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O88277 (FAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protocadherin Fat 2
Alternative name(s):
Multiple epidermal growth factor-like domains protein 1
Short name=Multiple EGF-like domains protein 1
Gene names
Name:Fat2
Synonyms:Fath2, Megf1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length4351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May modulate the extracellular space surronding parallel fibers of cerebellar during development. Plays a role in the migration of epidermal cells By similarity.

Subunit structure

Homodimer. Ref.3

Subcellular location

Cell membrane; Single-pass membrane protein Potential. Cell junction By similarity. Nucleus By similarity.

Tissue specificity

Cerebellum-specific expression. Expressed in thin parallel fibers of cerebellar granule cells. Ref.1 Ref.3

Developmental stage

In the developing cerebellum, expressed in granule cells in the inner external germinal layer and in migrating granule cells whereas proliferating cells in the outer extessornal germinal layer did not shown expression. Expression levels in the internal granule cell layer peak during the third postnatal week and remain considerably high in the adult cerebellum. Ref.3

Sequence similarities

Contains 33 cadherin domains.

Contains 2 EGF-like domains.

Contains 1 laminin G-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 43514333Protocadherin Fat 2
PRO_0000004019

Regions

Topological domain19 – 40504032Extracellular Potential
Transmembrane4051 – 407121Helical; Potential
Topological domain4072 – 4351280Cytoplasmic Potential
Domain34 – 148115Cadherin 1
Domain149 – 256108Cadherin 2
Domain363 – 45896Cadherin 3
Domain459 – 564106Cadherin 4
Domain565 – 669105Cadherin 5
Domain716 – 820105Cadherin 6
Domain821 – 925105Cadherin 7
Domain926 – 1032107Cadherin 8
Domain1033 – 1142110Cadherin 9
Domain1138 – 1242105Cadherin 10
Domain1243 – 1346104Cadherin 11
Domain1350 – 144899Cadherin 12
Domain1449 – 1555107Cadherin 13
Domain1556 – 1660105Cadherin 14
Domain1661 – 175898Cadherin 15
Domain1759 – 1872114Cadherin 16
Domain1873 – 196896Cadherin 17
Domain1969 – 2070102Cadherin 18
Domain2071 – 2171101Cadherin 19
Domain2172 – 2272101Cadherin 20
Domain2273 – 2379107Cadherin 21
Domain2380 – 2481102Cadherin 22
Domain2482 – 2585104Cadherin 23
Domain2586 – 2692107Cadherin 24
Domain2693 – 2799107Cadherin 25
Domain2800 – 2908109Cadherin 26
Domain2909 – 3013105Cadherin 27
Domain3014 – 3115102Cadherin 28
Domain3116 – 3220105Cadherin 29
Domain3221 – 3323103Cadherin 30
Domain3324 – 3428105Cadherin 31
Domain3429 – 3533105Cadherin 32
Domain3534 – 363198Cadherin 33
Domain3775 – 3946172Laminin G-like
Domain3949 – 398638EGF-like 1
Domain3988 – 402437EGF-like 2

Amino acid modifications

Glycosylation391N-linked (GlcNAc...) Potential
Glycosylation2101N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation3301N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Potential
Glycosylation5681N-linked (GlcNAc...) Potential
Glycosylation6271N-linked (GlcNAc...) Potential
Glycosylation7891N-linked (GlcNAc...) Potential
Glycosylation9961N-linked (GlcNAc...) Potential
Glycosylation11751N-linked (GlcNAc...) Potential
Glycosylation12761N-linked (GlcNAc...) Potential
Glycosylation14171N-linked (GlcNAc...) Potential
Glycosylation18991N-linked (GlcNAc...) Potential
Glycosylation19981N-linked (GlcNAc...) Potential
Glycosylation20071N-linked (GlcNAc...) Potential
Glycosylation21021N-linked (GlcNAc...) Potential
Glycosylation21651N-linked (GlcNAc...) Potential
Glycosylation21831N-linked (GlcNAc...) Potential
Glycosylation23251N-linked (GlcNAc...) Potential
Glycosylation23681N-linked (GlcNAc...) Potential
Glycosylation23871N-linked (GlcNAc...) Potential
Glycosylation24301N-linked (GlcNAc...) Potential
Glycosylation24701N-linked (GlcNAc...) Potential
Glycosylation25471N-linked (GlcNAc...) Potential
Glycosylation25971N-linked (GlcNAc...) Potential
Glycosylation31271N-linked (GlcNAc...) Potential
Glycosylation32781N-linked (GlcNAc...) Potential
Glycosylation33121N-linked (GlcNAc...) Potential
Glycosylation34321N-linked (GlcNAc...) Potential
Glycosylation36031N-linked (GlcNAc...) Potential
Glycosylation37701N-linked (GlcNAc...) Potential
Glycosylation37741N-linked (GlcNAc...) Potential
Glycosylation38151N-linked (GlcNAc...) Potential
Glycosylation38421N-linked (GlcNAc...) Potential
Glycosylation38751N-linked (GlcNAc...) Potential
Glycosylation39061N-linked (GlcNAc...) Potential
Glycosylation39911N-linked (GlcNAc...) Potential
Disulfide bond3914 ↔ 3946 By similarity
Disulfide bond3953 ↔ 3964 By similarity
Disulfide bond3958 ↔ 3974 By similarity
Disulfide bond3976 ↔ 3985 By similarity
Disulfide bond3992 ↔ 4003 By similarity
Disulfide bond3997 ↔ 4012 By similarity
Disulfide bond4014 ↔ 4023 By similarity

Sequences

Sequence LengthMass (Da)Tools
O88277 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: C564E7F98EBB3888

FASTA4,351480,654
        10         20         30         40         50         60 
MTLVLLGLAI LLLHRAACEK SLEETIPPLS WRFTHSLYNA TIYENSAPKT YVESPVKMGM 

        70         80         90        100        110        120 
YLAEPHWVVK YRIISGDAAG VFKTEEHVVG NFCFLRIRTK SSNTALLNRE VRDSYTLIVQ 

       130        140        150        160        170        180 
ASDKSLEFEA LTQVVVHILD QNDLKPLFSP PSYRVTISED RPLKSPICKV TATDADLGQN 

       190        200        210        220        230        240 
AEFYYAFNAR SEVFAIHPTS GVVTVAGKLN VTRRGKYELQ VLAVDRMRKI SEGNGFGNLA 

       250        260        270        280        290        300 
SLVIRVEPVH RKPPAINLVV LNPPEGDEGD IYAIVTVDTN GSGAEVDSLE VVGGDPGKYF 

       310        320        330        340        350        360 
KVLRSYAQGN EFNLVAVRDI NWAEHPHGFN ISLQTHSWSR FPPHSIIRAF HLPSWKLANL 

       370        380        390        400        410        420 
RFEKAVYRVK LSEFSPPGSR VALVKVTTAL PNLRYSLKPS SRNTAFKLNA RTGLITTTKL 

       430        440        450        460        470        480 
VDFHEQNQYQ LHVKTSLGQA TTTVIIDIVD CNNHAPVFNR SSYEGTLDEN IPPGTSVLTV 

       490        500        510        520        530        540 
TATDQDHGDN GHITYSIAGP KAVPFSIDPL LGVISTTKPM DYELMKRIYT FRVRASDWGS 

       550        560        570        580        590        600 
PFRQEKEVSV SLRLKNLNDN QPMFEEVNCT VSLRQDVPVG KSIMAVSAID MDELQNLKYE 

       610        620        630        640        650        660 
IVSGNEQDYF HLNHFSGVIS LKRSFMNLTA VRPTIYSLKI TASDGKNYAS PTTLKVTVVK 

       670        680        690        700        710        720 
DPHSEVPVQC DKTGVLTHIT KTILQSAGLQ SQELGEEEFT SLSNYQINHH SPQFEDHFPQ 

       730        740        750        760        770        780 
SIDILEQVPI NTPLARLAAT DPDTGFHGKL VYVISDGNEE GCFDIELETG LLMVAAALDY 

       790        800        810        820        830        840 
ETTSFYVLNV TVYDLGTPPK SSWKLLTVTV KDWNDNPPRF PPGGYQLTIS EDTEVGTTIA 

       850        860        870        880        890        900 
ELKTEDADSE DNRRVRYTLL TPTEKFSLHP FTGELVVTGH LDRESESQYI LKAEARDQPT 

       910        920        930        940        950        960 
KGHQLFSVTD LIVTLEDIND NPPQCITEHR RLKVPEDMPL GTVLTFLDAS DPDLGPAGEV 

       970        980        990       1000       1010       1020 
KYILVEDAHG TFQVHPMTGA LSLEKELDFE RRAGYNLSFW ASDSGKPLSR RTLCHVEVLV 

      1030       1040       1050       1060       1070       1080 
MDVNENLHSP HFSSFVYQGQ VQENSPAGTP VMVVTAQDDD SGLDGELQYF LRAGTGLETF 

      1090       1100       1110       1120       1130       1140 
SINQDTGMLE TLAPLDREFT PYYWLTVLAV DRGSVPLSAV TEVYIEVTDI NDNIPSMSRP 

      1150       1160       1170       1180       1190       1200 
VFYPSVLEDA PLGTSVLQLE AWDPDSSSQG KLTFNLTSGN HLGHFIVHPF TGLLTTAKQL 

      1210       1220       1230       1240       1250       1260 
DRENKDEYVL EVTVQDNGDP SLRSTSRVVV CILDVNDNPP MFSHKLFNVR LSERLSPLSP 

      1270       1280       1290       1300       1310       1320 
EPVYRLVASD PDEGLNGSVT YSIEESDEES FRIDPVTGVV SSSSTFAAGE YNILTIKATD 

      1330       1340       1350       1360       1370       1380 
SGQPALSTSV RLHIEWIPQP RPSSIPLSFD ESYYSFTVME TDPVNHMVGV ISVEGRPGLF 

      1390       1400       1410       1420       1430       1440 
WFHISDGDKD MDFDIEKTTG SIVIARPLDT RRKSSYNLTV EVTDGFHTIA TQVHIFMIAN 

      1450       1460       1470       1480       1490       1500 
INHHRPQFLQ DHYEIRVPQD TLPGVELLRV QATDQDHGKG LIYTILSSQD PGSANLFQLD 

      1510       1520       1530       1540       1550       1560 
PSSGVLVTVG TLELHSGPSQ HILTVMVRDQ EMPIKRNFVW VTIHVEDGNL HSPHFTQLRY 

      1570       1580       1590       1600       1610       1620 
EANVPDTTAP GTELLQVRAV DADRGANAEI HYSFLKGNSD GFFNIDSLLG IITVAQRLYH 

      1630       1640       1650       1660       1670       1680 
VHLTRHALTV KAEDQGSPRR HDLALVVIHV HPSDSSAPVF SKDEYFIEIP ESVPIGSPIL 

      1690       1700       1710       1720       1730       1740 
LLSAGSSSEV TYELREGNKD SVFSMNSYSG LISTQKRLDH EKVPSYRLRI RGSNMAGVFT 

      1750       1760       1770       1780       1790       1800 
EVVALVYIID ENDNPPAFGK PTFLGHISEA APLHSLILGE DNSPLVVRAS DSDREANSLL 

      1810       1820       1830       1840       1850       1860 
VYKILEPEAL KFFKIDPSMG TLTTTSELDF EDTPLFQFNI YVHDQGTPIL FAPRSAKVII 

      1870       1880       1890       1900       1910       1920 
HVRDVNDSPP RFSEQIYEVA VVEPIHPGMG LLTVQAEDND SRVTYSIKTS NADEAVTIHP 

      1930       1940       1950       1960       1970       1980 
TTGQISVVNP ATLRLFQKFS IRASDGLYHD TAVVKISLTQ VLDKSLQFDQ DVYRARVTEN 

      1990       2000       2010       2020       2030       2040 
TPHRKALVIL GVHGNHLNDT LSYFLLNGTD LFHMIESAGV LQTRGGTFDR EQQDTHEVAV 

      2050       2060       2070       2080       2090       2100 
EVRDNRVPQR VAQALVRVSV EDVNDNIPEF QHLPYYTVIQ DGTEPGDVLF QVSATDKDLG 

      2110       2120       2130       2140       2150       2160 
ANGSVTYGFA EDYAYFRIDP YVGDISLKKP FDYQALNKYH LRVIARDSGI PPLQTEVEVH 

      2170       2180       2190       2200       2210       2220 
VTVRNKSNPL FQSPYYKVKV PENITLYTPI LHTQARSPEG LRLIYNIVEE EPLMLFTTDF 

      2230       2240       2250       2260       2270       2280 
KTGVLTVTGP LDYESKNKHV FTVRATDTAL GSFSEATVEV LVEDINDNPP TFSQLVYTTS 

      2290       2300       2310       2320       2330       2340 
VSEGSPAQTP VIQLLASDQD SGQNQDVSYQ IVEDGSDVSK FFRINGSTGE IFTIQELDYE 

      2350       2360       2370       2380       2390       2400 
THQHFRVKVR AMDKGDPPLT GETLVVVNVS DINDNPPKFR EPQYEANVSE LATCGHLVLK 

      2410       2420       2430       2440       2450       2460 
VQALDPDIGD TSRLEYLILS GNQDRHFSIN STSGIISMFN LCKKQLDSSY NLRVGASDGV 

      2470       2480       2490       2500       2510       2520 
FRATVPVYIN TTNANKYSPE FQQNVYEAEL AENAKVGTKV IELLAIDKDS GPYGTVDYTI 

      2530       2540       2550       2560       2570       2580 
INKLAGERFF INPRGQITTL QKLDRENSTE RVIAIKVMAR DGGGKVAFCT VKIILTDEND 

      2590       2600       2610       2620       2630       2640 
NAPQFKASGY TVSIPSNVSR DSPIIQVLAY DADEGRNADV TYSVDSTEDL AEEIIEVNPT 

      2650       2660       2670       2680       2690       2700 
TGVVKVKESL VGLENRAVDF NIKAQDGGPP HWDSLVPVRL QVVPNEIPLP KFSEPLYTFS 

      2710       2720       2730       2740       2750       2760 
APEDLPEGSE IGSVKAVAAQ DPIIYSLVQG TTPESNSDDV FSLDQDTGVL KVRKAMDHES 

      2770       2780       2790       2800       2810       2820 
TKWYQIDLMA HCPHEDTDLV SLVSVSIQVE DVNDNRPVFE ADPYKAFLTE NMPGGTTVIQ 

      2830       2840       2850       2860       2870       2880 
VTANDQDTGS DGQVSYRLSV EPGSNIHELF AVDSESGWIT TLQELDCETQ QTYRFYVVAF 

      2890       2900       2910       2920       2930       2940 
DHGQTIQLSS QALVEVSITD ENDNPPRFAS EDYRGSVVEN NEPGELVATL KTLDADVSDQ 

      2950       2960       2970       2980       2990       3000 
NRQVTCYITE GDPLGQFSIS QVGDEWRISS RKTLDREHIA KYLLRVTASD GKFQASVPVE 

      3010       3020       3030       3040       3050       3060 
VFVVDINDNS PQCSQLLYTG KVREDVTPGH FILKVSAIDV DMDTNAQITY SLHGPGAQEF 

      3070       3080       3090       3100       3110       3120 
KLDPHTGELT TLTVLDRERK DVYNLVAKAT DGGGQSCQAE VTLHIEDVND NAPRFFPSHC 

      3130       3140       3150       3160       3170       3180 
DVAVFDNTTV KTPVAVVFAR DPDQGANAQV VYSLTDSADG QFSIDATSGV IRLEKPLQVR 

      3190       3200       3210       3220       3230       3240 
ASSAVELTVR ASDLGTPIPL STLGTVTVSV VGLEDYLPIF LNAEHSTQVP EDAPIDMEVL 

      3250       3260       3270       3280       3290       3300 
HLATLTRPGS EKTGYHITGG NEQGKFRLDA HTGILYVNGS LDFETNPKYF LSIECSRKSS 

      3310       3320       3330       3340       3350       3360 
SSLSDVTTIV INVTDVNEHH PRFTHDLYTV RVLENAVVGD VILTVSASDD DGPVNSAITY 

      3370       3380       3390       3400       3410       3420 
SLVGGNQLGH FTINPKKGKL QVAKALDWEQ TPSYSLRLRA TDSGQPPLHE DTEVAVEVVD 

      3430       3440       3450       3460       3470       3480 
VNDNPPRFFQ LNYSTSVQEN SPIGIKVLQL ILDDPDSPQN GPPYFFRITE GNTGSVFRVT 

      3490       3500       3510       3520       3530       3540 
PDGWLVTAAS LSKKAREWYQ LHIEVSDSGL PPLSSSTLVR VQVTEQSRYP PSTLPLEISI 

      3550       3560       3570       3580       3590       3600 
TKGEEEFQGG MIGKIHATDR DPQDTLTYSL EQEGGLDRYF TVGASDGKII ASQGLPHGRY 

      3610       3620       3630       3640       3650       3660 
SFNVTVSDGT FTTTTGVHVH VWHMEPEVPQ QAVWLGFHQL TPEELVSDHW RNLQRFLSNL 

      3670       3680       3690       3700       3710       3720 
LDVKRANIHL ASLQPAEVTA GVDVLLVFER HSGTSYDLQE LASAIAHSVR EIEHSVGIRM 

      3730       3740       3750       3760       3770       3780 
RSALPVVPCQ GQSCQDQTCQ ETVSLEPRVG PSYSTARLSI LTPRHHLGRN CSCNGTTLRF 

      3790       3800       3810       3820       3830       3840 
SGQSYVQYRP LEAQNWQIHF YLKTLQPWAL LMFTNETASI SLKLANGFSH LEYHCPGGFY 

      3850       3860       3870       3880       3890       3900 
GNLSSRYPVN DGQWHSMLLE ERDTSVHLLV DITDNASLVI PEECQGLRTE RQLLLGGLVP 

      3910       3920       3930       3940       3950       3960 
SNPSSNVSLG FEGCLDAVVV NGERLELLGR EKKMEGRLET WALSQCCWPG TACSQSPCLN 

      3970       3980       3990       4000       4010       4020 
GGSCSPALGS GYLCRCPPPF SGRNCELGRE NCTSAPCQEG GTCVSSPEGT SCNCPHPYTG 

      4030       4040       4050       4060       4070       4080 
DRCEMEARGC SGGHCLITPE IKRGDWGQQE FLVITVALPL VIIATVGLLL YCRRRKSHKP 

      4090       4100       4110       4120       4130       4140 
VTMEDPDLLA RSIGVDTQAS PAIELDPLNT SSCNNLNQPE PSKTSVPNEL VTFGPSSKQR 

      4150       4160       4170       4180       4190       4200 
PMVCSVPPRL PPAAVSSHPG HEPIIKRTWS GEELVYPSGA AVWPPTYSRK KHWEYPHPET 

      4210       4220       4230       4240       4250       4260 
MQGTLPPSPR RHVGPAVMPD PTGLYGGFPF PLELENKRAP LPPRYSNQNL EDLMPPRPPS 

      4270       4280       4290       4300       4310       4320 
PREHLLAPCL NEYTAISYYH SQFRQGGGGP CLAEGGYKGV SMRLSRAGPS YADCEVNGGP 

      4330       4340       4350 
ATGRSQPRAP PNYEGSDMVE SDYGSCEEVM F 

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References

[1]"Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening."
Nakayama M., Nakajima D., Nagase T., Nomura N., Seki N., Ohara O.
Genomics 51:27-34(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 673-681; 2566-2572; 2968-2976 AND 3081-3088, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[3]"MEGF1/fat2 proteins containing extraordinarily large extracellular domains are localized to thin parallel fibers of cerebellar granule cells."
Nakayama M., Nakajima D., Yoshimura R., Endo Y., Ohara O.
Mol. Cell. Neurosci. 20:563-578(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBUNIT, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011527 mRNA. Translation: BAA32458.1.
PIRT00252.
RefSeqNP_075243.1. NM_022954.1.
UniGeneRn.137161.

3D structure databases

ProteinModelPortalO88277.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249241. 1 interaction.
STRING10116.ENSRNOP00000017106.

PTM databases

PhosphoSiteO88277.

Proteomic databases

PaxDbO88277.
PRIDEO88277.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID65048.
KEGGrno:65048.
UCSCRGD:620656. rat.

Organism-specific databases

CTD2196.
RGD620656. Fat2.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000046499.
HOVERGENHBG005641.
KOK16506.
PhylomeDBO88277.

Gene expression databases

GenevestigatorO88277.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
2.60.40.60. 33 hits.
InterProIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
[Graphical view]
PfamPF00028. Cadherin. 27 hits.
PF00008. EGF. 2 hits.
PF02210. Laminin_G_2. 1 hit.
[Graphical view]
PRINTSPR00205. CADHERIN.
SMARTSM00112. CA. 33 hits.
SM00181. EGF. 2 hits.
SM00282. LamG. 1 hit.
[Graphical view]
SUPFAMSSF49313. SSF49313. 33 hits.
SSF49899. SSF49899. 1 hit.
PROSITEPS00232. CADHERIN_1. 14 hits.
PS50268. CADHERIN_2. 33 hits.
PS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS50025. LAM_G_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613888.
PROO88277.

Entry information

Entry nameFAT2_RAT
AccessionPrimary (citable) accession number: O88277
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families