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Reviewed, UniProtKB/Swiss-Prot O88202 (LPP60_RAT)

Last modified June 16, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    60 kDa lysophospholipase
    EC=3.1.1.5
Including the following 2 domains:
    1- Recommended name:
            L-asparaginase
              EC=3.5.1.1
        Alternative name(s):
            L-asparagine amidohydrolase
    2- Recommended name:
            Platelet-activating factor acetylhydrolase
                Short name=PAF acetylhydrolase
              EC=3.1.1.47
Gene names
Name: Aspg
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities. Ref.1 Ref.3

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate. Ref.1

L-asparagine + H2O = L-aspartate + NH3. Ref.1

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate. Ref.1

Enzyme regulation

Inhibited by phosphatidic acid. Ref.3

Subunit structure

Monomer. Ref.3

Tissue specificity

Expressed at high levels in liver and kidney and at a low level in stomach. Barely detectable in lung, heart, spleen and brain. Ref.1

Sequence similarities

In the N-terminal section; belongs to the asparaginase 1 family.

Contains 5 ANK repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 56456460 kDa lysophospholipase
PRO_0000171092

Regions

Repeat141 – 17030ANK 1
Repeat396 – 42631ANK 2
Repeat430 – 45930ANK 3
Repeat463 – 49230ANK 4
Repeat530 – 55930ANK 5
Region41 – 350310Asparaginase

Sites

Active site191 By similarity UniProtKB P06608
Active site1161 By similarity UniProtKB P06608
Active site1171 By similarity UniProtKB P06608
Active site1871 By similarity UniProtKB P06608

Amino acid modifications

Modified residue3871Phosphoserine By similarity

Experimental info

Sequence conflict2801I → L in AAH98655. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O88202-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 9DBB2F8FA8B1C477

FASTA56460,795
        10         20         30         40         50         60 
MARATGPEQR LLAIYTGGTI GMRSEGGVLV PGRGLAAVLR TLHMLHDEEY ARAHSLPEDT 

        70         80         90        100        110        120 
LVLPPASSDQ RIIYKVLECQ PLFDSSDMTI TEWVQIAQTI ERHYTQYQGF VVIHGTDTMA 

       130        140        150        160        170        180 
FAASVLSFML ENLQKPVILT GAQVPIHELW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 

       190        200        210        220        230        240 
FRGNRTTKVD ARRFAAFCSP NLPPLATVGA DVTINRELVR KASWKSHLVV HSNMEPDVGL 

       250        260        270        280        290        300 
LRLYPGIPAS LVRTFLQPPL KGVVMETFGS GNGPTKPDLI QELRAAAERG LIIVNCTHCL 

       310        320        330        340        350        360 
QGAVTSDYAP GMAMAGAGII SGFDMTSEAA LAKLSYVLGQ PGLSLSDRKK LLAKDLRGEM 

       370        380        390        400        410        420 
TLPTTDDLLG DDMLGCRATW LLSMNGSQDA DAMKDVLLPG LALAAAHAGD LDTLQAFVEL 

       430        440        450        460        470        480 
GRDLNLKDYS GQTPLHVAAR RGHASVVAML LQKGVDVDAC NEDGQSPLLL AVRGRHQSVI 

       490        500        510        520        530        540 
RLLRAAGAHL SPQELEDVGT ELCRLASRAD SEGLRAWWQA GADLGQPDYD GHCALQVAEA 

       550        560 
AGNADVVALL QSLEDRVSAQ PQPH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of cDNA encoding rat liver 60-kDa lysophospholipase containing an asparaginase-like region and ankyrin repeat."
Sugimoto H., Odani S., Yamashita S.
J. Biol. Chem. 273:12536-12542(1998) [PubMed: 9575212] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 226-233 AND 428-440, TISSUE SPECIFICITY, ENZYME ACTIVITY.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[3]"Purification, characterization, and inhibition by phosphatidic acid of lysophospholipase transacylase from rat liver."
Sugimoto H., Yamashita S.
J. Biol. Chem. 269:6252-6258(1994) [PubMed: 8119970] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

AB009372 mRNA. Translation: BAA31197.1.
BC098655 mRNA. Translation: AAH98655.1.
IPIIPI00207299.
RefSeqNP_653351.1.
UniGeneRn.29979

3D structure databases

HSSPHSSP built from PDB template 1SW6 based on UniProtKB P09959.
ModBaseSearch...

PTM databases

PhosphoSiteO88202.

Proteomic databases

PRIDEO88202.

Genome annotation databases

EnsemblENSRNOG00000012843. Rattus norvegicus. [Contig view]
GeneID246266.
KEGGrno:246266.

Organism-specific databases

RGD708388. LOC246266.

Phylogenomic databases

HOVERGENO88202.

Enzyme and pathway databases

BRENDA3.1.1.47. 248.
3.1.1.5. 248.
3.5.1.1. 248.

Gene expression databases

ArrayExpressO88202.
GermOnlineENSRNOG00000012843. Rattus norvegicus.

Family and domain databases

InterProIPR002110. ANK.
IPR006033. AsnASEI.
IPR006034. Asp/Glutamnse.
[Graphical view]
Gene3DG3DSA:1.25.40.20. ANK. 1 hit.
PANTHERPTHR11707. Asp/Glutamnse. 1 hit.
PfamPF00023. Ank. 4 hits.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PRINTSPR00139. ASNGLNASE.
ProDomPD003221. Asp/Glutamnse. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00248. ANK. 4 hits.
[Graphical view]
TIGRFAMsTIGR00519. asnASE_I. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00144. ASN_GLN_ASE_1. False negative.
PS00917. ASN_GLN_ASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio623626.

Entry information

Entry nameLPP60_RAT
AccessionPrimary (citable) accession number: O88202
Secondary accession number(s): Q4G088
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 1998
Last modified: June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents