Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - O88286 (WIZ_MOUSE)

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein Wiz

Gene

Wiz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved in EHMT1-EHMT2 heterodimer formation and stabilization.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri308 – 330C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri345 – 367C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri454 – 477C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri734 – 756C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri802 – 824C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri903 – 925C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1076 – 1098C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1260 – 1282C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1430 – 1452C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1629 – 1655C2H2-type 10PROSITE-ProRule annotationAdd BLAST27

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro
  • SET domain binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  • positive regulation of nuclear cell cycle DNA replication Source: MGI
  • protein heterotrimerization Source: UniProtKB
  • protein stabilization Source: UniProtKB
Complete GO annotation...

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wiz
Alternative name(s):
Widely-interspaced zinc finger-containing protein
Gene namesi
Name:Wiz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1332638. Wiz.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • midbody Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1631C → A: Impairs interaction with EHMT1 and EHMT2. 1 Publication1
Mutagenesisi1651H → A: Impairs interaction with EHMT1 and EHMT2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002860551 – 1684Protein WizAdd BLAST1684

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki972Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1021Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1029PhosphoserineBy similarity1
Modified residuei1031PhosphothreonineBy similarity1
Modified residuei1039PhosphoserineCombined sources1
Modified residuei1045PhosphoserineCombined sources1
Modified residuei1049PhosphothreonineCombined sources1
Modified residuei1050PhosphoserineCombined sources1
Modified residuei1058PhosphoserineBy similarity1
Modified residuei1112PhosphoserineBy similarity1
Modified residuei1139PhosphoserineBy similarity1
Cross-linki1141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1145Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1155PhosphoserineBy similarity1
Modified residuei1160PhosphoserineBy similarity1
Modified residuei1167PhosphoserineBy similarity1
Modified residuei1179PhosphoserineCombined sources1
Modified residuei1184PhosphoserineCombined sources1
Modified residuei1195N6,N6,N6-trimethyllysine; by EHMT2; alternateBy similarity1
Modified residuei1195N6,N6-dimethyllysine; by EHMT2; alternateBy similarity1
Cross-linki1210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1296PhosphoserineBy similarity1
Cross-linki1315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1342PhosphoserineBy similarity1
Modified residuei1347PhosphoserineBy similarity1
Cross-linki1376Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1389Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1403Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1405Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1415Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1481Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1510Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1550PhosphoserineBy similarity1
Cross-linki1556Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki1556Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO88286.
MaxQBiO88286.
PaxDbiO88286.
PeptideAtlasiO88286.
PRIDEiO88286.

PTM databases

iPTMnetiO88286.
PhosphoSitePlusiO88286.

Expressioni

Tissue specificityi

According to PubMed:9795207, isoform L and isoform S are brain-specific. According to PubMed:16702210, isoform S is ubiquitously expressed.2 Publications

Developmental stagei

Isoform L and isoform S are expressed in brain from E14 to adulthood, with highest levels in the perinatal period. At embryonic stages, isoform L seems to be excluded from cerebellum.1 Publication

Gene expression databases

BgeeiENSMUSG00000024050.
CleanExiMM_WIZ.
ExpressionAtlasiO88286. baseline and differential.
GenevisibleiO88286. MM.

Interactioni

Subunit structurei

Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 (By similarity). Interacts with EHMT1, EHMT2, CTBP1 and CTBP2.By similarity1 Publication

GO - Molecular functioni

  • SET domain binding Source: UniProtKB
Complete GO annotation...

Protein-protein interaction databases

BioGridi204563. 28 interactors.
IntActiO88286. 26 interactors.
MINTiMINT-4105440.
STRINGi10090.ENSMUSP00000069443.

Structurei

3D structure databases

ProteinModelPortaliO88286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1063 – 1067Interaction with CTBP1 and CTBP2 11 Publication5
Regioni1247 – 1251Interaction with CTBP1 and CTBP2 21 Publication5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi299 – 304Poly-Glu6
Compositional biasi601 – 608Poly-Glu8
Compositional biasi658 – 663Poly-Gln6
Compositional biasi786 – 790Poly-Lys5
Compositional biasi1174 – 1177Poly-Pro4

Domaini

The C2H2-type zinc finger 10 mediates interaction with EHMT1 and EHMT2.

Sequence similaritiesi

Belongs to the krueppel C2H2-type zinc-finger protein family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri308 – 330C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri345 – 367C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri454 – 477C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri734 – 756C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri802 – 824C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri903 – 925C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1076 – 1098C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1260 – 1282C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1430 – 1452C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1629 – 1655C2H2-type 10PROSITE-ProRule annotationAdd BLAST27

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063587.
HOGENOMiHOG000154859.
HOVERGENiHBG108678.
InParanoidiO88286.
OMAiHFGLECS.
PhylomeDBiO88286.
TreeFamiTF333705.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR013087. Znf_C2H2_type.
IPR013083. Znf_RING/FYVE/PHD.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 11 hits.
SUPFAMiSSF57667. SSF57667. 5 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform L (identifier: O88286-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGLLAGGLA APDHPRGPAP REDIESGAEA AEGEGDIFPS SHYLPITKEG 
        60         70         80         90        100
PRDILDGRSG ISDGQPHPGL SEALPRATSA THRISSCYWD GDSLDFQPGS 
       110        120        130        140        150
PPPHLLGPFP ASLDVQGSWE HPMVQEAREG TPSEQRFKDS VIVRTMKPYA 
       160        170        180        190        200
KLKGSRKFLH HQGEVKFLEK YSPSHHKFDW LQDTDEQGPL KDTGLHLDLP 
       210        220        230        240        250
AQPPTVTSFR RVIVPVDNTP KTLDMEVMGT REDLEDFGQV AQPSEWGLHT 
       260        270        280        290        300
SASEVATQTW TVNSEASVER LQPLLSPVQT GPYLCELLQE VAGGVDSNEE 
       310        320        330        340        350
EEEEPAVFPC IECSIYFKHK EHLLEHMSQH RRAPGQEPPA DLAPLACSEC 
       360        370        380        390        400
GWAFTEPTAL EQHWQLHQAS REKIIEEIQK LKQFPGDEGR EARLQCSKCV 
       410        420        430        440        450
FGTNSSRAFM QHAKLHVRGS LPSRQATEPF RGGSPVLDVS TLVYPSYGDS 
       460        470        480        490        500
SGLNTCVHCG FTAPSKSLLR EHTRLVHAHH AHWEEVGEAF EDLTSQPCTS 
       510        520        530        540        550
QDAYTHSPDT ATVDYFSKSE PLLASVWQEN PSGYDPDLAF GPDYQQPGMR 
       560        570        580        590        600
NFPLLNSGQQ SLGKLAFPSP MASASYSIQR NRNKSTVHLQ RMEDKSHLWS 
       610        620        630        640        650
EEEEEEDEDV VLTSERDFTP ENGAFPPLAI PSLIPQPALE LKQTFQDALQ 
       660        670        680        690        700
AVDASETQQQ QLQGMVPIVL MAKLRPQVIA ATTRASPQLP PEEPELRSTH 
       710        720        730        740        750
PLDFLLLDAP LGGSLGLNTL LEGDPAMALK HEERKCPYCP DRFHYGIGLA 
       760        770        780        790        800
NHVRGHLNRV GVSYNVRHFI SAEEVKAIER RFSFQKKKKK VANFDPGTFS 
       810        820        830        840        850
LMRCDFCGAG FDTRAGLSSH ARAHLRDFGI TNWELTISPI NILQELLATS 
       860        870        880        890        900
AAELPPSPLG REPGGPPRSF LTSRRPRLPL TMPFPPTWAE DPGPIYGDAQ 
       910        920        930        940        950
SLTTCEVCGA CFETRKGLSS HARSHLRQLG VAESESSGAP IDLLYELVKQ 
       960        970        980        990       1000
KGLPDAPLGL TPSLTKKSNS PKEFLAGAAR PGLLTLAKPM DAPAVNKAIK 
      1010       1020       1030       1040       1050
SPPGFSAKGL THPSSSPLLK KAPLTLAGSP TPKNPEDKSP QLSLSPRPTS 
      1060       1070       1080       1090       1100
PKAQWPQSED EGPLNLTSGP EPTRDIRCEF CGEFFENRKG LSSHARSHLR 
      1110       1120       1130       1140       1150
QMGVTEWYVN GSPIDTLREI LKRRTQSRPG GHLHPPGPSP KALAKVLSTG 
      1160       1170       1180       1190       1200
GPGSSLEARS PSDLHISPLT KKLPPPPGSP LGHSPTASPP PTARKMFSGL 
      1210       1220       1230       1240       1250
ATPSLPKKLK PEHMRVEIKR EMLPGTLHGE PHPSEGPWGT PREDMAPLNL 
      1260       1270       1280       1290       1300
SARAEPVRDI RCEFCGEFFE NRKGLSSHAR SHLRQMGVTE WSVNGSPIDT 
      1310       1320       1330       1340       1350
LREILKKKSK LCLIKKEPPA GDLAPALTED GSPTAAPGAL HSPLPLSPLA 
      1360       1370       1380       1390       1400
SRPGKPGAGP TQVPRELSLS PITGSKPSAA SYLGPVATKR PLQEDRFLPA 
      1410       1420       1430       1440       1450
EVKAKTYIQT ELPFKAKTLH EKTSHSSTEA CCELCGLYFE NRKALASHAR 
      1460       1470       1480       1490       1500
AHLRQFGVTE WCVNGSPIET LSEWIKHRPQ KVGAYRSYIQ GGRPFTKKFR 
      1510       1520       1530       1540       1550
SAGHGRDSDK RPPLGLAPGG LSLVGRSAGG EPGLEAGRAA DSGERPLATS 
      1560       1570       1580       1590       1600
PPGTVKSEEH QRQNINKFER RQARPSDASA ARGGEEVNDL QQKLEEVRQP 
      1610       1620       1630       1640       1650
PPRVRPVPSL VPRPPQTSLV KFVGNIYTLK CRFCEVEFQG PLSIQEEWVR 
      1660       1670       1680 
HLQRHILEMN FSKADPPPEE PQAPQAQTAA VEAP
Length:1,684
Mass (Da):184,291
Last modified:May 1, 2007 - v2
Checksum:i67BA8AC25A97DDEB
GO
Isoform S (identifier: O88286-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     63-790: Missing.

Show »
Length:956
Mass (Da):103,003
Checksum:i4DDE4A816FC04D65
GO
Isoform 2 (identifier: O88286-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-759: Missing.
     760-789: VGVSYNVRHFISAEEVKAIERRFSFQKKKK → MAEVAFLMGSPILASAKPSPVPPPPPIGSA
     898-898: D → DGLCSEENTMVAMDLGSPLLPKKSLPVSGTLEQVASRLSSKVAAEVPHGSKQELPDLK

Note: No experimental confirmation available.
Show »
Length:982
Mass (Da):105,637
Checksum:i1304FDF9672338FC
GO
Isoform 3 (identifier: O88286-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     745-798: YGIGLANHVR...KKVANFDPGT → NGIVLRPPAA...AQILLLRHQL
     799-1684: Missing.

Note: No experimental confirmation available.
Show »
Length:798
Mass (Da):88,329
Checksum:iCD980D3BFAD521B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti492D → G in BAA32790 (PubMed:9795207).Curated1
Sequence conflicti868R → G in BAA32790 (PubMed:9795207).Curated1
Sequence conflicti868R → G in BAA32791 (PubMed:9795207).Curated1
Sequence conflicti1020K → N in AAH53035 (PubMed:16141072).Curated1
Sequence conflicti1052K → R in BAA32790 (PubMed:9795207).Curated1
Sequence conflicti1052K → R in BAA32791 (PubMed:9795207).Curated1
Sequence conflicti1055W → C in BAA32790 (PubMed:9795207).Curated1
Sequence conflicti1055W → C in BAA32791 (PubMed:9795207).Curated1
Sequence conflicti1252Missing in BAA32790 (PubMed:9795207).Curated1
Sequence conflicti1252Missing in BAA32791 (PubMed:9795207).Curated1

Polymorphismi

C57BL/6J and C57BR/cdJ mice specifically present an insertion of a type II early transposon in the opposite orientation into exon 6. This results in a strong reduction in the protein levels of isoform L.1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249531 – 759Missing in isoform 2. 1 PublicationAdd BLAST759
Alternative sequenceiVSP_02495463 – 790Missing in isoform S. 2 PublicationsAdd BLAST728
Alternative sequenceiVSP_024955745 – 798YGIGL…FDPGT → NGIVLRPPAARNVNGSTEKA ARAVREESRRGKRRNVAKTV TLIKAQILLLRHQL in isoform 3. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_024956760 – 789VGVSY…QKKKK → MAEVAFLMGSPILASAKPSP VPPPPPIGSA in isoform 2. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_024957799 – 1684Missing in isoform 3. 1 PublicationAdd BLAST886
Alternative sequenceiVSP_024958898D → DGLCSEENTMVAMDLGSPLL PKKSLPVSGTLEQVASRLSS KVAAEVPHGSKQELPDLK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012265 mRNA. Translation: BAA32790.1.
AB012266 mRNA. Translation: BAA32791.1.
AB255388 mRNA. Translation: BAE93139.1.
AK004274 mRNA. Translation: BAB23245.1.
AK133564 mRNA. Translation: BAE21728.1.
BC053035 mRNA. Translation: AAH53035.1.
CCDSiCCDS28617.2. [O88286-2]
PIRiT00247.
T00248.
RefSeqiNP_035847.2. NM_011717.4.
NP_997603.3. NM_212438.3. [O88286-2]
XP_017172890.1. XM_017317401.1. [O88286-2]
UniGeneiMm.274948.

Genome annotation databases

EnsembliENSMUST00000064694; ENSMUSP00000069443; ENSMUSG00000024050. [O88286-2]
GeneIDi22404.
KEGGimmu:22404.
UCSCiuc008bwn.2. mouse. [O88286-2]
uc008bwq.2. mouse. [O88286-3]
uc008bwt.2. mouse. [O88286-4]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiWIZ_MOUSE
AccessioniPrimary (citable) accession number: O88286
Secondary accession number(s): O88287
, Q1XG03, Q3UZX7, Q7TSJ4, Q9CT89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: June 7, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families