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O88382

- MAGI2_RAT

UniProt

O88382 - MAGI2_RAT

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Protein

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2

Gene

Magi2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Seems to act as scaffold molecule at synaptic junctions by assembling neurotransmitter receptors and cell adhesion proteins. May play a role in regulating activin-mediated signaling in neuronal cells. Enhances the ability of PTEN to suppress AKT1 activation By similarity. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth.By similarity2 Publications

GO - Molecular functioni

  1. beta-1 adrenergic receptor binding Source: UniProtKB
  2. PDZ domain binding Source: RGD
  3. phosphatase binding Source: UniProtKB
  4. protein complex binding Source: RGD
  5. receptor signaling complex scaffold activity Source: UniProtKB
  6. signal transducer activity Source: UniProtKB
  7. SMAD binding Source: UniProtKB
  8. type II activin receptor binding Source: UniProtKB

GO - Biological processi

  1. cellular response to nerve growth factor stimulus Source: UniProtKB
  2. cytoplasmic transport Source: UniProtKB
  3. glomerular filtration Source: UniProtKB
  4. glomerular visceral epithelial cell development Source: UniProtKB
  5. mitotic cell cycle arrest Source: UniProtKB
  6. negative regulation of activin receptor signaling pathway Source: UniProtKB
  7. negative regulation of cell migration Source: UniProtKB
  8. negative regulation of cell proliferation Source: UniProtKB
  9. negative regulation of protein kinase B signaling Source: UniProtKB
  10. nerve growth factor signaling pathway Source: UniProtKB
  11. positive regulation of neuron projection development Source: UniProtKB
  12. positive regulation of phosphoprotein phosphatase activity Source: UniProtKB
  13. positive regulation of receptor internalization Source: UniProtKB
  14. protein heterooligomerization Source: RGD
  15. receptor clustering Source: RGD
  16. SMAD protein signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
Alternative name(s):
Atrophin-1-interacting protein 1
Short name:
AIP-1
Membrane-associated guanylate kinase inverted 2
Short name:
MAGI-2
Synaptic-scaffolding molecule
Short name:
S-SCAM
Gene namesi
Name:Magi2
Synonyms:Acvrinp1, Aip1, Sscam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621855. Magi2.

Subcellular locationi

Cytoplasm. Late endosome. Cell junctionsynapsesynaptosome. Cell membrane; Peripheral membrane protein
Note: Membrane-associated in synaptosomes. Localized diffusely in the cytoplasm before nerve growth factor (NGF) stimulation. Recruted to late endosomes after NGF stimulation.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: UniProtKB
  3. late endosome Source: UniProtKB
  4. nucleus Source: UniProtKB
  5. perinuclear region of cytoplasm Source: UniProtKB
  6. plasma membrane Source: UniProtKB
  7. postsynaptic density Source: UniProtKB
  8. protein complex Source: UniProtKB
  9. slit diaphragm Source: UniProtKB
  10. synapse Source: UniProtKB
  11. tight junction Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Endosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12771277Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2PRO_0000094588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei362 – 3621PhosphotyrosineBy similarity
Modified residuei827 – 8271PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88382.
PRIDEiO88382.

PTM databases

PhosphoSiteiO88382.

Expressioni

Tissue specificityi

Expressed in the foot process layer of podocytes of the kidney glomeruli but not in tubules (at protein level). Expressed in the brain.2 Publications

Developmental stagei

Expressed during the late capillary loop stage of glomerulogenesis. First detected in junctional complexes in podocytes after their migration to the base of cells. Up-regulated upon foot process differentiation.1 Publication

Gene expression databases

GenevestigatoriO88382.

Interactioni

Subunit structurei

Interacts (via its WW domains) with DRPLA By similarity. Interacts with CTNNB1, ACVR2A, SMAD2 and SMAD3 By similarity. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3 By similarity. May interact with HTR2A and IGSF9 By similarity. Interacts with HTR4 By similarity. Interacts (via guanylate kinase domain) with DLGAP1. Interacts (via PDZ domains) with GRIN2A, GRID2 and NLGN1. Interacts with CTNND2 and MAGUIN-1. Interacts (via its second PDZ domain) with PTEN (via unphosphorylated C-terminus); this interaction diminishes the degradation rate of PTEN. Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a NGF-dependent manner. Interacts with RAPGEF2; the interaction occurs before or after nerve growth factor (NGF) stimulation. Isoform 1 interacts (via PDZ domain) with KIDINS220 isoform 2 (via C-terminal domain). Interacts with DDN. Identified in a complex with ACTN4, CASK, IQGAP1, NPHS1, SPTAN1 and SPTBN1.By similarity10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DDNO948503EBI-696179,EBI-5240523From a different organism.
Dlgap1P978363EBI-696179,EBI-80901
GraspQ8R4T55EBI-696179,EBI-7361884
PTENP604843EBI-696179,EBI-696162From a different organism.

Protein-protein interaction databases

BioGridi250241. 7 interactions.
IntActiO88382. 26 interactions.
MINTiMINT-87246.
STRINGi10116.ENSRNOP00000036634.

Structurei

3D structure databases

ProteinModelPortaliO88382.
SMRiO88382. Positions 303-340, 347-395, 412-522, 594-682, 769-865, 916-1021, 1135-1230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 10185PDZPROSITE-ProRule annotationAdd
BLAST
Domaini109 – 283175Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST
Domaini302 – 33534WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini348 – 38134WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini426 – 51085PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini605 – 68379PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini778 – 86083PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini920 – 101091PDZ 4PROSITE-ProRule annotationAdd
BLAST
Domaini1141 – 122383PDZ 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni302 – 38180Interaction with DDNBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 5 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5021.
HOGENOMiHOG000113463.
HOVERGENiHBG007091.
InParanoidiO88382.
KOiK05629.
PhylomeDBiO88382.

Family and domain databases

Gene3Di2.30.42.10. 6 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 6 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88382-1) [UniParc]FASTAAdd to Basket

Also known as: alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKSLKKKSH WTSKVHESVI GRNPEGQLGF ELKGGAENGQ FPYLGEVKPG
60 70 80 90 100
KVAYESGSKL VSEELLLEVN ETPVAGLTIR DVLAVIKHCK DPLRLKCVKQ
110 120 130 140 150
GGIVDKDLRH YLNLRFQKGS VDHELQQIIR DNLYLRTVPC TTRPHKEGEV
160 170 180 190 200
PGVDYIFITV EDFMELEKSG ALLESGTYED NYYGTPKPPA EPAPLLLNVT
210 220 230 240 250
DQILPGATPS AEGKRKRNKS VTNMEKASIE PPEEEEEERP VVNGNGVVIT
260 270 280 290 300
PESSEHEDKS AGASGETPSQ PYPAPVYSQP EELKDQMDDT KSTKPEENED
310 320 330 340 350
SDPLPDNWEM AYTEKGEVYF IDHNTKTTSW LDPRLAKKAK PAEECKENEL
360 370 380 390 400
PYGWEKIDDP IYGTYYVDHI NRRTQFENPV LEAKRKLQQH NMPHTELGTK
410 420 430 440 450
PLQAPGFREK PLFTRDASQL KGTFLSTTLK KSNMGFGFTI IGGDEPDEFL
460 470 480 490 500
QVKSVIPDGP AAQDGKMETG DVIVYINEVC VLGHTHADVV KLFQSVPIGQ
510 520 530 540 550
SVNLVLCRGY PLPFDPEDPA NSMVPPLAIM ERPPPVMVNG RHNYETYLEY
560 570 580 590 600
ISRTSQSVPD ITDRPPHSLH SMPADGQLDG TYPPPVHDDN VSVASSGATQ
610 620 630 640 650
AELMTLTIVK GAKGFGFTIA DSPTGQRVKQ ILDIQGCPGL CEGDLIVEIN
660 670 680 690 700
QQNVQNLSHT EVVDILKDCP VGSETSLIIH RGGFFSPWKT PKPMVDRWEN
710 720 730 740 750
QGSPQTSLSA PAVPQSLPFP PALHRSSFPD STEAFDPRKP DPYELYEKSR
760 770 780 790 800
AIYESRQQVP PRTSFRMDSS GPDYKELDVH LRRMESGFGF RILGGDEPGQ
810 820 830 840 850
PILIGAVIAM GSADRDGRLH PGDELVYVDG IPVAGKTHRY VIDLMHHAAR
860 870 880 890 900
NGQVNLTVRR KVLCGGEPCP ENGRSPGSVS THHSSPRSDY ATYANSNHAA
910 920 930 940 950
PSNNASPPEG FASHSLQTSD VIIHRKENEG FGFVIISSLN RPESGATITV
960 970 980 990 1000
PHKIGRIIDG SPADRCAKLK VGDRILAVNG QSIINMPHAD IVKLIKDAGL
1010 1020 1030 1040 1050
SVTLRIIPQE ELNNPTSAPS SEKQSPMAQQ HSPLAQQHSP LAQPSPATPN
1060 1070 1080 1090 1100
SPVAQPAPPQ PLQLQGHENS YRSEVKARQD VKPDIRQPPF TDYRQPPLDY
1110 1120 1130 1140 1150
RQPPGGDYSQ PSPLDYRQHS PDTRQYPLSD YRQPQDFDYF TVDMEKGAKG
1160 1170 1180 1190 1200
FGFSIRGGRE YKMDLYVLRL AEDGPAIRNG RMRVGDQIIE INGESTRDMT
1210 1220 1230 1240 1250
HARAIELIKS GGRRVRLLLK RGTGQVPEYG MVPSSLSMCM KSDKHGSPYF
1260 1270
YLLGHPKDTT NPTPGALPLP PPQACRK
Length:1,277
Mass (Da):141,072
Last modified:November 1, 1998 - v1
Checksum:iE1A435FF35549DF9
GO
Isoform 2 (identifier: O88382-2) [UniParc]FASTAAdd to Basket

Also known as: beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.

Show »
Length:1,114
Mass (Da):122,766
Checksum:i7B1BA3E0049DBD30
GO
Isoform 3 (identifier: O88382-3) [UniParc]FASTAAdd to Basket

Also known as: gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-223: Missing.

Show »
Length:1,054
Mass (Da):116,325
Checksum:i718C43F950E5365E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti645 – 6451L → F in AAD31015. (PubMed:10644767)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 223223Missing in isoform 3. CuratedVSP_008439Add
BLAST
Alternative sequencei1 – 163163Missing in isoform 2. 1 PublicationVSP_008438Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF034863 mRNA. Translation: AAC31124.1.
AF130819 mRNA. Translation: AAD31015.1.
PIRiT14152.
RefSeqiNP_446073.1. NM_053621.1. [O88382-1]
UniGeneiRn.205058.

Genome annotation databases

GeneIDi113970.
KEGGirno:113970.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF034863 mRNA. Translation: AAC31124.1 .
AF130819 mRNA. Translation: AAD31015.1 .
PIRi T14152.
RefSeqi NP_446073.1. NM_053621.1. [O88382-1 ]
UniGenei Rn.205058.

3D structure databases

ProteinModelPortali O88382.
SMRi O88382. Positions 303-340, 347-395, 412-522, 594-682, 769-865, 916-1021, 1135-1230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250241. 7 interactions.
IntActi O88382. 26 interactions.
MINTi MINT-87246.
STRINGi 10116.ENSRNOP00000036634.

PTM databases

PhosphoSitei O88382.

Proteomic databases

PaxDbi O88382.
PRIDEi O88382.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 113970.
KEGGi rno:113970.

Organism-specific databases

CTDi 9863.
RGDi 621855. Magi2.

Phylogenomic databases

eggNOGi COG5021.
HOGENOMi HOG000113463.
HOVERGENi HBG007091.
InParanoidi O88382.
KOi K05629.
PhylomeDBi O88382.

Miscellaneous databases

NextBioi 618109.
PROi O88382.

Gene expression databases

Genevestigatori O88382.

Family and domain databases

Gene3Di 2.30.42.10. 6 hits.
InterProi IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 6 hits.
PF00397. WW. 2 hits.
[Graphical view ]
SMARTi SM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel multiple PDZ domain-containing molecule interacting with N-methyl-d-aspartate receptors and neuronal cell adhesion proteins."
    Hirao K., Hata Y., Ide N., Takeuchi M., Irie M., Yao I., Deguchi M., Toyoda A., Suedhof T.C., Takai Y.
    J. Biol. Chem. 273:21105-21110(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH DLGAP1; NLGN1 AND GRIN2A.
  2. "Three isoforms of synaptic scaffolding molecule and their characterization: multimerization between the isoforms and their interaction with N-methyl-D-aspartate receptors and SAP90/PSD-95-associated protein."
    Hirao K., Hata Y., Yao I., Deguchi M., Kawabe H., Mizoguchi A., Takai Y.
    J. Biol. Chem. 275:2966-2972(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING (ISOFORM 3).
  3. "Interaction of S-SCAM with neural plakophilin-related Armadillo-repeat protein/delta-catenin."
    Ide N., Hata Y., Deguchi M., Hirao K., Yao I., Takai Y.
    Biochem. Biophys. Res. Commun. 256:456-461(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CTNND2.
  4. "nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM)."
    Ohtsuka T., Hata Y., Ide N., Yasuda T., Inoue E., Inoue T., Mizoguchi A., Takai Y.
    Biochem. Biophys. Res. Commun. 265:38-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAPGEF2.
  5. "MAGUIN, a novel neuronal membrane-associated guanylate kinase-interacting protein."
    Yao I., Hata Y., Ide N., Hirao K., Deguchi M., Nishioka H., Mizoguchi A., Takai Y.
    J. Biol. Chem. 274:11889-11896(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAGUIN-1.
  6. "PKC regulates the delta2 glutamate receptor interaction with S-SCAM/MAGI-2 protein."
    Yap C.C., Muto Y., Kishida H., Hashikawa T., Yano R.
    Biochem. Biophys. Res. Commun. 301:1122-1128(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRID2.
  7. "Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
    Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
    J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTEN.
  8. "Cell junction-associated proteins IQGAP1, MAGI-2, CASK, spectrins, and alpha-actinin are components of the nephrin multiprotein complex."
    Lehtonen S., Ryan J.J., Kudlicka K., Iino N., Zhou H., Farquhar M.G.
    Proc. Natl. Acad. Sci. U.S.A. 102:9814-9819(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH ACTN4; CASK; IQGAP1; NPHS1; SPTAN1 AND SPTBN1, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
    Tissue: Renal glomerulus.
  9. "CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin."
    Kawata A., Iida J., Ikeda M., Sato Y., Mori H., Kansaku A., Sumita K., Fujiwara N., Rokukawa C., Hamano M., Hirabayashi S., Hata Y.
    J. Biochem. 139:931-939(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DDN.
  10. "Postsynaptic recruitment of Dendrin depends on both dendritic mRNA transport and synaptic anchoring."
    Kremerskothen J., Kindler S., Finger I., Veltel S., Barnekow A.
    J. Neurochem. 96:1659-1666(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DDN.
  11. "Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth."
    Hisata S., Sakisaka T., Baba T., Yamada T., Aoki K., Matsuda M., Takai Y.
    J. Cell Biol. 178:843-860(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH KIDINS220; RAPGEF2 AND NTRK1, INTERACTION WITH RAPGEF2 AND KIDINS220, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMAGI2_RAT
AccessioniPrimary (citable) accession number: O88382
Secondary accession number(s): Q9R271
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3