O88393 (TGBR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transforming growth factor beta receptor type 3
Short name=TGF-beta receptor type 3
Short name=TGFR-3

Alternative name(s):
Betaglycan
Transforming growth factor beta receptor III
Short name=TGF-beta receptor type III

Gene names

Name:

Tgfbr3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	850 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors By similarity.
Subunit structure	Interacts with TCTEX1D4 By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Secreted By similarity. Secreted › extracellular space › extracellular matrix By similarity. Note: Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix By similarity.
Post-translational modification	Extensively modified by glycosaminoglycan (GAG), either chondroitin sulfate or heparan sulfate depending upon the tissue of origin.
Sequence similarities	Contains 1 ZP domain.

Ontologies

Keywords
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Proteoglycan
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from electronic annotation. Source: Compara blastocyst development Inferred from direct assay PubMed 9921650. Source: MGI cardiac epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara cardiac muscle cell proliferation Inferred from mutant phenotype PubMed 12773577. Source: BHF-UCL cell growth Inferred from electronic annotation. Source: Compara definitive erythrocyte differentiation Inferred from mutant phenotype PubMed 12773577. Source: BHF-UCL epicardial cell to mesenchymal cell transition Traceable author statement PubMed 20299672. Source: DFLAT heart trabecula formation Inferred from mutant phenotype PubMed 12773577. Source: BHF-UCL immune response Inferred from electronic annotation. Source: Compara intracellular protein kinase cascade Inferred from electronic annotation. Source: Compara liver development Inferred from mutant phenotype PubMed 12773577. Source: BHF-UCL negative regulation of epithelial cell migration Inferred from mutant phenotype PubMed 17823118. Source: MGI negative regulation of epithelial cell proliferation Inferred from mutant phenotype PubMed 17295310. Source: BHF-UCL negative regulation of epithelial to mesenchymal transition Inferred from mutant phenotype PubMed 17823118. Source: MGI negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara organ regeneration Inferred from electronic annotation. Source: Compara palate development Inferred from mutant phenotype PubMed 17295310. Source: BHF-UCL pathway-restricted SMAD protein phosphorylation Inferred from mutant phenotype PubMed 17295310. Source: BHF-UCL positive regulation of NF-kappaB transcription factor activity Inferred from mutant phenotype PubMed 17823118. Source: MGI positive regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of blood vessel size Traceable author statement PubMed 20299672. Source: DFLAT regulation of protein binding Inferred from electronic annotation. Source: Compara response to follicle-stimulating hormone stimulus Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to luteinizing hormone stimulus Inferred from electronic annotation. Source: Compara response to prostaglandin E stimulus Inferred from electronic annotation. Source: Compara transforming growth factor beta receptor complex assembly Inferred from electronic annotation. Source: Compara transforming growth factor beta receptor signaling pathway Inferred from direct assay PubMed 11546783PubMed 17295310. Source: BHF-UCL vasculogenesis involved in coronary vascular morphogenesis Traceable author statement PubMed 20299672. Source: DFLAT ventricular cardiac muscle tissue morphogenesis Inferred from mutant phenotype PubMed 12773577. Source: BHF-UCL visceral serous pericardium development Traceable author statement PubMed 20299672. Source: DFLAT
Cellular_component	cell surface Inferred from direct assay PubMed 11546783. Source: BHF-UCL endoplasmic reticulum Inferred from direct assay PubMed 9921650. Source: MGI external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara inhibin-betaglycan-ActRII complex Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement PubMed 20299672. Source: DFLAT proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	SMAD binding Inferred from mutant phenotype PubMed 17295310. Source: BHF-UCL coreceptor activity Inferred from electronic annotation. Source: Compara glycosaminoglycan binding Inferred from direct assay PubMed 12773577. Source: BHF-UCL heparin binding Inferred from electronic annotation. Source: Compara transforming growth factor beta binding Inferred from direct assay PubMed 17295310. Source: BHF-UCL transforming growth factor beta receptor activity, type III Inferred from electronic annotation. Source: Compara transforming growth factor beta-activated receptor activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Chain

23 – 850

828

Transforming growth factor beta receptor type 3

PRO_0000041664

Regions

Topological domain

23 – 785

763

Extracellular Potential

Transmembrane

786 – 808

23

Helical; Potential

Topological domain

809 – 850

42

Cytoplasmic Potential

Domain

454 – 728

275

ZP

Amino acid modifications

Glycosylation

143

1

N-linked (GlcNAc...) Potential

Glycosylation

491

1

N-linked (GlcNAc...) Potential

Glycosylation

533

1

O-linked (Xyl...) (glycosaminoglycan) Ref.1

Glycosylation

544

1

O-linked (Xyl...) (glycosaminoglycan) Ref.1

Glycosylation

570

1

N-linked (GlcNAc...) Potential

Glycosylation

589

1

N-linked (GlcNAc...) Potential

Glycosylation

696

1

N-linked (GlcNAc...) Potential

Disulfide bond

638 ↔ 704

By similarity

Disulfide bond

659 ↔ 728

By similarity

Experimental info

Mutagenesis

533

1

S → A: Loss of glycosaminoglycan chains; when associated with A-544. Ref.1

Mutagenesis

544

1

S → A: Loss of glycosaminoglycan chains; when associated with A-533. Ref.1

Sequence conflict

322

1

Y → I in AAH70428. Ref.2

Sequence conflict

391

1

P → S in AAH70428. Ref.2

Sequence conflict

712

1

N → K in AAH70428. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

850

Helix Strand Turn

Details...

Beta strand

592 – 599

8

Beta strand

607 – 612

6

Beta strand

617 – 627

11

Beta strand

629 – 644

16

Beta strand

646 – 648

3

Beta strand

653 – 656

4

Beta strand

666 – 675

10

Turn

676 – 679

4

Beta strand

680 – 690

11

Beta strand

695 – 714

20

Turn

724 – 728

5

Beta strand

747 – 756

10

Sequences

	Sequence		Length	Mass (Da)
	O88393 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 4154D034C7307C86	FASTA	850	93,829
	10 20 30 40 50 60 MAVTSHHMVP VFVLMSACLA TAGPEPSTRC ELSPISASHP VQALMESFTV LSGCASRGTT 70 80 90 100 110 120 GLPREVHILN LRSTDQGLGQ PQREVTLHLN PIASVHTHHK PVVFLLNSPQ PLVWHVKTER 130 140 150 160 170 180 LAAGVPRLFL VSEGSVVQFS SGNFSLTAET EERSFPQENE HLLHWAQKEY GAVTSFTELK 190 200 210 220 230 240 IARNIYIKVG EDQVFPPTCN IGKNFLSLNY LAEYLQPKAA EGCVLASQPH EKEVHIIELI 250 260 270 280 290 300 SPNSNPYSTF QVDIIIDIRP AREDPEVVKN LVLILKCKKS VNWVIKSFDV KGNLKVIAPD 310 320 330 340 350 360 SIGFGKESER SMTVTKLVRN DYPSTQENLM KWALDNGYSP VTSYTIAPVA NRFHLRLENN 370 380 390 400 410 420 EEMRDEEVHT IPPELRILLG PDHLPALDSP PFQGEIPNGG FPFPFPDIPR RGWKEGEDRI 430 440 450 460 470 480 PRPKEPIIPR VQLLPDHREP EEVQGGVNIA LSVKCDNEKM VVAVDKDSFQ TNGYSGMELT 490 500 510 520 530 540 LLDPSCKAKM NGTHFVLESP LNGCGTRHRR SAPDGVVYYN SIVVQAPSPG DSSGWPDGYE 550 560 570 580 590 600 DLESGDNGFP GDTDEGETAP LSRAGVVVFN CSLRQLRSPS GFQDQLDGNA TFNMELYNTD 610 620 630 640 650 660 LFLVPSPGVF SVAENEHVYV EVSVTKADQD LGFAIQTCFI SPYSNPDRMS DYTIIENICP 670 680 690 700 710 720 KDDSVKFYSS KRVHFPIPHA EVDKKRFSFV FKSVFNTSLL FLHCELTLCS RNKGSQKLPK 730 740 750 760 770 780 CVTPDDACTS LDATMIWTMM QNKKTFTKPL AVVLQVDYKE NVPNMKESSP VPPPPQIFHG 790 800 810 820 830 840 LDTLTVMGIA FAAFVIGALL TGALWYIYSH TGETARRQQV PTSPPASENS SAAHSIGSTQ 850 STPCSSSSTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Murine betaglycan primary structure, expression and glycosaminoglycan attachment sites." Ponce-Castaneda M.V., Esparza-Lopez J., Vilchis-Landeros M.M., Mendoza V., Lopez-Casillas F. Biochim. Biophys. Acta 1384:189-196(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION AT SER-533 AND SER-544, MUTAGENESIS OF SER-533 AND SER-544.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF039601 mRNA. Translation: AAC28564.1.
BC070428 mRNA. Translation: AAH70428.1.

IPI

IPI00314779.

RefSeq

NP_035708.2. NM_011578.3.

UniGene

Mm.200775.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AJV	X-ray	2.70	A	591-757	[»]

ProteinModelPortal

O88393.

SMR

O88393. Positions 587-755.

ModBase

Search...

PTM databases

PhosphoSite

O88393.

Proteomic databases

PRIDE

O88393.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000031224; ENSMUSP00000031224; ENSMUSG00000029287.

GeneID

21814.

KEGG

mmu:21814.

UCSC

uc008ylz.2. mouse.

Organism-specific databases

CTD

7049.

MGI

MGI:104637. Tgfbr3.

Phylogenomic databases

eggNOG

NOG40778.

GeneTree

ENSGT00530000063861.

HOGENOM

HOG000090193.

HOVERGEN

HBG057515.

InParanoid

O88393.

KO

K05843.

OrthoDB

EOG4PVNXW.

Gene expression databases

Bgee

O88393.

CleanEx

MM_TGFBR3.

Genevestigator

O88393.

GermOnline

ENSMUSG00000029287. Mus musculus.

Family and domain databases

InterPro

IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]

Pfam

PF00100. Zona_pellucida. 1 hit.
[Graphical view]

PRINTS

PR00023. ZPELLUCIDA.

SMART

SM00241. ZP. 1 hit.
[Graphical view]

PROSITE

PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

301212.

SOURCE

Search...

Entry information

Entry name

TGBR3_MOUSE

Accession

Primary (citable) accession number: O88393
Secondary accession number(s): Q6NS72

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1998
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program