Collagen alpha-1(V) chain precursor

Preferred order of sections

Names and origin

Protein names

Recommended name:
Collagen alpha-1(V) chain

Gene names

Name:

Col5a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1838 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin By similarity. Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter. Ref.3
Subunit structure	Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4 By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed in limbs, vertebrae, heart, brain, liver, intestine, tongue, tail, skin, calvaria, lung and kidney of E18 embryos. Ref.1
Developmental stage	Detected at E11, E12, E14, E16 and E18. Ref.1
Post-translational modification	Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates By similarity. Sulfated on 40% of tyrosines By similarity.
Sequence similarities	Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 laminin G-like domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Collagen Repeat Signal
Ligand	Heparin-binding
PTM	Hydroxylation Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood vessel development Inferred from mutant phenotype PubMed 16492673. Source: MGI cell adhesion Inferred from electronic annotation. Source: Compara collagen biosynthetic process Inferred from electronic annotation. Source: Compara collagen fibril organization Inferred from mutant phenotype PubMed 15383546 PubMed 16492673 PubMed 17029294. Source: MGI extracellular fibril organization Inferred from electronic annotation. Source: Compara eye morphogenesis Inferred from electronic annotation. Source: Compara heart morphogenesis Inferred from genetic interaction PubMed 17029294. Source: MGI integrin biosynthetic process Inferred from electronic annotation. Source: Compara regulation of cellular component organization Inferred from mutant phenotype PubMed 15383546. Source: MGI skin development Inferred from mutant phenotype PubMed 15383546 PubMed 16492673. Source: MGI tendon development Inferred from mutant phenotype PubMed 21467034. Source: MGI wound healing, spreading of epidermal cells Inferred from electronic annotation. Source: Compara
Cellular_component	basement membrane Inferred from direct assay PubMed 14676276. Source: MGI collagen Inferred from direct assay PubMed 15383546 PubMed 17683922. Source: MGI collagen type V Inferred from electronic annotation. Source: Compara
Molecular_function	extracellular matrix structural constituent Inferred from direct assay PubMed 15383546. Source: MGI heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 36

36

Potential

Chain

37 – 1838

1802

Collagen alpha-1(V) chain

PRO_0000041761

Regions

Domain

72 – 244

173

Laminin G-like

Domain

1609 – 1837

229

Fibrillar collagen NC1

Region

231 – 443

213

Nonhelical region By similarity

Region

444 – 558

115

Interrupted collagenous region By similarity

Region

559 – 1570

1012

Triple-helical region By similarity

Region

1571 – 1605

35

Nonhelical region By similarity

Amino acid modifications

Modified residue

234

1

Sulfotyrosine Potential

Modified residue

236

1

Sulfotyrosine Potential

Modified residue

240

1

Sulfotyrosine Potential

Modified residue

262

1

Sulfotyrosine Potential

Modified residue

263

1

Sulfotyrosine Potential

Modified residue

570

1

4-hydroxyproline By similarity

Modified residue

576

1

4-hydroxyproline By similarity

Modified residue

621

1

4-hydroxyproline By similarity

Modified residue

627

1

5-hydroxylysine By similarity

Modified residue

639

1

4-hydroxyproline By similarity

Modified residue

642

1

5-hydroxylysine By similarity

Modified residue

648

1

4-hydroxyproline By similarity

Modified residue

654

1

4-hydroxyproline By similarity

Modified residue

657

1

4-hydroxyproline By similarity

Modified residue

675

1

4-hydroxyproline By similarity

Modified residue

678

1

4-hydroxyproline By similarity

Modified residue

680

1

3-hydroxyproline By similarity

Modified residue

686

1

3-hydroxyproline By similarity

Modified residue

690

1

4-hydroxyproline By similarity

Modified residue

696

1

4-hydroxyproline By similarity

Modified residue

705

1

4-hydroxyproline By similarity

Modified residue

708

1

5-hydroxylysine By similarity

Modified residue

717

1

4-hydroxyproline By similarity

Modified residue

720

1

4-hydroxyproline By similarity

Modified residue

726

1

4-hydroxyproline By similarity

Modified residue

732

1

4-hydroxyproline By similarity

Modified residue

744

1

5-hydroxylysine By similarity

Modified residue

750

1

4-hydroxyproline By similarity

Modified residue

756

1

4-hydroxyproline By similarity

Modified residue

762

1

4-hydroxyproline By similarity

Modified residue

765

1

4-hydroxyproline By similarity

Modified residue

771

1

4-hydroxyproline By similarity

Modified residue

774

1

5-hydroxylysine By similarity

Modified residue

780

1

4-hydroxyproline By similarity

Modified residue

789

1

4-hydroxyproline By similarity

Modified residue

795

1

5-hydroxylysine By similarity

Modified residue

804

1

5-hydroxylysine By similarity

Modified residue

807

1

5-hydroxylysine By similarity

Modified residue

810

1

5-hydroxylysine By similarity

Modified residue

816

1

4-hydroxyproline By similarity

Modified residue

819

1

5-hydroxylysine By similarity

Modified residue

834

1

4-hydroxyproline By similarity

Modified residue

846

1

5-hydroxylysine By similarity

Modified residue

864

1

5-hydroxylysine By similarity

Modified residue

870

1

4-hydroxyproline By similarity

Modified residue

873

1

4-hydroxyproline By similarity

Modified residue

876

1

4-hydroxyproline By similarity

Modified residue

882

1

5-hydroxylysine By similarity

Modified residue

888

1

4-hydroxyproline By similarity

Modified residue

891

1

4-hydroxyproline By similarity

Modified residue

897

1

5-hydroxylysine By similarity

Modified residue

903

1

4-hydroxyproline By similarity

Modified residue

906

1

4-hydroxyproline By similarity

Modified residue

930

1

4-hydroxyproline By similarity

Modified residue

945

1

4-hydroxyproline By similarity

Modified residue

1017

1

4-hydroxyproline By similarity

Modified residue

1020

1

4-hydroxyproline By similarity

Modified residue

1023

1

4-hydroxyproline By similarity

Modified residue

1029

1

4-hydroxyproline By similarity

Modified residue

1221

1

4-hydroxyproline By similarity

Modified residue

1224

1

4-hydroxyproline By similarity

Modified residue

1467

1

4-hydroxyproline By similarity

Modified residue

1470

1

4-hydroxyproline By similarity

Modified residue

1601

1

Sulfotyrosine Potential

Modified residue

1604

1

Sulfotyrosine Potential

Experimental info

Sequence conflict

1097

1

A → S in BAA28786. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O88207 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: D20F4E5198A09ECF
Show »

FASTA

1,838

183,677

        10         20         30         40         50         60 
MDVHTRWKAA RPGALLLSSP LLLFLLLLWA PPSSRAAQPA DLLEMLDFHN LPSGVTKTTG 

        70         80         90        100        110        120 
FCATRRSSSE PDVAYRVSKD AQLSMPTKQL YPESGFPEDF SILTTVKAKK GSQAFLVSIY 

       130        140        150        160        170        180 
NEQGIQQLGL ELGRSPVFLY EDHTGKPGPE EYPLFPGINL SDGKWHRIAL SVYKKNVTLI 

       190        200        210        220        230        240 
LDCKKKITKF LSRSDHPIID TNGIVMFGSR ILDDEIFEGD IQQLLFVSDN RAAYDYCEHY 

       250        260        270        280        290        300 
SPDCDTAVPD TPQSQDPNPD EYYPEGEGET YYYEYPYYED PEDPGKEPAP TQKPVEAARE 

       310        320        330        340        350        360 
TTEVPEEQTQ PLPEAPTVPE TSDTADKEDS LGIGDYDYVP PDDYYTPPPY EDFGYGEGVE 

       370        380        390        400        410        420 
NPDQPTNPDS GAEVPTSTTV TSNTSNPAPG EGKDDLGGEF TEETIKNLEE NYYDPYFDPD 

       430        440        450        460        470        480 
SDSSVSPSEI GPGMPANQDT IFEGIGGPRG EKGQKGEPAI IEPGMLIEGP PGPEGPAGLP 

       490        500        510        520        530        540 
GPPGTTGPTG QMGDPGERGP PGRPGLPGAD GLPGPPGTML MLPFRFGGGG DAGSKGPMVS 

       550        560        570        580        590        600 
AQESQAQAIL QQARLALRGP AGPMGLTGRP GPMGPPGSGG LKGEPGDMGP QGPRGVQGPP 

       610        620        630        640        650        660 
GPTGKPGRRG RAGSDGARGM PGQTGPKGDR GFDGLAGLPG EKGHRGDPGP SGPPGIPGDD 

       670        680        690        700        710        720 
GERGDDGEVG PRGLPGEPGP RGLLGPKGPP GPPGPPGVTG MDGQPGPKGN VGPQGEPGPP 

       730        740        750        760        770        780 
GQQGNPGAQG LPGPQGAIGP PGEKGPLGKP GLPGMPGADG PPGHPGKEGP PGEKGGQGPP 

       790        800        810        820        830        840 
GPQGPIGYPG PRGVKGADGI RGLKGTKGEK GEDGFPGFKG DMGIKGDRGE IGPPGPRGED 

       850        860        870        880        890        900 
GPEGPKGRGG PNGDPGPLGP TGEKGKLGVP GLPGYPGRQG PKGSIGFPGF PGANGEKGGR 

       910        920        930        940        950        960 
GTPGKPGPRG QRGPTGPRGE RGPRGITGKP GPKGNSGGDG PAGPPGERGP NGPQGPTGFP 

       970        980        990       1000       1010       1020 
GPKGPPGPPG KDGLPGHPGQ RGETGFQGKT GPPGPPGVVG PQGPTGETGP MGERGHPGPP 

      1030       1040       1050       1060       1070       1080 
GPPGEQGLPG AAGKEGTKGD PGPAGLPGKD GPPGLRGFPG DRGLPGPVGA LGLKGSEGPP 

      1090       1100       1110       1120       1130       1140 
GPPGPAGSPG ERGPAGAAGP IGIPGRPGPQ GPPGPAGEKG LPGEKGPQGP AGRDGLQGPV 

      1150       1160       1170       1180       1190       1200 
GLPGPAGPVG PPGEDGDKGE IGEPGQKGSK GDKGEQGPPG PTGPQGPIGQ PGPSGADGEP 

      1210       1220       1230       1240       1250       1260 
GPRGQQGLFG QKGDEGSRGF PGPPGPVGLQ GLPGPPGEKG ETGDVGQMGP PGPPGPRGPS 

      1270       1280       1290       1300       1310       1320 
GAPGADGPQG PPGGIGNPGA VGEKGEPGEA GDPGLPGEGG PLGPKGERGE KGEAGPSGAA 

      1330       1340       1350       1360       1370       1380 
GPPGPKGPPG DDGPKGSPGP VGFPGDPGPP GEPGPAGQDG PPGDKGDDGE PGQTGSPGPT 

      1390       1400       1410       1420       1430       1440 
GEPGPSGPPG KRGPPGPAGP EGRQGEKGAK GEAGLEGPPG KTGPIGPQGA PGKPGPDGLR 

      1450       1460       1470       1480       1490       1500 
GIPGPVGEQG LPGSPGPDGP PGPMGPPGLP GLKGDSGPKG EKGHPGLIGL IGPPGEQGEK 

      1510       1520       1530       1540       1550       1560 
GDRGLPGPQG SSGPKGDQGI TGPSGPLGPP GPPGLPGPPG PKGAKGSSGP TGPKGEAGHP 

      1570       1580       1590       1600       1610       1620 
GLPGPPGPPG EVIQPLPIQA SRTRRNIDAS QLLDDGAGES YVDYADGMEE IFGSLNSLKL 

      1630       1640       1650       1660       1670       1680 
EIEQMKRPLG TQQNPARTCK DLQLCHPDFP DGEYWVDPNQ GCSRDSFKVY CNFTAGGSTC 

      1690       1700       1710       1720       1730       1740 
VFPDKKSEGA RITSWPKENP GSWFSEFKRG KLLSYVDAEG NPVGVVQMTF LRLLSASAHQ 

      1750       1760       1770       1780       1790       1800 
NVTYNCYQSV AWQDAATGSY DKAIRFLGSN DEEMSYDNNP YIRALVDGCA TKKGYQKTVL 

      1810       1820       1830 
EIDTPKVEQV PIVDIMFNDF GEASQKFGFE VGPACFLG

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence and expression of the mouse alpha1(V) collagen gene." Wu Y.L., Sumiyoshi H., Khaleduzzaman M., Ninomiya Y., Yoshioka H. Biochim. Biophys. Acta 1397:275-284(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Identification of a functional CBF-binding CCAAT-like motif in the core promoter of the mouse pro-alpha1(V) collagen gene (Col5a1)." Sakata-Takatani K., Matsuo N., Sumiyoshi H., Tsuda T., Yoshioka H. Matrix Biol. 23:87-99(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30, FUNCTION.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1156-1838. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB009993 mRNA. Translation: BAA28786.1. AL731778, AL732513 Genomic DNA. Translation: CAM46247.1. AB098608 Genomic DNA. Translation: BAD26732.1. AK148055 mRNA. Translation: BAE28315.1.
IPI	IPI00128689.
RefSeq	NP_056549.2. NM_015734.2.
UniGene	Mm.7281.
3D structure databases
ProteinModelPortal	O88207.
SMR	O88207. Positions 63-208, 1628-1837.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88207. 1 interaction.
PTM databases
PhosphoSite	O88207.
Proteomic databases
PaxDb	O88207.
PRIDE	O88207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028280; ENSMUSP00000028280; ENSMUSG00000026837.
GeneID	12831.
KEGG	mmu:12831.
Organism-specific databases
CTD	1289.
MGI	MGI:88457. Col5a1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104155.
HOGENOM	HOG000085654.
HOVERGEN	HBG004933.
InParanoid	O88207.
KO	K06236.
OMA	TIYEGIG.
OrthoDB	EOG49GKHM.
Gene expression databases
ArrayExpress	O88207.
Bgee	O88207.
CleanEx	MM_COL5A1.
Genevestigator	O88207.
GermOnline	ENSMUSG00000026837. Mus musculus.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008160. Collagen. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000885. Fib_collagen_C. IPR001791. Laminin_G. [Graphical view]
Pfam	PF01410. COLFI. 1 hit. PF01391. Collagen. 6 hits. PF02210. Laminin_G_2. 1 hit. [Graphical view]
ProDom	PD002078. Fib_collagen_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00038. COLFI. 1 hit. SM00282. LamG. 1 hit. SM00210. TSPN. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS50025. LAM_G_DOMAIN. False negative. PS51461. NC1_FIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	282334.
SOURCE	Search...

Entry information

Entry name

CO5A1_MOUSE

Accession

Primary (citable) accession number: O88207
Secondary accession number(s): A3KGE3, Q3UG94, Q6F6B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents