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Protein

Mothers against decapentaplegic homolog 7

Gene

Smad7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi125ZincBy similarity1
Metal bindingi180ZincBy similarity1
Metal bindingi192ZincBy similarity1
Metal bindingi197ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 7
Short name:
MAD homolog 7
Short name:
Mothers against DPP homolog 7
Alternative name(s):
SMAD family member 7
Short name:
SMAD 7
Short name:
Smad7
Gene namesi
Name:Smad7
Synonyms:Madh7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69314 Smad7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000908741 – 426Mothers against decapentaplegic homolog 7Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64N6-acetyllysine; alternateBy similarity1
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei70N6-acetyllysine; alternateBy similarity1
Cross-linki70Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei249PhosphoserinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1 (By similarity).By similarity
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation (By similarity).By similarity
Acetylation prevents ubiquitination and degradation mediated by SMURF1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88406
PRIDEiO88406

PTM databases

PhosphoSitePlusiO88406

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Interacts with COPS5. Interacts with STAMBP. Interacts with NEDD4L. Interacts with RNF111, AXIN1 and AXIN2. Interacts with PPP1R15A. Interacts with ACVR1B, SMURF1, SMURF2 and TGFBR1; SMAD7 recruits SMURF1 and SMURF2 to the TGF-beta receptor and regulates its degradation. Interacts with WWP1 (By similarity). Interacts with PDPK1 (via PH domain) (By similarity).By similarity

Protein-protein interaction databases

BioGridi249513, 3 interactors
IntActiO88406, 1 interactor
MINTiO88406
STRINGi10116.ENSRNOP00000024831

Structurei

3D structure databases

ProteinModelPortaliO88406
SMRiO88406
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 207MH1PROSITE-ProRule annotationAdd BLAST144
Domaini261 – 426MH2PROSITE-ProRule annotationAdd BLAST166

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni208 – 217Important for interaction with SMURF2By similarity10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi208 – 211PY-motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 36Poly-Gly10
Compositional biasi49 – 56Poly-Gly8
Compositional biasi206 – 209Poly-Pro4

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGiKOG3701 Eukaryota
ENOG410XQKU LUCA
HOGENOMiHOG000060106
HOVERGENiHBG053021
InParanoidiO88406
KOiK19631
PhylomeDBiO88406
TreeFamiTF314923

Family and domain databases

Gene3Di2.60.200.10, 1 hit
3.90.520.10, 1 hit
InterProiView protein in InterPro
IPR013790 Dwarfin
IPR003619 MAD_homology1_Dwarfin-type
IPR013019 MAD_homology_MH1
IPR017855 SMAD-like_dom_sf
IPR001132 SMAD_dom_Dwarfin-type
IPR008984 SMAD_FHA_dom_sf
IPR036578 SMAD_MH1_sf
PANTHERiPTHR13703 PTHR13703, 1 hit
PfamiView protein in Pfam
PF03165 MH1, 1 hit
PF03166 MH2, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
SM00524 DWB, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56366 SSF56366, 2 hits
PROSITEiView protein in PROSITE
PS51075 MH1, 1 hit
PS51076 MH2, 1 hit

Sequencei

Sequence statusi: Complete.

O88406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRTKRSALV RRLWRSRAPG GEDEEEGVGG GGGGGGLRGE GATDGRAYGA
60 70 80 90 100
GGGGAGRAGC CLGKAVRGAK GHHHPHPPSS GAGAAGGAEA DLKALTHSVL
110 120 130 140 150
KKLKERQLEL LLQAVESRGG TRTACLLLPG RLDCRLGPGA PASAQPAQPP
160 170 180 190 200
SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC ESYGKINPEL VCCNPHHLSR
210 220 230 240 250
LCELESPPPP YSRYPMDFLK PTADCPDAVP SSDETGGTNY LAPGGLSDSQ
260 270 280 290 300
LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ
310 320 330 340 350
LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP
360 370 380 390 400
DSRTLLVHKV FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV
410 420
KGWGQCYTRQ FISSCPCWLE VIFNSR
Length:426
Mass (Da):46,458
Last modified:May 4, 2001 - v2
Checksum:i135B7D34972B455D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36G → D in AAF00608 (PubMed:11181520).Curated1
Sequence conflicti36G → D in AAD41130 (PubMed:11181520).Curated1
Sequence conflicti193Missing in AAC25062 (PubMed:10656934).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156730
, AF156727, AF156728, AF156729 Genomic DNA Translation: AAF00608.1
AF159626 mRNA Translation: AAD41130.1
AF042499 mRNA Translation: AAC25062.1
RefSeqiNP_110485.1, NM_030858.1
UniGeneiRn.29980

Genome annotation databases

GeneIDi81516
KEGGirno:81516

Similar proteinsi

Entry informationi

Entry nameiSMAD7_RAT
AccessioniPrimary (citable) accession number: O88406
Secondary accession number(s): Q9QUG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: May 23, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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