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Protein

Carbohydrate sulfotransferase 3

Gene

Chst3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.2 Publications

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 141PAPSBy similarity7
Nucleotide bindingi295 – 303PAPSBy similarity9

GO - Molecular functioni

  • chondroitin 6-sulfotransferase activity Source: MGI
  • N-acetylglucosamine 6-O-sulfotransferase activity Source: GO_Central
  • proteoglycan sulfotransferase activity Source: MGI

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • chondroitin sulfate biosynthetic process Source: MGI
  • sulfur compound metabolic process Source: MGI
  • T cell homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BRENDAi2.8.2.17. 3474.
ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 3 (EC:2.8.2.17)
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name:
C6ST-1
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
Short name:
GST-0
Gene namesi
Name:Chst3
Synonyms:C6st, Gst0
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1858224. Chst3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicSequence analysisAdd BLAST19
Transmembranei20 – 38Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini39 – 472LumenalSequence analysisAdd BLAST434

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable through adulthood. In their spleen the level of chondroitin 6'-sulfate is almost undetectable. In the spleen of 5-6 week-old mice, the number of CD62L+CD44(low) T-lymphocytes corresponding to naive T-lymphocytes is significantly decreased, whereas those in other secondary lymphoid organs are unchanged.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851891 – 472Carbohydrate sulfotransferase 3Add BLAST472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi74N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi413N-linked (GlcNAc...)Sequence analysis1
Glycosylationi457N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO88199.
PRIDEiO88199.

PTM databases

PhosphoSitePlusiO88199.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, lung, eye and stomach. Constitutively expressed at low level during the mid- to late-gestation period. Expressed in the brain in a temporally controlled manner: peaks at 2 weeks after birth in the cerebellum, but at 3 weeks in the cerebrum. Localizes to stromal cells in the bone marrow, and stromal cells in the marginal zone and red pulp of the spleen, but the sense probe did not.1 Publication

Gene expression databases

BgeeiENSMUSG00000057337.
ExpressionAtlasiO88199. baseline and differential.
GenevisibleiO88199. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000126281.

Structurei

3D structure databases

ProteinModelPortaliO88199.
SMRiO88199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIAV. Eukaryota.
ENOG410YDA4. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO88199.
KOiK01020.
PhylomeDBiO88199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

O88199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKGLALPQD FRDLVHSLKI RGRYVLFLAF VVIVFIFIEK ENKIISRVSD
60 70 80 90 100
KLKQIPHFVA DANSTDPALL LSENASLLSL SELDSTFSHL RSRLHNLSLQ
110 120 130 140 150
LGVEPAMESQ EAGAEKPSQQ AGAGTRRHVL LMATTRTGSS FVGEFFNQQG
160 170 180 190 200
NIFYLFEPLW HIERTVFFQQ RGASAAGSAL VYRDVLKQLL LCDLYVLEPF
210 220 230 240 250
ISPPPEDHLT QFLFRRGSSR SLCEDPVCTP FVKKVFEKYH CRNRRCGPLN
260 270 280 290 300
VTLAGEACRR KDHVALKAVR IRQLEFLQPL VEDPRLDLRV IQLVRDPRAV
310 320 330 340 350
LASRIVAFAG KYENWKKWLS EGQDQLSEDE VQRLRGNCES IRLSAELGLR
360 370 380 390 400
QPAWLRGRYM LVRYEDVARR PLQKAREMYS FAGIPLTPQV EDWIQKNTQA
410 420 430 440 450
TRDSSDVYST QKNSSEQFEK WRFSMPFKLA QVVQAACGPT MHLFGYKLAR
460 470
DAASLTNRSI SLLEERGTFW VT
Length:472
Mass (Da):53,997
Last modified:November 1, 1998 - v1
Checksum:iF021147196D9D339
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008937 mRNA. Translation: BAA29054.1.
AB008938 mRNA. Translation: BAA29055.1.
AB062109 Genomic DNA. Translation: BAB72166.1.
BC055055 mRNA. Translation: AAH55055.1.
RefSeqiNP_058083.2. NM_016803.3.
UniGeneiMm.12866.

Genome annotation databases

EnsembliENSMUST00000068690; ENSMUSP00000065010; ENSMUSG00000057337.
ENSMUST00000167915; ENSMUSP00000131532; ENSMUSG00000057337.
GeneIDi53374.
KEGGimmu:53374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008937 mRNA. Translation: BAA29054.1.
AB008938 mRNA. Translation: BAA29055.1.
AB062109 Genomic DNA. Translation: BAB72166.1.
BC055055 mRNA. Translation: AAH55055.1.
RefSeqiNP_058083.2. NM_016803.3.
UniGeneiMm.12866.

3D structure databases

ProteinModelPortaliO88199.
SMRiO88199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000126281.

PTM databases

PhosphoSitePlusiO88199.

Proteomic databases

PaxDbiO88199.
PRIDEiO88199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068690; ENSMUSP00000065010; ENSMUSG00000057337.
ENSMUST00000167915; ENSMUSP00000131532; ENSMUSG00000057337.
GeneIDi53374.
KEGGimmu:53374.

Organism-specific databases

CTDi9469.
MGIiMGI:1858224. Chst3.

Phylogenomic databases

eggNOGiENOG410IIAV. Eukaryota.
ENOG410YDA4. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO88199.
KOiK01020.
PhylomeDBiO88199.

Enzyme and pathway databases

BRENDAi2.8.2.17. 3474.
ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

PROiO88199.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057337.
ExpressionAtlasiO88199. baseline and differential.
GenevisibleiO88199. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCHST3_MOUSE
AccessioniPrimary (citable) accession number: O88199
Secondary accession number(s): Q794I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.