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O88278 (CELR3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cadherin EGF LAG seven-pass G-type receptor 3
Alternative name(s):
Multiple epidermal growth factor-like domains protein 2
Short name=Multiple EGF-like domains protein 2
Gene names
Name:Celsr3
Synonyms:Megf2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length3313 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor that may have an important role in cell/cell signaling during nervous system formation.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in the brain. Expressed in cerebellum, olfactory bulb, cerebral cortex, hippocampus and brain stem.

Post-translational modification

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 9 cadherin domains.

Contains 8 EGF-like domains.

Contains 1 GPS domain.

Contains 1 laminin EGF-like domain.

Contains 2 laminin G-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 33133282Cadherin EGF LAG seven-pass G-type receptor 3
PRO_0000012920

Regions

Topological domain32 – 25382507Extracellular Potential
Transmembrane2539 – 255921Helical; Name=1; Potential
Topological domain2560 – 257011Cytoplasmic Potential
Transmembrane2571 – 259121Helical; Name=2; Potential
Topological domain2592 – 25998Extracellular Potential
Transmembrane2600 – 262021Helical; Name=3; Potential
Topological domain2621 – 264121Cytoplasmic Potential
Transmembrane2642 – 266221Helical; Name=4; Potential
Topological domain2663 – 267917Extracellular Potential
Transmembrane2680 – 270021Helical; Name=5; Potential
Topological domain2701 – 272424Cytoplasmic Potential
Transmembrane2725 – 274521Helical; Name=6; Potential
Topological domain2746 – 27527Extracellular Potential
Transmembrane2753 – 277321Helical; Name=7; Potential
Topological domain2774 – 3313540Cytoplasmic Potential
Domain317 – 424108Cadherin 1
Domain425 – 536112Cadherin 2
Domain537 – 642106Cadherin 3
Domain643 – 747105Cadherin 4
Domain748 – 849102Cadherin 5
Domain850 – 952103Cadherin 6
Domain953 – 1058106Cadherin 7
Domain1059 – 1160102Cadherin 8
Domain1161 – 125797Cadherin 9
Domain1366 – 142459EGF-like 1; calcium-binding
Domain1426 – 146237EGF-like 2; calcium-binding
Domain1466 – 150540EGF-like 3; calcium-binding
Domain1506 – 1710205Laminin G-like 1
Domain1713 – 174937EGF-like 4; calcium-binding
Domain1753 – 1935183Laminin G-like 2
Domain1937 – 197236EGF-like 5; calcium-binding
Domain1973 – 201139EGF-like 6; calcium-binding
Domain2012 – 204433EGF-like 7; calcium-binding
Domain2046 – 208136EGF-like 8; calcium-binding
Domain2068 – 211548Laminin EGF-like
Domain2475 – 252753GPS

Amino acid modifications

Modified residue19541(3R)-3-hydroxyaspartate Potential
Modified residue21171Phosphotyrosine Ref.2
Modified residue30501Phosphotyrosine Ref.2
Modified residue30981Phosphoserine Ref.2
Glycosylation6231N-linked (GlcNAc...) Potential
Glycosylation8381N-linked (GlcNAc...) Potential
Glycosylation11731N-linked (GlcNAc...) Potential
Glycosylation12131N-linked (GlcNAc...) Potential
Glycosylation13081N-linked (GlcNAc...) Potential
Glycosylation13181N-linked (GlcNAc...) Potential
Glycosylation16401N-linked (GlcNAc...) Potential
Glycosylation17041N-linked (GlcNAc...) Potential
Glycosylation17611N-linked (GlcNAc...) Potential
Glycosylation20441N-linked (GlcNAc...) Potential
Glycosylation21731N-linked (GlcNAc...) Potential
Glycosylation21921N-linked (GlcNAc...) Potential
Glycosylation23821N-linked (GlcNAc...) Potential
Glycosylation24721N-linked (GlcNAc...) Potential
Glycosylation25041N-linked (GlcNAc...) Potential
Disulfide bond1370 ↔ 1381 By similarity
Disulfide bond1375 ↔ 1412 By similarity
Disulfide bond1414 ↔ 1423 By similarity
Disulfide bond1430 ↔ 1441 By similarity
Disulfide bond1435 ↔ 1450 By similarity
Disulfide bond1452 ↔ 1461 By similarity
Disulfide bond1470 ↔ 1481 By similarity
Disulfide bond1475 ↔ 1491 By similarity
Disulfide bond1493 ↔ 1504 By similarity
Disulfide bond1684 ↔ 1710 By similarity
Disulfide bond1717 ↔ 1728 By similarity
Disulfide bond1722 ↔ 1737 By similarity
Disulfide bond1739 ↔ 1748 By similarity
Disulfide bond1906 ↔ 1935 By similarity
Disulfide bond1941 ↔ 1952 By similarity
Disulfide bond1946 ↔ 1961 By similarity
Disulfide bond1963 ↔ 1972 By similarity
Disulfide bond1976 ↔ 1987 By similarity
Disulfide bond1981 ↔ 1999 By similarity
Disulfide bond2001 ↔ 2010 By similarity
Disulfide bond2018 ↔ 2031 By similarity
Disulfide bond2033 ↔ 2043 By similarity
Disulfide bond2050 ↔ 2065 By similarity
Disulfide bond2052 ↔ 2068 By similarity
Disulfide bond2070 ↔ 2080 By similarity
Disulfide bond2089 ↔ 2098 By similarity
Disulfide bond2101 ↔ 2113 By similarity

Sequences

Sequence LengthMass (Da)Tools
O88278 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: B11DA09517288764

FASTA3,313359,355
        10         20         30         40         50         60 
MARRPLWWGL PGPSTPLLLL LLFSLFPSSR EEMGGGGDQG WDPGVATATG PRAQIGSGAV 

        70         80         90        100        110        120 
ALCPESPGVW EDGDPGLGVR EPVFMKLRVG RQNARNGRGA PEQPNREPVV QALGSREQEA 

       130        140        150        160        170        180 
GQGSGYLLCW HPEISSCGRT GHLRRGSLPL DALSPGDSDL RNSSPHPSEL LAQPDSPRPV 

       190        200        210        220        230        240 
AFQRNGRRSI RKRVETFRCC GKLWEPGHKG QGERSATSTV DRGPLRRDCL PGSLGSGLGE 

       250        260        270        280        290        300 
DSAPRAVRTA PAPGSAPHES RTAPERMRSR GLFRRGFLFE RPGPRPPGFP TGAEAKRILS 

       310        320        330        340        350        360 
TNQARSRRAA NRHPQFPQYN YQTLVPENEA AGTAVLRVVA QDPDPGEAGR LVYSLAALMN 

       370        380        390        400        410        420 
SRSLELFSID PQSGLIRTAA ALDRESMERH YLRVTAQDHG SPRLSATTMV AVTVADRNDH 

       430        440        450        460        470        480 
APVFEQAQYR ETLRENVEEG YPILQLRATD GDAPPNANLR YRFVGSPAAR TAAAAAFEID 

       490        500        510        520        530        540 
PRSGLISTSG RVDREHMESY ELVVEASDQG QEPGPRSATV RVHITVLDEN DNAPQFSEKR 

       550        560        570        580        590        600 
YVAQVREDVR PHTVVLRVTA TDKDKDANGL VHYNIISGNS RGHFAIDSLT GEIQVMAPLD 

       610        620        630        640        650        660 
FEAEREYALR IRAQDAGRPP LSNNTGLASI QVVDINDHSP IFVSTPFQVS VLENAPLGHS 

       670        680        690        700        710        720 
VIHIQAVDAD HGENSRLEYS LTGVASDTPF VINSATGWVS VSGPLDRESV EHYFFGVEAR 

       730        740        750        760        770        780 
DHGSPPLSAS ASVTVTVLDV NDNRPEFTMK EYHLRLNEDA AVGTSVVSVT AVDRDANSAI 

       790        800        810        820        830        840 
SYQITGGNTR NRFAISTQGG MGLVTLALPL DYKQERYFKL VLTASDRALH DHCYVHINIT 

       850        860        870        880        890        900 
DANTHRPVFQ SAHYSVSMNE DRPVGSTVVV ISASDDDVGE NARITYLLED NLPQFRIDAD 

       910        920        930        940        950        960 
SGAITLQAPL DYEDQVTYTL AITARDNGIP QKADTTYVEV MVNDVNDNAP QFVASHYTGL 

       970        980        990       1000       1010       1020 
VSEDAPPFTS VLQISATDRD AHANGRVQYT FQNGEDGDGD FTIEPTSGIV RTVRRLDREA 

      1030       1040       1050       1060       1070       1080 
VPVYELTAYA VDRGVPPLRT PVSIQVTVQD VNDNAPVFPA EEFEVRVKEN SIVGSVVAQI 

      1090       1100       1110       1120       1130       1140 
TAVDPDDGPN AHIMYQIVEG NIPELFQMDI FSGELTALID LDYEARQEYV IVVQATSAPL 

      1150       1160       1170       1180       1190       1200 
VSRATVHVRL VDQNDNSPVL NNFQILFNNY VSNRSDTFPS GIIGRIPAYD PDVSDHLFYS 

      1210       1220       1230       1240       1250       1260 
FERGNELQLL VVNQTSGELR LSRKLDNNRP LVASMLVTVT DGLHSVTAQC VLRVVIITEE 

      1270       1280       1290       1300       1310       1320 
LLANSLTVRL ENMWQERFLS PLLGHFLEGV AAVLATPTED VFIFNIQNDT DVGGTVLNVS 

      1330       1340       1350       1360       1370       1380 
FSALAPRGAG AGAAGPWFSS EELQEQLYVR RAALAARSLL DVLPFDDNVC LREPCENYMK 

      1390       1400       1410       1420       1430       1440 
CVSVLRFDSS APFLASASTL FRPIQPIAGL RCRCPPGFTG DFCETELDLC YSNPCRNGGA 

      1450       1460       1470       1480       1490       1500 
CARREGGYTC VCRPRFTGED CELDTEAGRC VPGVCRNGGT CTNAPNGGFR CQCPAGGAFE 

      1510       1520       1530       1540       1550       1560 
GPRCEVAARS FPPSSFVMFR GLRQRFHLTL SLSFATVQPS GLLFYNGRLN EKHDFLALEL 

      1570       1580       1590       1600       1610       1620 
VAGQVRLTYS TGESSTVVSP TVPGGLSDGQ WHTVHLRYYN KPRTDALGGA QGPSKDKVAV 

      1630       1640       1650       1660       1670       1680 
LSVDDCNVAV ALRFGAEIGN YSCAAAGVQT SSKKSLDLTG PLLLGGVPNL PENFPVSRKD 

      1690       1700       1710       1720       1730       1740 
FIGCMRDLHI DGRRVDMAAF VANNGTTAGC QAKSHFCASG PCKNGGLCSE RWGGFSCDCP 

      1750       1760       1770       1780       1790       1800 
VGFGGKDCRL TMAHPYHFQG NGTLSWDFGN DMPVSVPWYL GLSFRTRATK GVLMQVQLGP 

      1810       1820       1830       1840       1850       1860 
HSVLLCKLDQ GLLSVTLSRA SGHAVHLLLD QMTVSDGRWH DLRLELQEEP GGRRGHHIFM 

      1870       1880       1890       1900       1910       1920 
VSLDFTLFQD TMAMGSELEG LKVKHLHVGG PPPSSKEEGP QGLVGCIQGV WTGFTPFGSS 

      1930       1940       1950       1960       1970       1980 
ALPPPSHRIN VEPGCTVTNP CASGPCPPHA NCKDLWQTFS CTCWPGYYGP GCVDACLLNP 

      1990       2000       2010       2020       2030       2040 
CQNQGSCRHL QGGPHGYTCD CASGYFGQHC EHRMDQQCPR GWWGSPTCGP CNCDVHKGFD 

      2050       2060       2070       2080       2090       2100 
PNCNKTSGQC HCKEFHYRPR GSDSCLPCDC YPVGSTSRSC APHSGQCPCR PGALGRQCNS 

      2110       2120       2130       2140       2150       2160 
CDSPFAEVTA SGCRVLYDAC PKSLRSGVWW PQTKFGVLAT VPCPRGALGL RGTGAAVRLC 

      2170       2180       2190       2200       2210       2220 
DEDHGWLEPD FFNCTSPAFR ELSLLLDGLE LNKTALDTVE AKKLAQRLRE VTGQTDHYFS 

      2230       2240       2250       2260       2270       2280 
QDVRVTARLL AYLLAFESHQ QGFGLTATQD AHFNENLLWA GSALLAPETG DLWAALGQRA 

      2290       2300       2310       2320       2330       2340 
PGGSPGSAGL VRHLEEYAAT LARNMDLTYL NPVGLVTPNI MLSIDRMEQP SSSQGAHRYP 

      2350       2360       2370       2380       2390       2400 
RYHSNLFRGQ DAWDPHTHVL LPSQSPQPSP SEVLPTSSNA ENATASGVVS PPAPLEPESE 

      2410       2420       2430       2440       2450       2460 
PGISIVILLV YRALGGLLPA QFQAERRGAR LPQNPVMNSP VVSVAVFRGR NFLRGALVSP 

      2470       2480       2490       2500       2510       2520 
INLEFRLLQT ANRSKAICVQ WDPPGPADQH GMWTARDCEL VHRNGSHARC RCSRTGTFGV 

      2530       2540       2550       2560       2570       2580 
LMDASPRERL EGDLELLAVF THVVVAASVT ALVLTAAVLL SLRSLKSNVR GIHANVAAAL 

      2590       2600       2610       2620       2630       2640 
GVAELLFLLG IHRTHNQLLC TVVAILLHYF FLSTFAWLLV QGLHLYRMQV EPRNVDRGAM 

      2650       2660       2670       2680       2690       2700 
RFYHALGWGV PAVLLGLAVG LDPEGYGNPD FCWISIHEPL IWSFAGPIVL VIVMNGIMFL 

      2710       2720       2730       2740       2750       2760 
LAARTSCSTG QREAKKTSVL RTLRSSFLLL LLVSASWLFG LLAVNHSVLA FHYLHAGLCG 

      2770       2780       2790       2800       2810       2820 
LQGLAVLLLF CVLNADARAA WTPACLGKKA APEETRPAPG PGSGAYNNTA LFEESGLIRI 

      2830       2840       2850       2860       2870       2880 
TLGASTVSSV SSARSGRAQD QDSQRGRSYL RDNVLVRHGS TAEHAEHSLQ AHAGPTDLDV 

      2890       2900       2910       2920       2930       2940 
AMFHRDAGAD SDSDSDLSLE EERSLSIPSS ESEDNGRTRG RFQRPLRRAA QSERLLAHPK 

      2950       2960       2970       2980       2990       3000 
DVDGNDLLSY WPALGECEAA PCALQAWGSE RRLGLDSNKD AANNNQPELA LTSGDETSLG 

      3010       3020       3030       3040       3050       3060 
RAQRQRKGIL KNRLQYPLVP QTRGTPELSW CRAATLGHRA VPAASYGRIY AGGGTGSLSQ 

      3070       3080       3090       3100       3110       3120 
PASRYSSREQ LDLLLRRQLS RERLEEVPVP APVLHPLSRP GSQERLDTAP ARLEPRDRGS 

      3130       3140       3150       3160       3170       3180 
TLPRRQPPRD YPGTMAGRFG SRDALDLGAP REWLSTLPPP RRNRDLDPQH PPLPLSPQRP 

      3190       3200       3210       3220       3230       3240 
LSRDPLLPSR PLDSLSRISN SRERLDQVPS RHPSREALGP APQLLRARED PASGPSHGPS 

      3250       3260       3270       3280       3290       3300 
TEQLDILSSI LASFNSSALS SVQSSSTPSG PHTTATPSAT ASALGPSTPR SATSHSISEL 

      3310 
SPDSEVPRSE GHS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening."
Nakayama M., Nakajima D., Nagase T., Nomura N., Seki N., Ohara O.
Genomics 51:27-34(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-2117; TYR-3050 AND SER-3098, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011528 mRNA. Translation: BAA32459.1.
RefSeqNP_112610.1. NM_031320.1.
UniGeneRn.14558.

3D structure databases

ProteinModelPortalO88278.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000041011.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteO88278.

Proteomic databases

PaxDbO88278.
PRIDEO88278.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000040661; ENSRNOP00000041011; ENSRNOG00000034005.
GeneID83466.
KEGGrno:83466.
UCSCRGD:621787. rat.

Organism-specific databases

CTD1951.
RGD621787. Celsr3.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00750000117340.
HOGENOMHOG000231346.
HOVERGENHBG050887.
InParanoidO88278.
KOK04602.
OMAYRFVGPP.
OrthoDBEOG7BP81K.
PhylomeDBO88278.

Gene expression databases

GenevestigatorO88278.

Family and domain databases

Gene3D2.60.120.200. 2 hits.
2.60.40.60. 9 hits.
InterProIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR022624. DUF3497.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002049. EGF_laminin.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR001791. Laminin_G.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF00028. Cadherin. 8 hits.
PF12003. DUF3497. 1 hit.
PF00008. EGF. 3 hits.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF00053. Laminin_EGF. 1 hit.
PF02210. Laminin_G_2. 2 hits.
[Graphical view]
PRINTSPR00205. CADHERIN.
PR00249. GPCRSECRETIN.
SMARTSM00112. CA. 9 hits.
SM00181. EGF. 6 hits.
SM00180. EGF_Lam. 1 hit.
SM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00282. LamG. 2 hits.
[Graphical view]
SUPFAMSSF49313. SSF49313. 9 hits.
SSF49899. SSF49899. 2 hits.
PROSITEPS00010. ASX_HYDROXYL. 1 hit.
PS00232. CADHERIN_1. 7 hits.
PS50268. CADHERIN_2. 8 hits.
PS00022. EGF_1. 6 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 6 hits.
PS01248. EGF_LAM_1. 1 hit.
PS50027. EGF_LAM_2. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50025. LAM_G_DOMAIN. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio615859.

Entry information

Entry nameCELR3_RAT
AccessionPrimary (citable) accession number: O88278
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries