Reviewed, UniProtKB/Swiss-Prot O88202 (LPP60_RAT)

Names and origin

Protein names

Recommended name:
    60 kDa lysophospholipase
    EC=3.1.1.5
Including the following 2 domains:
    1- Recommended name:
            L-asparaginase
              EC=3.5.1.1
        Alternative name(s):
            L-asparagine amidohydrolase
    2- Recommended name:
            Platelet-activating factor acetylhydrolase
                Short name=PAF acetylhydrolase
              EC=3.1.1.47

Gene names

Name:

Aspg

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities. Ref.1 Ref.3
Catalytic activity	2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate. Ref.1 L-asparagine + H2O = L-aspartate + NH3. Ref.1 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate. Ref.1
Enzyme regulation	Inhibited by phosphatidic acid. Ref.3
Subunit structure	Monomer. Ref.3
Tissue specificity	Expressed at high levels in liver and kidney and at a low level in stomach. Barely detectable in lung, heart, spleen and brain. Ref.1
Sequence similarities	In the N-terminal section; belongs to the asparaginase 1 family. Contains 5 ANK repeats.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	ANK repeat Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	asparagine metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Ref.1 Ref.3 Inferred from direct assay. Source: UniProtKB
Cellular component	soluble fraction Ref.3 Inferred from direct assay. Source: RGD
Molecular function	1-alkyl-2-acetylglycerophosphocholine esterase activity Ref.1 Inferred from mutant phenotype. Source: RGD asparaginase activity Ref.1 Inferred from direct assay. Source: UniProtKB lysophospholipase activity Ref.1 Ref.3 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	60 kDa lysophospholipase		PRO_0000171092
Regions
	Repeat	141 – 170	30	ANK 1
	Repeat	396 – 426	31	ANK 2
	Repeat	430 – 459	30	ANK 3
	Repeat	463 – 492	30	ANK 4
	Repeat	530 – 559	30	ANK 5
	Region	41 – 350	310	Asparaginase
Sites
	Active site	19	1	By similarity UniProtKB P06608
	Active site	116	1	By similarity UniProtKB P06608
	Active site	117	1	By similarity UniProtKB P06608
	Active site	187	1	By similarity UniProtKB P06608
Amino acid modifications
	Modified residue	387	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	280	1	I → L in AAH98655. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O88202-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 9DBB2F8FA8B1C477	FASTA	564	60,795
	10 20 30 40 50 60 MARATGPEQR LLAIYTGGTI GMRSEGGVLV PGRGLAAVLR TLHMLHDEEY ARAHSLPEDT 70 80 90 100 110 120 LVLPPASSDQ RIIYKVLECQ PLFDSSDMTI TEWVQIAQTI ERHYTQYQGF VVIHGTDTMA 130 140 150 160 170 180 FAASVLSFML ENLQKPVILT GAQVPIHELW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 190 200 210 220 230 240 FRGNRTTKVD ARRFAAFCSP NLPPLATVGA DVTINRELVR KASWKSHLVV HSNMEPDVGL 250 260 270 280 290 300 LRLYPGIPAS LVRTFLQPPL KGVVMETFGS GNGPTKPDLI QELRAAAERG LIIVNCTHCL 310 320 330 340 350 360 QGAVTSDYAP GMAMAGAGII SGFDMTSEAA LAKLSYVLGQ PGLSLSDRKK LLAKDLRGEM 370 380 390 400 410 420 TLPTTDDLLG DDMLGCRATW LLSMNGSQDA DAMKDVLLPG LALAAAHAGD LDTLQAFVEL 430 440 450 460 470 480 GRDLNLKDYS GQTPLHVAAR RGHASVVAML LQKGVDVDAC NEDGQSPLLL AVRGRHQSVI 490 500 510 520 530 540 RLLRAAGAHL SPQELEDVGT ELCRLASRAD SEGLRAWWQA GADLGQPDYD GHCALQVAEA 550 560 AGNADVVALL QSLEDRVSAQ PQPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of cDNA encoding rat liver 60-kDa lysophospholipase containing an asparaginase-like region and ankyrin repeat." Sugimoto H., Odani S., Yamashita S. J. Biol. Chem. 273:12536-12542(1998) [PubMed: 9575212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 226-233 AND 428-440, TISSUE SPECIFICITY, ENZYME ACTIVITY. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[3]	"Purification, characterization, and inhibition by phosphatidic acid of lysophospholipase transacylase from rat liver." Sugimoto H., Yamashita S. J. Biol. Chem. 269:6252-6258(1994) [PubMed: 8119970] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB009372 mRNA. Translation: BAA31197.1. BC098655 mRNA. Translation: AAH98655.1.
IPI	IPI00207299.
RefSeq	NP_653351.1.
UniGene	Rn.29979
3D structure databases
HSSP	HSSP built from PDB template 1SW6 based on UniProtKB P09959.
ModBase	Search...
PTM databases
PhosphoSite	O88202.
Proteomic databases
PRIDE	O88202.
Genome annotation databases
Ensembl	ENSRNOG00000012843. Rattus norvegicus. [Contig view]
GeneID	246266.
KEGG	rno:246266.
Organism-specific databases
RGD	708388. LOC246266.
Phylogenomic databases
HOVERGEN	O88202.
Enzyme and pathway databases
BRENDA	3.1.1.47. 248. 3.1.1.5. 248. 3.5.1.1. 248.
Gene expression databases
ArrayExpress	O88202.
GermOnline	ENSRNOG00000012843. Rattus norvegicus.
Family and domain databases
InterPro	IPR002110. ANK. IPR006033. AsnASEI. IPR006034. Asp/Glutamnse. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00023. Ank. 4 hits. PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
ProDom	PD003221. Asp/Glutamnse. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00248. ANK. 4 hits. [Graphical view]
TIGRFAMs	TIGR00519. asnASE_I. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS00144. ASN_GLN_ASE_1. False negative. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	623626.

Entry information

Entry name

LPP60_RAT

Accession

Primary (citable) accession number: O88202
Secondary accession number(s): Q4G088

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)