ID   BCRD_THAAR              Reviewed;         282 AA.
AC   O87877;
DT   23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=Benzoyl-CoA reductase subunit D;
DE            EC=1.3.99.15;
DE   AltName: Full=3-hydroxybenzoyl-CoA reductase subunit delta;
DE            EC=1.3.99.n1;
GN   Name=bcrD;
OS   Thauera aromatica.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Thauera.
OX   NCBI_TaxID=59405;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-14, AND
RP   SUBUNIT.
RC   STRAIN=DSM 6984 / K172;
RX   MEDLINE=98417440; PubMed=9746358;
RX   DOI=10.1046/j.1432-1327.1998.2560148.x;
RA   Breese K., Boll M., Alt-Moerbe J., Schaegger H., Fuchs G.;
RT   "Genes coding for the benzoyl-CoA pathway of anaerobic aromatic
RT   metabolism in the bacterium Thauera aromatica.";
RL   Eur. J. Biochem. 256:148-154(1998).
RN   [2]
RP   CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND COFACTOR.
RC   STRAIN=DSM 6984 / K172;
RX   PubMed=8575453; DOI=10.1111/j.1432-1033.1995.921_a.x;
RA   Boll M., Fuchs G.;
RT   "Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of
RT   anaerobic aromatic metabolism. ATP dependence of the reaction,
RT   purification and some properties of the enzyme from Thauera aromatica
RT   strain K172.";
RL   Eur. J. Biochem. 234:921-933(1995).
RN   [3]
RP   CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=DSM 6984 / K172;
RX   MEDLINE=21142524; PubMed=11208796; DOI=10.1128/JB.183.3.968-979.2001;
RA   Laempe D., Jahn M., Breese K., Schaegger H., Fuchs G.;
RT   "Anaerobic metabolism of 3-hydroxybenzoate by the denitrifying
RT   bacterium Thauera aromatica.";
RL   J. Bacteriol. 183:968-979(2001).
CC   -!- FUNCTION: Catalyzes the anaerobic reduction of benzoyl-CoA and 3-
CC       hydroxybenzoyl-CoA to form cyclohexa-1,5-diene-1-carbonyl-CoA and
CC       3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA, respectively. The
CC       enzyme also reduces other benzoyl-CoA analogs with small
CC       substituents at the aromatic ring.
CC   -!- CATALYTIC ACTIVITY: Benzoyl-CoA + reduced acceptor + 2 ATP =
CC       cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + 2 ADP + 2
CC       phosphate.
CC   -!- CATALYTIC ACTIVITY: 3-hydroxybenzoyl-CoA + reduced acceptor + 2
CC       ATP = 3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + 2
CC       ADP + 2 phosphate.
CC   -!- COFACTOR: Iron-sulfur. May be a 4Fe-4S cluster.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=15 uM for benzoyl-CoA;
CC         KM=20 uM for 3-hydroxybenzoyl-CoA;
CC         KM=600 uM for ATP;
CC       pH dependence:
CC         Optimum pH is 7.2-7.5;
CC   -!- SUBUNIT: Heterotetramer composed of A, B, C, and D subunits.
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DR   EMBL; AJ224959; CAA12250.1; -; Genomic_DNA.
DR   HSSP; P11568; 1HUX.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018522; F:benzoyl-CoA reductase activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002731; ATPase_BadF.
DR   InterPro; IPR011956; Benzoyl_CoA_Rdtase_D.
DR   InterPro; IPR008275; CoA_E_activase.
DR   Pfam; PF01869; BcrAD_BadFG; 1.
DR   ProDom; PD006344; CoA_E_activase; 1.
DR   TIGRFAMs; TIGR02261; benz_CoA_red_D; 1.
DR   TIGRFAMs; TIGR00241; CoA_E_activ; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Aromatic hydrocarbons catabolism; ATP-binding;
KW   Direct protein sequencing; Iron; Iron-sulfur; Metal-binding;
KW   Nucleotide-binding; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    282       Benzoyl-CoA reductase subunit D.
FT                                /FTId=PRO_0000350733.
FT   METAL       130    130       Iron-sulfur (4Fe-4S); shared with bcrA
FT                                (Potential).
FT   METAL       169    169       Iron-sulfur (4Fe-4S); shared with bcrA
FT                                (Potential).
SQ   SEQUENCE   282 AA;  30157 MW;  248D3D6305C90514 CRC64;
     MTITAGIDIG TGAVKTVLFR VEGDKTEWLA KRNDRIRQRD PFKLAEEAYN GLLEEAGLKA
     SDVDYVATTG EGESLAFHTG HFYSMTTHAR GAVYLNPEAR AVLDIGALHG RAIRNDERGK
     VETYKMTSQC ASGSGQFLEN IARYLGIAQD EIGSLSTQAD NPEVVSSICA VLAETDVINM
     VSRGISAPNI LKGIHISMAG RLAKLLKSVG ARDGVVLCTG GLALDEGLLK TLNESIQEQK
     MAVVAYNHPD SPYAGAIGAA LWGAFRHEKL ARLGQQQVAE AA
//