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Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the biosynthesis of glycosphingolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Mn2+1 Publication, Mg2+1 Publication, Ca2+1 Publication

Enzyme regulationi

Inhibited by EDTA.

pH dependencei

Optimum pH is 7.2.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi230ManganeseBy similarity1
Binding sitei258UDP-alpha-D-galactoseBy similarity1
Binding sitei290UDP-alpha-D-galactoseBy similarity1
Metal bindingi323Manganese; via tele nitrogenBy similarity1
Binding sitei334N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycosphingolipid biosynthetic process Source: RGD
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandCalcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378

Protein family/group databases

CAZyiGT7 Glycosyltransferase Family 7

Chemistry databases

SwissLipidsiSLP:000000764

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 6
Short name:
Beta-1,4-GalTase 6
Short name:
Beta4Gal-T6
Short name:
b4Gal-T6
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
Including the following 1 domains:
Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.2741 Publication)
Alternative name(s):
Lactosylceramide synthase
Short name:
LacCer synthase
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71046 B4galt6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicAdd BLAST15
Transmembranei16 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini36 – 382LumenalSequence analysisAdd BLAST347

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805491 – 382Beta-1,4-galactosyltransferase 6Add BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi108 ↔ 152By similarity
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi223 ↔ 242By similarity
Glycosylationi307N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88419
PRIDEiO88419

Expressioni

Tissue specificityi

Highest expression in brain with lower levels found in lungs, heart, skeletal muscle and kidney. Lowest expression in testis, liver and spleen.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021764

Structurei

3D structure databases

ProteinModelPortaliO88419
SMRiO88419
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 167UDP-alpha-D-galactose bindingBy similarity5
Regioni202 – 204UDP-alpha-D-galactose bindingBy similarity3
Regioni229 – 230UDP-alpha-D-galactose bindingBy similarity2
Regioni292 – 295N-acetyl-D-glucosamine bindingBy similarity4
Regioni323 – 324UDP-alpha-D-galactose bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916 Eukaryota
ENOG410ZYYA LUCA
HOGENOMiHOG000231027
HOVERGENiHBG058334
InParanoidiO88419
KOiK07553
PhylomeDBiO88419
TreeFamiTF312834

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR003859 Galactosyl_T
IPR027791 Galactosyl_T_C
IPR027995 Galactosyl_T_N
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR19300 PTHR19300, 1 hit
PfamiView protein in Pfam
PF02709 Glyco_transf_7C, 1 hit
PF13733 Glyco_transf_7N, 1 hit
PRINTSiPR02050 B14GALTRFASE
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

O88419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG
60 70 80 90 100
IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF
110 120 130 140 150
TYSPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IEPGGHWRPQ
160 170 180 190 200
DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ
210 220 230 240 250
PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA
260 270 280 290 300
AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR
310 320 330 340 350
VHYSGYNVTR PEGDLGKYTS IPHHHRGEVQ FLGRYKLLRY SKERQFIDGL
360 370 380
NNLLYTPKIL VDRLYTNISV NLMPELAPVE DY
Length:382
Mass (Da):44,778
Last modified:November 1, 1998 - v1
Checksum:i37C2A32B32D10D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048687 mRNA Translation: AAC24515.1
RefSeqiNP_113928.1, NM_031740.1
UniGeneiRn.21126

Genome annotation databases

GeneIDi65196
KEGGirno:65196

Similar proteinsi

Entry informationi

Entry nameiB4GT6_RAT
AccessioniPrimary (citable) accession number: O88419
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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