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Protein

Slit homolog 3 protein

Gene

Slit3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.

GO - Molecular functioni

GO - Biological processi

  • axon extension involved in axon guidance Source: GO_Central
  • brain development Source: RGD
  • regulation of axonogenesis Source: GO_Central
  • Roundabout signaling pathway Source: GO_Central
  • spinal cord development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Slit homolog 3 protein
Short name:
Slit-3
Alternative name(s):
Multiple epidermal growth factor-like domains protein 5
Short name:
Multiple EGF-like domains protein 5
Gene namesi
Name:Slit3
Synonyms:Megf5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69311. Slit3.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: GO_Central
  • membrane Source: RGD
  • proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 15231490Slit homolog 3 proteinPRO_0000007734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence analysis
Disulfide bondi284 ↔ 293By similarity
Disulfide bondi441 ↔ 464By similarity
Disulfide bondi443 ↔ 485By similarity
Disulfide bondi505 ↔ 511By similarity
Disulfide bondi509 ↔ 518By similarity
Glycosylationi563 – 5631N-linked (GlcNAc...)Sequence analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence analysis
Disulfide bondi667 ↔ 690By similarity
Disulfide bondi669 ↔ 711By similarity
Glycosylationi784 – 7841N-linked (GlcNAc...)Sequence analysis
Glycosylationi792 – 7921N-linked (GlcNAc...)Sequence analysis
Glycosylationi797 – 7971N-linked (GlcNAc...)Sequence analysis
Disulfide bondi920 ↔ 931By similarity
Disulfide bondi925 ↔ 941By similarity
Glycosylationi928 – 9281N-linked (GlcNAc...)Sequence analysis
Disulfide bondi943 ↔ 952By similarity
Disulfide bondi959 ↔ 970By similarity
Disulfide bondi964 ↔ 982By similarity
Disulfide bondi984 ↔ 993By similarity
Disulfide bondi1000 ↔ 1011By similarity
Disulfide bondi1005 ↔ 1020By similarity
Disulfide bondi1022 ↔ 1031By similarity
Glycosylationi1025 – 10251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi1038 ↔ 1051By similarity
Disulfide bondi1045 ↔ 1060By similarity
Disulfide bondi1062 ↔ 1071By similarity
Disulfide bondi1078 ↔ 1089By similarity
Disulfide bondi1083 ↔ 1098By similarity
Disulfide bondi1100 ↔ 1109By similarity
Disulfide bondi1123 ↔ 1134By similarity
Disulfide bondi1128 ↔ 1143By similarity
Disulfide bondi1145 ↔ 1154By similarity
Glycosylationi1181 – 11811N-linked (GlcNAc...)Sequence analysis
Glycosylationi1247 – 12471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi1305 ↔ 1332By similarity
Disulfide bondi1355 ↔ 1364By similarity
Disulfide bondi1372 ↔ 1382By similarity
Disulfide bondi1377 ↔ 1391By similarity
Disulfide bondi1393 ↔ 1402By similarity
Glycosylationi1406 – 14061N-linked (GlcNAc...)Sequence analysis
Disulfide bondi1412 ↔ 1422By similarity
Disulfide bondi1417 ↔ 1432By similarity
Disulfide bondi1434 ↔ 1443By similarity
Disulfide bondi1449 ↔ 1487By similarity
Disulfide bondi1467 ↔ 1501By similarity
Disulfide bondi1478 ↔ 1517By similarity
Disulfide bondi1482 ↔ 1519By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88280.
PRIDEiO88280.

Expressioni

Developmental stagei

Detected at E20, and between P0 and P5 in olfactory mitral cells. Detected between E18 and E20, between P0 and P10, and in adult in anterior olfactor, hypothalamus ventromedial nuclei. Detected at E15 in cortex marginal zone. Detected between E15 and E20, between P0 and P10, and in adult in cortex entorhinal and periform region, hippocampal regions, basal telencephalon bed stria terminalis nuclei. Detected at E20, between P0 and P10, and in adult in cortex induseum griseum and tenia tecta, and basal telencephalon olfactory tubercle. Detected between P0 and P10, and in adult in ventral thalamus zona incerta. Detected between P5 and P10, and in adult in ventral thalamus reticular nuclei. Detected between P0 and P10, and in adult in dorsal thalamus regions.1 Publication

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiO88280. 1 interaction.
STRINGi10116.ENSRNOP00000009714.

Structurei

3D structure databases

ProteinModelPortaliO88280.
SMRiO88280. Positions 505-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 6128LRRNTAdd
BLAST
Repeati62 – 8322LRR 1Add
BLAST
Repeati86 – 10722LRR 2Add
BLAST
Repeati110 – 13122LRR 3Add
BLAST
Repeati134 – 15522LRR 4Add
BLAST
Repeati158 – 17922LRR 5Add
BLAST
Repeati182 – 20322LRR 6Add
BLAST
Domaini215 – 26551LRRCT 1Add
BLAST
Domaini271 – 30737LRRNT 2Add
BLAST
Repeati308 – 32922LRR 7Add
BLAST
Repeati332 – 35322LRR 8Add
BLAST
Repeati356 – 37722LRR 9Add
BLAST
Repeati380 – 40122LRR 10Add
BLAST
Repeati404 – 42522LRR 11Add
BLAST
Domaini437 – 48751LRRCT 2Add
BLAST
Domaini496 – 53237LRRNT 3Add
BLAST
Repeati533 – 55422LRR 12Add
BLAST
Repeati558 – 57922LRR 13Add
BLAST
Repeati582 – 60322LRR 14Add
BLAST
Repeati606 – 62722LRR 15Add
BLAST
Repeati630 – 65122LRR 16Add
BLAST
Domaini663 – 71351LRRCT 3Add
BLAST
Domaini716 – 75237LRRNT 4Add
BLAST
Repeati753 – 77422LRR 17Add
BLAST
Repeati776 – 79722LRR 18Add
BLAST
Repeati800 – 82122LRR 19Add
BLAST
Repeati824 – 84522LRR 20Add
BLAST
Domaini857 – 90751LRRCT 4Add
BLAST
Domaini918 – 95336EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini955 – 99440EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini996 – 103237EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini1034 – 107239EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini1074 – 111037EGF-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini1119 – 115537EGF-like 6PROSITE-ProRule annotationAdd
BLAST
Domaini1158 – 1332175Laminin G-likePROSITE-ProRule annotationAdd
BLAST
Domaini1340 – 136526EGF-like 7PROSITE-ProRule annotationAdd
BLAST
Domaini1368 – 140336EGF-like 8PROSITE-ProRule annotationAdd
BLAST
Domaini1408 – 144437EGF-like 9PROSITE-ProRule annotationAdd
BLAST
Domaini1449 – 152375CTCKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 9 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.PROSITE-ProRule annotation
Contains 20 LRR (leucine-rich) repeats.Curated
Contains 4 LRRCT domains.Curated
Contains 4 LRRNT domains.Curated

Keywords - Domaini

EGF-like domain, Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4237. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000116120.
HOVERGENiHBG057959.
InParanoidiO88280.
KOiK06850.
PhylomeDBiO88280.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
3.80.10.10. 6 hits.
InterProiIPR013320. ConA-like_dom.
IPR000483. Cys-rich_flank_reg_C.
IPR006207. Cys_knot_C.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR032675. L_dom-like.
IPR001791. Laminin_G.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PfamiPF00008. EGF. 6 hits.
PF12661. hEGF. 1 hit.
PF02210. Laminin_G_2. 1 hit.
PF13855. LRR_8. 5 hits.
PF01463. LRRCT. 4 hits.
PF01462. LRRNT. 3 hits.
[Graphical view]
SMARTiSM00041. CT. 1 hit.
SM00181. EGF. 9 hits.
SM00179. EGF_CA. 9 hits.
SM00282. LamG. 1 hit.
SM00369. LRR_TYP. 18 hits.
SM00082. LRRCT. 4 hits.
SM00013. LRRNT. 4 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF52058. SSF52058. 4 hits.
SSF57184. SSF57184. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00022. EGF_1. 9 hits.
PS01186. EGF_2. 7 hits.
PS50026. EGF_3. 9 hits.
PS01187. EGF_CA. 2 hits.
PS50025. LAM_G_DOMAIN. 1 hit.
PS51450. LRR. 20 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGRTGAGA AVRARLALAL ALASILSGPP AAACPTKCTC SAASVDCHGL
60 70 80 90 100
GLRAVPRGIP RNAERLDLDR NNITRITKMD FTGLKNLRVL HLEDNQVSVI
110 120 130 140 150
ERGAFQDLKQ LERLRLNKNK LQVLPELLFQ STPKLTRLDL SENQIQGIPR
160 170 180 190 200
KAFRGVTGVK NLQLDNNHIS CIEDGAFRAL RDLEILTLNN NNISRILVTS
210 220 230 240 250
FNHMPKIRTL RLHSNHLYCD CHLAWLSDWL RQRRTIGQFT LCMAPVHLRG
260 270 280 290 300
FSVADVQKKE YVCPGPHSEA PACNANSLSC PSACSCSNNI VDCRGKGLTE
310 320 330 340 350
IPANLPEGIV EIRLEQNSIK SIPAGAFIQY KKLKRIDISK NQISDIAPDA
360 370 380 390 400
FQGLKSLTSL VLYGNKITEI PKGLFDGLVS LQLLLLNANK INCLRVNTFQ
410 420 430 440 450
DLQNLNLLSL YDNKLQTISK GLFAPLQSIQ TLHLAQNPFV CDCHLKWLAD
460 470 480 490 500
YLQDNPIETS GARCSSPRRL ANKRISQIKS KKFRCSGSED YRNRFSSECF
510 520 530 540 550
MDLVCPEKCR CEGTIVDCSN QKLSRIPSHL PEYTTDLRLN DNDIAVLEAT
560 570 580 590 600
GIFKKLPNLR KINLSNNRIK EVREGAFDGA AGVQELMLTG NQLETMHGRM
610 620 630 640 650
FRGLSGLKTL MLRSNLISCV NNDTFAGLSS VRLLSLYDNR ITTISPGAFT
660 670 680 690 700
TLVSLSTINL LSNPFNCNCH MAWLGRWLRK RRIVSGNPRC QKPFFLKEIP
710 720 730 740 750
IQDVAIQDFT CEGNEENSCQ LSPRCPEQCT CVETVVRCSN RGLHTLPKGM
760 770 780 790 800
PKDVTELYLE GNHLTAVPKE LSTFRQLTLI DLSNNSISML TNHTFSNMSH
810 820 830 840 850
LSTLILSYNR LRCIPVHAFN GLRSLRVLTL HGNDISSVPE GSFNDLTSLS
860 870 880 890 900
HLALGINPLH CDCSLRWLSE WIKAGYKEPG IARCSSPESM ADRLLLTTPT
910 920 930 940 950
HRFQCKGPVD INIVAKCNAC LSSPCKNNGT CSQDPVEQYR CTCPYSYKGK
960 970 980 990 1000
DCTVPINTCV QNPCQHGGTC HLSESHRDGF SCSCPLGFEG QRCEINPDDC
1010 1020 1030 1040 1050
EDNDCENSAT CVDGINNYAC VCPPNYTGEL CDEVIDYCVP EMNLCQHEAK
1060 1070 1080 1090 1100
CISLDKGFRC ECVPGYSGKL CETDNDDCVA HKCRHGAQCV DAVNGYTCIC
1110 1120 1130 1140 1150
PQGFSGLFCE HPPPMVLLQT SPCDQYECQN GAQCIVVQQE PTCRCPPGFA
1160 1170 1180 1190 1200
GPRCEKLITV NFVGKDSYVE LASAKVRPQA NISLQVATDK DNGILLYKGD
1210 1220 1230 1240 1250
NDPLALELYQ GHVRLVYDSL SSPPTTVYSV ETVNDGQFHS VELVMLNQTL
1260 1270 1280 1290 1300
NLVVDKGAPK SLGKLQKQPA VGINSPLYLG GIPTSTGLSA LRQGADRPLG
1310 1320 1330 1340 1350
GFHGCIHEVR INNELQDFKA LPPQSLGVSP GCKSCTVCRH GLCRSVEKDS
1360 1370 1380 1390 1400
VVCECHPGWT GPLCDQEAQD PCLGHSCSHG TCVATGNSYV CKCAEGYEGP
1410 1420 1430 1440 1450
LCDQKNDSAN ACSAFKCHHG QCHISDRGEP YCLCQPGFSG NHCEQENPCL
1460 1470 1480 1490 1500
GEIVREAIRR QKDYASCATA SKVPIMVCRG GCGSQCCQPI RSKRRKYVFQ
1510 1520
CTDGSSFVEE VERHLECGCR ECS
Length:1,523
Mass (Da):167,768
Last modified:November 1, 1998 - v1
Checksum:i6CE1B7AF9244478E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011531 mRNA. Translation: BAA32461.1.
PIRiT13953.
RefSeqiNP_112611.1. NM_031321.1.
UniGeneiRn.12298.

Genome annotation databases

GeneIDi83467.
KEGGirno:83467.
UCSCiRGD:69311. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011531 mRNA. Translation: BAA32461.1.
PIRiT13953.
RefSeqiNP_112611.1. NM_031321.1.
UniGeneiRn.12298.

3D structure databases

ProteinModelPortaliO88280.
SMRiO88280. Positions 505-712.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88280. 1 interaction.
STRINGi10116.ENSRNOP00000009714.

Proteomic databases

PaxDbiO88280.
PRIDEiO88280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83467.
KEGGirno:83467.
UCSCiRGD:69311. rat.

Organism-specific databases

CTDi6586.
RGDi69311. Slit3.

Phylogenomic databases

eggNOGiKOG4237. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000116120.
HOVERGENiHBG057959.
InParanoidiO88280.
KOiK06850.
PhylomeDBiO88280.

Miscellaneous databases

PROiO88280.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
3.80.10.10. 6 hits.
InterProiIPR013320. ConA-like_dom.
IPR000483. Cys-rich_flank_reg_C.
IPR006207. Cys_knot_C.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR032675. L_dom-like.
IPR001791. Laminin_G.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PfamiPF00008. EGF. 6 hits.
PF12661. hEGF. 1 hit.
PF02210. Laminin_G_2. 1 hit.
PF13855. LRR_8. 5 hits.
PF01463. LRRCT. 4 hits.
PF01462. LRRNT. 3 hits.
[Graphical view]
SMARTiSM00041. CT. 1 hit.
SM00181. EGF. 9 hits.
SM00179. EGF_CA. 9 hits.
SM00282. LamG. 1 hit.
SM00369. LRR_TYP. 18 hits.
SM00082. LRRCT. 4 hits.
SM00013. LRRNT. 4 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF52058. SSF52058. 4 hits.
SSF57184. SSF57184. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00022. EGF_1. 9 hits.
PS01186. EGF_2. 7 hits.
PS50026. EGF_3. 9 hits.
PS01187. EGF_CA. 2 hits.
PS50025. LAM_G_DOMAIN. 1 hit.
PS51450. LRR. 20 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening."
    Nakayama M., Nakajima D., Nagase T., Nomura N., Seki N., Ohara O.
    Genomics 51:27-34(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Spatiotemporal expression patterns of slit and robo genes in the rat brain."
    Marillat V., Cases O., Nguyen-Ba-Charvet K.T., Tessier-Lavigne M., Sotelo C., Chedotal A.
    J. Comp. Neurol. 442:130-155(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiSLIT3_RAT
AccessioniPrimary (citable) accession number: O88280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.