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Protein

ATP-sensitive inward rectifier potassium channel 1

Gene

Kcnj1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium (By similarity).By similarity

Enzyme regulationi

Inhibited by WNK3.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei152 – 1521Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi204 – 2118ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cardiovascular system development Source: MGI
  • kidney development Source: MGI
  • post-embryonic development Source: MGI
  • regulation of G-protein activated inward rectifier potassium channel activity Source: MGI
  • renal sodium ion absorption Source: MGI
  • tissue homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296067. Potassium transport channels.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 1
Alternative name(s):
ATP-regulated potassium channel ROM-K
Inward rectifier K(+) channel Kir1.1
Potassium channel, inwardly rectifying subfamily J member 1
Gene namesi
Name:Kcnj1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1927248. Kcnj1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5858CytoplasmicBy similarityAdd
BLAST
Transmembranei59 – 8325Helical; Name=M1By similarityAdd
BLAST
Topological domaini84 – 10825ExtracellularBy similarityAdd
BLAST
Intramembranei109 – 12012Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei121 – 1277Pore-formingBy similarity
Topological domaini128 – 1369ExtracellularBy similarity
Transmembranei137 – 15822Helical; Name=M2By similarityAdd
BLAST
Topological domaini159 – 372214CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372ATP-sensitive inward rectifier potassium channel 1PRO_0000154918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251Phosphoserine; by SGK1By similarity
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylation at Ser-25 by SGK1 is necessary for its expression at the cell membrane.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88335.
PRIDEiO88335.

PTM databases

iPTMnetiO88335.
PhosphoSiteiO88335.

Expressioni

Gene expression databases

BgeeiO88335.
ExpressionAtlasiO88335. baseline and differential.
GenevisibleiO88335. MM.

Interactioni

Subunit structurei

Interacts with SGK1 and SLC9A3R2/NHERF2.By similarity

Protein-protein interaction databases

IntActiO88335. 1 interaction.
STRINGi10090.ENSMUSP00000131625.

Structurei

3D structure databases

ProteinModelPortaliO88335.
SMRiO88335. Positions 20-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 1276Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237326.
HOVERGENiHBG006178.
InParanoidiO88335.
KOiK04995.
PhylomeDBiO88335.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003268. K_chnl_inward-rec_Kir1.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF6. PTHR11767:SF6. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01321. KIR11CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.

Isoform 1 (identifier: O88335-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFKHLRRWFV THIFGRSRQR ARLVSKDGRC NIEFGNVDAQ SRFIFFVDIW
60 70 80 90 100
TTVLDLKWRY KMTVFITAFL GSWFLFGLLW YVVAYVHKDL PEFYPPDNRT
110 120 130 140 150
PCVENINGMT SAFLFSLETQ VTIGYGFRFV TEQCATAIFL LIFQSILGVI
160 170 180 190 200
INSFMCGAIL AKISRPKKRA KTITFSKNAV ISKRGGKLCL LIRVANLRKS
210 220 230 240 250
LLIGSHIYGK LLKTTITPEG ETIILDQTNI NFVVDAGNEN LFFISPLTIY
260 270 280 290 300
HIIDHNSPFF HMAAETLSQQ DFELVVFLDG TVESTSATCQ VRTSYIPEEV
310 320 330 340 350
LWGYRFVPIV SKTKEGKYRV DFHNFGKTVE VETPHCAMCL YNEKDARARM
360 370
KRGYDNPNFV LSEVDETDDT QM
Length:372
Mass (Da):42,776
Last modified:November 1, 1998 - v1
Checksum:iEA95D896622232D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012834 mRNA. Translation: AAC24973.1.
BC020525 mRNA. Translation: AAH20525.1.
CCDSiCCDS22953.1. [O88335-1]
RefSeqiNP_062633.1. NM_019659.3. [O88335-1]
UniGeneiMm.390168.

Genome annotation databases

EnsembliENSMUST00000047334; ENSMUSP00000046793; ENSMUSG00000041248. [O88335-1]
GeneIDi56379.
KEGGimmu:56379.
UCSCiuc009orx.2. mouse. [O88335-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012834 mRNA. Translation: AAC24973.1.
BC020525 mRNA. Translation: AAH20525.1.
CCDSiCCDS22953.1. [O88335-1]
RefSeqiNP_062633.1. NM_019659.3. [O88335-1]
UniGeneiMm.390168.

3D structure databases

ProteinModelPortaliO88335.
SMRiO88335. Positions 20-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88335. 1 interaction.
STRINGi10090.ENSMUSP00000131625.

PTM databases

iPTMnetiO88335.
PhosphoSiteiO88335.

Proteomic databases

PaxDbiO88335.
PRIDEiO88335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047334; ENSMUSP00000046793; ENSMUSG00000041248. [O88335-1]
GeneIDi56379.
KEGGimmu:56379.
UCSCiuc009orx.2. mouse. [O88335-1]

Organism-specific databases

CTDi3758.
MGIiMGI:1927248. Kcnj1.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237326.
HOVERGENiHBG006178.
InParanoidiO88335.
KOiK04995.
PhylomeDBiO88335.

Enzyme and pathway databases

ReactomeiR-MMU-1296067. Potassium transport channels.

Miscellaneous databases

PROiO88335.
SOURCEiSearch...

Gene expression databases

BgeeiO88335.
ExpressionAtlasiO88335. baseline and differential.
GenevisibleiO88335. MM.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003268. K_chnl_inward-rec_Kir1.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF6. PTHR11767:SF6. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01321. KIR11CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of alternatively spliced isoforms of mouse ROMK."
    Xu J.Z., Mount D.B., Hebert S.C.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.

Entry informationi

Entry nameiKCNJ1_MOUSE
AccessioniPrimary (citable) accession number: O88335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.