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Protein

Nidogen-2

Gene

Nid2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.

GO - Molecular functioni

GO - Biological processi

  • cell-matrix adhesion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_286043. Laminin interactions.

Protein family/group databases

MEROPSiI63.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Nidogen-2
Short name:
NID-2
Alternative name(s):
Entactin-2
Gene namesi
Name:Nid2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1298229. Nid2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • cell surface Source: Ensembl
  • extracellular exosome Source: MGI
  • extracellular matrix Source: UniProtKB
  • extracellular region Source: Reactome
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030By similarityAdd
BLAST
Chaini31 – 14031373Nidogen-2PRO_0000007672Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi511 ↔ 524By similarity
Disulfide bondi518 ↔ 533By similarity
Disulfide bondi535 ↔ 546By similarity
Glycosylationi681 – 6811N-linked (GlcNAc...)1 Publication
Glycosylationi716 – 7161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi726 – 7261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi786 ↔ 799By similarity
Disulfide bondi793 ↔ 809By similarity
Disulfide bondi811 ↔ 822By similarity
Disulfide bondi828 ↔ 841By similarity
Disulfide bondi835 ↔ 850By similarity
Disulfide bondi852 ↔ 865By similarity
Disulfide bondi875 ↔ 890By similarity
Disulfide bondi882 ↔ 900By similarity
Disulfide bondi902 ↔ 913By similarity
Disulfide bondi919 ↔ 930By similarity
Disulfide bondi924 ↔ 939By similarity
Disulfide bondi941 ↔ 952By similarity
Disulfide bondi968 ↔ 991By similarity
Disulfide bondi1002 ↔ 1009By similarity
Disulfide bondi1011 ↔ 1033By similarity
Disulfide bondi1047 ↔ 1071By similarity
Disulfide bondi1082 ↔ 1089By similarity
Disulfide bondi1091 ↔ 1112By similarity
Glycosylationi1152 – 11521N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Highly N- and O-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO88322.
PaxDbiO88322.
PRIDEiO88322.

Expressioni

Gene expression databases

BgeeiO88322.
CleanExiMM_NID2.
GenevisibleiO88322. MM.

Interactioni

Subunit structurei

Interacts with LAMA2. Interacts with COL13A1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201771. 4 interactions.
STRINGi10090.ENSMUSP00000022340.

Structurei

3D structure databases

ProteinModelPortaliO88322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 274167NIDOPROSITE-ProRule annotationAdd
BLAST
Domaini507 – 54741EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini551 – 781231Nidogen G2 beta-barrelPROSITE-ProRule annotationAdd
BLAST
Domaini782 – 82342EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini824 – 86239EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini871 – 91444EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini915 – 95339EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini965 – 103369Thyroglobulin type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini1044 – 111269Thyroglobulin type-1 2PROSITE-ProRule annotationAdd
BLAST
Repeati1182 – 122544LDL-receptor class B 1Add
BLAST
Repeati1226 – 126843LDL-receptor class B 2Add
BLAST
Repeati1269 – 131345LDL-receptor class B 3Add
BLAST
Repeati1314 – 135542LDL-receptor class B 4Add
BLAST
Repeati1357 – 140145LDL-receptor class B 5Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi946 – 9483Cell attachment site

Sequence similaritiesi

Contains 5 EGF-like domains.PROSITE-ProRule annotation
Contains 5 LDL-receptor class B repeats.PROSITE-ProRule annotation
Contains 1 NIDO domain.PROSITE-ProRule annotation
Contains 1 nidogen G2 beta-barrel domain.PROSITE-ProRule annotation
Contains 2 thyroglobulin type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG282695.
GeneTreeiENSGT00760000118968.
HOGENOMiHOG000072712.
HOVERGENiHBG006498.
InParanoidiO88322.
KOiK06826.
OMAiARVGFCR.
OrthoDBiEOG7M0NQN.
TreeFamiTF320666.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.40.155.10. 2 hits.
4.10.800.10. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR006605. G2_nidogen/fibulin_G2F.
IPR009017. GFP.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR003886. Nidogen_extracell_dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07645. EGF_CA. 2 hits.
PF07474. G2F. 1 hit.
PF00058. Ldl_recept_b. 2 hits.
PF06119. NIDO. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00682. G2F. 1 hit.
SM00135. LY. 4 hits.
SM00539. NIDO. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF54511. SSF54511. 1 hit.
SSF57184. SSF57184. 2 hits.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 2 hits.
PS51120. LDLRB. 4 hits.
PS51220. NIDO. 1 hit.
PS50993. NIDOGEN_G2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRDPTAGWL TPPSPLSLLV MLLLLSRVGA LRPDELFPYG ESWGDQLLPE
60 70 80 90 100
GDDESSAAVK LAIPLRFYDA QFSSLYVGTN GIISTQDFPR ETQYVDDDFP
110 120 130 140 150
TDFPAIAPFL ADIDTSHSRG RILYREDTSG AVLSLAARYV RTGFPLSGSS
160 170 180 190 200
FTPTHAFLAT WEHVGAYEEV SRGAAPSGEL NTFQAVLASD ESDTYALFLY
210 220 230 240 250
PANGLQFFGT RPKESYNVQL QLPARVGFCR GEADDLKREA LYFSLTNTEQ
260 270 280 290 300
SVKNLYQLSN LGIPGVWAFH IGSRFALDNV RPATVGGDPS TARSSALEHP
310 320 330 340 350
FSHAAALESY TEDSFHYYDE NEEDVEYPPV EPGEAPEGHS RIDVSFNSKA
360 370 380 390 400
DPGLVDVGTS SPGSDRASPW PYPAPGNWPS YRETESASLD PQTKQGRPVG
410 420 430 440 450
EGEVLDFRDP AELLDQMGTR APAPPEADAA LLTPVNEDLG GRNTQSYPEA
460 470 480 490 500
GPVPSEPDVP VPPLEGEVLP HYPESGHVPP LRGGKYVIGL EDHVGSNDQV
510 520 530 540 550
FTYNGANLET CEHSHGRCSQ HAFCTDYTTG FCCHCQSRFY GNGKHCLPEG
560 570 580 590 600
APHRVNGKVS GRLRVGHIPV HFTDVDLHAY IVGNDGRAYT AISHVPQPAA
610 620 630 640 650
QALLPVLPIG GLFGWLFALE KPGSENGFSL TGATFVHDVE VTFHPGEERV
660 670 680 690 700
RITQTAEGLD PENYLSIKTN IEGQVPFIPA NFTAHITPYK EFYHYRDSVV
710 720 730 740 750
TSSSSRSFSL TSGSINQTWS YHIDQNITYQ ACRHAPRHLA IPATQQLTVD
760 770 780 790 800
RAFALYSEDE GVLRFAVTNQ IGPVEVDSAP VGVNPCYDGS HTCDTTARCH
810 820 830 840 850
PGTGVDYTCE CTPGFQGDGR SCVDVNECAT GFHRCGPNSV CVNLVGSYRC
860 870 880 890 900
ECRSGYEFAD DQHTCILIAP PPNPCLDGSH TCAPEGQARC IHHGGSSFSC
910 920 930 940 950
ACLPGFIGTG HQCSDVDECA ENRCHEAAIC YNTPGSFSCR CQPGYRGDGF
960 970 980 990 1000
HCTSDTVPED SISGLKPCEY QQRYAQTQHA YPGSRIHIPQ CDDQGNFVPL
1010 1020 1030 1040 1050
QCHGSTGFCW CVDRNGHEVP GTQTPPGSTP PHCGPPPEPT QRPRTVCERW
1060 1070 1080 1090 1100
RESLLEHYGG TPRDDQYVPQ CDDLGHFIPL QCHGKSDFCW CVDKDGRELQ
1110 1120 1130 1140 1150
GTRSQPGTRP ACIPTVAPPV VRPTPRPDVT PPSVGTFLLY AQGQQIGHLP
1160 1170 1180 1190 1200
LNGSRLQKDA ARTLLSLHGS IVVGIDYDCR ERMVYWTDVA GRTISRASLE
1210 1220 1230 1240 1250
AGAEPETIIT SGLISPEGLA IDHFRRTMYW TDSGLDKIER AELDGSERKV
1260 1270 1280 1290 1300
LFHTDLVNPR AITVDPIRGN LYWTDWNREA PKIETSSLDG ENRRILINKD
1310 1320 1330 1340 1350
IGLPNGLTFD PFSKLLCWAD AGTKKLECTL PDGTGRRVIQ NHLNYPFSIV
1360 1370 1380 1390 1400
SYADHFYHTD WRRDGVISVN KDSGQFTDEF LPEQRSHLYG ITAVYPYCPT

GRK
Length:1,403
Mass (Da):153,913
Last modified:July 27, 2011 - v2
Checksum:iABA0B1DB2C84A27A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971L → F in BAA32609 (PubMed:9633511).Curated
Sequence conflicti251 – 2511S → F in BAA32609 (PubMed:9633511).Curated
Sequence conflicti367 – 3671A → V in BAA32609 (PubMed:9633511).Curated
Sequence conflicti375 – 3751P → H in BAA32609 (PubMed:9633511).Curated
Sequence conflicti386 – 3861S → W in BAA32609 (PubMed:9633511).Curated
Sequence conflicti496 – 4961S → F in BAA32609 (PubMed:9633511).Curated
Sequence conflicti651 – 6511R → C in BAA32609 (PubMed:9633511).Curated
Sequence conflicti876 – 8761L → F in BAA32609 (PubMed:9633511).Curated
Sequence conflicti1151 – 11511L → F in BAA32609 (PubMed:9633511).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017202 mRNA. Translation: BAA32609.1.
CH466613 Genomic DNA. Translation: EDL01537.1.
BC054746 mRNA. Translation: AAH54746.1.
BC057016 mRNA. Translation: AAH57016.1.
CCDSiCCDS36813.1.
RefSeqiNP_032721.2. NM_008695.2.
UniGeneiMm.20348.

Genome annotation databases

EnsembliENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806.
GeneIDi18074.
KEGGimmu:18074.
UCSCiuc007siu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017202 mRNA. Translation: BAA32609.1.
CH466613 Genomic DNA. Translation: EDL01537.1.
BC054746 mRNA. Translation: AAH54746.1.
BC057016 mRNA. Translation: AAH57016.1.
CCDSiCCDS36813.1.
RefSeqiNP_032721.2. NM_008695.2.
UniGeneiMm.20348.

3D structure databases

ProteinModelPortaliO88322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201771. 4 interactions.
STRINGi10090.ENSMUSP00000022340.

Protein family/group databases

MEROPSiI63.001.

Proteomic databases

MaxQBiO88322.
PaxDbiO88322.
PRIDEiO88322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806.
GeneIDi18074.
KEGGimmu:18074.
UCSCiuc007siu.1. mouse.

Organism-specific databases

CTDi22795.
MGIiMGI:1298229. Nid2.

Phylogenomic databases

eggNOGiNOG282695.
GeneTreeiENSGT00760000118968.
HOGENOMiHOG000072712.
HOVERGENiHBG006498.
InParanoidiO88322.
KOiK06826.
OMAiARVGFCR.
OrthoDBiEOG7M0NQN.
TreeFamiTF320666.

Enzyme and pathway databases

ReactomeiREACT_286043. Laminin interactions.

Miscellaneous databases

NextBioi293203.
PROiO88322.
SOURCEiSearch...

Gene expression databases

BgeeiO88322.
CleanExiMM_NID2.
GenevisibleiO88322. MM.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.40.155.10. 2 hits.
4.10.800.10. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR006605. G2_nidogen/fibulin_G2F.
IPR009017. GFP.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR003886. Nidogen_extracell_dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07645. EGF_CA. 2 hits.
PF07474. G2F. 1 hit.
PF00058. Ldl_recept_b. 2 hits.
PF06119. NIDO. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00682. G2F. 1 hit.
SM00135. LY. 4 hits.
SM00539. NIDO. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF54511. SSF54511. 1 hit.
SSF57184. SSF57184. 2 hits.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 2 hits.
PS51120. LDLRB. 4 hits.
PS51220. NIDO. 1 hit.
PS50993. NIDOGEN_G2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Entactin-2: a new member of basement membrane protein with high homology to entactin/nidogen."
    Kimura N., Toyoshima T., Kojima T., Shimane M.
    Exp. Cell Res. 241:36-45(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins."
    Talts J.F., Andac Z., Goehring W., Brancaccio A., Timpl R.
    EMBO J. 18:863-870(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAMA2.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-681.

Entry informationi

Entry nameiNID2_MOUSE
AccessioniPrimary (citable) accession number: O88322
Secondary accession number(s): Q7TQF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.