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Protein

ATP-sensitive inward rectifier potassium channel 1

Gene

Kcnj1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium (By similarity).By similarity

Enzyme regulationi

Inhibited by WNK3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei152Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 211ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • cardiovascular system development Source: MGI
  • cellular response to magnesium ion Source: MGI
  • kidney development Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • post-embryonic development Source: MGI
  • potassium ion import Source: GO_Central
  • potassium ion transport Source: MGI
  • regulation of G-protein activated inward rectifier potassium channel activity Source: MGI
  • renal sodium ion absorption Source: MGI
  • tissue homeostasis Source: MGI

Keywordsi

Molecular functionIon channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandATP-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296067 Potassium transport channels

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 1
Alternative name(s):
ATP-regulated potassium channel ROM-K
Inward rectifier K(+) channel Kir1.1
Potassium channel, inwardly rectifying subfamily J member 1
Gene namesi
Name:Kcnj1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1927248 Kcnj1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 58CytoplasmicBy similarityAdd BLAST58
Transmembranei59 – 83Helical; Name=M1By similarityAdd BLAST25
Topological domaini84 – 108ExtracellularBy similarityAdd BLAST25
Intramembranei109 – 120Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei121 – 127Pore-formingBy similarity7
Topological domaini128 – 136ExtracellularBy similarity9
Transmembranei137 – 158Helical; Name=M2By similarityAdd BLAST22
Topological domaini159 – 372CytoplasmicBy similarityAdd BLAST214

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549181 – 372ATP-sensitive inward rectifier potassium channel 1Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25Phosphoserine; by SGK1By similarity1
Glycosylationi98N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylation at Ser-25 by SGK1 is necessary for its expression at the cell membrane.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88335
PRIDEiO88335

PTM databases

iPTMnetiO88335
PhosphoSitePlusiO88335

Expressioni

Gene expression databases

BgeeiENSMUSG00000041248
ExpressionAtlasiO88335 baseline and differential
GenevisibleiO88335 MM

Interactioni

Subunit structurei

Interacts with SGK1 and SLC9A3R2/NHERF2.By similarity

Protein-protein interaction databases

BioGridi207940, 2 interactors
IntActiO88335, 3 interactors
STRINGi10090.ENSMUSP00000131625

Structurei

3D structure databases

ProteinModelPortaliO88335
SMRiO88335
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi122 – 127Selectivity filterBy similarity6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827 Eukaryota
ENOG410XQ62 LUCA
GeneTreeiENSGT00760000118842
HOGENOMiHOG000237326
HOVERGENiHBG006178
InParanoidiO88335
KOiK04995
PhylomeDBiO88335

Family and domain databases

Gene3Di2.60.40.1400, 2 hits
InterProiView protein in InterPro
IPR014756 Ig_E-set
IPR016449 K_chnl_inward-rec_Kir
IPR003268 K_chnl_inward-rec_Kir1.1
IPR013518 K_chnl_inward-rec_Kir_cyto
PANTHERiPTHR11767 PTHR11767, 1 hit
PTHR11767:SF6 PTHR11767:SF6, 1 hit
PfamiView protein in Pfam
PF01007 IRK, 1 hit
PIRSFiPIRSF005465 GIRK_kir, 1 hit
PRINTSiPR01321 KIR11CHANNEL
PR01320 KIRCHANNEL
SUPFAMiSSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: O88335-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFKHLRRWFV THIFGRSRQR ARLVSKDGRC NIEFGNVDAQ SRFIFFVDIW
60 70 80 90 100
TTVLDLKWRY KMTVFITAFL GSWFLFGLLW YVVAYVHKDL PEFYPPDNRT
110 120 130 140 150
PCVENINGMT SAFLFSLETQ VTIGYGFRFV TEQCATAIFL LIFQSILGVI
160 170 180 190 200
INSFMCGAIL AKISRPKKRA KTITFSKNAV ISKRGGKLCL LIRVANLRKS
210 220 230 240 250
LLIGSHIYGK LLKTTITPEG ETIILDQTNI NFVVDAGNEN LFFISPLTIY
260 270 280 290 300
HIIDHNSPFF HMAAETLSQQ DFELVVFLDG TVESTSATCQ VRTSYIPEEV
310 320 330 340 350
LWGYRFVPIV SKTKEGKYRV DFHNFGKTVE VETPHCAMCL YNEKDARARM
360 370
KRGYDNPNFV LSEVDETDDT QM
Length:372
Mass (Da):42,776
Last modified:November 1, 1998 - v1
Checksum:iEA95D896622232D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012834 mRNA Translation: AAC24973.1
BC020525 mRNA Translation: AAH20525.1
CCDSiCCDS22953.1 [O88335-1]
RefSeqiNP_062633.1, NM_019659.3 [O88335-1]
UniGeneiMm.390168

Genome annotation databases

EnsembliENSMUST00000047334; ENSMUSP00000046793; ENSMUSG00000041248 [O88335-1]
ENSMUST00000213393; ENSMUSP00000150540; ENSMUSG00000041248 [O88335-1]
GeneIDi56379
KEGGimmu:56379
UCSCiuc009orx.2 mouse [O88335-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKCNJ1_MOUSE
AccessioniPrimary (citable) accession number: O88335
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1998
Last modified: June 20, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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