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O88174 (MCP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Membrane cofactor protein
Alternative name(s):
CD_antigen=CD46
Gene names
Name:Cd46
Synonyms:Mcp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Ref.5

Subcellular location

Isoform 1: Cytoplasmic vesiclesecretory vesicleacrosome inner membrane; Single-pass type I membrane protein. Note: Inner acrosomal membrane of spermatozoa. Ref.5

Isoform 2: Secreted Probable Ref.5.

Tissue specificity

Present only in testis (at protein level). Ref.1 Ref.2 Ref.5

Developmental stage

Not expressed until 29 dpc. Expressed in parallel with synthesis of spermatids. Ref.1

Post-translational modification

May be O-glycosylated By similarity. Ref.5

N-glycosylated. Ref.5

Disruption phenotype

Mice have normal testis and fertile sperm. Ref.5

Sequence similarities

Contains 4 Sushi (CCP/SCR) domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O88174-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O88174-2)

The sequence of this isoform differs from the canonical sequence as follows:
     297-310: GPRPTHPTKPPVYN → VTF
     311-365: Missing.
Note: Lacks transmembrane domain, probably secreted.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4444 Potential
Chain45 – 365321Membrane cofactor protein
PRO_0000238971

Regions

Topological domain45 – 329285Extracellular Potential
Transmembrane330 – 35021Helical; Potential
Topological domain351 – 36515Cytoplasmic Potential
Domain45 – 10662Sushi 1
Domain107 – 17064Sushi 2
Domain171 – 23666Sushi 3
Domain237 – 29660Sushi 4

Amino acid modifications

Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation2051O-linked (GalNAc...) Potential
Glycosylation3011O-linked (GalNAc...) Potential
Glycosylation3041O-linked (GalNAc...) Potential
Glycosylation3101N-linked (GlcNAc...) Potential
Glycosylation3121O-linked (GalNAc...) Potential
Disulfide bond109 ↔ 151 By similarity
Disulfide bond137 ↔ 168 By similarity
Disulfide bond173 ↔ 221 By similarity
Disulfide bond202 ↔ 234 By similarity
Disulfide bond239 ↔ 281 By similarity
Disulfide bond267 ↔ 294 By similarity

Natural variations

Alternative sequence297 – 31014GPRPT…PPVYN → VTF in isoform 2.
VSP_019038
Alternative sequence311 – 36555Missing in isoform 2.
VSP_019039

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 84A4CA8EE165C629

FASTA36540,881
        10         20         30         40         50         60 
MTAAPLMPDS THPCRRRKSY TFFWCSLGVY AEALLFLLSH LSDACELPRP FEAMELKGTP 

        70         80         90        100        110        120 
KLFYAVGEKI EYKCKKGYLY LSPYLMIATC EPNHTWVPIS DAGCIKVQCT MLQDPSFGKV 

       130        140        150        160        170        180 
YYIDGSFSWG ARAKFTCMEG YYVVGMSVLH CVLKGDDEAY WNGYPPHCEK IYCLPPPKIK 

       190        200        210        220        230        240 
NGTHTLTDIN VFKYHEAVSY SCDPTPGPDK FSLVGTSMIF CAGHNTWSNS PPECKVVKCP 

       250        260        270        280        290        300 
NPVLQNGRLI SGAGEIFSYQ STVMFECLQG FYMEGSSMVI CSANNSWEPS IPKCLKGPRP 

       310        320        330        340        350        360 
THPTKPPVYN YTGYPSPREG IFSQELDAWI IALIVITSIV GVFILCLIVL RCFEHRKKTN 


VSAAR 

« Hide

Isoform 2 [UniParc].

Checksum: 57BA1655FA1F1449
Show »

FASTA29933,495

References

« Hide 'large scale' references
[1]"Molecular cloning of a murine homologue of membrane cofactor protein (CD46): preferential expression in testicular germ cells."
Tsujimura A., Shida K., Kitamura M., Nomura M., Takeda J., Tanaka H., Matsumoto M., Matsumiya K., Okuyama A., Nishimune Y., Okabe M., Seya T.
Biochem. J. 330:163-168(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6.
Tissue: Testis.
[2]"Molecular cloning of rat and mouse membrane cofactor protein (MCP, CD46): preferential expression in testis and close linkage between the mouse Mcp and Cr2 genes on distal chromosome 1."
Miwa T., Nonaka M., Okada N., Wakana S., Shiroishi T., Okada H.
Immunogenetics 48:363-371(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Testis.
[3]"Membrane and secretory forms of mouse membrane cofactor protein (CD46) generated from a single gene through alternative splicing."
Nomura M., Tsujimura A., Shida K., Matsumoto M., Matsuda Y., Toyoshima K., Seya T.
Immunogenetics 50:245-254(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Testis.
[5]"Disruption of mouse CD46 causes an accelerated spontaneous acrosome reaction in sperm."
Inoue N., Ikawa M., Nakanishi T., Matsumoto M., Nomura M., Seya T., Okabe M.
Mol. Cell. Biol. 23:2614-2622(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB001566 mRNA. Translation: BAA31859.1.
AB010919 mRNA. Translation: BAA34810.1.
AB022600 mRNA. Translation: BAA86056.1.
AK006632 mRNA. Translation: BAB24682.1.
CCDSCCDS35828.1. [O88174-1]
RefSeqNP_034908.1. NM_010778.3. [O88174-1]
XP_006497301.1. XM_006497238.1.
UniGeneMm.12884.

3D structure databases

ProteinModelPortalO88174.
SMRO88174. Positions 45-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000016637.

PTM databases

PhosphoSiteO88174.

Proteomic databases

PRIDEO88174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000159563; ENSMUSP00000123901; ENSMUSG00000016493.
ENSMUST00000162650; ENSMUSP00000123931; ENSMUSG00000016493. [O88174-1]
GeneID17221.
KEGGmmu:17221.
UCSCuc007eet.1. mouse. [O88174-1]

Organism-specific databases

CTD4179.
MGIMGI:1203290. Cd46.

Phylogenomic databases

eggNOGNOG151270.
GeneTreeENSGT00750000117418.
HOGENOMHOG000113502.
HOVERGENHBG006335.
InParanoidO88174.
KOK04007.
OMATCIKGFK.
OrthoDBEOG7XPZ64.
PhylomeDBO88174.
TreeFamTF334137.

Gene expression databases

ArrayExpressO88174.
BgeeO88174.
CleanExMM_CD46.
GenevestigatorO88174.

Family and domain databases

InterProIPR017341. M_CF_CD46.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamPF00084. Sushi. 4 hits.
[Graphical view]
PIRSFPIRSF037971. TLX_CD46. 1 hit.
SMARTSM00032. CCP. 4 hits.
[Graphical view]
SUPFAMSSF57535. SSF57535. 4 hits.
PROSITEPS50923. SUSHI. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio291626.
PROO88174.
SOURCESearch...

Entry information

Entry nameMCP_MOUSE
AccessionPrimary (citable) accession number: O88174
Secondary accession number(s): Q9R0R9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot