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O88322

- NID2_MOUSE

UniProt

O88322 - NID2_MOUSE

Protein

Nidogen-2

Gene

Nid2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. protein binding Source: MGI

    GO - Biological processi

    1. cell-matrix adhesion Source: MGI

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_202342. Laminin interactions.

    Protein family/group databases

    MEROPSiI63.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nidogen-2
    Short name:
    NID-2
    Alternative name(s):
    Entactin-2
    Gene namesi
    Name:Nid2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1298229. Nid2.

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: MGI
    2. cell surface Source: Ensembl
    3. extracellular region Source: Reactome
    4. proteinaceous extracellular matrix Source: MGI

    Keywords - Cellular componenti

    Basement membrane, Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030By similarityAdd
    BLAST
    Chaini31 – 14031373Nidogen-2PRO_0000007672Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi511 ↔ 524By similarity
    Disulfide bondi518 ↔ 533By similarity
    Disulfide bondi535 ↔ 546By similarity
    Glycosylationi681 – 6811N-linked (GlcNAc...)1 Publication
    Glycosylationi716 – 7161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi726 – 7261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi786 ↔ 799By similarity
    Disulfide bondi793 ↔ 809By similarity
    Disulfide bondi811 ↔ 822By similarity
    Disulfide bondi828 ↔ 841By similarity
    Disulfide bondi835 ↔ 850By similarity
    Disulfide bondi852 ↔ 865By similarity
    Disulfide bondi875 ↔ 890By similarity
    Disulfide bondi882 ↔ 900By similarity
    Disulfide bondi902 ↔ 913By similarity
    Disulfide bondi919 ↔ 930By similarity
    Disulfide bondi924 ↔ 939By similarity
    Disulfide bondi941 ↔ 952By similarity
    Disulfide bondi968 ↔ 991By similarity
    Disulfide bondi1002 ↔ 1009By similarity
    Disulfide bondi1011 ↔ 1033By similarity
    Disulfide bondi1047 ↔ 1071By similarity
    Disulfide bondi1082 ↔ 1089By similarity
    Disulfide bondi1091 ↔ 1112By similarity
    Glycosylationi1152 – 11521N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Highly N- and O-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO88322.
    PaxDbiO88322.
    PRIDEiO88322.

    Expressioni

    Gene expression databases

    BgeeiO88322.
    CleanExiMM_NID2.
    GenevestigatoriO88322.

    Interactioni

    Subunit structurei

    Interacts with LAMA2. Interacts with COL13A1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201771. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO88322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 274167NIDOPROSITE-ProRule annotationAdd
    BLAST
    Domaini507 – 54741EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini551 – 781231Nidogen G2 beta-barrelPROSITE-ProRule annotationAdd
    BLAST
    Domaini782 – 82342EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini824 – 86239EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini871 – 91444EGF-like 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini915 – 95339EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini965 – 103369Thyroglobulin type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1044 – 111269Thyroglobulin type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati1182 – 122544LDL-receptor class B 1Add
    BLAST
    Repeati1226 – 126843LDL-receptor class B 2Add
    BLAST
    Repeati1269 – 131345LDL-receptor class B 3Add
    BLAST
    Repeati1314 – 135542LDL-receptor class B 4Add
    BLAST
    Repeati1357 – 140145LDL-receptor class B 5Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi946 – 9483Cell attachment site

    Sequence similaritiesi

    Contains 5 EGF-like domains.PROSITE-ProRule annotation
    Contains 5 LDL-receptor class B repeats.PROSITE-ProRule annotation
    Contains 1 NIDO domain.PROSITE-ProRule annotation
    Contains 1 nidogen G2 beta-barrel domain.PROSITE-ProRule annotation
    Contains 2 thyroglobulin type-1 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG282695.
    GeneTreeiENSGT00750000117524.
    HOGENOMiHOG000072712.
    HOVERGENiHBG006498.
    InParanoidiQ7TQF0.
    KOiK06826.
    OMAiARVGFCR.
    OrthoDBiEOG7M0NQN.
    TreeFamiTF320666.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    2.40.155.10. 2 hits.
    4.10.800.10. 2 hits.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR006605. G2_nidogen/fibulin_G2F.
    IPR009017. GFP.
    IPR023413. GFP_like.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000033. LDLR_classB_rpt.
    IPR003886. Nidogen_extracell_dom.
    IPR000716. Thyroglobulin_1.
    [Graphical view]
    PfamiPF07645. EGF_CA. 2 hits.
    PF07474. G2F. 1 hit.
    PF00058. Ldl_recept_b. 2 hits.
    PF06119. NIDO. 1 hit.
    PF00086. Thyroglobulin_1. 2 hits.
    [Graphical view]
    SMARTiSM00181. EGF. 3 hits.
    SM00179. EGF_CA. 2 hits.
    SM00682. G2F. 1 hit.
    SM00135. LY. 4 hits.
    SM00539. NIDO. 1 hit.
    SM00211. TY. 2 hits.
    [Graphical view]
    SUPFAMiSSF54511. SSF54511. 1 hit.
    SSF57184. SSF57184. 2 hits.
    SSF57610. SSF57610. 2 hits.
    PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 5 hits.
    PS01187. EGF_CA. 2 hits.
    PS51120. LDLRB. 4 hits.
    PS51220. NIDO. 1 hit.
    PS50993. NIDOGEN_G2. 1 hit.
    PS00484. THYROGLOBULIN_1_1. 2 hits.
    PS51162. THYROGLOBULIN_1_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88322-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRDPTAGWL TPPSPLSLLV MLLLLSRVGA LRPDELFPYG ESWGDQLLPE     50
    GDDESSAAVK LAIPLRFYDA QFSSLYVGTN GIISTQDFPR ETQYVDDDFP 100
    TDFPAIAPFL ADIDTSHSRG RILYREDTSG AVLSLAARYV RTGFPLSGSS 150
    FTPTHAFLAT WEHVGAYEEV SRGAAPSGEL NTFQAVLASD ESDTYALFLY 200
    PANGLQFFGT RPKESYNVQL QLPARVGFCR GEADDLKREA LYFSLTNTEQ 250
    SVKNLYQLSN LGIPGVWAFH IGSRFALDNV RPATVGGDPS TARSSALEHP 300
    FSHAAALESY TEDSFHYYDE NEEDVEYPPV EPGEAPEGHS RIDVSFNSKA 350
    DPGLVDVGTS SPGSDRASPW PYPAPGNWPS YRETESASLD PQTKQGRPVG 400
    EGEVLDFRDP AELLDQMGTR APAPPEADAA LLTPVNEDLG GRNTQSYPEA 450
    GPVPSEPDVP VPPLEGEVLP HYPESGHVPP LRGGKYVIGL EDHVGSNDQV 500
    FTYNGANLET CEHSHGRCSQ HAFCTDYTTG FCCHCQSRFY GNGKHCLPEG 550
    APHRVNGKVS GRLRVGHIPV HFTDVDLHAY IVGNDGRAYT AISHVPQPAA 600
    QALLPVLPIG GLFGWLFALE KPGSENGFSL TGATFVHDVE VTFHPGEERV 650
    RITQTAEGLD PENYLSIKTN IEGQVPFIPA NFTAHITPYK EFYHYRDSVV 700
    TSSSSRSFSL TSGSINQTWS YHIDQNITYQ ACRHAPRHLA IPATQQLTVD 750
    RAFALYSEDE GVLRFAVTNQ IGPVEVDSAP VGVNPCYDGS HTCDTTARCH 800
    PGTGVDYTCE CTPGFQGDGR SCVDVNECAT GFHRCGPNSV CVNLVGSYRC 850
    ECRSGYEFAD DQHTCILIAP PPNPCLDGSH TCAPEGQARC IHHGGSSFSC 900
    ACLPGFIGTG HQCSDVDECA ENRCHEAAIC YNTPGSFSCR CQPGYRGDGF 950
    HCTSDTVPED SISGLKPCEY QQRYAQTQHA YPGSRIHIPQ CDDQGNFVPL 1000
    QCHGSTGFCW CVDRNGHEVP GTQTPPGSTP PHCGPPPEPT QRPRTVCERW 1050
    RESLLEHYGG TPRDDQYVPQ CDDLGHFIPL QCHGKSDFCW CVDKDGRELQ 1100
    GTRSQPGTRP ACIPTVAPPV VRPTPRPDVT PPSVGTFLLY AQGQQIGHLP 1150
    LNGSRLQKDA ARTLLSLHGS IVVGIDYDCR ERMVYWTDVA GRTISRASLE 1200
    AGAEPETIIT SGLISPEGLA IDHFRRTMYW TDSGLDKIER AELDGSERKV 1250
    LFHTDLVNPR AITVDPIRGN LYWTDWNREA PKIETSSLDG ENRRILINKD 1300
    IGLPNGLTFD PFSKLLCWAD AGTKKLECTL PDGTGRRVIQ NHLNYPFSIV 1350
    SYADHFYHTD WRRDGVISVN KDSGQFTDEF LPEQRSHLYG ITAVYPYCPT 1400
    GRK 1403
    Length:1,403
    Mass (Da):153,913
    Last modified:July 27, 2011 - v2
    Checksum:iABA0B1DB2C84A27A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti197 – 1971L → F in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti251 – 2511S → F in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti367 – 3671A → V in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti375 – 3751P → H in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti386 – 3861S → W in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti496 – 4961S → F in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti651 – 6511R → C in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti876 – 8761L → F in BAA32609. (PubMed:9633511)Curated
    Sequence conflicti1151 – 11511L → F in BAA32609. (PubMed:9633511)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017202 mRNA. Translation: BAA32609.1.
    CH466613 Genomic DNA. Translation: EDL01537.1.
    BC054746 mRNA. Translation: AAH54746.1.
    BC057016 mRNA. Translation: AAH57016.1.
    CCDSiCCDS36813.1.
    RefSeqiNP_032721.2. NM_008695.2.
    UniGeneiMm.20348.

    Genome annotation databases

    EnsembliENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806.
    GeneIDi18074.
    KEGGimmu:18074.
    UCSCiuc007siu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017202 mRNA. Translation: BAA32609.1 .
    CH466613 Genomic DNA. Translation: EDL01537.1 .
    BC054746 mRNA. Translation: AAH54746.1 .
    BC057016 mRNA. Translation: AAH57016.1 .
    CCDSi CCDS36813.1.
    RefSeqi NP_032721.2. NM_008695.2.
    UniGenei Mm.20348.

    3D structure databases

    ProteinModelPortali O88322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201771. 4 interactions.

    Protein family/group databases

    MEROPSi I63.001.

    Proteomic databases

    MaxQBi O88322.
    PaxDbi O88322.
    PRIDEi O88322.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022340 ; ENSMUSP00000022340 ; ENSMUSG00000021806 .
    GeneIDi 18074.
    KEGGi mmu:18074.
    UCSCi uc007siu.1. mouse.

    Organism-specific databases

    CTDi 22795.
    MGIi MGI:1298229. Nid2.

    Phylogenomic databases

    eggNOGi NOG282695.
    GeneTreei ENSGT00750000117524.
    HOGENOMi HOG000072712.
    HOVERGENi HBG006498.
    InParanoidi Q7TQF0.
    KOi K06826.
    OMAi ARVGFCR.
    OrthoDBi EOG7M0NQN.
    TreeFami TF320666.

    Enzyme and pathway databases

    Reactomei REACT_202342. Laminin interactions.

    Miscellaneous databases

    NextBioi 293203.
    PROi O88322.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88322.
    CleanExi MM_NID2.
    Genevestigatori O88322.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    2.40.155.10. 2 hits.
    4.10.800.10. 2 hits.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR006605. G2_nidogen/fibulin_G2F.
    IPR009017. GFP.
    IPR023413. GFP_like.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000033. LDLR_classB_rpt.
    IPR003886. Nidogen_extracell_dom.
    IPR000716. Thyroglobulin_1.
    [Graphical view ]
    Pfami PF07645. EGF_CA. 2 hits.
    PF07474. G2F. 1 hit.
    PF00058. Ldl_recept_b. 2 hits.
    PF06119. NIDO. 1 hit.
    PF00086. Thyroglobulin_1. 2 hits.
    [Graphical view ]
    SMARTi SM00181. EGF. 3 hits.
    SM00179. EGF_CA. 2 hits.
    SM00682. G2F. 1 hit.
    SM00135. LY. 4 hits.
    SM00539. NIDO. 1 hit.
    SM00211. TY. 2 hits.
    [Graphical view ]
    SUPFAMi SSF54511. SSF54511. 1 hit.
    SSF57184. SSF57184. 2 hits.
    SSF57610. SSF57610. 2 hits.
    PROSITEi PS00010. ASX_HYDROXYL. 3 hits.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 5 hits.
    PS01187. EGF_CA. 2 hits.
    PS51120. LDLRB. 4 hits.
    PS51220. NIDO. 1 hit.
    PS50993. NIDOGEN_G2. 1 hit.
    PS00484. THYROGLOBULIN_1_1. 2 hits.
    PS51162. THYROGLOBULIN_1_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Entactin-2: a new member of basement membrane protein with high homology to entactin/nidogen."
      Kimura N., Toyoshima T., Kojima T., Shimane M.
      Exp. Cell Res. 241:36-45(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins."
      Talts J.F., Andac Z., Goehring W., Brancaccio A., Timpl R.
      EMBO J. 18:863-870(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LAMA2.
    5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-681.

    Entry informationi

    Entry nameiNID2_MOUSE
    AccessioniPrimary (citable) accession number: O88322
    Secondary accession number(s): Q7TQF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3