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O88202 (LPP60_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60 kDa lysophospholipase

EC=3.1.1.5

Including the following 2 domains:

  1. L-asparaginase
    EC=3.5.1.1
    Alternative name(s):
    L-asparagine amidohydrolase
  2. Platelet-activating factor acetylhydrolase
    Short name=PAF acetylhydrolase
    EC=3.1.1.47
Gene names
Name:Aspg
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities. Ref.1 Ref.3

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate. Ref.1

L-asparagine + H2O = L-aspartate + NH3. Ref.1

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate. Ref.1

Enzyme regulation

Inhibited by phosphatidic acid. Ref.3

Subunit structure

Monomer. Ref.3

Tissue specificity

Expressed at high levels in liver and kidney and at a low level in stomach. Barely detectable in lung, heart, spleen and brain. Ref.1

Sequence similarities

In the N-terminal section; belongs to the asparaginase 1 family.

Contains 5 ANK repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 56456460 kDa lysophospholipase
PRO_0000171092

Regions

Repeat141 – 17030ANK 1
Repeat396 – 42631ANK 2
Repeat430 – 45930ANK 3
Repeat463 – 49230ANK 4
Repeat530 – 55930ANK 5
Region41 – 350310Asparaginase
Region84 – 863Substrate binding By similarity
Region116 – 1172Substrate binding By similarity

Sites

Active site191Acyl-ester intermediate By similarity UniProtKB P06608

Experimental info

Sequence conflict2801I → L in AAH98655. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O88202 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 9DBB2F8FA8B1C477

FASTA56460,795
        10         20         30         40         50         60 
MARATGPEQR LLAIYTGGTI GMRSEGGVLV PGRGLAAVLR TLHMLHDEEY ARAHSLPEDT 

        70         80         90        100        110        120 
LVLPPASSDQ RIIYKVLECQ PLFDSSDMTI TEWVQIAQTI ERHYTQYQGF VVIHGTDTMA 

       130        140        150        160        170        180 
FAASVLSFML ENLQKPVILT GAQVPIHELW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 

       190        200        210        220        230        240 
FRGNRTTKVD ARRFAAFCSP NLPPLATVGA DVTINRELVR KASWKSHLVV HSNMEPDVGL 

       250        260        270        280        290        300 
LRLYPGIPAS LVRTFLQPPL KGVVMETFGS GNGPTKPDLI QELRAAAERG LIIVNCTHCL 

       310        320        330        340        350        360 
QGAVTSDYAP GMAMAGAGII SGFDMTSEAA LAKLSYVLGQ PGLSLSDRKK LLAKDLRGEM 

       370        380        390        400        410        420 
TLPTTDDLLG DDMLGCRATW LLSMNGSQDA DAMKDVLLPG LALAAAHAGD LDTLQAFVEL 

       430        440        450        460        470        480 
GRDLNLKDYS GQTPLHVAAR RGHASVVAML LQKGVDVDAC NEDGQSPLLL AVRGRHQSVI 

       490        500        510        520        530        540 
RLLRAAGAHL SPQELEDVGT ELCRLASRAD SEGLRAWWQA GADLGQPDYD GHCALQVAEA 

       550        560 
AGNADVVALL QSLEDRVSAQ PQPH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of cDNA encoding rat liver 60-kDa lysophospholipase containing an asparaginase-like region and ankyrin repeat."
Sugimoto H., Odani S., Yamashita S.
J. Biol. Chem. 273:12536-12542(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 226-233 AND 428-440, TISSUE SPECIFICITY, ENZYME ACTIVITY.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[3]"Purification, characterization, and inhibition by phosphatidic acid of lysophospholipase transacylase from rat liver."
Sugimoto H., Yamashita S.
J. Biol. Chem. 269:6252-6258(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB009372 mRNA. Translation: BAA31197.1.
BC098655 mRNA. Translation: AAH98655.1.
RefSeqNP_653351.1. NM_144750.2.
UniGeneRn.29979.

3D structure databases

ProteinModelPortalO88202.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000017454.

PTM databases

PhosphoSiteO88202.

Proteomic databases

PaxDbO88202.
PRIDEO88202.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000017454; ENSRNOP00000017454; ENSRNOG00000012843.
GeneID246266.
KEGGrno:246266.
UCSCRGD:708388. rat.

Organism-specific databases

CTD374569.
RGD708388. Aspg.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00390000001610.
HOGENOMHOG000227974.
HOVERGENHBG079531.
InParanoidO88202.
KOK13278.
OrthoDBEOG7WDN22.
PhylomeDBO88202.
TreeFamTF315247.

Gene expression databases

GenevestigatorO88202.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamPF00023. Ank. 2 hits.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSPR01415. ANKYRIN.
PR00139. ASNGLNASE.
SMARTSM00248. ANK. 4 hits.
SM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF53774. SSF53774. 1 hit.
TIGRFAMsTIGR00519. asnASE_I. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00917. ASN_GLN_ASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623626.
PROO88202.

Entry information

Entry nameLPP60_RAT
AccessionPrimary (citable) accession number: O88202
Secondary accession number(s): Q4G088
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families