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O88207 (CO5A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Collagen alpha-1(V) chain
Gene names
Name:Col5a1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1838 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin By similarity. Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter. Ref.3

Subunit structure

Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4 By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Expressed in limbs, vertebrae, heart, brain, liver, intestine, tongue, tail, skin, calvaria, lung and kidney of E18 embryos. Ref.1

Developmental stage

Detected at E11, E12, E14, E16 and E18. Ref.1

Post-translational modification

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates By similarity.

Sulfated on 40% of tyrosines By similarity.

Sequence similarities

Belongs to the fibrillar collagen family.

Contains 1 fibrillar collagen NC1 domain.

Contains 1 laminin G-like domain.

Ontologies

Keywords
   Cellular componentExtracellular matrix
Secreted
   DomainCollagen
Repeat
Signal
   LigandHeparin-binding
   PTMHydroxylation
Sulfation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood vessel development

Inferred from mutant phenotype PubMed 16492673. Source: MGI

cell adhesion

Inferred from electronic annotation. Source: Ensembl

collagen biosynthetic process

Inferred from electronic annotation. Source: Ensembl

collagen fibril organization

Inferred from mutant phenotype PubMed 15383546PubMed 16492673PubMed 17029294. Source: MGI

extracellular fibril organization

Inferred from electronic annotation. Source: Ensembl

eye morphogenesis

Inferred from electronic annotation. Source: Ensembl

heart morphogenesis

Inferred from genetic interaction PubMed 17029294. Source: MGI

integrin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

regulation of cellular component organization

Inferred from mutant phenotype PubMed 15383546. Source: MGI

skin development

Inferred from mutant phenotype PubMed 15383546PubMed 16492673. Source: MGI

tendon development

Inferred from mutant phenotype PubMed 21467034. Source: MGI

wound healing, spreading of epidermal cells

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentbasement membrane

Inferred from direct assay PubMed 14676276. Source: MGI

collagen

Inferred from direct assay PubMed 15383546PubMed 17683922. Source: MGI

collagen type V

Inferred from electronic annotation. Source: Ensembl

extracellular matrix

Inferred from direct assay PubMed 17029294. Source: MGI

   Molecular_functionextracellular matrix structural constituent

Inferred from direct assay PubMed 15383546. Source: MGI

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636 Potential
Chain37 – 18381802Collagen alpha-1(V) chain
PRO_0000041761

Regions

Domain72 – 244173Laminin G-like
Domain1609 – 1837229Fibrillar collagen NC1
Region231 – 443213Nonhelical region By similarity
Region444 – 558115Interrupted collagenous region By similarity
Region559 – 15701012Triple-helical region By similarity
Region1571 – 160535Nonhelical region By similarity

Amino acid modifications

Modified residue2341Sulfotyrosine Potential
Modified residue2361Sulfotyrosine Potential
Modified residue2401Sulfotyrosine Potential
Modified residue2621Sulfotyrosine Potential
Modified residue2631Sulfotyrosine Potential
Modified residue57014-hydroxyproline By similarity
Modified residue57614-hydroxyproline By similarity
Modified residue62114-hydroxyproline By similarity
Modified residue62715-hydroxylysine By similarity
Modified residue63914-hydroxyproline By similarity
Modified residue64215-hydroxylysine By similarity
Modified residue64814-hydroxyproline By similarity
Modified residue65414-hydroxyproline By similarity
Modified residue65714-hydroxyproline By similarity
Modified residue67514-hydroxyproline By similarity
Modified residue67814-hydroxyproline By similarity
Modified residue68013-hydroxyproline By similarity
Modified residue68613-hydroxyproline By similarity
Modified residue69014-hydroxyproline By similarity
Modified residue69614-hydroxyproline By similarity
Modified residue70514-hydroxyproline By similarity
Modified residue70815-hydroxylysine By similarity
Modified residue71714-hydroxyproline By similarity
Modified residue72014-hydroxyproline By similarity
Modified residue72614-hydroxyproline By similarity
Modified residue73214-hydroxyproline By similarity
Modified residue74415-hydroxylysine By similarity
Modified residue75014-hydroxyproline By similarity
Modified residue75614-hydroxyproline By similarity
Modified residue76214-hydroxyproline By similarity
Modified residue76514-hydroxyproline By similarity
Modified residue77114-hydroxyproline By similarity
Modified residue77415-hydroxylysine By similarity
Modified residue78014-hydroxyproline By similarity
Modified residue78914-hydroxyproline By similarity
Modified residue79515-hydroxylysine By similarity
Modified residue80415-hydroxylysine By similarity
Modified residue80715-hydroxylysine By similarity
Modified residue81015-hydroxylysine By similarity
Modified residue81614-hydroxyproline By similarity
Modified residue81915-hydroxylysine By similarity
Modified residue83414-hydroxyproline By similarity
Modified residue84615-hydroxylysine By similarity
Modified residue86415-hydroxylysine By similarity
Modified residue87014-hydroxyproline By similarity
Modified residue87314-hydroxyproline By similarity
Modified residue87614-hydroxyproline By similarity
Modified residue88215-hydroxylysine By similarity
Modified residue88814-hydroxyproline By similarity
Modified residue89114-hydroxyproline By similarity
Modified residue89715-hydroxylysine By similarity
Modified residue90314-hydroxyproline By similarity
Modified residue90614-hydroxyproline By similarity
Modified residue93014-hydroxyproline By similarity
Modified residue94514-hydroxyproline By similarity
Modified residue101714-hydroxyproline By similarity
Modified residue102014-hydroxyproline By similarity
Modified residue102314-hydroxyproline By similarity
Modified residue102914-hydroxyproline By similarity
Modified residue122114-hydroxyproline By similarity
Modified residue122414-hydroxyproline By similarity
Modified residue146714-hydroxyproline By similarity
Modified residue147014-hydroxyproline By similarity
Modified residue16011Sulfotyrosine Potential
Modified residue16041Sulfotyrosine Potential

Experimental info

Sequence conflict10971A → S in BAA28786. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O88207 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: D20F4E5198A09ECF

FASTA1,838183,677
        10         20         30         40         50         60 
MDVHTRWKAA RPGALLLSSP LLLFLLLLWA PPSSRAAQPA DLLEMLDFHN LPSGVTKTTG 

        70         80         90        100        110        120 
FCATRRSSSE PDVAYRVSKD AQLSMPTKQL YPESGFPEDF SILTTVKAKK GSQAFLVSIY 

       130        140        150        160        170        180 
NEQGIQQLGL ELGRSPVFLY EDHTGKPGPE EYPLFPGINL SDGKWHRIAL SVYKKNVTLI 

       190        200        210        220        230        240 
LDCKKKITKF LSRSDHPIID TNGIVMFGSR ILDDEIFEGD IQQLLFVSDN RAAYDYCEHY 

       250        260        270        280        290        300 
SPDCDTAVPD TPQSQDPNPD EYYPEGEGET YYYEYPYYED PEDPGKEPAP TQKPVEAARE 

       310        320        330        340        350        360 
TTEVPEEQTQ PLPEAPTVPE TSDTADKEDS LGIGDYDYVP PDDYYTPPPY EDFGYGEGVE 

       370        380        390        400        410        420 
NPDQPTNPDS GAEVPTSTTV TSNTSNPAPG EGKDDLGGEF TEETIKNLEE NYYDPYFDPD 

       430        440        450        460        470        480 
SDSSVSPSEI GPGMPANQDT IFEGIGGPRG EKGQKGEPAI IEPGMLIEGP PGPEGPAGLP 

       490        500        510        520        530        540 
GPPGTTGPTG QMGDPGERGP PGRPGLPGAD GLPGPPGTML MLPFRFGGGG DAGSKGPMVS 

       550        560        570        580        590        600 
AQESQAQAIL QQARLALRGP AGPMGLTGRP GPMGPPGSGG LKGEPGDMGP QGPRGVQGPP 

       610        620        630        640        650        660 
GPTGKPGRRG RAGSDGARGM PGQTGPKGDR GFDGLAGLPG EKGHRGDPGP SGPPGIPGDD 

       670        680        690        700        710        720 
GERGDDGEVG PRGLPGEPGP RGLLGPKGPP GPPGPPGVTG MDGQPGPKGN VGPQGEPGPP 

       730        740        750        760        770        780 
GQQGNPGAQG LPGPQGAIGP PGEKGPLGKP GLPGMPGADG PPGHPGKEGP PGEKGGQGPP 

       790        800        810        820        830        840 
GPQGPIGYPG PRGVKGADGI RGLKGTKGEK GEDGFPGFKG DMGIKGDRGE IGPPGPRGED 

       850        860        870        880        890        900 
GPEGPKGRGG PNGDPGPLGP TGEKGKLGVP GLPGYPGRQG PKGSIGFPGF PGANGEKGGR 

       910        920        930        940        950        960 
GTPGKPGPRG QRGPTGPRGE RGPRGITGKP GPKGNSGGDG PAGPPGERGP NGPQGPTGFP 

       970        980        990       1000       1010       1020 
GPKGPPGPPG KDGLPGHPGQ RGETGFQGKT GPPGPPGVVG PQGPTGETGP MGERGHPGPP 

      1030       1040       1050       1060       1070       1080 
GPPGEQGLPG AAGKEGTKGD PGPAGLPGKD GPPGLRGFPG DRGLPGPVGA LGLKGSEGPP 

      1090       1100       1110       1120       1130       1140 
GPPGPAGSPG ERGPAGAAGP IGIPGRPGPQ GPPGPAGEKG LPGEKGPQGP AGRDGLQGPV 

      1150       1160       1170       1180       1190       1200 
GLPGPAGPVG PPGEDGDKGE IGEPGQKGSK GDKGEQGPPG PTGPQGPIGQ PGPSGADGEP 

      1210       1220       1230       1240       1250       1260 
GPRGQQGLFG QKGDEGSRGF PGPPGPVGLQ GLPGPPGEKG ETGDVGQMGP PGPPGPRGPS 

      1270       1280       1290       1300       1310       1320 
GAPGADGPQG PPGGIGNPGA VGEKGEPGEA GDPGLPGEGG PLGPKGERGE KGEAGPSGAA 

      1330       1340       1350       1360       1370       1380 
GPPGPKGPPG DDGPKGSPGP VGFPGDPGPP GEPGPAGQDG PPGDKGDDGE PGQTGSPGPT 

      1390       1400       1410       1420       1430       1440 
GEPGPSGPPG KRGPPGPAGP EGRQGEKGAK GEAGLEGPPG KTGPIGPQGA PGKPGPDGLR 

      1450       1460       1470       1480       1490       1500 
GIPGPVGEQG LPGSPGPDGP PGPMGPPGLP GLKGDSGPKG EKGHPGLIGL IGPPGEQGEK 

      1510       1520       1530       1540       1550       1560 
GDRGLPGPQG SSGPKGDQGI TGPSGPLGPP GPPGLPGPPG PKGAKGSSGP TGPKGEAGHP 

      1570       1580       1590       1600       1610       1620 
GLPGPPGPPG EVIQPLPIQA SRTRRNIDAS QLLDDGAGES YVDYADGMEE IFGSLNSLKL 

      1630       1640       1650       1660       1670       1680 
EIEQMKRPLG TQQNPARTCK DLQLCHPDFP DGEYWVDPNQ GCSRDSFKVY CNFTAGGSTC 

      1690       1700       1710       1720       1730       1740 
VFPDKKSEGA RITSWPKENP GSWFSEFKRG KLLSYVDAEG NPVGVVQMTF LRLLSASAHQ 

      1750       1760       1770       1780       1790       1800 
NVTYNCYQSV AWQDAATGSY DKAIRFLGSN DEEMSYDNNP YIRALVDGCA TKKGYQKTVL 

      1810       1820       1830 
EIDTPKVEQV PIVDIMFNDF GEASQKFGFE VGPACFLG 

« Hide

References

« Hide 'large scale' references
[1]"cDNA sequence and expression of the mouse alpha1(V) collagen gene."
Wu Y.L., Sumiyoshi H., Khaleduzzaman M., Ninomiya Y., Yoshioka H.
Biochim. Biophys. Acta 1397:275-284(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Identification of a functional CBF-binding CCAAT-like motif in the core promoter of the mouse pro-alpha1(V) collagen gene (Col5a1)."
Sakata-Takatani K., Matsuo N., Sumiyoshi H., Tsuda T., Yoshioka H.
Matrix Biol. 23:87-99(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30, FUNCTION.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1156-1838.
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB009993 mRNA. Translation: BAA28786.1.
AL731778, AL732513 Genomic DNA. Translation: CAM46247.1.
AB098608 Genomic DNA. Translation: BAD26732.1.
AK148055 mRNA. Translation: BAE28315.1.
RefSeqNP_056549.2. NM_015734.2.
UniGeneMm.7281.

3D structure databases

ProteinModelPortalO88207.
SMRO88207. Positions 38-240, 1628-1837.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198821. 5 interactions.
IntActO88207. 1 interaction.

PTM databases

PhosphoSiteO88207.

Proteomic databases

PaxDbO88207.
PRIDEO88207.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028280; ENSMUSP00000028280; ENSMUSG00000026837.
GeneID12831.
KEGGmmu:12831.
UCSCuc008ixt.2. mouse.

Organism-specific databases

CTD1289.
MGIMGI:88457. Col5a1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00710000106385.
HOGENOMHOG000085654.
HOVERGENHBG004933.
InParanoidO88207.
KOK06236.
OMAPIGVVQM.
OrthoDBEOG7XPZ4W.
PhylomeDBO88207.
TreeFamTF323987.

Gene expression databases

ArrayExpressO88207.
BgeeO88207.
CleanExMM_COL5A1.
GenevestigatorO88207.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008160. Collagen.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000885. Fib_collagen_C.
IPR001791. Laminin_G.
[Graphical view]
PfamPF01410. COLFI. 1 hit.
PF01391. Collagen. 6 hits.
PF02210. Laminin_G_2. 1 hit.
[Graphical view]
ProDomPD002078. Fib_collagen_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00038. COLFI. 1 hit.
SM00282. LamG. 1 hit.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS51461. NC1_FIB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio282334.
PROO88207.
SOURCESearch...

Entry information

Entry nameCO5A1_MOUSE
AccessionPrimary (citable) accession number: O88207
Secondary accession number(s): A3KGE3, Q3UG94, Q6F6B0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot