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O88312 (AGR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anterior gradient protein 2 homolog
Short name=AG-2
Short name=mAG-2
Alternative name(s):
Protein Gob-4
Secreted cement gland protein XAG-2 homolog
Gene names
Name:Agr2
Synonyms:Gob4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth By similarity. Ref.6

Subunit structure

Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked By similarity.

Subcellular location

Secreted By similarity. Endoplasmic reticulum Ref.6.

Tissue specificity

Expressed in lung, skeletal muscle, testis, liver, stomach, colon, small intestine, the goblet cells of the intestine and the mucuous neck cells of the stomach. Ref.1 Ref.2

Developmental stage

Expressed in embryo at 15 dpc onwards.

Disruption phenotype

Mice are viable but display altered production of mucus with loss of production of MUC2 despite expression of its mRNA. They also display an increase in mast cells in the intestine, an increased expression of inflamation-specific genes, and frequent rectal prolapse. This is associated with a higher susceptibility to colitis. Ref.6

Sequence similarities

Belongs to the AGR family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Secreted
   DiseaseProto-oncogene
   DomainSignal
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processmucus secretion

Inferred from mutant phenotype Ref.6. Source: UniProtKB

   Cellular componentendoplasmic reticulum

Inferred from direct assay Ref.6. Source: UniProtKB

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 175155Anterior gradient protein 2 homolog
PRO_0000001038

Sequences

Sequence LengthMass (Da)Tools
O88312 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: ACC3CFE429B668CA

FASTA17519,920
        10         20         30         40         50         60 
MEKFSVSAIL LLVAISGTLA KDTTVKSGAK KDPKDSRPKL PQTLSRGWGD QLIWTQTYEE 

        70         80         90        100        110        120 
ALYRSKTSNR PLMVIHHLDE CPHSQALKKV FAEHKEIQKL AEQFVLLNLV YETTDKHLSP 

       130        140        150        160        170 
DGQYVPRIVF VDPSLTVRAD ITGRYSNRLY AYEPSDTALL YDNMKKALKL LKTEL

« Hide

References

« Hide 'large scale' references
[1]"hAG-2, the human homologue of the Xenopus laevis cement gland gene XAG-2, is coexpressed with estrogen receptor in breast cancer cell lines."
Thompson D.A., Weigel R.J.
Biochem. Biophys. Res. Commun. 251:111-116(1998) [PubMed: 9790916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Swiss Webster.
Tissue: Embryo.
[2]"Cloning of a gene, gob-4, which is expressed in intestinal goblet cells in mice."
Komiya T., Hirohashi S.
Biochim. Biophys. Acta 1444:434-438(1999) [PubMed: 10095068] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Intestine.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Pancreas.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
[6]"The protein disulfide isomerase AGR2 is essential for production of intestinal mucus."
Park S.-W., Zhen G., Verhaeghe C., Nakagami Y., Nguyenvu L.T., Barczak A.J., Killeen N., Erle D.J.
Proc. Natl. Acad. Sci. U.S.A. 106:6950-6955(2009) [PubMed: 19359471] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF044262 mRNA. Translation: AAC72705.1.
AB016592 mRNA. Translation: BAA32044.1.
AK007677 mRNA. Translation: BAB25181.1.
CT030196 Genomic DNA. Translation: CAM19620.1.
BC013334 mRNA. Translation: AAH13334.1.
IPIIPI00129857.
RefSeqNP_035913.1. NM_011783.2.
UniGeneMm.7244.

3D structure databases

ProteinModelPortalO88312.
SMRO88312. Positions 44-174.
ModBaseSearch...

Protein-protein interaction databases

IntActO88312. 1 interaction.
STRINGO88312.

Proteomic databases

PRIDEO88312.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020898; ENSMUSP00000020898; ENSMUSG00000020581.
GeneID23795.
KEGGmmu:23795.
UCSCuc007njm.1. mouse.

Organism-specific databases

CTD10551.
MGIMGI:1344405. Agr2.

Phylogenomic databases

GeneTreeENSGT00530000063273.
HOGENOMHBG506972.
HOVERGENHBG006516.
InParanoidO88312.
OMAAYEPSDI.
OrthoDBEOG4KH2W7.
PhylomeDBO88312.

Gene expression databases

ArrayExpressO88312.
BgeeO88312.
CleanExMM_AGR2.
GenevestigatorO88312.
GermOnlineENSMUSG00000020581. Mus musculus.

Family and domain databases

InterProIPR012336. Thioredoxin-like_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
ProtoNetSearch...

Other

NextBio303407.
SOURCESearch...

Entry information

Entry nameAGR2_MOUSE
AccessionPrimary (citable) accession number: O88312
Secondary accession number(s): A2CGA2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 1, 1998
Last modified: September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents