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O88202

- LPP60_RAT

UniProt

O88202 - LPP60_RAT

Protein

60 kDa lysophospholipase

Gene

Aspg

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.2 Publications

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.1 Publication
    L-asparagine + H2O = L-aspartate + NH3.1 Publication
    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.1 Publication

    Enzyme regulationi

    Inhibited by phosphatidic acid.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: RGD
    2. asparaginase activity Source: UniProtKB
    3. lysophospholipase activity Source: UniProtKB

    GO - Biological processi

    1. asparagine metabolic process Source: UniProtKB
    2. lipid catabolic process Source: UniProtKB-KW
    3. phospholipid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60 kDa lysophospholipase (EC:3.1.1.5)
    Including the following 2 domains:
    L-asparaginase (EC:3.5.1.1)
    Alternative name(s):
    L-asparagine amidohydrolase
    Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
    Short name:
    PAF acetylhydrolase
    Gene namesi
    Name:Aspg
    OrganismiRattus norvegicus (Rat)Imported
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi708388. Aspg.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 56456460 kDa lysophospholipasePRO_0000171092Add
    BLAST

    Proteomic databases

    PaxDbiO88202.
    PRIDEiO88202.

    PTM databases

    PhosphoSiteiO88202.

    Expressioni

    Tissue specificityi

    Expressed at high levels in liver and kidney and at a low level in stomach. Barely detectable in lung, heart, spleen and brain.1 Publication

    Gene expression databases

    GenevestigatoriO88202.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000017454.

    Structurei

    3D structure databases

    ProteinModelPortaliO88202.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati141 – 17030ANK 1Add
    BLAST
    Repeati396 – 42631ANK 2Add
    BLAST
    Repeati430 – 45930ANK 3Add
    BLAST
    Repeati463 – 49230ANK 4Add
    BLAST
    Repeati530 – 55930ANK 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 350310AsparaginaseAdd
    BLAST
    Regioni84 – 863Substrate bindingBy similarity
    Regioni116 – 1172Substrate bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the asparaginase 1 family.Curated
    Contains 5 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00390000001610.
    HOGENOMiHOG000227974.
    HOVERGENiHBG079531.
    InParanoidiO88202.
    KOiK13278.
    OrthoDBiEOG7WDN22.
    PhylomeDBiO88202.
    TreeFamiTF315247.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.40.50.1170. 1 hit.
    3.40.50.40. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR006033. AsnASEI.
    IPR006034. Asparaginase/glutaminase.
    IPR027475. Asparaginase/glutaminase_AS2.
    IPR027473. L-asparaginase_C.
    IPR027474. L-asparaginase_N.
    [Graphical view]
    PfamiPF00023. Ank. 2 hits.
    PF00710. Asparaginase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
    PRINTSiPR01415. ANKYRIN.
    PR00139. ASNGLNASE.
    SMARTiSM00248. ANK. 4 hits.
    SM00870. Asparaginase. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF53774. SSF53774. 1 hit.
    TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS00917. ASN_GLN_ASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88202-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARATGPEQR LLAIYTGGTI GMRSEGGVLV PGRGLAAVLR TLHMLHDEEY    50
    ARAHSLPEDT LVLPPASSDQ RIIYKVLECQ PLFDSSDMTI TEWVQIAQTI 100
    ERHYTQYQGF VVIHGTDTMA FAASVLSFML ENLQKPVILT GAQVPIHELW 150
    SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRTTKVD ARRFAAFCSP 200
    NLPPLATVGA DVTINRELVR KASWKSHLVV HSNMEPDVGL LRLYPGIPAS 250
    LVRTFLQPPL KGVVMETFGS GNGPTKPDLI QELRAAAERG LIIVNCTHCL 300
    QGAVTSDYAP GMAMAGAGII SGFDMTSEAA LAKLSYVLGQ PGLSLSDRKK 350
    LLAKDLRGEM TLPTTDDLLG DDMLGCRATW LLSMNGSQDA DAMKDVLLPG 400
    LALAAAHAGD LDTLQAFVEL GRDLNLKDYS GQTPLHVAAR RGHASVVAML 450
    LQKGVDVDAC NEDGQSPLLL AVRGRHQSVI RLLRAAGAHL SPQELEDVGT 500
    ELCRLASRAD SEGLRAWWQA GADLGQPDYD GHCALQVAEA AGNADVVALL 550
    QSLEDRVSAQ PQPH 564
    Length:564
    Mass (Da):60,795
    Last modified:November 1, 1998 - v1
    Checksum:i9DBB2F8FA8B1C477
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti280 – 2801I → L in AAH98655. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009372 mRNA. Translation: BAA31197.1.
    BC098655 mRNA. Translation: AAH98655.1.
    RefSeqiNP_653351.1. NM_144750.2.
    UniGeneiRn.29979.

    Genome annotation databases

    EnsembliENSRNOT00000017454; ENSRNOP00000017454; ENSRNOG00000012843.
    GeneIDi246266.
    KEGGirno:246266.
    UCSCiRGD:708388. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009372 mRNA. Translation: BAA31197.1 .
    BC098655 mRNA. Translation: AAH98655.1 .
    RefSeqi NP_653351.1. NM_144750.2.
    UniGenei Rn.29979.

    3D structure databases

    ProteinModelPortali O88202.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000017454.

    PTM databases

    PhosphoSitei O88202.

    Proteomic databases

    PaxDbi O88202.
    PRIDEi O88202.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000017454 ; ENSRNOP00000017454 ; ENSRNOG00000012843 .
    GeneIDi 246266.
    KEGGi rno:246266.
    UCSCi RGD:708388. rat.

    Organism-specific databases

    CTDi 374569.
    RGDi 708388. Aspg.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00390000001610.
    HOGENOMi HOG000227974.
    HOVERGENi HBG079531.
    InParanoidi O88202.
    KOi K13278.
    OrthoDBi EOG7WDN22.
    PhylomeDBi O88202.
    TreeFami TF315247.

    Miscellaneous databases

    NextBioi 623626.
    PROi O88202.

    Gene expression databases

    Genevestigatori O88202.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    3.40.50.1170. 1 hit.
    3.40.50.40. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR006033. AsnASEI.
    IPR006034. Asparaginase/glutaminase.
    IPR027475. Asparaginase/glutaminase_AS2.
    IPR027473. L-asparaginase_C.
    IPR027474. L-asparaginase_N.
    [Graphical view ]
    Pfami PF00023. Ank. 2 hits.
    PF00710. Asparaginase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001220. L-ASNase_gatD. 1 hit.
    PRINTSi PR01415. ANKYRIN.
    PR00139. ASNGLNASE.
    SMARTi SM00248. ANK. 4 hits.
    SM00870. Asparaginase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF53774. SSF53774. 1 hit.
    TIGRFAMsi TIGR00519. asnASE_I. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS00917. ASN_GLN_ASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of cDNA encoding rat liver 60-kDa lysophospholipase containing an asparaginase-like region and ankyrin repeat."
      Sugimoto H., Odani S., Yamashita S.
      J. Biol. Chem. 273:12536-12542(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 226-233 AND 428-440, TISSUE SPECIFICITY, ENZYME ACTIVITY.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    3. "Purification, characterization, and inhibition by phosphatidic acid of lysophospholipase transacylase from rat liver."
      Sugimoto H., Yamashita S.
      J. Biol. Chem. 269:6252-6258(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiLPP60_RAT
    AccessioniPrimary (citable) accession number: O88202
    Secondary accession number(s): Q4G088
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3