Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the biosynthesis of glycosphingolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Mn2+1 Publication, Mg2+1 Publication, Ca2+1 Publication

Enzyme regulationi

Inhibited by EDTA.

pH dependencei

Optimum pH is 7.2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi230 – 2301ManganeseBy similarity
Binding sitei258 – 2581UDP-alpha-D-galactoseBy similarity
Binding sitei290 – 2901UDP-alpha-D-galactoseBy similarity
Metal bindingi323 – 3231Manganese; via tele nitrogenBy similarity
Binding sitei334 – 3341N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycosphingolipid biosynthetic process Source: RGD
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 6 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 6
Short name:
Beta4Gal-T6
Short name:
b4Gal-T6
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
Including the following 1 domains:
Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274)
Alternative name(s):
Lactosylceramide synthase
Short name:
LacCer synthase
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71046. B4galt6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicAdd
BLAST
Transmembranei16 – 3520Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini36 – 382347LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Beta-1,4-galactosyltransferase 6PRO_0000080549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi108 ↔ 152By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi223 ↔ 242By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88419.
PRIDEiO88419.

Expressioni

Tissue specificityi

Highest expression in brain with lower levels found in lungs, heart, skeletal muscle and kidney. Lowest expression in testis, liver and spleen.1 Publication

Gene expression databases

GenevestigatoriO88419.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021764.

Structurei

3D structure databases

ProteinModelPortaliO88419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1675UDP-alpha-D-galactose bindingBy similarity
Regioni202 – 2043UDP-alpha-D-galactose bindingBy similarity
Regioni229 – 2302UDP-alpha-D-galactose bindingBy similarity
Regioni292 – 2954N-acetyl-D-glucosamine bindingBy similarity
Regioni323 – 3242UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO88419.
KOiK07553.
OrthoDBiEOG7060R0.
PhylomeDBiO88419.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O88419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG
60 70 80 90 100
IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF
110 120 130 140 150
TYSPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IEPGGHWRPQ
160 170 180 190 200
DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ
210 220 230 240 250
PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA
260 270 280 290 300
AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR
310 320 330 340 350
VHYSGYNVTR PEGDLGKYTS IPHHHRGEVQ FLGRYKLLRY SKERQFIDGL
360 370 380
NNLLYTPKIL VDRLYTNISV NLMPELAPVE DY
Length:382
Mass (Da):44,778
Last modified:November 1, 1998 - v1
Checksum:i37C2A32B32D10D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048687 mRNA. Translation: AAC24515.1.
RefSeqiNP_113928.1. NM_031740.1.
UniGeneiRn.21126.

Genome annotation databases

GeneIDi65196.
KEGGirno:65196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048687 mRNA. Translation: AAC24515.1.
RefSeqiNP_113928.1. NM_031740.1.
UniGeneiRn.21126.

3D structure databases

ProteinModelPortaliO88419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021764.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Proteomic databases

PaxDbiO88419.
PRIDEiO88419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65196.
KEGGirno:65196.

Organism-specific databases

CTDi9331.
RGDi71046. B4galt6.

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO88419.
KOiK07553.
OrthoDBiEOG7060R0.
PhylomeDBiO88419.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi614134.
PROiO88419.

Gene expression databases

GenevestigatoriO88419.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain."
    Nomura T., Takizawa M., Aoki J., Arai H., Inoue K., Wakisaka E., Yoshizuka N., Imokawa G., Dohmae N., Takio K., Hattori M., Matsuo N.
    J. Biol. Chem. 273:13570-13577(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 256-279; 318-336 AND 343-358, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiB4GT6_RAT
AccessioniPrimary (citable) accession number: O88419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.