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Protein

Carbohydrate sulfotransferase 3

Gene

Chst3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.2 Publications

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1417PAPSBy similarity
Nucleotide bindingi295 – 3039PAPSBy similarity

GO - Molecular functioni

  1. chondroitin 6-sulfotransferase activity Source: MGI
  2. proteoglycan sulfotransferase activity Source: MGI

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. chondroitin sulfate biosynthetic process Source: MGI
  3. sulfur compound metabolic process Source: MGI
  4. T cell homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BRENDAi2.8.2.17. 3474.
ReactomeiREACT_314166. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 3 (EC:2.8.2.17)
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name:
C6ST-1
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
Short name:
GST-0
Gene namesi
Name:Chst3
Synonyms:C6st, Gst0
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1858224. Chst3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei20 – 3819Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini39 – 472434LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable through adulthood. In their spleen the level of chondroitin 6'-sulfate is almost undetectable. In the spleen of 5-6 week-old mice, the number of CD62L+CD44(low) T-lymphocytes corresponding to naive T-lymphocytes is significantly decreased, whereas those in other secondary lymphoid organs are unchanged.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Carbohydrate sulfotransferase 3PRO_0000085189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi413 – 4131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO88199.

PTM databases

PhosphoSiteiO88199.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, lung, eye and stomach. Constitutively expressed at low level during the mid- to late-gestation period. Expressed in the brain in a temporally controlled manner: peaks at 2 weeks after birth in the cerebellum, but at 3 weeks in the cerebrum. Localizes to stromal cells in the bone marrow, and stromal cells in the marginal zone and red pulp of the spleen, but the sense probe did not.1 Publication

Gene expression databases

BgeeiO88199.
ExpressionAtlasiO88199. baseline and differential.
GenevestigatoriO88199.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065010.

Structurei

3D structure databases

ProteinModelPortaliO88199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO88199.
KOiK01020.
OrthoDBiEOG7RZ5S0.
PhylomeDBiO88199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

O88199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKGLALPQD FRDLVHSLKI RGRYVLFLAF VVIVFIFIEK ENKIISRVSD
60 70 80 90 100
KLKQIPHFVA DANSTDPALL LSENASLLSL SELDSTFSHL RSRLHNLSLQ
110 120 130 140 150
LGVEPAMESQ EAGAEKPSQQ AGAGTRRHVL LMATTRTGSS FVGEFFNQQG
160 170 180 190 200
NIFYLFEPLW HIERTVFFQQ RGASAAGSAL VYRDVLKQLL LCDLYVLEPF
210 220 230 240 250
ISPPPEDHLT QFLFRRGSSR SLCEDPVCTP FVKKVFEKYH CRNRRCGPLN
260 270 280 290 300
VTLAGEACRR KDHVALKAVR IRQLEFLQPL VEDPRLDLRV IQLVRDPRAV
310 320 330 340 350
LASRIVAFAG KYENWKKWLS EGQDQLSEDE VQRLRGNCES IRLSAELGLR
360 370 380 390 400
QPAWLRGRYM LVRYEDVARR PLQKAREMYS FAGIPLTPQV EDWIQKNTQA
410 420 430 440 450
TRDSSDVYST QKNSSEQFEK WRFSMPFKLA QVVQAACGPT MHLFGYKLAR
460 470
DAASLTNRSI SLLEERGTFW VT
Length:472
Mass (Da):53,997
Last modified:October 31, 1998 - v1
Checksum:iF021147196D9D339
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008937 mRNA. Translation: BAA29054.1.
AB008938 mRNA. Translation: BAA29055.1.
AB062109 Genomic DNA. Translation: BAB72166.1.
BC055055 mRNA. Translation: AAH55055.1.
RefSeqiNP_058083.2. NM_016803.3.
UniGeneiMm.12866.

Genome annotation databases

EnsembliENSMUST00000068690; ENSMUSP00000065010; ENSMUSG00000057337.
ENSMUST00000167915; ENSMUSP00000131532; ENSMUSG00000057337.
GeneIDi53374.
KEGGimmu:53374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008937 mRNA. Translation: BAA29054.1.
AB008938 mRNA. Translation: BAA29055.1.
AB062109 Genomic DNA. Translation: BAB72166.1.
BC055055 mRNA. Translation: AAH55055.1.
RefSeqiNP_058083.2. NM_016803.3.
UniGeneiMm.12866.

3D structure databases

ProteinModelPortaliO88199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065010.

PTM databases

PhosphoSiteiO88199.

Proteomic databases

PRIDEiO88199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068690; ENSMUSP00000065010; ENSMUSG00000057337.
ENSMUST00000167915; ENSMUSP00000131532; ENSMUSG00000057337.
GeneIDi53374.
KEGGimmu:53374.

Organism-specific databases

CTDi9469.
MGIiMGI:1858224. Chst3.

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO88199.
KOiK01020.
OrthoDBiEOG7RZ5S0.
PhylomeDBiO88199.

Enzyme and pathway databases

BRENDAi2.8.2.17. 3474.
ReactomeiREACT_314166. Chondroitin sulfate biosynthesis.

Miscellaneous databases

NextBioi310177.
PROiO88199.
SOURCEiSearch...

Gene expression databases

BgeeiO88199.
ExpressionAtlasiO88199. baseline and differential.
GenevestigatoriO88199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse chondroitin 6-sulfotransferase: molecular cloning, characterization and chromosomal mapping."
    Uchimura K., Kadomatsu K., Fan Q.-W., Muramatsu H., Kurosawa N., Kaname T., Yamamura K., Fukuta M., Habuchi O., Muramatsu T.
    Glycobiology 8:489-496(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Spleen.
  2. "Functional analysis of the chondroitin 6-sulfotransferase gene in relation to lymphocyte subpopulations, brain development, and oversulfated chondroitin sulfates."
    Uchimura K., Kadomatsu K., Nishimura H., Muramatsu H., Nakamura E., Kurosawa N., Habuchi O., El-Fasakhany F.M., Yoshikai Y., Muramatsu T.
    J. Biol. Chem. 277:1443-1450(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, FUNCTION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiCHST3_MOUSE
AccessioniPrimary (citable) accession number: O88199
Secondary accession number(s): Q794I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 14, 2005
Last sequence update: October 31, 1998
Last modified: March 31, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.