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O88322 (NID2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nidogen-2

Short name=NID-2
Alternative name(s):
Entactin-2
Gene names
Name:Nid2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1403 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.

Subunit structure

Interacts with LAMA2. Interacts with COL13A1 By similarity. Ref.4

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane.

Post-translational modification

Highly N- and O-glycosylated By similarity.

Sequence similarities

Contains 5 EGF-like domains.

Contains 5 LDL-receptor class B repeats.

Contains 1 NIDO domain.

Contains 1 nidogen G2 beta-barrel domain.

Contains 2 thyroglobulin type-1 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 By similarity
Chain31 – 14031373Nidogen-2
PRO_0000007672

Regions

Domain108 – 274167NIDO
Domain507 – 54741EGF-like 1
Domain551 – 781231Nidogen G2 beta-barrel
Domain782 – 82342EGF-like 2
Domain824 – 86239EGF-like 3; calcium-binding Potential
Domain871 – 91444EGF-like 4
Domain915 – 95339EGF-like 5; calcium-binding Potential
Domain965 – 103369Thyroglobulin type-1 1
Domain1044 – 111269Thyroglobulin type-1 2
Repeat1182 – 122544LDL-receptor class B 1
Repeat1226 – 126843LDL-receptor class B 2
Repeat1269 – 131345LDL-receptor class B 3
Repeat1314 – 135542LDL-receptor class B 4
Repeat1357 – 140145LDL-receptor class B 5
Motif946 – 9483Cell attachment site

Amino acid modifications

Glycosylation6811N-linked (GlcNAc...) Ref.5
Glycosylation7161N-linked (GlcNAc...) Potential
Glycosylation7261N-linked (GlcNAc...) Potential
Glycosylation11521N-linked (GlcNAc...) Potential
Disulfide bond511 ↔ 524 By similarity
Disulfide bond518 ↔ 533 By similarity
Disulfide bond535 ↔ 546 By similarity
Disulfide bond786 ↔ 799 By similarity
Disulfide bond793 ↔ 809 By similarity
Disulfide bond811 ↔ 822 By similarity
Disulfide bond828 ↔ 841 By similarity
Disulfide bond835 ↔ 850 By similarity
Disulfide bond852 ↔ 865 By similarity
Disulfide bond875 ↔ 890 By similarity
Disulfide bond882 ↔ 900 By similarity
Disulfide bond902 ↔ 913 By similarity
Disulfide bond919 ↔ 930 By similarity
Disulfide bond924 ↔ 939 By similarity
Disulfide bond941 ↔ 952 By similarity
Disulfide bond968 ↔ 991 By similarity
Disulfide bond1002 ↔ 1009 By similarity
Disulfide bond1011 ↔ 1033 By similarity
Disulfide bond1047 ↔ 1071 By similarity
Disulfide bond1082 ↔ 1089 By similarity
Disulfide bond1091 ↔ 1112 By similarity

Experimental info

Sequence conflict1971L → F in BAA32609. Ref.1
Sequence conflict2511S → F in BAA32609. Ref.1
Sequence conflict3671A → V in BAA32609. Ref.1
Sequence conflict3751P → H in BAA32609. Ref.1
Sequence conflict3861S → W in BAA32609. Ref.1
Sequence conflict4961S → F in BAA32609. Ref.1
Sequence conflict6511R → C in BAA32609. Ref.1
Sequence conflict8761L → F in BAA32609. Ref.1
Sequence conflict11511L → F in BAA32609. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O88322 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: ABA0B1DB2C84A27A

FASTA1,403153,913
        10         20         30         40         50         60 
MFRDPTAGWL TPPSPLSLLV MLLLLSRVGA LRPDELFPYG ESWGDQLLPE GDDESSAAVK 

        70         80         90        100        110        120 
LAIPLRFYDA QFSSLYVGTN GIISTQDFPR ETQYVDDDFP TDFPAIAPFL ADIDTSHSRG 

       130        140        150        160        170        180 
RILYREDTSG AVLSLAARYV RTGFPLSGSS FTPTHAFLAT WEHVGAYEEV SRGAAPSGEL 

       190        200        210        220        230        240 
NTFQAVLASD ESDTYALFLY PANGLQFFGT RPKESYNVQL QLPARVGFCR GEADDLKREA 

       250        260        270        280        290        300 
LYFSLTNTEQ SVKNLYQLSN LGIPGVWAFH IGSRFALDNV RPATVGGDPS TARSSALEHP 

       310        320        330        340        350        360 
FSHAAALESY TEDSFHYYDE NEEDVEYPPV EPGEAPEGHS RIDVSFNSKA DPGLVDVGTS 

       370        380        390        400        410        420 
SPGSDRASPW PYPAPGNWPS YRETESASLD PQTKQGRPVG EGEVLDFRDP AELLDQMGTR 

       430        440        450        460        470        480 
APAPPEADAA LLTPVNEDLG GRNTQSYPEA GPVPSEPDVP VPPLEGEVLP HYPESGHVPP 

       490        500        510        520        530        540 
LRGGKYVIGL EDHVGSNDQV FTYNGANLET CEHSHGRCSQ HAFCTDYTTG FCCHCQSRFY 

       550        560        570        580        590        600 
GNGKHCLPEG APHRVNGKVS GRLRVGHIPV HFTDVDLHAY IVGNDGRAYT AISHVPQPAA 

       610        620        630        640        650        660 
QALLPVLPIG GLFGWLFALE KPGSENGFSL TGATFVHDVE VTFHPGEERV RITQTAEGLD 

       670        680        690        700        710        720 
PENYLSIKTN IEGQVPFIPA NFTAHITPYK EFYHYRDSVV TSSSSRSFSL TSGSINQTWS 

       730        740        750        760        770        780 
YHIDQNITYQ ACRHAPRHLA IPATQQLTVD RAFALYSEDE GVLRFAVTNQ IGPVEVDSAP 

       790        800        810        820        830        840 
VGVNPCYDGS HTCDTTARCH PGTGVDYTCE CTPGFQGDGR SCVDVNECAT GFHRCGPNSV 

       850        860        870        880        890        900 
CVNLVGSYRC ECRSGYEFAD DQHTCILIAP PPNPCLDGSH TCAPEGQARC IHHGGSSFSC 

       910        920        930        940        950        960 
ACLPGFIGTG HQCSDVDECA ENRCHEAAIC YNTPGSFSCR CQPGYRGDGF HCTSDTVPED 

       970        980        990       1000       1010       1020 
SISGLKPCEY QQRYAQTQHA YPGSRIHIPQ CDDQGNFVPL QCHGSTGFCW CVDRNGHEVP 

      1030       1040       1050       1060       1070       1080 
GTQTPPGSTP PHCGPPPEPT QRPRTVCERW RESLLEHYGG TPRDDQYVPQ CDDLGHFIPL 

      1090       1100       1110       1120       1130       1140 
QCHGKSDFCW CVDKDGRELQ GTRSQPGTRP ACIPTVAPPV VRPTPRPDVT PPSVGTFLLY 

      1150       1160       1170       1180       1190       1200 
AQGQQIGHLP LNGSRLQKDA ARTLLSLHGS IVVGIDYDCR ERMVYWTDVA GRTISRASLE 

      1210       1220       1230       1240       1250       1260 
AGAEPETIIT SGLISPEGLA IDHFRRTMYW TDSGLDKIER AELDGSERKV LFHTDLVNPR 

      1270       1280       1290       1300       1310       1320 
AITVDPIRGN LYWTDWNREA PKIETSSLDG ENRRILINKD IGLPNGLTFD PFSKLLCWAD 

      1330       1340       1350       1360       1370       1380 
AGTKKLECTL PDGTGRRVIQ NHLNYPFSIV SYADHFYHTD WRRDGVISVN KDSGQFTDEF 

      1390       1400 
LPEQRSHLYG ITAVYPYCPT GRK 

« Hide

References

« Hide 'large scale' references
[1]"Entactin-2: a new member of basement membrane protein with high homology to entactin/nidogen."
Kimura N., Toyoshima T., Kojima T., Shimane M.
Exp. Cell Res. 241:36-45(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins."
Talts J.F., Andac Z., Goehring W., Brancaccio A., Timpl R.
EMBO J. 18:863-870(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LAMA2.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-681.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017202 mRNA. Translation: BAA32609.1.
CH466613 Genomic DNA. Translation: EDL01537.1.
BC054746 mRNA. Translation: AAH54746.1.
BC057016 mRNA. Translation: AAH57016.1.
CCDSCCDS36813.1.
RefSeqNP_032721.2. NM_008695.2.
UniGeneMm.20348.

3D structure databases

ProteinModelPortalO88322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201771. 4 interactions.

Protein family/group databases

MEROPSI63.001.

Proteomic databases

MaxQBO88322.
PaxDbO88322.
PRIDEO88322.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806.
GeneID18074.
KEGGmmu:18074.
UCSCuc007siu.1. mouse.

Organism-specific databases

CTD22795.
MGIMGI:1298229. Nid2.

Phylogenomic databases

eggNOGNOG282695.
GeneTreeENSGT00750000117524.
HOGENOMHOG000072712.
HOVERGENHBG006498.
InParanoidQ7TQF0.
KOK06826.
OMAARVGFCR.
OrthoDBEOG7M0NQN.
TreeFamTF320666.

Enzyme and pathway databases

ReactomeREACT_206066. Extracellular matrix organization.

Gene expression databases

BgeeO88322.
CleanExMM_NID2.
GenevestigatorO88322.

Family and domain databases

Gene3D2.120.10.30. 1 hit.
2.40.155.10. 2 hits.
4.10.800.10. 2 hits.
InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR006605. G2_nidogen/fibulin_G2F.
IPR009017. GFP.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR003886. Nidogen_extracell_dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamPF07645. EGF_CA. 2 hits.
PF07474. G2F. 1 hit.
PF00058. Ldl_recept_b. 2 hits.
PF06119. NIDO. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00682. G2F. 1 hit.
SM00135. LY. 4 hits.
SM00539. NIDO. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMSSF54511. SSF54511. 1 hit.
SSF57184. SSF57184. 2 hits.
SSF57610. SSF57610. 2 hits.
PROSITEPS00010. ASX_HYDROXYL. 3 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 2 hits.
PS51120. LDLRB. 4 hits.
PS51220. NIDO. 1 hit.
PS50993. NIDOGEN_G2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio293203.
PROO88322.
SOURCESearch...

Entry information

Entry nameNID2_MOUSE
AccessionPrimary (citable) accession number: O88322
Secondary accession number(s): Q7TQF0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot