Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Membrane cofactor protein

Gene

Cd46

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the fusion of the spermatozoa with the oocyte during fertilization.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fertilization

Enzyme and pathway databases

ReactomeiR-MMU-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane cofactor protein
Alternative name(s):
CD_antigen: CD46
Gene namesi
Name:Cd46
Synonyms:Mcp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1203290. Cd46.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini45 – 329ExtracellularSequence analysisAdd BLAST285
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 365CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice have normal testis and fertile sperm.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 44Sequence analysisAdd BLAST44
ChainiPRO_000023897145 – 365Membrane cofactor proteinAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi109 ↔ 151PROSITE-ProRule annotation
Disulfide bondi137 ↔ 168PROSITE-ProRule annotation
Disulfide bondi173 ↔ 221PROSITE-ProRule annotation
Glycosylationi181N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi202 ↔ 234PROSITE-ProRule annotation
Glycosylationi205O-linked (GalNAc...)Sequence analysis1
Disulfide bondi239 ↔ 281PROSITE-ProRule annotation
Disulfide bondi267 ↔ 294PROSITE-ProRule annotation
Glycosylationi301O-linked (GalNAc...)Sequence analysis1
Glycosylationi304O-linked (GalNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi312O-linked (GalNAc...)Sequence analysis1

Post-translational modificationi

May be O-glycosylated.By similarity
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88174.
PRIDEiO88174.

PTM databases

PhosphoSitePlusiO88174.

Expressioni

Tissue specificityi

Present only in testis (at protein level).3 Publications

Developmental stagei

Not expressed until 29 dpc. Expressed in parallel with synthesis of spermatids.1 Publication

Gene expression databases

BgeeiENSMUSG00000016493.
CleanExiMM_CD46.
ExpressionAtlasiO88174. baseline and differential.
GenevisibleiO88174. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123931.

Structurei

3D structure databases

ProteinModelPortaliO88174.
SMRiO88174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 106Sushi 1PROSITE-ProRule annotationAdd BLAST62
Domaini107 – 170Sushi 2PROSITE-ProRule annotationAdd BLAST64
Domaini171 – 236Sushi 3PROSITE-ProRule annotationAdd BLAST66
Domaini237 – 296Sushi 4PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Contains 4 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY7G. Eukaryota.
ENOG4111CPD. LUCA.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000113502.
HOVERGENiHBG006335.
InParanoidiO88174.
KOiK04007.
OMAiFEAMELK.
OrthoDBiEOG091G0DWA.
PhylomeDBiO88174.
TreeFamiTF334137.

Family and domain databases

CDDicd00033. CCP. 4 hits.
InterProiIPR017341. CD46.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00084. Sushi. 4 hits.
[Graphical view]
PIRSFiPIRSF037971. TLX_CD46. 1 hit.
SMARTiSM00032. CCP. 4 hits.
[Graphical view]
SUPFAMiSSF57535. SSF57535. 4 hits.
PROSITEiPS50923. SUSHI. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O88174-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAAPLMPDS THPCRRRKSY TFFWCSLGVY AEALLFLLSH LSDACELPRP
60 70 80 90 100
FEAMELKGTP KLFYAVGEKI EYKCKKGYLY LSPYLMIATC EPNHTWVPIS
110 120 130 140 150
DAGCIKVQCT MLQDPSFGKV YYIDGSFSWG ARAKFTCMEG YYVVGMSVLH
160 170 180 190 200
CVLKGDDEAY WNGYPPHCEK IYCLPPPKIK NGTHTLTDIN VFKYHEAVSY
210 220 230 240 250
SCDPTPGPDK FSLVGTSMIF CAGHNTWSNS PPECKVVKCP NPVLQNGRLI
260 270 280 290 300
SGAGEIFSYQ STVMFECLQG FYMEGSSMVI CSANNSWEPS IPKCLKGPRP
310 320 330 340 350
THPTKPPVYN YTGYPSPREG IFSQELDAWI IALIVITSIV GVFILCLIVL
360
RCFEHRKKTN VSAAR
Length:365
Mass (Da):40,881
Last modified:November 1, 1998 - v1
Checksum:i84A4CA8EE165C629
GO
Isoform 2 (identifier: O88174-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-310: GPRPTHPTKPPVYN → VTF
     311-365: Missing.

Note: Lacks transmembrane domain, probably secreted.
Show »
Length:299
Mass (Da):33,495
Checksum:i57BA1655FA1F1449
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019038297 – 310GPRPT…PPVYN → VTF in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_019039311 – 365Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001566 mRNA. Translation: BAA31859.1.
AB010919 mRNA. Translation: BAA34810.1.
AB022600 mRNA. Translation: BAA86056.1.
AK006632 mRNA. Translation: BAB24682.1.
CCDSiCCDS35828.1. [O88174-1]
RefSeqiNP_034908.1. NM_010778.4. [O88174-1]
UniGeneiMm.12884.

Genome annotation databases

EnsembliENSMUST00000162650; ENSMUSP00000123931; ENSMUSG00000016493. [O88174-1]
GeneIDi17221.
KEGGimmu:17221.
UCSCiuc007eet.1. mouse. [O88174-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001566 mRNA. Translation: BAA31859.1.
AB010919 mRNA. Translation: BAA34810.1.
AB022600 mRNA. Translation: BAA86056.1.
AK006632 mRNA. Translation: BAB24682.1.
CCDSiCCDS35828.1. [O88174-1]
RefSeqiNP_034908.1. NM_010778.4. [O88174-1]
UniGeneiMm.12884.

3D structure databases

ProteinModelPortaliO88174.
SMRiO88174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123931.

PTM databases

PhosphoSitePlusiO88174.

Proteomic databases

PaxDbiO88174.
PRIDEiO88174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000162650; ENSMUSP00000123931; ENSMUSG00000016493. [O88174-1]
GeneIDi17221.
KEGGimmu:17221.
UCSCiuc007eet.1. mouse. [O88174-1]

Organism-specific databases

CTDi4179.
MGIiMGI:1203290. Cd46.

Phylogenomic databases

eggNOGiENOG410IY7G. Eukaryota.
ENOG4111CPD. LUCA.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000113502.
HOVERGENiHBG006335.
InParanoidiO88174.
KOiK04007.
OMAiFEAMELK.
OrthoDBiEOG091G0DWA.
PhylomeDBiO88174.
TreeFamiTF334137.

Enzyme and pathway databases

ReactomeiR-MMU-977606. Regulation of Complement cascade.

Miscellaneous databases

ChiTaRSiCd46. mouse.
PROiO88174.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016493.
CleanExiMM_CD46.
ExpressionAtlasiO88174. baseline and differential.
GenevisibleiO88174. MM.

Family and domain databases

CDDicd00033. CCP. 4 hits.
InterProiIPR017341. CD46.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00084. Sushi. 4 hits.
[Graphical view]
PIRSFiPIRSF037971. TLX_CD46. 1 hit.
SMARTiSM00032. CCP. 4 hits.
[Graphical view]
SUPFAMiSSF57535. SSF57535. 4 hits.
PROSITEiPS50923. SUSHI. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCP_MOUSE
AccessioniPrimary (citable) accession number: O88174
Secondary accession number(s): Q9R0R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.