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O88419 (B4GT6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 6

Short name=Beta-1,4-GalTase 6
Short name=Beta4Gal-T6
Short name=b4Gal-T6
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6

Including the following 1 domains:

  1. Glucosylceramide beta-1,4-galactosyltransferase
    EC=2.4.1.274
    Alternative name(s):
    Lactosylceramide synthase
    Short name=LacCer synthase
    UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene names
Name:B4galt6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the biosynthesis of glycosphingolipids. Ref.1

Catalytic activity

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide. Ref.1

Cofactor

Manganese or magnesium or calcium. Ref.1

Enzyme regulation

Inhibited by EDTA.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.

Tissue specificity

Highest expression in brain with lower levels found in lungs, heart, skeletal muscle and kidney. Lowest expression in testis, liver and spleen. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Beta-1,4-galactosyltransferase 6
PRO_0000080549

Regions

Topological domain1 – 1515Cytoplasmic
Transmembrane16 – 3520Helical; Signal-anchor for type II membrane protein; Potential
Topological domain36 – 382347Lumenal Potential
Region163 – 1675UDP-alpha-D-galactose binding By similarity
Region202 – 2043UDP-alpha-D-galactose binding By similarity
Region229 – 2302UDP-alpha-D-galactose binding By similarity
Region292 – 2954N-acetyl-D-glucosamine binding By similarity
Region323 – 3242UDP-alpha-D-galactose binding By similarity

Sites

Metal binding2301Manganese By similarity
Metal binding3231Manganese; via tele nitrogen By similarity
Binding site2581UDP-alpha-D-galactose By similarity
Binding site2901UDP-alpha-D-galactose By similarity
Binding site3341N-acetyl-D-glucosamine By similarity

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Disulfide bond108 ↔ 152 By similarity
Disulfide bond223 ↔ 242 By similarity

Sequences

Sequence LengthMass (Da)Tools
O88419 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 37C2A32B32D10D51

FASTA38244,778
        10         20         30         40         50         60 
MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG IMLRENVKTI 

        70         80         90        100        110        120 
GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF TYSPHLPCPE KLPYMRGFLS 

       130        140        150        160        170        180 
VNVSEISFDE VHQLFSKDSE IEPGGHWRPQ DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP 

       190        200        210        220        230        240 
MLQKQRLEFA FYVIEQTGTQ PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY 

       250        260        270        280        290        300 
GCGEMPRHFA AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR 

       310        320        330        340        350        360 
VHYSGYNVTR PEGDLGKYTS IPHHHRGEVQ FLGRYKLLRY SKERQFIDGL NNLLYTPKIL 

       370        380 
VDRLYTNISV NLMPELAPVE DY 

« Hide

References

[1]"Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain."
Nomura T., Takizawa M., Aoki J., Arai H., Inoue K., Wakisaka E., Yoshizuka N., Imokawa G., Dohmae N., Takio K., Hattori M., Matsuo N.
J. Biol. Chem. 273:13570-13577(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 256-279; 318-336 AND 343-358, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF048687 mRNA. Translation: AAC24515.1.
RefSeqNP_113928.1. NM_031740.1.
UniGeneRn.21126.

3D structure databases

ProteinModelPortalO88419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000021764.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Proteomic databases

PaxDbO88419.
PRIDEO88419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID65196.
KEGGrno:65196.

Organism-specific databases

CTD9331.
RGD71046. B4galt6.

Phylogenomic databases

eggNOGNOG327897.
HOGENOMHOG000231027.
HOVERGENHBG058334.
InParanoidO88419.
KOK07553.
OrthoDBEOG7060R0.
PhylomeDBO88419.
TreeFamTF312834.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

GenevestigatorO88419.

Family and domain databases

InterProIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
[Graphical view]
PANTHERPTHR19300. PTHR19300. 1 hit.
PfamPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
ProtoNetSearch...

Other

NextBio614134.
PROO88419.

Entry information

Entry nameB4GT6_RAT
AccessionPrimary (citable) accession number: O88419
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways