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Protein

Hemoglobin-binding protease hbp autotransporter

Gene

hbp

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with hemoglobin, degrades it and subsequently binds the released heme. Could make heme accessible not only for E.coli, but also for B.fragilis during mixed intra-abdominal infections. Has a role in abscess formation.2 Publications

Enzyme regulationi

Protease activity is inhibited by 3,4-dichloroisocoumarin.

pH dependencei

Optimum pH is 6.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125Charge relay system1
Active sitei153Charge relay system1
Active sitei259Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Hemoglobin-binding protease hbp autotransporter (EC:3.4.21.-)
Cleaved into the following 2 chains:
Gene namesi
Name:hbp
Encoded oniPlasmid IncFI ColV3-K300 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

Hemoglobin-binding protease hbp translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 521 PublicationAdd BLAST52
ChainiPRO_000038759753 – 1377Hemoglobin-binding protease hbp autotransporterAdd BLAST1325
ChainiPRO_000004198253 – 1100Hemoglobin-binding protease hbpAdd BLAST1048
ChainiPRO_00000419831101 – 1377Hemoglobin-binding protease hbp translocatorAdd BLAST277

Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1100 – 1101Cleavage2

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiO88093.

Structurei

Secondary structure

11377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi54 – 59Combined sources6
Helixi62 – 68Combined sources7
Helixi72 – 74Combined sources3
Beta strandi80 – 84Combined sources5
Beta strandi90 – 94Combined sources5
Turni107 – 109Combined sources3
Beta strandi113 – 116Combined sources4
Beta strandi119 – 122Combined sources4
Beta strandi141 – 145Combined sources5
Beta strandi150 – 153Combined sources4
Beta strandi158 – 161Combined sources4
Helixi180 – 182Combined sources3
Turni184 – 186Combined sources3
Beta strandi190 – 194Combined sources5
Beta strandi198 – 201Combined sources4
Beta strandi207 – 211Combined sources5
Beta strandi218 – 222Combined sources5
Beta strandi226 – 228Combined sources3
Turni229 – 232Combined sources4
Beta strandi233 – 236Combined sources4
Helixi244 – 247Combined sources4
Beta strandi249 – 251Combined sources3
Beta strandi262 – 267Combined sources6
Turni268 – 271Combined sources4
Beta strandi272 – 284Combined sources13
Beta strandi290 – 294Combined sources5
Helixi297 – 305Combined sources9
Helixi316 – 318Combined sources3
Beta strandi322 – 326Combined sources5
Turni328 – 330Combined sources3
Beta strandi332 – 337Combined sources6
Beta strandi340 – 345Combined sources6
Helixi352 – 354Combined sources3
Beta strandi357 – 362Combined sources6
Beta strandi365 – 371Combined sources7
Beta strandi379 – 384Combined sources6
Beta strandi386 – 392Combined sources7
Beta strandi396 – 403Combined sources8
Beta strandi408 – 411Combined sources4
Beta strandi420 – 431Combined sources12
Beta strandi434 – 436Combined sources3
Beta strandi439 – 443Combined sources5
Beta strandi445 – 450Combined sources6
Beta strandi466 – 468Combined sources3
Beta strandi474 – 479Combined sources6
Helixi485 – 487Combined sources3
Beta strandi488 – 490Combined sources3
Beta strandi495 – 498Combined sources4
Beta strandi504 – 507Combined sources4
Beta strandi516 – 520Combined sources5
Beta strandi522 – 524Combined sources3
Beta strandi526 – 530Combined sources5
Helixi536 – 538Combined sources3
Beta strandi555 – 560Combined sources6
Turni561 – 564Combined sources4
Beta strandi565 – 571Combined sources7
Beta strandi573 – 575Combined sources3
Beta strandi581 – 583Combined sources3
Beta strandi587 – 591Combined sources5
Helixi596 – 607Combined sources12
Beta strandi612 – 614Combined sources3
Beta strandi616 – 627Combined sources12
Beta strandi635 – 638Combined sources4
Beta strandi640 – 657Combined sources18
Helixi669 – 677Combined sources9
Beta strandi685 – 687Combined sources3
Beta strandi698 – 709Combined sources12
Beta strandi711 – 714Combined sources4
Beta strandi718 – 728Combined sources11
Beta strandi730 – 734Combined sources5
Beta strandi736 – 741Combined sources6
Turni742 – 745Combined sources4
Beta strandi746 – 748Combined sources3
Beta strandi752 – 756Combined sources5
Helixi762 – 764Combined sources3
Beta strandi767 – 777Combined sources11
Beta strandi779 – 782Combined sources4
Beta strandi784 – 794Combined sources11
Beta strandi796 – 799Combined sources4
Beta strandi802 – 806Combined sources5
Beta strandi808 – 813Combined sources6
Beta strandi815 – 818Combined sources4
Beta strandi823 – 826Combined sources4
Beta strandi830 – 847Combined sources18
Beta strandi854 – 856Combined sources3
Beta strandi859 – 870Combined sources12
Beta strandi874 – 877Combined sources4
Beta strandi879 – 892Combined sources14
Beta strandi894 – 897Combined sources4
Helixi909 – 917Combined sources9
Turni918 – 920Combined sources3
Beta strandi922 – 927Combined sources6
Beta strandi929 – 945Combined sources17
Beta strandi949 – 958Combined sources10
Beta strandi960 – 963Combined sources4
Beta strandi967 – 969Combined sources3
Beta strandi972 – 982Combined sources11
Beta strandi984 – 988Combined sources5
Beta strandi996 – 1006Combined sources11
Beta strandi1008 – 1013Combined sources6
Beta strandi1020 – 1022Combined sources3
Beta strandi1026 – 1032Combined sources7
Helixi1037 – 1039Combined sources3
Beta strandi1040 – 1042Combined sources3
Beta strandi1048 – 1051Combined sources4
Beta strandi1053 – 1059Combined sources7
Beta strandi1064 – 1073Combined sources10
Helixi1075 – 1097Combined sources23
Helixi1102 – 1106Combined sources5
Beta strandi1115 – 1128Combined sources14
Beta strandi1134 – 1150Combined sources17
Beta strandi1153 – 1170Combined sources18
Beta strandi1173 – 1190Combined sources18
Beta strandi1193 – 1211Combined sources19
Beta strandi1217 – 1234Combined sources18
Beta strandi1236 – 1257Combined sources22
Beta strandi1279 – 1292Combined sources14
Beta strandi1294 – 1311Combined sources18
Beta strandi1331 – 1345Combined sources15
Turni1346 – 1348Combined sources3
Beta strandi1349 – 1360Combined sources12
Beta strandi1362 – 1377Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXRX-ray2.20A53-1100[»]
3AEHX-ray2.00A/B1075-1377[»]
3AK5X-ray2.20A/B/C/D53-533[»]
A/B/C/D608-1100[»]
ProteinModelPortaliO88093.
SMRiO88093.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88093.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 302Peptidase S6PROSITE-ProRule annotationAdd BLAST250
Domaini1111 – 1377AutotransporterPROSITE-ProRule annotationAdd BLAST267

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage.

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Family and domain databases

Gene3Di2.160.20.20. 3 hits.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR009003. Peptidase_S1_PA.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF50494. SSF50494. 2 hits.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIYSLRYS AVARGFIAVS EFARKCVHKS VRRLCFPVLL LIPVLFSAGS
60 70 80 90 100
LAGTVNNELG YQLFRDFAEN KGMFRPGATN IAIYNKQGEF VGTLDKAAMP
110 120 130 140 150
DFSAVDSEIG VATLINPQYI ASVKHNGGYT NVSFGDGENR YNIVDRNNAP
160 170 180 190 200
SLDFHAPRLD KLVTEVAPTA VTAQGAVAGA YLDKERYPVF YRLGSGTQYI
210 220 230 240 250
KDSNGQLTKM GGAYSWLTGG TVGSLSSYQN GEMISTSSGL VFDYKLNGAM
260 270 280 290 300
PIYGEAGDSG SPLFAFDTVQ NKWVLVGVLT AGNGAGGRGN NWAVIPLDFI
310 320 330 340 350
GQKFNEDNDA PVTFRTSEGG ALEWSFNSST GAGALTQGTT TYAMHGQQGN
360 370 380 390 400
DLNAGKNLIF QGQNGQINLK DSVSQGAGSL TFRDNYTVTT SNGSTWTGAG
410 420 430 440 450
IVVDNGVSVN WQVNGVKGDN LHKIGEGTLT VQGTGINEGG LKVGDGKVVL
460 470 480 490 500
NQQADNKGQV QAFSSVNIAS GRPTVVLTDE RQVNPDTVSW GYRGGTLDVN
510 520 530 540 550
GNSLTFHQLK AADYGAVLAN NVDKRATITL DYALRADKVA LNGWSESGKG
560 570 580 590 600
TAGNLYKYNN PYTNTTDYFI LKQSTYGYFP TDQSSNATWE FVGHSQGDAQ
610 620 630 640 650
KLVADRFNTA GYLFHGQLKG NLNVDNRLPE GVTGALVMDG AADISGTFTQ
660 670 680 690 700
ENGRLTLQGH PVIHAYNTQS VADKLAASGD HSVLTQPTSF SQEDWENRSF
710 720 730 740 750
TFDRLSLKNT DFGLGRNATL NTTIQADNSS VTLGDSRVFI DKNDGQGTAF
760 770 780 790 800
TLEEGTSVAT KDADKSVFNG TVNLDNQSVL NINDIFNGGI QANNSTVNIS
810 820 830 840 850
SDSAVLGNST LTSTALNLNK GANALASQSF VSDGPVNISD ATLSLNSRPD
860 870 880 890 900
EVSHTLLPVY DYAGSWNLKG DDARLNVGPY SMLSGNINVQ DKGTVTLGGE
910 920 930 940 950
GELSPDLTLQ NQMLYSLFNG YRNIWSGSLN APDATVSMTD TQWSMNGNST
960 970 980 990 1000
AGNMKLNRTI VGFNGGTSPF TTLTTDNLDA VQSAFVMRTD LNKADKLVIN
1010 1020 1030 1040 1050
KSATGHDNSI WVNFLKKPSN KDTLDIPLVS APEATADNLF RASTRVVGFS
1060 1070 1080 1090 1100
DVTPILSVRK EDGKKEWVLD GYQVARNDGQ GKAAATFMHI SYNNFITEVN
1110 1120 1130 1140 1150
NLNKRMGDLR DINGEAGTWV RLLNGSGSAD GGFTDHYTLL QMGADRKHEL
1160 1170 1180 1190 1200
GSMDLFTGVM ATYTDTDASA DLYSGKTKSW GGGFYASGLF RSGAYFDVIA
1210 1220 1230 1240 1250
KYIHNENKYD LNFAGAGKQN FRSHSLYAGA EVGYRYHLTD TTFVEPQAEL
1260 1270 1280 1290 1300
VWGRLQGQTF NWNDSGMDVS MRRNSVNPLV GRTGVVSGKT FSGKDWSLTA
1310 1320 1330 1340 1350
RAGLHYEFDL TDSADVHLKD AAGEHQINGR KDSRMLYGVG LNARFGDNTR
1360 1370
LGLEVERSAF GKYNTDDAIN ANIRYSF
Length:1,377
Mass (Da):148,257
Last modified:November 1, 1998 - v1
Checksum:iBB16D898EDAC0416
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223631 Genomic DNA. Translation: CAA11507.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223631 Genomic DNA. Translation: CAA11507.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXRX-ray2.20A53-1100[»]
3AEHX-ray2.00A/B1075-1377[»]
3AK5X-ray2.20A/B/C/D53-533[»]
A/B/C/D608-1100[»]
ProteinModelPortaliO88093.
SMRiO88093.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO88093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO88093.

Family and domain databases

Gene3Di2.160.20.20. 3 hits.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR009003. Peptidase_S1_PA.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF50494. SSF50494. 2 hits.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHBP_ECOLX
AccessioniPrimary (citable) accession number: O88093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.