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O88387

- FGD4_RAT

UniProt

O88387 - FGD4_RAT

Protein

FYVE, RhoGEF and PH domain-containing protein 4

Gene

Fgd4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri559 – 61961FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. actin binding Source: MGI
    2. actin filament binding Source: RGD
    3. metal ion binding Source: UniProtKB-KW
    4. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. activation of JUN kinase activity Source: MGI
    2. regulation of cell shape Source: RGD

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Actin-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FYVE, RhoGEF and PH domain-containing protein 4
    Alternative name(s):
    Actin filament-binding protein frabin
    FGD1-related F-actin-binding protein
    Gene namesi
    Name:Fgd4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi708357. Fgd4.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication. Cell projectionfilopodium 1 Publication
    Note: Concentrated in filopodia and poorly detected at lamellipodia. Binds along the sides of actin fibers.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. cytoskeleton Source: UniProtKB-SubCell
    3. filopodium Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 766766FYVE, RhoGEF and PH domain-containing protein 4PRO_0000080949Add
    BLAST

    Proteomic databases

    PRIDEiO88387.

    Expressioni

    Tissue specificityi

    Detected in brain, lung, liver, skeletal muscle, kidney, testis and cultured hippocampal neurons.1 Publication

    Gene expression databases

    GenevestigatoriO88387.

    Interactioni

    Subunit structurei

    Homooligomer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000002491.

    Structurei

    3D structure databases

    ProteinModelPortaliO88387.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini206 – 393188DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini422 – 521100PH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini643 – 74098PH 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 150150Actin filament-bindingAdd
    BLAST

    Domaini

    The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain.

    Sequence similaritiesi

    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
    Contains 2 PH domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri559 – 61961FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5422.
    HOGENOMiHOG000220866.
    HOVERGENiHBG007506.
    InParanoidiO88387.
    KOiK05723.
    PhylomeDBiO88387.

    Family and domain databases

    Gene3Di1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF01363. FYVE. 1 hit.
    PF00169. PH. 2 hits.
    PF00621. RhoGEF. 1 hit.
    [Graphical view]
    SMARTiSM00064. FYVE. 1 hit.
    SM00233. PH. 2 hits.
    SM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    PROSITEiPS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88387-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEESNPAPTS CASKGKHSKV SDLISHFEGG SVLSSYTDVQ KDSTMNLNIP    50
    QTPRQHGLTS TTPQKLPSHK SPQKQEKDSD QNQGQHGCLA NGVAAAQSQM 100
    ECETEKEAAL SPETDTQTAA ASPDAHVLNG VRNETTTDSA SSVTNSHDEN 150
    ACDSSCRTQG TDLGLPSKEG EPVIEAELQE RENGLSTEGL NPLDQHHEVK 200
    ETNEQKLHKI ATELLLTERA YVSRLNLLDQ VFYCKLLEEA NRGSFPAEMV 250
    NKIFSNISSI NAFHSKFLLP ELEKRMQEWE TTPRIGDILQ KLAPFLKMYG 300
    EYVKGFDNAV ELVKNMTERV PQFKSVTEEI QKQKICGSLT LQHHMLEPIQ 350
    RIPRYEMLLK DYLKKLSPDA PDWNDAKKSL EIISTAASHS NSAIRKMENL 400
    KKLLEIYEML GEEEDIVNPS NELIKEGQIL KLAARNTSAQ ERYLFLFNNM 450
    LLYCVPRFSL VGSKFTVRTR VGIDGMKIVE THNEEYPHTF QVSGKERTLE 500
    LQASSEQDKE EWIKALQESI DAFHQRHETF RNAIAKENDI PLEVSTAELG 550
    KRAPRWIRDN EVTMCMKCKE SFNALTRRRH HCRACGHVVC WKCSDYKAQL 600
    EYDGGRLNKV CKDCYQIMSG FAESEEKKRR GILEIESAEV SGNSEVCSFL 650
    QYMEKSKPWQ KIWCVIPKQD PLVLYMYGAP QDVRAQATIP LLGYIVDDMP 700
    KSADLPHSFK LTQSKSVHSF AADSEELKQK WLKIILLAVT GETPDGPSEH 750
    LDTLDNLPGP KEKSEC 766
    Length:766
    Mass (Da):86,454
    Last modified:November 1, 1998 - v1
    Checksum:iE34831DC0B0B334C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF038388 mRNA. Translation: AAC27698.1.
    RefSeqiNP_640356.1. NM_139263.1.
    UniGeneiRn.29988.

    Genome annotation databases

    GeneIDi246174.
    KEGGirno:246174.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF038388 mRNA. Translation: AAC27698.1 .
    RefSeqi NP_640356.1. NM_139263.1.
    UniGenei Rn.29988.

    3D structure databases

    ProteinModelPortali O88387.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000002491.

    Proteomic databases

    PRIDEi O88387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 246174.
    KEGGi rno:246174.

    Organism-specific databases

    CTDi 121512.
    RGDi 708357. Fgd4.

    Phylogenomic databases

    eggNOGi COG5422.
    HOGENOMi HOG000220866.
    HOVERGENi HBG007506.
    InParanoidi O88387.
    KOi K05723.
    PhylomeDBi O88387.

    Miscellaneous databases

    NextBioi 623462.
    PROi O88387.

    Gene expression databases

    Genevestigatori O88387.

    Family and domain databases

    Gene3Di 1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF01363. FYVE. 1 hit.
    PF00169. PH. 2 hits.
    PF00621. RhoGEF. 1 hit.
    [Graphical view ]
    SMARTi SM00064. FYVE. 1 hit.
    SM00233. PH. 2 hits.
    SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    PROSITEi PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Frabin, a novel FGD1-related actin filament-binding protein capable of changing cell shape and activating c-Jun N-terminal kinase."
      Obaishi H., Nakanishi H., Mandai K., Satoh K., Satoh A., Takahashi K., Miyahara M., Nishioka H., Takaishi K., Takai Y.
      J. Biol. Chem. 273:18697-18700(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 41-65; 236-252; 276-291; 365-396; 464-475; 478-493; 496-503; 510-551; 702-710 AND 716-428, HOMOOLIGOMERIZATION, FUNCTION.
      Tissue: Brain.
    2. "Association of frabin with the actin cytoskeleton is essential for microspike formation through activation of Cdc42 small G protein."
      Umikawa M., Obaishi H., Nakanishi H., Satoh-Horikawa K., Takahashi K., Hotta I., Matsuura Y., Takai Y.
      J. Biol. Chem. 274:25197-25200(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ACTIN FIBER-BINDING DOMAIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiFGD4_RAT
    AccessioniPrimary (citable) accession number: O88387
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3