Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Intelectin-1a

Gene

Itln1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed:26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris. Does not bind mammalian glycans. Probably plays a role in the defense system against microorganisms. May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi86Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi87Calcium 2By similarity1
Metal bindingi89Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi92Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi97Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi98Calcium 2By similarity1
Metal bindingi133Calcium 1By similarity1
Metal bindingi260Calcium 3By similarity1
Metal bindingi262Calcium 3By similarity1
Metal bindingi274Calcium 3By similarity1
Binding sitei274CarbohydrateBy similarity1
Metal bindingi282Calcium 1By similarity1

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • carbohydrate binding Source: MGI
  • oligosaccharide binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandCalcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Intelectin-1a
Alternative name(s):
Galactofuranose-binding lectin
Intestinal lactoferrin receptor1 Publication1 Publication
Gene namesi
Name:Itln1
Synonyms:Intl, Itln, Itln1a, Itlna
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1333831. Itln1.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity
  • Secreted By similarity

  • Note: Enriched in lipid rafts.1 Publication

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • brush border membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • membrane raft Source: UniProtKB
  • receptor complex Source: MGI

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000000914519 – 298Intelectin-1aAdd BLAST280
PropeptideiPRO_0000009146299 – 313Sequence analysisAdd BLAST15

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 70By similarity
Disulfide bondi94 ↔ 280By similarity
Disulfide bondi199 ↔ 259By similarity
Disulfide bondi251 ↔ 265By similarity
Lipidationi298GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiO88310.
PaxDbiO88310.
PRIDEiO88310.

PTM databases

iPTMnetiO88310.
PhosphoSitePlusiO88310.

Expressioni

Tissue specificityi

Expressed in small intestinal Paneth cells in uninfected mice. Expression also detected in various other tissues including stomach, kidney, ovary and brain.5 Publications

Developmental stagei

In the embryo, levels are highest at day 7, decrease by mid-embryogenesis at day 11 and increase again in late embryogenesis at day 17.1 Publication

Inductioni

By infection with the helminth parasite N.brasiliensis.1 Publication

Gene expression databases

BgeeiENSMUSG00000038209.
CleanExiMM_ITLN1.
ExpressionAtlasiO88310. baseline and differential.
GenevisibleiO88310. MM.

Interactioni

Subunit structurei

Monomer (PubMed:17621593). May interact with LTF (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043837.

Structurei

3D structure databases

ProteinModelPortaliO88310.
SMRiO88310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 251Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST220

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 263Carbohydrate bindingBy similarity2

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGEB. Eukaryota.
ENOG4110R6N. LUCA.
GeneTreeiENSGT00390000008567.
HOGENOMiHOG000026788.
HOVERGENiHBG052157.
InParanoidiO88310.
KOiK17527.
OMAiYIARSCK.
OrthoDBiEOG09040EBV.
PhylomeDBiO88310.
TreeFamiTF328530.

Family and domain databases

Gene3Di3.90.215.10. 1 hit.
InterProiView protein in InterPro
IPR014716. Fibrinogen_a/b/g_C_1.
IPR002181. Fibrinogen_a/b/g_C_dom.
PfamiView protein in Pfam
PF00147. Fibrinogen_C. 1 hit.
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiView protein in PROSITE
PS51406. FIBRINOGEN_C_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQLGFLLFI MVATRGCSAA EENLDTNRWG NSFFSSLPRS CKEIKQEHTK
60 70 80 90 100
AQDGLYFLRT KNGVIYQTFC DMTTAGGGWT LVASVHENNM RGKCTVGDRW
110 120 130 140 150
SSQQGNRADY PEGDGNWANY NTFGSAEAAT SDDYKNPGYF DIQAENLGIW
160 170 180 190 200
HVPNKSPLHN WRKSSLLRYR TFTGFLQHLG HNLFGLYKKY PVKYGEGKCW
210 220 230 240 250
TDNGPALPVV YDFGDARKTA SYYSPSGQRE FTAGYVQFRV FNNERAASAL
260 270 280 290 300
CAGVRVTGCN TEHHCIGGGG FFPEGNPVQC GDFASFDWDG YGTHNGYSSS
310
RKITEAAVLL FYR
Length:313
Mass (Da):34,953
Last modified:November 1, 1998 - v1
Checksum:iD2FA447D3D8547A4
GO

Mass spectrometryi

Molecular mass is 34000 Da from positions 20 - 298. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016496 mRNA. Translation: BAA31992.1.
AY157363 mRNA. Translation: AAO17802.1.
AY157364 mRNA. Translation: AAO17803.1.
AY619693 mRNA. Translation: AAU88049.1.
AK007447 mRNA. Translation: BAB25043.1.
CCDSiCCDS35777.1.
PIRiJE0328.
RefSeqiNP_034714.1. NM_010584.3.
UniGeneiMm.247426.

Genome annotation databases

EnsembliENSMUST00000043094; ENSMUSP00000043837; ENSMUSG00000038209.
GeneIDi16429.
KEGGimmu:16429.
UCSCiuc007doq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016496 mRNA. Translation: BAA31992.1.
AY157363 mRNA. Translation: AAO17802.1.
AY157364 mRNA. Translation: AAO17803.1.
AY619693 mRNA. Translation: AAU88049.1.
AK007447 mRNA. Translation: BAB25043.1.
CCDSiCCDS35777.1.
PIRiJE0328.
RefSeqiNP_034714.1. NM_010584.3.
UniGeneiMm.247426.

3D structure databases

ProteinModelPortaliO88310.
SMRiO88310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043837.

PTM databases

iPTMnetiO88310.
PhosphoSitePlusiO88310.

Proteomic databases

MaxQBiO88310.
PaxDbiO88310.
PRIDEiO88310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043094; ENSMUSP00000043837; ENSMUSG00000038209.
GeneIDi16429.
KEGGimmu:16429.
UCSCiuc007doq.2. mouse.

Organism-specific databases

CTDi55600.
MGIiMGI:1333831. Itln1.

Phylogenomic databases

eggNOGiENOG410IGEB. Eukaryota.
ENOG4110R6N. LUCA.
GeneTreeiENSGT00390000008567.
HOGENOMiHOG000026788.
HOVERGENiHBG052157.
InParanoidiO88310.
KOiK17527.
OMAiYIARSCK.
OrthoDBiEOG09040EBV.
PhylomeDBiO88310.
TreeFamiTF328530.

Enzyme and pathway databases

ReactomeiR-MMU-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiO88310.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038209.
CleanExiMM_ITLN1.
ExpressionAtlasiO88310. baseline and differential.
GenevisibleiO88310. MM.

Family and domain databases

Gene3Di3.90.215.10. 1 hit.
InterProiView protein in InterPro
IPR014716. Fibrinogen_a/b/g_C_1.
IPR002181. Fibrinogen_a/b/g_C_dom.
PfamiView protein in Pfam
PF00147. Fibrinogen_C. 1 hit.
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiView protein in PROSITE
PS51406. FIBRINOGEN_C_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiITL1A_MOUSE
AccessioniPrimary (citable) accession number: O88310
Secondary accession number(s): Q5IWS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1998
Last modified: February 15, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Constitutive expression does not lead to enhanced immunity to N.brasiliensis or M.tuberculosis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.