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Protein

Neurotensin receptor type 1

Gene

Ntsr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.By similarity

GO - Molecular functioni

  • G-protein coupled neurotensin receptor activity Source: UniProtKB
  • neuropeptide receptor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotensin receptor type 1
Short name:
NT-R-1
Short name:
NTR1
Gene namesi
Name:Ntsr1
Synonyms:Ntsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97386. Ntsr1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Membrane raft By similarity

  • Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
Transmembranei68 – 88Helical; Name=1By similarityAdd BLAST21
Topological domaini89 – 102CytoplasmicBy similarityAdd BLAST14
Transmembranei103 – 122Helical; Name=2By similarityAdd BLAST20
Topological domaini123 – 142ExtracellularBy similarityAdd BLAST20
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 205Helical; Name=4By similarityAdd BLAST21
Topological domaini206 – 234ExtracellularBy similarityAdd BLAST29
Transmembranei235 – 259Helical; Name=5By similarityAdd BLAST25
Topological domaini260 – 308CytoplasmicBy similarityAdd BLAST49
Transmembranei309 – 330Helical; Name=6By similarityAdd BLAST22
Topological domaini331 – 348ExtracellularBy similarityAdd BLAST18
Transmembranei349 – 369Helical; Name=7By similarityAdd BLAST21
Topological domaini370 – 424CytoplasmicBy similarityAdd BLAST55

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3570.
GuidetoPHARMACOLOGYi309.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699471 – 424Neurotensin receptor type 1Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...)Sequence analysis1
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Glycosylationi42N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi141 ↔ 224PROSITE-ProRule annotation
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1
Lipidationi386S-palmitoyl cysteineBy similarity1
Lipidationi388S-palmitoyl cysteineBy similarity1

Post-translational modificationi

N-gycosylated.By similarity
Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiO88319.
PRIDEiO88319.

PTM databases

iPTMnetiO88319.
PhosphoSitePlusiO88319.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027568.
CleanExiMM_NTSR1.
ExpressionAtlasiO88319. baseline and differential.
GenevisibleiO88319. MM.

Interactioni

Subunit structurei

Interacts (palmitoylated form) with GNA11.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029084.

Chemistry databases

BindingDBiO88319.

Structurei

3D structure databases

ProteinModelPortaliO88319.
SMRiO88319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni326 – 349Neurotensin bindingBy similarityAdd BLAST24

Domaini

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiO88319.
KOiK04211.
OMAiTVMVRQA.
OrthoDBiEOG091G0Q6E.
PhylomeDBiO88319.
TreeFamiTF337167.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLNSSVQQG APSEPGAQPF PHPQFGLETM LLALSLSNGS GNSSESILEP
60 70 80 90 100
NSNLDVNTDI YSKVLVTAVY LALFVVGTVG NSVTAFTLAR KKSLQSLQST
110 120 130 140 150
VHYHLGSLAL SDLLILLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA
160 170 180 190 200
CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA
210 220 230 240 250
VPMLFTMGLQ NRSADGQHPG GLVCTPTVDT ATVKVVIQVN TFMSFLFPML
260 270 280 290 300
IISILNTVIA NKLTVMVHQA AEQGRGVCTV GTHNSLEHST FNMSIEPGRV
310 320 330 340 350
QALRHGVLVL RAVVIAFVVC WLPYHVRRLM FCYISDEQWT TFLFDFYHYF
360 370 380 390 400
YMLTNALFYV SSAINPILYN LVSANFRQVF LSTLACLCPG WRRRRKKRPT
410 420
FSRKPNSMSS NHAFSTSATR ETLY
Length:424
Mass (Da):47,217
Last modified:November 1, 1998 - v1
Checksum:i8E9A723171A48711
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017027 mRNA. Translation: BAA33013.1.
CCDSiCCDS17177.1.
RefSeqiNP_061236.1. NM_018766.2.
UniGeneiMm.301712.

Genome annotation databases

EnsembliENSMUST00000029084; ENSMUSP00000029084; ENSMUSG00000027568.
ENSMUST00000170448; ENSMUSP00000127548; ENSMUSG00000027568.
GeneIDi18216.
KEGGimmu:18216.
UCSCiuc008ojg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017027 mRNA. Translation: BAA33013.1.
CCDSiCCDS17177.1.
RefSeqiNP_061236.1. NM_018766.2.
UniGeneiMm.301712.

3D structure databases

ProteinModelPortaliO88319.
SMRiO88319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029084.

Chemistry databases

BindingDBiO88319.
ChEMBLiCHEMBL3570.
GuidetoPHARMACOLOGYi309.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiO88319.
PhosphoSitePlusiO88319.

Proteomic databases

PaxDbiO88319.
PRIDEiO88319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029084; ENSMUSP00000029084; ENSMUSG00000027568.
ENSMUST00000170448; ENSMUSP00000127548; ENSMUSG00000027568.
GeneIDi18216.
KEGGimmu:18216.
UCSCiuc008ojg.1. mouse.

Organism-specific databases

CTDi4923.
MGIiMGI:97386. Ntsr1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiO88319.
KOiK04211.
OMAiTVMVRQA.
OrthoDBiEOG091G0Q6E.
PhylomeDBiO88319.
TreeFamiTF337167.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiO88319.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027568.
CleanExiMM_NTSR1.
ExpressionAtlasiO88319. baseline and differential.
GenevisibleiO88319. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTR1_MOUSE
AccessioniPrimary (citable) accession number: O88319
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.