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O88174

- MCP_MOUSE

UniProt

O88174 - MCP_MOUSE

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Protein

Membrane cofactor protein

Gene

Cd46

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in the fusion of the spermatozoa with the oocyte during fertilization.1 Publication

GO - Biological processi

  1. interleukin-10 production Source: Ensembl
  2. negative regulation of complement activation Source: Ensembl
  3. negative regulation of gene expression Source: Ensembl
  4. positive regulation of gene expression Source: Ensembl
  5. positive regulation of interleukin-10 production Source: Ensembl
  6. positive regulation of memory T cell differentiation Source: Ensembl
  7. positive regulation of regulatory T cell differentiation Source: Ensembl
  8. positive regulation of T cell proliferation Source: Ensembl
  9. positive regulation of transforming growth factor beta production Source: Ensembl
  10. proteolysis Source: Ensembl
  11. regulation of Notch signaling pathway Source: Ensembl
  12. sequestering of extracellular ligand from receptor Source: Ensembl
  13. single fertilization Source: UniProtKB-KW
  14. T cell mediated immunity Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Fertilization

Enzyme and pathway databases

ReactomeiREACT_198562. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane cofactor protein
Alternative name(s):
CD_antigen: CD46
Gene namesi
Name:Cd46
Synonyms:Mcp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1203290. Cd46.

Subcellular locationi

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. cell surface Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. Golgi apparatus Source: Ensembl
  5. inner acrosomal membrane Source: Ensembl
  6. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice have normal testis and fertile sperm.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Sequence AnalysisAdd
BLAST
Chaini45 – 365321Membrane cofactor proteinPRO_0000238971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi109 ↔ 151PROSITE-ProRule annotation
Disulfide bondi137 ↔ 168PROSITE-ProRule annotation
Disulfide bondi173 ↔ 221PROSITE-ProRule annotation
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi202 ↔ 234PROSITE-ProRule annotation
Glycosylationi205 – 2051O-linked (GalNAc...)Sequence Analysis
Disulfide bondi239 ↔ 281PROSITE-ProRule annotation
Disulfide bondi267 ↔ 294PROSITE-ProRule annotation
Glycosylationi301 – 3011O-linked (GalNAc...)Sequence Analysis
Glycosylationi304 – 3041O-linked (GalNAc...)Sequence Analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi312 – 3121O-linked (GalNAc...)Sequence Analysis

Post-translational modificationi

May be O-glycosylated.By similarity
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO88174.

PTM databases

PhosphoSiteiO88174.

Expressioni

Tissue specificityi

Present only in testis (at protein level).3 Publications

Developmental stagei

Not expressed until 29 dpc. Expressed in parallel with synthesis of spermatids.1 Publication

Gene expression databases

BgeeiO88174.
CleanExiMM_CD46.
ExpressionAtlasiO88174. baseline and differential.
GenevestigatoriO88174.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000016637.

Structurei

3D structure databases

ProteinModelPortaliO88174.
SMRiO88174. Positions 45-296.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini45 – 329285ExtracellularSequence AnalysisAdd
BLAST
Topological domaini351 – 36515CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei330 – 35021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 10662Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini107 – 17064Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini171 – 23666Sushi 3PROSITE-ProRule annotationAdd
BLAST
Domaini237 – 29660Sushi 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG151270.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000113502.
HOVERGENiHBG006335.
InParanoidiO88174.
KOiK04007.
OMAiTCIKGFK.
OrthoDBiEOG7XPZ64.
PhylomeDBiO88174.
TreeFamiTF334137.

Family and domain databases

InterProiIPR017341. M_CF_CD46.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamiPF00084. Sushi. 4 hits.
[Graphical view]
PIRSFiPIRSF037971. TLX_CD46. 1 hit.
SMARTiSM00032. CCP. 4 hits.
[Graphical view]
SUPFAMiSSF57535. SSF57535. 4 hits.
PROSITEiPS50923. SUSHI. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: O88174) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAAPLMPDS THPCRRRKSY TFFWCSLGVY AEALLFLLSH LSDACELPRP
60 70 80 90 100
FEAMELKGTP KLFYAVGEKI EYKCKKGYLY LSPYLMIATC EPNHTWVPIS
110 120 130 140 150
DAGCIKVQCT MLQDPSFGKV YYIDGSFSWG ARAKFTCMEG YYVVGMSVLH
160 170 180 190 200
CVLKGDDEAY WNGYPPHCEK IYCLPPPKIK NGTHTLTDIN VFKYHEAVSY
210 220 230 240 250
SCDPTPGPDK FSLVGTSMIF CAGHNTWSNS PPECKVVKCP NPVLQNGRLI
260 270 280 290 300
SGAGEIFSYQ STVMFECLQG FYMEGSSMVI CSANNSWEPS IPKCLKGPRP
310 320 330 340 350
THPTKPPVYN YTGYPSPREG IFSQELDAWI IALIVITSIV GVFILCLIVL
360
RCFEHRKKTN VSAAR
Length:365
Mass (Da):40,881
Last modified:November 1, 1998 - v1
Checksum:i84A4CA8EE165C629
GO
Isoform 2 (identifier: O88174-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-310: GPRPTHPTKPPVYN → VTF
     311-365: Missing.

Note: Lacks transmembrane domain, probably secreted.

Show »
Length:299
Mass (Da):33,495
Checksum:i57BA1655FA1F1449
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei297 – 31014GPRPT…PPVYN → VTF in isoform 2. 1 PublicationVSP_019038Add
BLAST
Alternative sequencei311 – 36555Missing in isoform 2. 1 PublicationVSP_019039Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001566 mRNA. Translation: BAA31859.1.
AB010919 mRNA. Translation: BAA34810.1.
AB022600 mRNA. Translation: BAA86056.1.
AK006632 mRNA. Translation: BAB24682.1.
CCDSiCCDS35828.1. [O88174-1]
RefSeqiNP_034908.1. NM_010778.3. [O88174-1]
XP_006497301.1. XM_006497238.1.
UniGeneiMm.12884.

Genome annotation databases

EnsembliENSMUST00000159563; ENSMUSP00000123901; ENSMUSG00000016493.
ENSMUST00000162650; ENSMUSP00000123931; ENSMUSG00000016493. [O88174-1]
GeneIDi17221.
KEGGimmu:17221.
UCSCiuc007eet.1. mouse. [O88174-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001566 mRNA. Translation: BAA31859.1 .
AB010919 mRNA. Translation: BAA34810.1 .
AB022600 mRNA. Translation: BAA86056.1 .
AK006632 mRNA. Translation: BAB24682.1 .
CCDSi CCDS35828.1. [O88174-1 ]
RefSeqi NP_034908.1. NM_010778.3. [O88174-1 ]
XP_006497301.1. XM_006497238.1.
UniGenei Mm.12884.

3D structure databases

ProteinModelPortali O88174.
SMRi O88174. Positions 45-296.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000016637.

PTM databases

PhosphoSitei O88174.

Proteomic databases

PRIDEi O88174.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000159563 ; ENSMUSP00000123901 ; ENSMUSG00000016493 .
ENSMUST00000162650 ; ENSMUSP00000123931 ; ENSMUSG00000016493 . [O88174-1 ]
GeneIDi 17221.
KEGGi mmu:17221.
UCSCi uc007eet.1. mouse. [O88174-1 ]

Organism-specific databases

CTDi 4179.
MGIi MGI:1203290. Cd46.

Phylogenomic databases

eggNOGi NOG151270.
GeneTreei ENSGT00760000118803.
HOGENOMi HOG000113502.
HOVERGENi HBG006335.
InParanoidi O88174.
KOi K04007.
OMAi TCIKGFK.
OrthoDBi EOG7XPZ64.
PhylomeDBi O88174.
TreeFami TF334137.

Enzyme and pathway databases

Reactomei REACT_198562. Regulation of Complement cascade.

Miscellaneous databases

NextBioi 291626.
PROi O88174.
SOURCEi Search...

Gene expression databases

Bgeei O88174.
CleanExi MM_CD46.
ExpressionAtlasi O88174. baseline and differential.
Genevestigatori O88174.

Family and domain databases

InterProi IPR017341. M_CF_CD46.
IPR000436. Sushi_SCR_CCP.
[Graphical view ]
Pfami PF00084. Sushi. 4 hits.
[Graphical view ]
PIRSFi PIRSF037971. TLX_CD46. 1 hit.
SMARTi SM00032. CCP. 4 hits.
[Graphical view ]
SUPFAMi SSF57535. SSF57535. 4 hits.
PROSITEi PS50923. SUSHI. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a murine homologue of membrane cofactor protein (CD46): preferential expression in testicular germ cells."
    Tsujimura A., Shida K., Kitamura M., Nomura M., Takeda J., Tanaka H., Matsumoto M., Matsumiya K., Okuyama A., Nishimune Y., Okabe M., Seya T.
    Biochem. J. 330:163-168(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6.
    Tissue: Testis.
  2. "Molecular cloning of rat and mouse membrane cofactor protein (MCP, CD46): preferential expression in testis and close linkage between the mouse Mcp and Cr2 genes on distal chromosome 1."
    Miwa T., Nonaka M., Okada N., Wakana S., Shiroishi T., Okada H.
    Immunogenetics 48:363-371(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Testis.
  3. "Membrane and secretory forms of mouse membrane cofactor protein (CD46) generated from a single gene through alternative splicing."
    Nomura M., Tsujimura A., Shida K., Matsumoto M., Matsuda Y., Toyoshima K., Seya T.
    Immunogenetics 50:245-254(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "Disruption of mouse CD46 causes an accelerated spontaneous acrosome reaction in sperm."
    Inoue N., Ikawa M., Nakanishi T., Matsumoto M., Nomura M., Seya T., Okabe M.
    Mol. Cell. Biol. 23:2614-2622(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMCP_MOUSE
AccessioniPrimary (citable) accession number: O88174
Secondary accession number(s): Q9R0R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3