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O88322

- NID2_MOUSE

UniProt

O88322 - NID2_MOUSE

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Protein

Nidogen-2

Gene
Nid2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. protein binding Source: MGI

GO - Biological processi

  1. cell-matrix adhesion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_202342. Laminin interactions.

Protein family/group databases

MEROPSiI63.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Nidogen-2
Short name:
NID-2
Alternative name(s):
Entactin-2
Gene namesi
Name:Nid2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1298229. Nid2.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: MGI
  2. cell surface Source: Ensembl
  3. extracellular region Source: Reactome
  4. proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030 By similarityAdd
BLAST
Chaini31 – 14031373Nidogen-2PRO_0000007672Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi511 ↔ 524 By similarity
Disulfide bondi518 ↔ 533 By similarity
Disulfide bondi535 ↔ 546 By similarity
Glycosylationi681 – 6811N-linked (GlcNAc...)1 Publication
Glycosylationi716 – 7161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi726 – 7261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi786 ↔ 799 By similarity
Disulfide bondi793 ↔ 809 By similarity
Disulfide bondi811 ↔ 822 By similarity
Disulfide bondi828 ↔ 841 By similarity
Disulfide bondi835 ↔ 850 By similarity
Disulfide bondi852 ↔ 865 By similarity
Disulfide bondi875 ↔ 890 By similarity
Disulfide bondi882 ↔ 900 By similarity
Disulfide bondi902 ↔ 913 By similarity
Disulfide bondi919 ↔ 930 By similarity
Disulfide bondi924 ↔ 939 By similarity
Disulfide bondi941 ↔ 952 By similarity
Disulfide bondi968 ↔ 991 By similarity
Disulfide bondi1002 ↔ 1009 By similarity
Disulfide bondi1011 ↔ 1033 By similarity
Disulfide bondi1047 ↔ 1071 By similarity
Disulfide bondi1082 ↔ 1089 By similarity
Disulfide bondi1091 ↔ 1112 By similarity
Glycosylationi1152 – 11521N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Highly N- and O-glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO88322.
PaxDbiO88322.
PRIDEiO88322.

Expressioni

Gene expression databases

BgeeiO88322.
CleanExiMM_NID2.
GenevestigatoriO88322.

Interactioni

Subunit structurei

Interacts with LAMA2. Interacts with COL13A1 By similarity.1 Publication

Protein-protein interaction databases

BioGridi201771. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliO88322.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 274167NIDOAdd
BLAST
Domaini507 – 54741EGF-like 1Add
BLAST
Domaini551 – 781231Nidogen G2 beta-barrelAdd
BLAST
Domaini782 – 82342EGF-like 2Add
BLAST
Domaini824 – 86239EGF-like 3; calcium-binding Reviewed predictionAdd
BLAST
Domaini871 – 91444EGF-like 4Add
BLAST
Domaini915 – 95339EGF-like 5; calcium-binding Reviewed predictionAdd
BLAST
Domaini965 – 103369Thyroglobulin type-1 1Add
BLAST
Domaini1044 – 111269Thyroglobulin type-1 2Add
BLAST
Repeati1182 – 122544LDL-receptor class B 1Add
BLAST
Repeati1226 – 126843LDL-receptor class B 2Add
BLAST
Repeati1269 – 131345LDL-receptor class B 3Add
BLAST
Repeati1314 – 135542LDL-receptor class B 4Add
BLAST
Repeati1357 – 140145LDL-receptor class B 5Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi946 – 9483Cell attachment site

Sequence similaritiesi

Contains 5 EGF-like domains.
Contains 1 NIDO domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG282695.
GeneTreeiENSGT00750000117524.
HOGENOMiHOG000072712.
HOVERGENiHBG006498.
InParanoidiQ7TQF0.
KOiK06826.
OMAiARVGFCR.
OrthoDBiEOG7M0NQN.
TreeFamiTF320666.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.40.155.10. 2 hits.
4.10.800.10. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR006605. G2_nidogen/fibulin_G2F.
IPR009017. GFP.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR003886. Nidogen_extracell_dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07645. EGF_CA. 2 hits.
PF07474. G2F. 1 hit.
PF00058. Ldl_recept_b. 2 hits.
PF06119. NIDO. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00682. G2F. 1 hit.
SM00135. LY. 4 hits.
SM00539. NIDO. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF54511. SSF54511. 1 hit.
SSF57184. SSF57184. 2 hits.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 2 hits.
PS51120. LDLRB. 4 hits.
PS51220. NIDO. 1 hit.
PS50993. NIDOGEN_G2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88322-1 [UniParc]FASTAAdd to Basket

« Hide

MFRDPTAGWL TPPSPLSLLV MLLLLSRVGA LRPDELFPYG ESWGDQLLPE     50
GDDESSAAVK LAIPLRFYDA QFSSLYVGTN GIISTQDFPR ETQYVDDDFP 100
TDFPAIAPFL ADIDTSHSRG RILYREDTSG AVLSLAARYV RTGFPLSGSS 150
FTPTHAFLAT WEHVGAYEEV SRGAAPSGEL NTFQAVLASD ESDTYALFLY 200
PANGLQFFGT RPKESYNVQL QLPARVGFCR GEADDLKREA LYFSLTNTEQ 250
SVKNLYQLSN LGIPGVWAFH IGSRFALDNV RPATVGGDPS TARSSALEHP 300
FSHAAALESY TEDSFHYYDE NEEDVEYPPV EPGEAPEGHS RIDVSFNSKA 350
DPGLVDVGTS SPGSDRASPW PYPAPGNWPS YRETESASLD PQTKQGRPVG 400
EGEVLDFRDP AELLDQMGTR APAPPEADAA LLTPVNEDLG GRNTQSYPEA 450
GPVPSEPDVP VPPLEGEVLP HYPESGHVPP LRGGKYVIGL EDHVGSNDQV 500
FTYNGANLET CEHSHGRCSQ HAFCTDYTTG FCCHCQSRFY GNGKHCLPEG 550
APHRVNGKVS GRLRVGHIPV HFTDVDLHAY IVGNDGRAYT AISHVPQPAA 600
QALLPVLPIG GLFGWLFALE KPGSENGFSL TGATFVHDVE VTFHPGEERV 650
RITQTAEGLD PENYLSIKTN IEGQVPFIPA NFTAHITPYK EFYHYRDSVV 700
TSSSSRSFSL TSGSINQTWS YHIDQNITYQ ACRHAPRHLA IPATQQLTVD 750
RAFALYSEDE GVLRFAVTNQ IGPVEVDSAP VGVNPCYDGS HTCDTTARCH 800
PGTGVDYTCE CTPGFQGDGR SCVDVNECAT GFHRCGPNSV CVNLVGSYRC 850
ECRSGYEFAD DQHTCILIAP PPNPCLDGSH TCAPEGQARC IHHGGSSFSC 900
ACLPGFIGTG HQCSDVDECA ENRCHEAAIC YNTPGSFSCR CQPGYRGDGF 950
HCTSDTVPED SISGLKPCEY QQRYAQTQHA YPGSRIHIPQ CDDQGNFVPL 1000
QCHGSTGFCW CVDRNGHEVP GTQTPPGSTP PHCGPPPEPT QRPRTVCERW 1050
RESLLEHYGG TPRDDQYVPQ CDDLGHFIPL QCHGKSDFCW CVDKDGRELQ 1100
GTRSQPGTRP ACIPTVAPPV VRPTPRPDVT PPSVGTFLLY AQGQQIGHLP 1150
LNGSRLQKDA ARTLLSLHGS IVVGIDYDCR ERMVYWTDVA GRTISRASLE 1200
AGAEPETIIT SGLISPEGLA IDHFRRTMYW TDSGLDKIER AELDGSERKV 1250
LFHTDLVNPR AITVDPIRGN LYWTDWNREA PKIETSSLDG ENRRILINKD 1300
IGLPNGLTFD PFSKLLCWAD AGTKKLECTL PDGTGRRVIQ NHLNYPFSIV 1350
SYADHFYHTD WRRDGVISVN KDSGQFTDEF LPEQRSHLYG ITAVYPYCPT 1400
GRK 1403
Length:1,403
Mass (Da):153,913
Last modified:July 27, 2011 - v2
Checksum:iABA0B1DB2C84A27A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971L → F in BAA32609. 1 Publication
Sequence conflicti251 – 2511S → F in BAA32609. 1 Publication
Sequence conflicti367 – 3671A → V in BAA32609. 1 Publication
Sequence conflicti375 – 3751P → H in BAA32609. 1 Publication
Sequence conflicti386 – 3861S → W in BAA32609. 1 Publication
Sequence conflicti496 – 4961S → F in BAA32609. 1 Publication
Sequence conflicti651 – 6511R → C in BAA32609. 1 Publication
Sequence conflicti876 – 8761L → F in BAA32609. 1 Publication
Sequence conflicti1151 – 11511L → F in BAA32609. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017202 mRNA. Translation: BAA32609.1.
CH466613 Genomic DNA. Translation: EDL01537.1.
BC054746 mRNA. Translation: AAH54746.1.
BC057016 mRNA. Translation: AAH57016.1.
CCDSiCCDS36813.1.
RefSeqiNP_032721.2. NM_008695.2.
UniGeneiMm.20348.

Genome annotation databases

EnsembliENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806.
GeneIDi18074.
KEGGimmu:18074.
UCSCiuc007siu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017202 mRNA. Translation: BAA32609.1 .
CH466613 Genomic DNA. Translation: EDL01537.1 .
BC054746 mRNA. Translation: AAH54746.1 .
BC057016 mRNA. Translation: AAH57016.1 .
CCDSi CCDS36813.1.
RefSeqi NP_032721.2. NM_008695.2.
UniGenei Mm.20348.

3D structure databases

ProteinModelPortali O88322.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201771. 4 interactions.

Protein family/group databases

MEROPSi I63.001.

Proteomic databases

MaxQBi O88322.
PaxDbi O88322.
PRIDEi O88322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022340 ; ENSMUSP00000022340 ; ENSMUSG00000021806 .
GeneIDi 18074.
KEGGi mmu:18074.
UCSCi uc007siu.1. mouse.

Organism-specific databases

CTDi 22795.
MGIi MGI:1298229. Nid2.

Phylogenomic databases

eggNOGi NOG282695.
GeneTreei ENSGT00750000117524.
HOGENOMi HOG000072712.
HOVERGENi HBG006498.
InParanoidi Q7TQF0.
KOi K06826.
OMAi ARVGFCR.
OrthoDBi EOG7M0NQN.
TreeFami TF320666.

Enzyme and pathway databases

Reactomei REACT_202342. Laminin interactions.

Miscellaneous databases

NextBioi 293203.
PROi O88322.
SOURCEi Search...

Gene expression databases

Bgeei O88322.
CleanExi MM_NID2.
Genevestigatori O88322.

Family and domain databases

Gene3Di 2.120.10.30. 1 hit.
2.40.155.10. 2 hits.
4.10.800.10. 2 hits.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR006605. G2_nidogen/fibulin_G2F.
IPR009017. GFP.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR003886. Nidogen_extracell_dom.
IPR000716. Thyroglobulin_1.
[Graphical view ]
Pfami PF07645. EGF_CA. 2 hits.
PF07474. G2F. 1 hit.
PF00058. Ldl_recept_b. 2 hits.
PF06119. NIDO. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view ]
SMARTi SM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00682. G2F. 1 hit.
SM00135. LY. 4 hits.
SM00539. NIDO. 1 hit.
SM00211. TY. 2 hits.
[Graphical view ]
SUPFAMi SSF54511. SSF54511. 1 hit.
SSF57184. SSF57184. 2 hits.
SSF57610. SSF57610. 2 hits.
PROSITEi PS00010. ASX_HYDROXYL. 3 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 2 hits.
PS51120. LDLRB. 4 hits.
PS51220. NIDO. 1 hit.
PS50993. NIDOGEN_G2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Entactin-2: a new member of basement membrane protein with high homology to entactin/nidogen."
    Kimura N., Toyoshima T., Kojima T., Shimane M.
    Exp. Cell Res. 241:36-45(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins."
    Talts J.F., Andac Z., Goehring W., Brancaccio A., Timpl R.
    EMBO J. 18:863-870(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAMA2.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-681.

Entry informationi

Entry nameiNID2_MOUSE
AccessioniPrimary (citable) accession number: O88322
Secondary accession number(s): Q7TQF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi