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Protein

Neurotensin receptor type 1

Gene

Ntsr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotensin receptor type 1
Short name:
NT-R-1
Short name:
NTR1
Gene namesi
Name:Ntsr1
Synonyms:Ntsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97386. Ntsr1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Membrane raft By similarity

  • Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
Transmembranei68 – 88Helical; Name=1By similarityAdd BLAST21
Topological domaini89 – 102CytoplasmicBy similarityAdd BLAST14
Transmembranei103 – 122Helical; Name=2By similarityAdd BLAST20
Topological domaini123 – 142ExtracellularBy similarityAdd BLAST20
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 205Helical; Name=4By similarityAdd BLAST21
Topological domaini206 – 234ExtracellularBy similarityAdd BLAST29
Transmembranei235 – 259Helical; Name=5By similarityAdd BLAST25
Topological domaini260 – 308CytoplasmicBy similarityAdd BLAST49
Transmembranei309 – 330Helical; Name=6By similarityAdd BLAST22
Topological domaini331 – 348ExtracellularBy similarityAdd BLAST18
Transmembranei349 – 369Helical; Name=7By similarityAdd BLAST21
Topological domaini370 – 424CytoplasmicBy similarityAdd BLAST55

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3570.
GuidetoPHARMACOLOGYi309.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699471 – 424Neurotensin receptor type 1Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi42N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi141 ↔ 224PROSITE-ProRule annotation
Glycosylationi211N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi386S-palmitoyl cysteineBy similarity1
Lipidationi388S-palmitoyl cysteineBy similarity1

Post-translational modificationi

N-glycosylated.By similarity
Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiO88319.
PRIDEiO88319.

PTM databases

iPTMnetiO88319.
PhosphoSitePlusiO88319.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027568.
CleanExiMM_NTSR1.
ExpressionAtlasiO88319. baseline and differential.
GenevisibleiO88319. MM.

Interactioni

Subunit structurei

Interacts (palmitoylated form) with GNA11.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029084.

Chemistry databases

BindingDBiO88319.

Structurei

3D structure databases

ProteinModelPortaliO88319.
SMRiO88319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni326 – 349Neurotensin bindingBy similarityAdd BLAST24

Domaini

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiO88319.
KOiK04211.
OMAiVPMLFTM.
OrthoDBiEOG091G0Q6E.
PhylomeDBiO88319.
TreeFamiTF337167.

Family and domain databases

CDDicd15355. 7tmA_NTSR1. 1 hit.
InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O88319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLNSSVQQG APSEPGAQPF PHPQFGLETM LLALSLSNGS GNSSESILEP
60 70 80 90 100
NSNLDVNTDI YSKVLVTAVY LALFVVGTVG NSVTAFTLAR KKSLQSLQST
110 120 130 140 150
VHYHLGSLAL SDLLILLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA
160 170 180 190 200
CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA
210 220 230 240 250
VPMLFTMGLQ NRSADGQHPG GLVCTPTVDT ATVKVVIQVN TFMSFLFPML
260 270 280 290 300
IISILNTVIA NKLTVMVHQA AEQGRGVCTV GTHNSLEHST FNMSIEPGRV
310 320 330 340 350
QALRHGVLVL RAVVIAFVVC WLPYHVRRLM FCYISDEQWT TFLFDFYHYF
360 370 380 390 400
YMLTNALFYV SSAINPILYN LVSANFRQVF LSTLACLCPG WRRRRKKRPT
410 420
FSRKPNSMSS NHAFSTSATR ETLY
Length:424
Mass (Da):47,217
Last modified:November 1, 1998 - v1
Checksum:i8E9A723171A48711
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017027 mRNA. Translation: BAA33013.1.
CCDSiCCDS17177.1.
RefSeqiNP_061236.1. NM_018766.2.
UniGeneiMm.301712.

Genome annotation databases

EnsembliENSMUST00000029084; ENSMUSP00000029084; ENSMUSG00000027568.
ENSMUST00000170448; ENSMUSP00000127548; ENSMUSG00000027568.
GeneIDi18216.
KEGGimmu:18216.
UCSCiuc008ojg.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiNTR1_MOUSE
AccessioniPrimary (citable) accession number: O88319
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: August 30, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families