Reviewed, UniProtKB/Swiss-Prot O88345 (PDK4_SPETR)

Names and origin

Protein names

Recommended name:
    [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
    EC=2.7.11.2
Alternative name(s):
    Pyruvate dehydrogenase kinase isoform 4

Gene names

Name:

PDK4

Organism

Spermophilus tridecemlineatus (Thirteen-lined ground squirrel)

Taxonomic identifier

43179 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Sciuridae › Xerinae › Marmotini › Spermophilus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subcellular location	Mitochondrion matrix.
Induction	Up-regulated during hibernation.
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism Hibernation
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW hibernation Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate dehydrogenase (acetyl-transferring) kinase activity Inferred from electronic annotation. Source: EC two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 412		[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial		PRO_0000023448
Regions
	Domain	138 – 368	231	Histidine kinase
Amino acid modifications
	Modified residue	127	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O88345-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 4F51628DCBC84DBB	FASTA	412	46,837
	10 20 30 40 50 60 MKAARFAMHS ARSLSSVGLV PREVELFSRY SPSPLSMKQL LDFGSDNACE RTSFSFLRQE 70 80 90 100 110 120 LPVRLANILK EIDVLPDRLT NTSSVQLVKS WYIQSLMELV EFHEKSPEDQ KNLSDFVDTL 130 140 150 160 170 180 IKVRNRHHNV VPTMAQGILE YKDTCTVDPV TNQSLQYFLD RFYMNRISTR MLMNQHILIF 190 200 210 220 230 240 SDSQTGNPSH IGSIDPKCDV VAVIQDAFES SKMLCDQYYL TSPELKLTQV NGKFPGQPIH 250 260 270 280 290 300 IVYVPSHLHH MLFELFKNAM RATVERQESW PSLTPVEVIV VLGKEDLTIK ISDRGGGVPL 310 320 330 340 350 360 RITDRLFSYM YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL YSLSGYGTDA 370 380 390 400 410 IIYLKALSSE SVEKLPVFNK SAFKHYQMSS EADDWCIPSR EPRNLSKEKM AM

References

[1]	"Low-temperature carbon utilization is regulated by novel gene activity in the heart of a hibernating mammal." Andrews M.T., Squire T.L., Bowen C.M., Rollins M.B. Proc. Natl. Acad. Sci. U.S.A. 95:8392-8397(1998) [PubMed: 9653197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF020845 mRNA. Translation: AAC40161.1.
3D structure databases
HSSP	HSSP built from PDB template 1JM6 based on UniProtKB Q64536.
ModBase	Search...
Phylogenomic databases
HOVERGEN	O88345.
Enzyme and pathway databases
BRENDA	2.7.11.2. 277892.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR018955. BCDHK/PDK_mit. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDK4_SPETR

Accession

Primary (citable) accession number: O88345

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)