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O88174

- MCP_MOUSE

UniProt

O88174 - MCP_MOUSE

Protein

Membrane cofactor protein

Gene

Cd46

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    May be involved in the fusion of the spermatozoa with the oocyte during fertilization.1 Publication

    GO - Biological processi

    1. negative regulation of complement activation Source: Ensembl
    2. proteolysis Source: Ensembl
    3. single fertilization Source: UniProtKB-KW

    Keywords - Biological processi

    Fertilization

    Enzyme and pathway databases

    ReactomeiREACT_198562. Regulation of Complement cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane cofactor protein
    Alternative name(s):
    CD_antigen: CD46
    Gene namesi
    Name:Cd46
    Synonyms:Mcp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1203290. Cd46.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. cell surface Source: Ensembl
    3. extracellular region Source: UniProtKB-SubCell
    4. Golgi apparatus Source: Ensembl
    5. inner acrosomal membrane Source: UniProtKB-SubCell
    6. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Mice have normal testis and fertile sperm.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4444Sequence AnalysisAdd
    BLAST
    Chaini45 – 365321Membrane cofactor proteinPRO_0000238971Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi109 ↔ 151PROSITE-ProRule annotation
    Disulfide bondi137 ↔ 168PROSITE-ProRule annotation
    Disulfide bondi173 ↔ 221PROSITE-ProRule annotation
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi202 ↔ 234PROSITE-ProRule annotation
    Glycosylationi205 – 2051O-linked (GalNAc...)Sequence Analysis
    Disulfide bondi239 ↔ 281PROSITE-ProRule annotation
    Disulfide bondi267 ↔ 294PROSITE-ProRule annotation
    Glycosylationi301 – 3011O-linked (GalNAc...)Sequence Analysis
    Glycosylationi304 – 3041O-linked (GalNAc...)Sequence Analysis
    Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi312 – 3121O-linked (GalNAc...)Sequence Analysis

    Post-translational modificationi

    May be O-glycosylated.By similarity
    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO88174.

    PTM databases

    PhosphoSiteiO88174.

    Expressioni

    Tissue specificityi

    Present only in testis (at protein level).3 Publications

    Developmental stagei

    Not expressed until 29 dpc. Expressed in parallel with synthesis of spermatids.1 Publication

    Gene expression databases

    ArrayExpressiO88174.
    BgeeiO88174.
    CleanExiMM_CD46.
    GenevestigatoriO88174.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000016637.

    Structurei

    3D structure databases

    ProteinModelPortaliO88174.
    SMRiO88174. Positions 45-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini45 – 329285ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini351 – 36515CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei330 – 35021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 10662Sushi 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini107 – 17064Sushi 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini171 – 23666Sushi 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini237 – 29660Sushi 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG151270.
    GeneTreeiENSGT00750000117418.
    HOGENOMiHOG000113502.
    HOVERGENiHBG006335.
    InParanoidiO88174.
    KOiK04007.
    OMAiTCIKGFK.
    OrthoDBiEOG7XPZ64.
    PhylomeDBiO88174.
    TreeFamiTF334137.

    Family and domain databases

    InterProiIPR017341. M_CF_CD46.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view]
    PfamiPF00084. Sushi. 4 hits.
    [Graphical view]
    PIRSFiPIRSF037971. TLX_CD46. 1 hit.
    SMARTiSM00032. CCP. 4 hits.
    [Graphical view]
    SUPFAMiSSF57535. SSF57535. 4 hits.
    PROSITEiPS50923. SUSHI. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: O88174-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTAAPLMPDS THPCRRRKSY TFFWCSLGVY AEALLFLLSH LSDACELPRP    50
    FEAMELKGTP KLFYAVGEKI EYKCKKGYLY LSPYLMIATC EPNHTWVPIS 100
    DAGCIKVQCT MLQDPSFGKV YYIDGSFSWG ARAKFTCMEG YYVVGMSVLH 150
    CVLKGDDEAY WNGYPPHCEK IYCLPPPKIK NGTHTLTDIN VFKYHEAVSY 200
    SCDPTPGPDK FSLVGTSMIF CAGHNTWSNS PPECKVVKCP NPVLQNGRLI 250
    SGAGEIFSYQ STVMFECLQG FYMEGSSMVI CSANNSWEPS IPKCLKGPRP 300
    THPTKPPVYN YTGYPSPREG IFSQELDAWI IALIVITSIV GVFILCLIVL 350
    RCFEHRKKTN VSAAR 365
    Length:365
    Mass (Da):40,881
    Last modified:November 1, 1998 - v1
    Checksum:i84A4CA8EE165C629
    GO
    Isoform 2 (identifier: O88174-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         297-310: GPRPTHPTKPPVYN → VTF
         311-365: Missing.

    Note: Lacks transmembrane domain, probably secreted.

    Show »
    Length:299
    Mass (Da):33,495
    Checksum:i57BA1655FA1F1449
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei297 – 31014GPRPT…PPVYN → VTF in isoform 2. 1 PublicationVSP_019038Add
    BLAST
    Alternative sequencei311 – 36555Missing in isoform 2. 1 PublicationVSP_019039Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001566 mRNA. Translation: BAA31859.1.
    AB010919 mRNA. Translation: BAA34810.1.
    AB022600 mRNA. Translation: BAA86056.1.
    AK006632 mRNA. Translation: BAB24682.1.
    CCDSiCCDS35828.1. [O88174-1]
    RefSeqiNP_034908.1. NM_010778.3. [O88174-1]
    XP_006497301.1. XM_006497238.1.
    UniGeneiMm.12884.

    Genome annotation databases

    EnsembliENSMUST00000159563; ENSMUSP00000123901; ENSMUSG00000016493.
    ENSMUST00000162650; ENSMUSP00000123931; ENSMUSG00000016493. [O88174-1]
    GeneIDi17221.
    KEGGimmu:17221.
    UCSCiuc007eet.1. mouse. [O88174-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001566 mRNA. Translation: BAA31859.1 .
    AB010919 mRNA. Translation: BAA34810.1 .
    AB022600 mRNA. Translation: BAA86056.1 .
    AK006632 mRNA. Translation: BAB24682.1 .
    CCDSi CCDS35828.1. [O88174-1 ]
    RefSeqi NP_034908.1. NM_010778.3. [O88174-1 ]
    XP_006497301.1. XM_006497238.1.
    UniGenei Mm.12884.

    3D structure databases

    ProteinModelPortali O88174.
    SMRi O88174. Positions 45-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000016637.

    PTM databases

    PhosphoSitei O88174.

    Proteomic databases

    PRIDEi O88174.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000159563 ; ENSMUSP00000123901 ; ENSMUSG00000016493 .
    ENSMUST00000162650 ; ENSMUSP00000123931 ; ENSMUSG00000016493 . [O88174-1 ]
    GeneIDi 17221.
    KEGGi mmu:17221.
    UCSCi uc007eet.1. mouse. [O88174-1 ]

    Organism-specific databases

    CTDi 4179.
    MGIi MGI:1203290. Cd46.

    Phylogenomic databases

    eggNOGi NOG151270.
    GeneTreei ENSGT00750000117418.
    HOGENOMi HOG000113502.
    HOVERGENi HBG006335.
    InParanoidi O88174.
    KOi K04007.
    OMAi TCIKGFK.
    OrthoDBi EOG7XPZ64.
    PhylomeDBi O88174.
    TreeFami TF334137.

    Enzyme and pathway databases

    Reactomei REACT_198562. Regulation of Complement cascade.

    Miscellaneous databases

    NextBioi 291626.
    PROi O88174.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88174.
    Bgeei O88174.
    CleanExi MM_CD46.
    Genevestigatori O88174.

    Family and domain databases

    InterProi IPR017341. M_CF_CD46.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view ]
    Pfami PF00084. Sushi. 4 hits.
    [Graphical view ]
    PIRSFi PIRSF037971. TLX_CD46. 1 hit.
    SMARTi SM00032. CCP. 4 hits.
    [Graphical view ]
    SUPFAMi SSF57535. SSF57535. 4 hits.
    PROSITEi PS50923. SUSHI. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a murine homologue of membrane cofactor protein (CD46): preferential expression in testicular germ cells."
      Tsujimura A., Shida K., Kitamura M., Nomura M., Takeda J., Tanaka H., Matsumoto M., Matsumiya K., Okuyama A., Nishimune Y., Okabe M., Seya T.
      Biochem. J. 330:163-168(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6.
      Tissue: Testis.
    2. "Molecular cloning of rat and mouse membrane cofactor protein (MCP, CD46): preferential expression in testis and close linkage between the mouse Mcp and Cr2 genes on distal chromosome 1."
      Miwa T., Nonaka M., Okada N., Wakana S., Shiroishi T., Okada H.
      Immunogenetics 48:363-371(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Testis.
    3. "Membrane and secretory forms of mouse membrane cofactor protein (CD46) generated from a single gene through alternative splicing."
      Nomura M., Tsujimura A., Shida K., Matsumoto M., Matsuda Y., Toyoshima K., Seya T.
      Immunogenetics 50:245-254(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Testis.
    5. "Disruption of mouse CD46 causes an accelerated spontaneous acrosome reaction in sperm."
      Inoue N., Ikawa M., Nakanishi T., Matsumoto M., Nomura M., Seya T., Okabe M.
      Mol. Cell. Biol. 23:2614-2622(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiMCP_MOUSE
    AccessioniPrimary (citable) accession number: O88174
    Secondary accession number(s): Q9R0R9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3