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Protein

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta

Gene

Pip4k2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: Ensembl
  2. 1-phosphatidylinositol-5-phosphate 4-kinase activity Source: UniProtKB-EC
  3. ATP binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198600. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC:2.7.1.149)
Alternative name(s):
1-phosphatidylinositol 5-phosphate 4-kinase 2-beta
Diphosphoinositide kinase 2-beta
Phosphatidylinositol 5-phosphate 4-kinase type II beta
Short name:
PI(5)P 4-kinase type II beta
Short name:
PIP4KII-beta
Phosphatidylinositol-phosphate kinase IIgamma
Short name:
PIPKIIgamma
PtdIns(5)P-4-kinase isoform 2-beta
Gene namesi
Name:Pip4k2b
Synonyms:Pip5k2b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi621710. Pip4k2b.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Cytoplasm By similarity
Note: Associated with the plasma membrane and the endoplasmic reticulum.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 416415Phosphatidylinositol 5-phosphate 4-kinase type-2 betaPRO_0000185472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei150 – 1501N6-acetyllysineBy similarity
Modified residuei326 – 3261PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serine residues. Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88377.
PRIDEiO88377.

Expressioni

Gene expression databases

GenevestigatoriO88377.

Interactioni

Subunit structurei

Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex (By similarity).By similarity

Protein-protein interaction databases

IntActiO88377. 1 interaction.
STRINGi10116.ENSRNOP00000017989.

Structurei

3D structure databases

ProteinModelPortaliO88377.
SMRiO88377. Positions 34-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 415378PIPKPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi303 – 3086Poly-Glu

Sequence similaritiesi

Contains 1 PIPK domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5253.
GeneTreeiENSGT00760000119184.
HOGENOMiHOG000007832.
HOVERGENiHBG000072.
InParanoidiO88377.
KOiK00920.
OMAiECENDGL.
OrthoDBiEOG708VZZ.
PhylomeDBiO88377.
TreeFamiTF354315.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNCTSTTA VAVAPLSASK TKTKKKHFVC QKVKLFRASE PILSVLMWGV
60 70 80 90 100
NHTINELSNV PVPVMLMPDD FKAYSKIKVD NHLFNKENLP SRFKFKEYCP
110 120 130 140 150
MVFRNLRERF GIDDQDYQNS VTRSAPINSD SQGRCGTRFL TTYDRRFVIK
160 170 180 190 200
TVSSEDVAEM HNILKKYHQF IVECHGNTLL PQFLGMYRLT VDGVETYMVV
210 220 230 240 250
TRNVFSHRLT VHRKYDLKGS TVAREASDKE KAKDLPTFKD NDFLNEGQKL
260 270 280 290 300
RVGEESKKNF LEKLKRDVEF LAQLKIMDYS LLVGIHDVDR AEQEETEVED
310 320 330 340 350
RAEEEECEND GVGGGLLCSY GTPPDSPGNL LSFPRFFGPG EFDPSVDVYA
360 370 380 390 400
MKSHESAPKK EVYFMAIIDI LTPYDAKKKA AHAAKTVKHG AGAEISTVNP
410
EQYSKRFNEF MSNILT
Length:416
Mass (Da):47,264
Last modified:November 1, 1998 - v1
Checksum:i9481AC50CBB6C196
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033355 mRNA. Translation: AAC40203.1.
RefSeqiNP_446002.1. NM_053550.1.
UniGeneiRn.30025.

Genome annotation databases

EnsembliENSRNOT00000017989; ENSRNOP00000017989; ENSRNOG00000013030.
GeneIDi89812.
KEGGirno:89812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033355 mRNA. Translation: AAC40203.1.
RefSeqiNP_446002.1. NM_053550.1.
UniGeneiRn.30025.

3D structure databases

ProteinModelPortaliO88377.
SMRiO88377. Positions 34-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88377. 1 interaction.
STRINGi10116.ENSRNOP00000017989.

Proteomic databases

PaxDbiO88377.
PRIDEiO88377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017989; ENSRNOP00000017989; ENSRNOG00000013030.
GeneIDi89812.
KEGGirno:89812.

Organism-specific databases

CTDi8396.
RGDi621710. Pip4k2b.

Phylogenomic databases

eggNOGiCOG5253.
GeneTreeiENSGT00760000119184.
HOGENOMiHOG000007832.
HOVERGENiHBG000072.
InParanoidiO88377.
KOiK00920.
OMAiECENDGL.
OrthoDBiEOG708VZZ.
PhylomeDBiO88377.
TreeFamiTF354315.

Enzyme and pathway databases

ReactomeiREACT_198600. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

NextBioi617686.
PROiO88377.

Gene expression databases

GenevestigatoriO88377.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel phosphatidylinositol-5-phosphate 4-kinase (phosphatidylinositol-phosphate kinase IIgamma) is phosphorylated in the endoplasmic reticulum in response to mitogenic signals."
    Itoh T., Ijuin T., Takenawa T.
    J. Biol. Chem. 273:20292-20299(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
    Tissue: Brain.

Entry informationi

Entry nameiPI42B_RAT
AccessioniPrimary (citable) accession number: O88377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: November 1, 1998
Last modified: March 4, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.