Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mothers against decapentaplegic homolog 7

Gene

Smad7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi125 – 1251ZincBy similarity
Metal bindingi180 – 1801ZincBy similarity
Metal bindingi192 – 1921ZincBy similarity
Metal bindingi197 – 1971ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 7
Short name:
MAD homolog 7
Short name:
Mothers against DPP homolog 7
Alternative name(s):
SMAD family member 7
Short name:
SMAD 7
Short name:
Smad7
Gene namesi
Name:Smad7
Synonyms:Madh7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69314. Smad7.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Mothers against decapentaplegic homolog 7PRO_0000090874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641N6-acetyllysine; alternateBy similarity
Cross-linki64 – 64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei70 – 701N6-acetyllysine; alternateBy similarity
Cross-linki70 – 70Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei249 – 2491PhosphoserinePROSITE-ProRule annotationBy similarity

Post-translational modificationi

Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription. Phosphorylated by PDPK1.By similarity
Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation (By similarity).By similarity
Acetylation prevents ubiquitination and degradation mediated by SMURF1. Ubiquitinated by WWP1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88406.

PTM databases

PhosphoSiteiO88406.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSRNOG00000018359.

Interactioni

Subunit structurei

Interacts with COPS5. Interacts with STAMBP. Interacts with NEDD4L. Interacts with RNF111, AXIN1 and AXIN2. Interacts with PPP1R15A. Interacts with ACVR1B, SMURF1, SMURF2 and TGFBR1; SMAD7 recruits SMURF1 and SMURF2 to the TGF-beta receptor and regulates its degradation. Interacts with WWP1 (By similarity). Interacts with PDPK1 (via PH domain) (By similarity).By similarity

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: RGD

Protein-protein interaction databases

BioGridi249513. 3 interactions.
IntActiO88406. 1 interaction.
MINTiMINT-2739720.
STRINGi10116.ENSRNOP00000024831.

Structurei

3D structure databases

ProteinModelPortaliO88406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 207144MH1PROSITE-ProRule annotationAdd
BLAST
Domaini261 – 426166MH2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni208 – 21710Important for interaction with SMURF2By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi208 – 2114PY-motifBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi27 – 3610Poly-Gly
Compositional biasi49 – 568Poly-Gly
Compositional biasi206 – 2094Poly-Pro

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated
Contains 1 MH1 (MAD homology 1) domain.PROSITE-ProRule annotation
Contains 1 MH2 (MAD homology 2) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
HOGENOMiHOG000060106.
HOVERGENiHBG053021.
InParanoidiO88406.
KOiK19631.
PhylomeDBiO88406.
TreeFamiTF314923.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 2 hits.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 2 hits.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 2 hits.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRTKRSALV RRLWRSRAPG GEDEEEGVGG GGGGGGLRGE GATDGRAYGA
60 70 80 90 100
GGGGAGRAGC CLGKAVRGAK GHHHPHPPSS GAGAAGGAEA DLKALTHSVL
110 120 130 140 150
KKLKERQLEL LLQAVESRGG TRTACLLLPG RLDCRLGPGA PASAQPAQPP
160 170 180 190 200
SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC ESYGKINPEL VCCNPHHLSR
210 220 230 240 250
LCELESPPPP YSRYPMDFLK PTADCPDAVP SSDETGGTNY LAPGGLSDSQ
260 270 280 290 300
LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ
310 320 330 340 350
LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP
360 370 380 390 400
DSRTLLVHKV FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV
410 420
KGWGQCYTRQ FISSCPCWLE VIFNSR
Length:426
Mass (Da):46,458
Last modified:May 4, 2001 - v2
Checksum:i135B7D34972B455D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361G → D in AAF00608 (PubMed:11181520).Curated
Sequence conflicti36 – 361G → D in AAD41130 (PubMed:11181520).Curated
Sequence conflicti193 – 1931Missing in AAC25062 (PubMed:10656934).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156730
, AF156727, AF156728, AF156729 Genomic DNA. Translation: AAF00608.1.
AF159626 mRNA. Translation: AAD41130.1.
AF042499 mRNA. Translation: AAC25062.1.
RefSeqiNP_110485.1. NM_030858.1.
UniGeneiRn.29980.

Genome annotation databases

GeneIDi81516.
KEGGirno:81516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156730
, AF156727, AF156728, AF156729 Genomic DNA. Translation: AAF00608.1.
AF159626 mRNA. Translation: AAD41130.1.
AF042499 mRNA. Translation: AAC25062.1.
RefSeqiNP_110485.1. NM_030858.1.
UniGeneiRn.29980.

3D structure databases

ProteinModelPortaliO88406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249513. 3 interactions.
IntActiO88406. 1 interaction.
MINTiMINT-2739720.
STRINGi10116.ENSRNOP00000024831.

PTM databases

PhosphoSiteiO88406.

Proteomic databases

PaxDbiO88406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81516.
KEGGirno:81516.

Organism-specific databases

CTDi4092.
RGDi69314. Smad7.

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
HOGENOMiHOG000060106.
HOVERGENiHBG053021.
InParanoidiO88406.
KOiK19631.
PhylomeDBiO88406.
TreeFamiTF314923.

Miscellaneous databases

PROiO88406.

Gene expression databases

BgeeiENSRNOG00000018359.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 2 hits.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 2 hits.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 2 hits.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMAD7_RAT
AccessioniPrimary (citable) accession number: O88406
Secondary accession number(s): Q9QUG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.