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Protein

Ktr system potassium uptake protein A

Gene

ktrA

Organism
Vibrio alginolyticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Na+-dependent high affinity K+ uptake system KtrAB. KtrA is the regulatory subunit and plays an important role in the substrate specificity and transport mechanism of the system. Binds ATP but lacks ATPase activity.3 Publications

Enzyme regulationi

Requires both ATP and a high membrane potential for activity. Binding of ATP causes a conformational change in KtrA, which promotes formation of the KtrAB complex. Can also bind, with lower affinity, other nucleotides such as NADH or NAD+, but only ATP can induce a conformational change.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATPBy similarity
Binding sitei124 – 1241ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 373ATPBy similarity
Nucleotide bindingi55 – 562ATPBy similarity
Nucleotide bindingi77 – 793ATPBy similarity
Nucleotide bindingi102 – 1043ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Potassium

Protein family/group databases

TCDBi2.A.38.4.2. the k(+) transporter (trk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ktr system potassium uptake protein A
Short name:
K(+)-uptake protein KtrA
Gene namesi
Name:ktrA
OrganismiVibrio alginolyticus
Taxonomic identifieri663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ktr system potassium uptake protein APRO_0000430032Add
BLAST

Interactioni

Subunit structurei

The uptake system is composed of KtrA and KtrB.2 Publications

Protein-protein interaction databases

STRINGi674977.VMC_33760.

Structurei

3D structure databases

ProteinModelPortaliO87952.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 129123RCK N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini138 – 22083RCK C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the KtrA potassium transport family.Curated
Contains 1 RCK C-terminal domain.PROSITE-ProRule annotation
Contains 1 RCK N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107ZYR. Bacteria.
COG0569. LUCA.

Family and domain databases

Gene3Di3.30.70.1450. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR006037. RCK_C.
IPR003148. RCK_N.
[Graphical view]
PfamiPF02080. TrkA_C. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
SUPFAMiSSF116726. SSF116726. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS51202. RCK_C. 1 hit.
PS51201. RCK_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTGDKQFAV IGLGRFGLAV CKELQDSGSQ VLAVDINEDR VKEAAGFVSQ
60 70 80 90 100
AIVANCTHEE TVAELKLDDY DMVMIAIGAD VNASILATLI AKEAGVKSVW
110 120 130 140 150
VKANDRFQAR VLQKIGADHI IMPERDMGIR VARKMLDKRV LEFHPLGSGL
160 170 180 190 200
AMTEFVVGSR LMGKTLSDLA LCKVEGVQVL GYKRGPEIIK APDMSTTLEI
210 220
GDLIIVVGPQ DKLANKLKSL
Length:220
Mass (Da):23,806
Last modified:November 1, 1998 - v1
Checksum:i239DEA9CD046AD36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89592 Genomic DNA. Translation: BAA31234.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89592 Genomic DNA. Translation: BAA31234.1.

3D structure databases

ProteinModelPortaliO87952.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi674977.VMC_33760.

Protein family/group databases

TCDBi2.A.38.4.2. the k(+) transporter (trk) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107ZYR. Bacteria.
COG0569. LUCA.

Family and domain databases

Gene3Di3.30.70.1450. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR006037. RCK_C.
IPR003148. RCK_N.
[Graphical view]
PfamiPF02080. TrkA_C. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
SUPFAMiSSF116726. SSF116726. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS51202. RCK_C. 1 hit.
PS51201. RCK_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "KtrAB, a new type of bacterial K(+)-uptake system from Vibrio alginolyticus."
    Nakamura T., Yuda R., Unemoto T., Bakker E.P.
    J. Bacteriol. 180:3491-3494(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, GENE NAME.
  2. "All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K+ uptake by the KtrAB system from Vibrio alginolyticus."
    Tholema N., Vor der Brueggen M., Maeser P., Nakamura T., Schroeder J.I., Kobayashi H., Uozumi N., Bakker E.P.
    J. Biol. Chem. 280:41146-41154(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  3. "ATP binding to the KTN/RCK subunit KtrA from the K+ -uptake system KtrAB of Vibrio alginolyticus: its role in the formation of the KtrAB complex and its requirement in vivo."
    Kroening N., Willenborg M., Tholema N., Haenelt I., Schmid R., Bakker E.P.
    J. Biol. Chem. 282:14018-14027(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ATP-BINDING, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKTRA_VIBAL
AccessioniPrimary (citable) accession number: O87952
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: November 1, 1998
Last modified: December 9, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.