipids.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

    Cofactori

    Manganese or magnesium or calcium.1 Publication

    Enzyme regulationi

    Inhibited by EDTA.

    pH dependencei

    Optimum pH is 7.2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi230 – 2301ManganeseBy similarity
    Binding sitei258 – 2581UDP-alpha-D-galactoseBy similarity
    Binding sitei290 – 2901UDP-alpha-D-galactoseBy similarity
    Metal bindingi323 – 3231Manganese; via tele nitrogenBy similarity
    Binding sitei334 – 3341N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: RGD

    GO - Biological processi

    1. glycosphingolipid biosynthetic process Source: RGD
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Calcium, Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196475. Keratan sulfate biosynthesis.
    REACT_199227. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 6 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 6
    Short name:
    Beta4Gal-T6
    Short name:
    b4Gal-T6
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
    Including the following 1 domains:
    Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274)
    Alternative name(s):
    Lactosylceramide synthase
    Short name:
    LacCer synthase
    UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4galt6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi71046. B4galt6.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Beta-1,4-galactosyltransferase 6PRO_0000080549Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi108 ↔ 152By similarity
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi223 ↔ 242By similarity
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO88419.
    PRIDEiO88419.

    Expressioni

    Tissue specificityi

    Highest expression in brain with lower levels found in lungs, heart, skeletal muscle and kidney. Lowest expression in testis, liver and spleen.1 Publication

    Gene expression databases

    GenevestigatoriO88419.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000021764.

    Structurei

    3D structure databases

    ProteinModelPortaliO88419.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicAdd
    BLAST
    Topological domaini36 – 382347LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3520Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1675UDP-alpha-D-galactose bindingBy similarity
    Regioni202 – 2043UDP-alpha-D-galactose bindingBy similarity
    Regioni229 – 2302UDP-alpha-D-galactose bindingBy similarity
    Regioni292 – 2954N-acetyl-D-glucosamine bindingBy similarity
    Regioni323 – 3242UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiO88419.
    KOiK07553.
    OrthoDBiEOG7060R0.
    PhylomeDBiO88419.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O88419-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG    50
    IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF 100
    TYSPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IEPGGHWRPQ 150
    DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ 200
    PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA 250
    AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR 300
    VHYSGYNVTR PEGDLGKYTS IPHHHRGEVQ FLGRYKLLRY SKERQFIDGL 350
    NNLLYTPKIL VDRLYTNISV NLMPELAPVE DY 382
    Length:382
    Mass (Da):44,778
    Last modified:November 1, 1998 - v1
    Checksum:i37C2A32B32D10D51
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF048687 mRNA. Translation: AAC24515.1.
    RefSeqiNP_113928.1. NM_031740.1.
    UniGeneiRn.21126.

    Genome annotation databases

    GeneIDi65196.
    KEGGirno:65196.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF048687 mRNA. Translation: AAC24515.1 .
    RefSeqi NP_113928.1. NM_031740.1.
    UniGenei Rn.21126.

    3D structure databases

    ProteinModelPortali O88419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000021764.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    Proteomic databases

    PaxDbi O88419.
    PRIDEi O88419.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 65196.
    KEGGi rno:65196.

    Organism-specific databases

    CTDi 9331.
    RGDi 71046. B4galt6.

    Phylogenomic databases

    eggNOGi NOG327897.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi O88419.
    KOi K07553.
    OrthoDBi EOG7060R0.
    PhylomeDBi O88419.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196475. Keratan sulfate biosynthesis.
    REACT_199227. N-Glycan antennae elongation.

    Miscellaneous databases

    NextBioi 614134.
    PROi O88419.

    Gene expression databases

    Genevestigatori O88419.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain."
      Nomura T., Takizawa M., Aoki J., Arai H., Inoue K., Wakisaka E., Yoshizuka N., Imokawa G., Dohmae N., Takio K., Hattori M., Matsuo N.
      J. Biol. Chem. 273:13570-13577(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 256-279; 318-336 AND 343-358, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiB4GT6_RAT
    AccessioniPrimary (citable) accession number: O88419
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3