Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acyl-coenzyme A thioesterase 1

Gene

Acot1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232Charge relay systemBy similarity1
Active sitei324Charge relay systemBy similarity1
Active sitei358Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • acyl-CoA metabolic process Source: InterPro
  • lipid metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.2.2. 5301.
3.1.2.20. 5301.

Protein family/group databases

ESTHERiratno-acot1. Acyl-CoA_Thioesterase.
MEROPSiS09.A53.

Chemistry databases

SwissLipidsiSLP:000000588.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 1 (EC:3.1.2.2)
Short name:
Acyl-CoA thioesterase 1
Alternative name(s):
CTE-I
Inducible cytosolic acyl-coenzyme A thioester hydrolase
LACH2
Short name:
ACH2
Long chain acyl-CoA thioester hydrolase
Short name:
Long chain acyl-CoA hydrolase
Gene namesi
Name:Acot1
Synonyms:Cte1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70894. Acot1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002021572 – 419Acyl-coenzyme A thioesterase 1Add BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei416PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88267.
PRIDEiO88267.

PTM databases

iPTMnetiO88267.
PhosphoSitePlusiO88267.

Expressioni

Tissue specificityi

Expressed in liver.

Inductioni

In the liver, by peroxisome proliferator (Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013729.

Structurei

3D structure databases

ProteinModelPortaliO88267.
SMRiO88267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG410II3X. Eukaryota.
COG1073. LUCA.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiO88267.
KOiK01068.
PhylomeDBiO88267.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATLSLEPA GRSCWDEPLS ITVRGLVPEQ PVTLRAALRD EKGALFRARA
60 70 80 90 100
LYRADAHGEL DLARAPALGG SFTGLEPMGL IWAMEPERPF WRLVKRDVQT
110 120 130 140 150
PFVVELEVLD GHEPDGGRLL ARAVHERHFM APGVRRVPVR EGRVRATLFL
160 170 180 190 200
PPEPGPFPGI IDLFGVGGGL LEYRASLLAG KGFAVMALAY YNYDDLPKTM
210 220 230 240 250
ETMRIEYFEE AVNYLRGHPE VKGPGIGLLG ISKGGELGLA MASFLKGITA
260 270 280 290 300
AVVINGSVAA VGNTICYKDE TIPPVTILRN QVKMTKDGLK DVVDALQSPL
310 320 330 340 350
VEQKSFIPVE RSDTTFLFLV GQDDHNWKSE FYANEISKRL QAHGKEKPQI
360 370 380 390 400
ICYPEAGHYI EPPYFPLCSA GMHLLVGANI TFGGEPKPHS VAQLDAWQQL
410
QTFFHKQLGG KSHGVSPKI
Length:419
Mass (Da):46,013
Last modified:November 1, 1998 - v1
Checksum:i6A0E828A08CEC7C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8E → G (PubMed:9445388).Curated1
Sequence conflicti8E → G (PubMed:9490035).Curated1
Sequence conflicti81I → L (PubMed:9445388).Curated1
Sequence conflicti81I → L (PubMed:9490035).Curated1
Sequence conflicti113E → K (PubMed:9445388).Curated1
Sequence conflicti113E → K (PubMed:9490035).Curated1
Sequence conflicti162D → N (PubMed:9445388).Curated1
Sequence conflicti162D → N (PubMed:9490035).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010428 mRNA. Translation: BAA32434.1.
Y09334 mRNA. Translation: CAA70514.1.
PIRiJE0267.
RefSeqiNP_112605.1. NM_031315.2.
UniGeneiRn.11326.

Genome annotation databases

GeneIDi50559.
KEGGirno:50559.
UCSCiRGD:70894. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010428 mRNA. Translation: BAA32434.1.
Y09334 mRNA. Translation: CAA70514.1.
PIRiJE0267.
RefSeqiNP_112605.1. NM_031315.2.
UniGeneiRn.11326.

3D structure databases

ProteinModelPortaliO88267.
SMRiO88267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013729.

Chemistry databases

SwissLipidsiSLP:000000588.

Protein family/group databases

ESTHERiratno-acot1. Acyl-CoA_Thioesterase.
MEROPSiS09.A53.

PTM databases

iPTMnetiO88267.
PhosphoSitePlusiO88267.

Proteomic databases

PaxDbiO88267.
PRIDEiO88267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi50559.
KEGGirno:50559.
UCSCiRGD:70894. rat.

Organism-specific databases

CTDi641371.
RGDi70894. Acot1.

Phylogenomic databases

eggNOGiENOG410II3X. Eukaryota.
COG1073. LUCA.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiO88267.
KOiK01068.
PhylomeDBiO88267.

Enzyme and pathway databases

BRENDAi3.1.2.2. 5301.
3.1.2.20. 5301.

Miscellaneous databases

PROiO88267.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiACOT1_RAT
AccessioniPrimary (citable) accession number: O88267
Secondary accession number(s): O55161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.