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O88199

- CHST3_MOUSE

UniProt

O88199 - CHST3_MOUSE

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Protein
Carbohydrate sulfotransferase 3
Gene
Chst3, C6st, Gst0
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.2 Publications

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1417PAPS By similarity
Nucleotide bindingi295 – 3039PAPS By similarity

GO - Molecular functioni

  1. chondroitin 6-sulfotransferase activity Source: MGI
  2. proteoglycan sulfotransferase activity Source: MGI

GO - Biological processi

  1. T cell homeostasis Source: MGI
  2. carbohydrate metabolic process Source: UniProtKB-KW
  3. chondroitin sulfate biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BRENDAi2.8.2.17. 3474.
ReactomeiREACT_196514. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 3 (EC:2.8.2.17)
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name:
C6ST-1
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
Short name:
GST-0
Gene namesi
Name:Chst3
Synonyms:C6st, Gst0
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1858224. Chst3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei20 – 3819Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini39 – 472434Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable through adulthood. In their spleen the level of chondroitin 6'-sulfate is almost undetectable. In the spleen of 5-6 week-old mice, the number of CD62L+CD44(low) T-lymphocytes corresponding to naive T-lymphocytes is significantly decreased, whereas those in other secondary lymphoid organs are unchanged.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Carbohydrate sulfotransferase 3
PRO_0000085189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi74 – 741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi96 – 961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi250 – 2501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi413 – 4131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi457 – 4571N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO88199.

PTM databases

PhosphoSiteiO88199.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, lung, eye and stomach. Constitutively expressed at low level during the mid- to late-gestation period. Expressed in the brain in a temporally controlled manner: peaks at 2 weeks after birth in the cerebellum, but at 3 weeks in the cerebrum. Localizes to stromal cells in the bone marrow, and stromal cells in the marginal zone and red pulp of the spleen, but the sense probe did not.1 Publication

Gene expression databases

ArrayExpressiO88199.
BgeeiO88199.
GenevestigatoriO88199.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065010.

Structurei

3D structure databases

ProteinModelPortaliO88199.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO88199.
KOiK01020.
OrthoDBiEOG7RZ5S0.
PhylomeDBiO88199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

O88199-1 [UniParc]FASTAAdd to Basket

« Hide

MEKGLALPQD FRDLVHSLKI RGRYVLFLAF VVIVFIFIEK ENKIISRVSD    50
KLKQIPHFVA DANSTDPALL LSENASLLSL SELDSTFSHL RSRLHNLSLQ 100
LGVEPAMESQ EAGAEKPSQQ AGAGTRRHVL LMATTRTGSS FVGEFFNQQG 150
NIFYLFEPLW HIERTVFFQQ RGASAAGSAL VYRDVLKQLL LCDLYVLEPF 200
ISPPPEDHLT QFLFRRGSSR SLCEDPVCTP FVKKVFEKYH CRNRRCGPLN 250
VTLAGEACRR KDHVALKAVR IRQLEFLQPL VEDPRLDLRV IQLVRDPRAV 300
LASRIVAFAG KYENWKKWLS EGQDQLSEDE VQRLRGNCES IRLSAELGLR 350
QPAWLRGRYM LVRYEDVARR PLQKAREMYS FAGIPLTPQV EDWIQKNTQA 400
TRDSSDVYST QKNSSEQFEK WRFSMPFKLA QVVQAACGPT MHLFGYKLAR 450
DAASLTNRSI SLLEERGTFW VT 472
Length:472
Mass (Da):53,997
Last modified:November 1, 1998 - v1
Checksum:iF021147196D9D339
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008937 mRNA. Translation: BAA29054.1.
AB008938 mRNA. Translation: BAA29055.1.
AB062109 Genomic DNA. Translation: BAB72166.1.
BC055055 mRNA. Translation: AAH55055.1.
RefSeqiNP_058083.2. NM_016803.3.
UniGeneiMm.12866.

Genome annotation databases

EnsembliENSMUST00000068690; ENSMUSP00000065010; ENSMUSG00000057337.
ENSMUST00000167915; ENSMUSP00000131532; ENSMUSG00000057337.
GeneIDi53374.
KEGGimmu:53374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008937 mRNA. Translation: BAA29054.1 .
AB008938 mRNA. Translation: BAA29055.1 .
AB062109 Genomic DNA. Translation: BAB72166.1 .
BC055055 mRNA. Translation: AAH55055.1 .
RefSeqi NP_058083.2. NM_016803.3.
UniGenei Mm.12866.

3D structure databases

ProteinModelPortali O88199.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000065010.

PTM databases

PhosphoSitei O88199.

Proteomic databases

PRIDEi O88199.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068690 ; ENSMUSP00000065010 ; ENSMUSG00000057337 .
ENSMUST00000167915 ; ENSMUSP00000131532 ; ENSMUSG00000057337 .
GeneIDi 53374.
KEGGi mmu:53374.

Organism-specific databases

CTDi 9469.
MGIi MGI:1858224. Chst3.

Phylogenomic databases

eggNOGi NOG80862.
GeneTreei ENSGT00530000062902.
HOGENOMi HOG000261614.
HOVERGENi HBG106811.
InParanoidi O88199.
KOi K01020.
OrthoDBi EOG7RZ5S0.
PhylomeDBi O88199.

Enzyme and pathway databases

BRENDAi 2.8.2.17. 3474.
Reactomei REACT_196514. Chondroitin sulfate biosynthesis.

Miscellaneous databases

NextBioi 310177.
PROi O88199.
SOURCEi Search...

Gene expression databases

ArrayExpressi O88199.
Bgeei O88199.
Genevestigatori O88199.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view ]
Pfami PF00685. Sulfotransfer_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse chondroitin 6-sulfotransferase: molecular cloning, characterization and chromosomal mapping."
    Uchimura K., Kadomatsu K., Fan Q.-W., Muramatsu H., Kurosawa N., Kaname T., Yamamura K., Fukuta M., Habuchi O., Muramatsu T.
    Glycobiology 8:489-496(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Spleen.
  2. "Functional analysis of the chondroitin 6-sulfotransferase gene in relation to lymphocyte subpopulations, brain development, and oversulfated chondroitin sulfates."
    Uchimura K., Kadomatsu K., Nishimura H., Muramatsu H., Nakamura E., Kurosawa N., Habuchi O., El-Fasakhany F.M., Yoshikai Y., Muramatsu T.
    J. Biol. Chem. 277:1443-1450(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, FUNCTION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiCHST3_MOUSE
AccessioniPrimary (citable) accession number: O88199
Secondary accession number(s): Q794I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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