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O88387 (FGD4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FYVE, RhoGEF and PH domain-containing protein 4
Alternative name(s):
Actin filament-binding protein frabin
FGD1-related F-actin-binding protein
Gene names
Name:Fgd4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length766 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8. Ref.1 Ref.2

Subunit structure

Homooligomer. Ref.1

Subcellular location

Cytoplasmcytoskeleton. Cell projectionfilopodium. Note: Concentrated in filopodia and poorly detected at lamellipodia. Binds along the sides of actin fibers. Ref.2

Tissue specificity

Detected in brain, lung, liver, skeletal muscle, kidney, testis and cultured hippocampal neurons. Ref.2

Domain

The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain. Ref.2

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 FYVE-type zinc finger.

Contains 2 PH domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 766766FYVE, RhoGEF and PH domain-containing protein 4
PRO_0000080949

Regions

Domain206 – 393188DH
Domain422 – 521100PH 1
Domain643 – 74098PH 2
Zinc finger559 – 61961FYVE-type
Region1 – 150150Actin filament-binding

Sequences

Sequence LengthMass (Da)Tools
O88387 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: E34831DC0B0B334C

FASTA76686,454
        10         20         30         40         50         60 
MEESNPAPTS CASKGKHSKV SDLISHFEGG SVLSSYTDVQ KDSTMNLNIP QTPRQHGLTS 

        70         80         90        100        110        120 
TTPQKLPSHK SPQKQEKDSD QNQGQHGCLA NGVAAAQSQM ECETEKEAAL SPETDTQTAA 

       130        140        150        160        170        180 
ASPDAHVLNG VRNETTTDSA SSVTNSHDEN ACDSSCRTQG TDLGLPSKEG EPVIEAELQE 

       190        200        210        220        230        240 
RENGLSTEGL NPLDQHHEVK ETNEQKLHKI ATELLLTERA YVSRLNLLDQ VFYCKLLEEA 

       250        260        270        280        290        300 
NRGSFPAEMV NKIFSNISSI NAFHSKFLLP ELEKRMQEWE TTPRIGDILQ KLAPFLKMYG 

       310        320        330        340        350        360 
EYVKGFDNAV ELVKNMTERV PQFKSVTEEI QKQKICGSLT LQHHMLEPIQ RIPRYEMLLK 

       370        380        390        400        410        420 
DYLKKLSPDA PDWNDAKKSL EIISTAASHS NSAIRKMENL KKLLEIYEML GEEEDIVNPS 

       430        440        450        460        470        480 
NELIKEGQIL KLAARNTSAQ ERYLFLFNNM LLYCVPRFSL VGSKFTVRTR VGIDGMKIVE 

       490        500        510        520        530        540 
THNEEYPHTF QVSGKERTLE LQASSEQDKE EWIKALQESI DAFHQRHETF RNAIAKENDI 

       550        560        570        580        590        600 
PLEVSTAELG KRAPRWIRDN EVTMCMKCKE SFNALTRRRH HCRACGHVVC WKCSDYKAQL 

       610        620        630        640        650        660 
EYDGGRLNKV CKDCYQIMSG FAESEEKKRR GILEIESAEV SGNSEVCSFL QYMEKSKPWQ 

       670        680        690        700        710        720 
KIWCVIPKQD PLVLYMYGAP QDVRAQATIP LLGYIVDDMP KSADLPHSFK LTQSKSVHSF 

       730        740        750        760 
AADSEELKQK WLKIILLAVT GETPDGPSEH LDTLDNLPGP KEKSEC 

« Hide

References

[1]"Frabin, a novel FGD1-related actin filament-binding protein capable of changing cell shape and activating c-Jun N-terminal kinase."
Obaishi H., Nakanishi H., Mandai K., Satoh K., Satoh A., Takahashi K., Miyahara M., Nishioka H., Takaishi K., Takai Y.
J. Biol. Chem. 273:18697-18700(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 41-65; 236-252; 276-291; 365-396; 464-475; 478-493; 496-503; 510-551; 702-710 AND 716-428, HOMOOLIGOMERIZATION, FUNCTION.
Tissue: Brain.
[2]"Association of frabin with the actin cytoskeleton is essential for microspike formation through activation of Cdc42 small G protein."
Umikawa M., Obaishi H., Nakanishi H., Satoh-Horikawa K., Takahashi K., Hotta I., Matsuura Y., Takai Y.
J. Biol. Chem. 274:25197-25200(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ACTIN FIBER-BINDING DOMAIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF038388 mRNA. Translation: AAC27698.1.
RefSeqNP_640356.1. NM_139263.1.
UniGeneRn.29988.

3D structure databases

ProteinModelPortalO88387.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000002491.

Proteomic databases

PRIDEO88387.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID246174.
KEGGrno:246174.

Organism-specific databases

CTD121512.
RGD708357. Fgd4.

Phylogenomic databases

eggNOGCOG5422.
HOGENOMHOG000220866.
HOVERGENHBG007506.
InParanoidO88387.
KOK05723.
PhylomeDBO88387.

Gene expression databases

GenevestigatorO88387.

Family and domain databases

Gene3D1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProIPR000219. DH-domain.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
PROSITEPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623462.
PROO88387.

Entry information

Entry nameFGD4_RAT
AccessionPrimary (citable) accession number: O88387
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families