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Protein

Tubby-related protein 3

Gene

Tulp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the Shh signaling transduction pathway: recruited to primary cilia via association with the IFT complex A (IFT-A) and is required for recruitment of G protein-coupled receptor GPR161 to cilia, a promoter of PKA-dependent basal repression machinery in Shh signaling. Binds to phosphorylated inositide (phosphoinositide) lipids. Both IFT-A- and phosphoinositide-binding properties are required to regulate ciliary G protein-coupled receptor trafficking. Not involved in ciliogenesis.2 Publications

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • bone development Source: MGI
  • brain development Source: MGI
  • bronchus morphogenesis Source: MGI
  • central nervous system neuron differentiation Source: MGI
  • dorsal/ventral neural tube patterning Source: MGI
  • embryonic camera-type eye development Source: MGI
  • embryonic digit morphogenesis Source: MGI
  • embryonic neurocranium morphogenesis Source: MGI
  • ganglion development Source: MGI
  • limb development Source: MGI
  • negative regulation of smoothened signaling pathway Source: MGI
  • negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
  • negative regulation of smoothened signaling pathway involved in ventral spinal cord patterning Source: MGI
  • neural tube closure Source: MGI
  • neural tube development Source: MGI
  • neural tube formation Source: MGI
  • regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  • regulation of smoothened signaling pathway Source: MGI
  • smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

ReactomeiR-MMU-5610787. Hedgehog 'off' state.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubby-related protein 3
Alternative name(s):
Tubby-like protein 3
Gene namesi
Name:Tulp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1329045. Tulp3.

Subcellular locationi

  • Nucleus
  • Cell membrane
  • Cell projectioncilium
  • Cytoplasm
  • Secreted

  • Note: Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha (By similarity). Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.By similarity

GO - Cellular componenti

  • axoneme Source: MGI
  • ciliary base Source: MGI
  • cilium Source: MGI
  • cytoplasm Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-SubCell
  • intraciliary transport particle A Source: Ensembl
  • nucleolus Source: MGI
  • nucleus Source: UniProtKB
  • plasma membrane Source: MGI
  • primary cilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Membrane, Nucleus, Secreted

Pathology & Biotechi

Disruption phenotypei

Failure of neural tube closure and death by embryonic day 14.5. Failure of cranial neural tube closure coincident with increased neuroepithelial apoptosis specifically in the hindbrain and the caudal neural tube. In addition, the number of tubulin beta-3 positive cells is significantly decreased in the embryonic hindbrain. Morphological defects in the embryonic craniofacial regions, the spinal neural tube and the limbs.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Tubby-related protein 3PRO_0000186471Add
BLAST

Proteomic databases

EPDiO88413.
MaxQBiO88413.
PaxDbiO88413.
PRIDEiO88413.

PTM databases

iPTMnetiO88413.
PhosphoSiteiO88413.

Expressioni

Tissue specificityi

Widely expressed including eyes and adipose depots.1 Publication

Developmental stagei

Ubiquitously expressed during development.1 Publication

Gene expression databases

BgeeiO88413.
CleanExiMM_TULP3.
ExpressionAtlasiO88413. baseline and differential.
GenevisibleiO88413. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204384. 1 interaction.
STRINGi10090.ENSMUSP00000001562.

Structurei

3D structure databases

ProteinModelPortaliO88413.
SMRiO88413. Positions 202-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TUB family.Curated

Phylogenomic databases

eggNOGiKOG2502. Eukaryota.
ENOG410XQFT. LUCA.
GeneTreeiENSGT00610000085970.
HOGENOMiHOG000016044.
HOVERGENiHBG018010.
InParanoidiO88413.
OMAiAFEDETM.
OrthoDBiEOG77HDDQ.
PhylomeDBiO88413.
TreeFamiTF314076.

Family and domain databases

Gene3Di3.20.90.10. 1 hit.
InterProiIPR000007. Tubby_C.
IPR025659. Tubby_C-like.
IPR018066. Tubby_C_CS.
IPR005398. Tubby_N.
[Graphical view]
PfamiPF01167. Tub. 1 hit.
PF16322. Tub_N. 2 hits.
[Graphical view]
PRINTSiPR01573. SUPERTUBBY.
PR01574. TUBBYPROTEIN.
SUPFAMiSSF54518. SSF54518. 1 hit.
PROSITEiPS01200. TUB_1. 1 hit.
PS01201. TUB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAARCAPGP RGDSAFDDET LRLRQLKLDN QRALLEKKQR KKRLEPLMVQ
60 70 80 90 100
PNPEARLRRL KPRGSEEHTP LVDPQMPRSD VILHGIDGPA AFLKPEAQDL
110 120 130 140 150
ESKPQVLSVG SPAPEEGTEG SADGESPEET APKPDLQEIL QKHGILSSVN
160 170 180 190 200
YDEEPDKEED EGGNLSSPSA RSEESAAASQ KAASETGASG VTAQQGDAQL
210 220 230 240 250
GEVENLEDFA YSPAPRGVTV KCKVTRDKKG MDRGLFPTYY MHLEREENRK
260 270 280 290 300
IFLLAGRKRK KSKTSNYLVS TDPTDLSREG ESYIGKLRSN LMGTKFTVYD
310 320 330 340 350
HGVNPVKAQG LVEKAHTRQE LAAICYETNV LGFKGPRKMS VIIPGMNMNH
360 370 380 390 400
ERIPFRPRNE HESLLSKWQN KSMENLIELH NKAPVWNDDT QSYVLNFHGR
410 420 430 440 450
VTQASVKNFQ IVHGNDPDYI VMQFGRVADD VFTLDYNYPL CALQAFAIGL
460
SSFDSKLACE
Length:460
Mass (Da):51,231
Last modified:November 1, 1998 - v1
Checksum:i02EBC62899BB8B3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045582 mRNA. Translation: AAC95430.1.
AB016963 mRNA. Translation: BAA74752.1.
BC060068 mRNA. Translation: AAH60068.1.
CCDSiCCDS39647.1.
RefSeqiNP_035787.1. NM_011657.2.
UniGeneiMm.291783.
Mm.439490.

Genome annotation databases

EnsembliENSMUST00000001562; ENSMUSP00000001562; ENSMUSG00000001521.
GeneIDi22158.
KEGGimmu:22158.
UCSCiuc009edh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045582 mRNA. Translation: AAC95430.1.
AB016963 mRNA. Translation: BAA74752.1.
BC060068 mRNA. Translation: AAH60068.1.
CCDSiCCDS39647.1.
RefSeqiNP_035787.1. NM_011657.2.
UniGeneiMm.291783.
Mm.439490.

3D structure databases

ProteinModelPortaliO88413.
SMRiO88413. Positions 202-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204384. 1 interaction.
STRINGi10090.ENSMUSP00000001562.

PTM databases

iPTMnetiO88413.
PhosphoSiteiO88413.

Proteomic databases

EPDiO88413.
MaxQBiO88413.
PaxDbiO88413.
PRIDEiO88413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001562; ENSMUSP00000001562; ENSMUSG00000001521.
GeneIDi22158.
KEGGimmu:22158.
UCSCiuc009edh.1. mouse.

Organism-specific databases

CTDi7289.
MGIiMGI:1329045. Tulp3.

Phylogenomic databases

eggNOGiKOG2502. Eukaryota.
ENOG410XQFT. LUCA.
GeneTreeiENSGT00610000085970.
HOGENOMiHOG000016044.
HOVERGENiHBG018010.
InParanoidiO88413.
OMAiAFEDETM.
OrthoDBiEOG77HDDQ.
PhylomeDBiO88413.
TreeFamiTF314076.

Enzyme and pathway databases

ReactomeiR-MMU-5610787. Hedgehog 'off' state.

Miscellaneous databases

NextBioi302086.
PROiO88413.
SOURCEiSearch...

Gene expression databases

BgeeiO88413.
CleanExiMM_TULP3.
ExpressionAtlasiO88413. baseline and differential.
GenevisibleiO88413. MM.

Family and domain databases

Gene3Di3.20.90.10. 1 hit.
InterProiIPR000007. Tubby_C.
IPR025659. Tubby_C-like.
IPR018066. Tubby_C_CS.
IPR005398. Tubby_N.
[Graphical view]
PfamiPF01167. Tub. 1 hit.
PF16322. Tub_N. 2 hits.
[Graphical view]
PRINTSiPR01573. SUPERTUBBY.
PR01574. TUBBYPROTEIN.
SUPFAMiSSF54518. SSF54518. 1 hit.
PROSITEiPS01200. TUB_1. 1 hit.
PS01201. TUB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a novel tubby gene family member, TULP3, in mouse and humans."
    Nishina P.M., North M.A., Ikeda A., Yan Y., Naggert J.K.
    Genomics 54:215-220(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cJ.
  2. Shirayoshi Y., Kawamura A., Nakatsuji N.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Neural tube defects and neuroepithelial cell death in Tulp3 knockout mice."
    Ikeda A., Ikeda S., Gridley T., Nishina P.M., Naggert J.K.
    Hum. Mol. Genet. 10:1325-1334(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  5. "Tulp3 is a critical repressor of mouse hedgehog signaling."
    Cameron D.A., Pennimpede T., Petkovich M.
    Dev. Dyn. 238:1140-1149(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE SHH PATHWAY, DISRUPTION PHENOTYPE.
  6. "Unconventional secretion of tubby and tubby-like protein 1."
    Caberoy N.B., Li W.
    FEBS Lett. 583:3057-3062(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Tubby-like protein 3 (TULP3) regulates patterning in the mouse embryo through inhibition of Hedgehog signaling."
    Norman R.X., Ko H.W., Huang V., Eun C.M., Abler L.L., Zhang Z., Sun X., Eggenschwiler J.T.
    Hum. Mol. Genet. 18:1740-1754(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE SHH PATHWAY, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
  8. "Intraflagellar transport protein 122 antagonizes Sonic Hedgehog signaling and controls ciliary localization of pathway components."
    Qin J., Lin Y., Norman R.X., Ko H.W., Eggenschwiler J.T.
    Proc. Natl. Acad. Sci. U.S.A. 108:1456-1461(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTULP3_MOUSE
AccessioniPrimary (citable) accession number: O88413
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.