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O88419

- B4GT6_RAT

UniProt

O88419 - B4GT6_RAT

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Protein

Beta-1,4-galactosyltransferase 6

Gene
B4galt6
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the biosynthesis of glycosphingolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Manganese or magnesium or calcium.1 Publication

Enzyme regulationi

Inhibited by EDTA.

pH dependencei

Optimum pH is 7.2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi230 – 2301Manganese By similarity
Binding sitei258 – 2581UDP-alpha-D-galactose By similarity
Binding sitei290 – 2901UDP-alpha-D-galactose By similarity
Metal bindingi323 – 3231Manganese; via tele nitrogen By similarity
Binding sitei334 – 3341N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: RGD

GO - Biological processi

  1. glycosphingolipid biosynthetic process Source: RGD
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 6 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 6
Short name:
Beta4Gal-T6
Short name:
b4Gal-T6
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
Including the following 1 domains:
Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274)
Alternative name(s):
Lactosylceramide synthase
Short name:
LacCer synthase
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi71046. B4galt6.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicAdd
BLAST
Transmembranei16 – 3520Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini36 – 382347Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Beta-1,4-galactosyltransferase 6PRO_0000080549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi75 – 751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi83 – 831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi84 – 841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi108 ↔ 152 By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi223 ↔ 242 By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi367 – 3671N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88419.
PRIDEiO88419.

Expressioni

Tissue specificityi

Highest expression in brain with lower levels found in lungs, heart, skeletal muscle and kidney. Lowest expression in testis, liver and spleen.1 Publication

Gene expression databases

GenevestigatoriO88419.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021764.

Structurei

3D structure databases

ProteinModelPortaliO88419.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1675UDP-alpha-D-galactose binding By similarity
Regioni202 – 2043UDP-alpha-D-galactose binding By similarity
Regioni229 – 2302UDP-alpha-D-galactose binding By similarity
Regioni292 – 2954N-acetyl-D-glucosamine binding By similarity
Regioni323 – 3242UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO88419.
KOiK07553.
OrthoDBiEOG7060R0.
PhylomeDBiO88419.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O88419-1 [UniParc]FASTAAdd to Basket

« Hide

MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG    50
IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF 100
TYSPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IEPGGHWRPQ 150
DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ 200
PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA 250
AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR 300
VHYSGYNVTR PEGDLGKYTS IPHHHRGEVQ FLGRYKLLRY SKERQFIDGL 350
NNLLYTPKIL VDRLYTNISV NLMPELAPVE DY 382
Length:382
Mass (Da):44,778
Last modified:November 1, 1998 - v1
Checksum:i37C2A32B32D10D51
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF048687 mRNA. Translation: AAC24515.1.
RefSeqiNP_113928.1. NM_031740.1.
UniGeneiRn.21126.

Genome annotation databases

GeneIDi65196.
KEGGirno:65196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF048687 mRNA. Translation: AAC24515.1 .
RefSeqi NP_113928.1. NM_031740.1.
UniGenei Rn.21126.

3D structure databases

ProteinModelPortali O88419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000021764.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

Proteomic databases

PaxDbi O88419.
PRIDEi O88419.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 65196.
KEGGi rno:65196.

Organism-specific databases

CTDi 9331.
RGDi 71046. B4galt6.

Phylogenomic databases

eggNOGi NOG327897.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi O88419.
KOi K07553.
OrthoDBi EOG7060R0.
PhylomeDBi O88419.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi 614134.
PROi O88419.

Gene expression databases

Genevestigatori O88419.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain."
    Nomura T., Takizawa M., Aoki J., Arai H., Inoue K., Wakisaka E., Yoshizuka N., Imokawa G., Dohmae N., Takio K., Hattori M., Matsuo N.
    J. Biol. Chem. 273:13570-13577(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 256-279; 318-336 AND 343-358, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiB4GT6_RAT
AccessioniPrimary (citable) accession number: O88419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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