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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi407Magnesium 1By similarity1
Metal bindingi414Magnesium 1By similarity1
Metal bindingi414Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:R02904
ORF Names:SMc03173
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001526721 – 498Lysine--tRNA ligaseAdd BLAST498

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi266834.SMc03173.

Structurei

3D structure databases

ProteinModelPortaliO87821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRK. Bacteria.
COG1190. LUCA.
HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKTQAAGL SSDATEVRSQ KLDLLRQQIG DVYPAHFHRT LTNAELAEKY
60 70 80 90 100
AGLEPDTESG ETVTVAGRVF SSRNSGMFMD LHDASGKIQI FSHKDTAPEE
110 120 130 140 150
ARALLPMIDL GDIIGVTGEV RRTKRGELTV NAKEITMLCK SLLPMPEKYH
160 170 180 190 200
GLADIETRYR KRYLDIMVNE ESKLRFQQRS RIVSSLRRFL EDEGFMEVET
210 220 230 240 250
PMLQPIYGGA TAEPFKTHHN TLKLDMYLRI APELYLKRIL VSGLTDKVFE
260 270 280 290 300
INRNFRNEGV STRHNPEFTM MECYWAYADY EDVMGLVERM FETLALAVHG
310 320 330 340 350
KTEFEFGDKQ LSFKGPFPRV SMPAAVKDAT GIDFLALKSD EEARQAARDA
360 370 380 390 400
GVEIEKDATW GEVLAFLFEE KVEATLIQPA HVIHFPKDIS PFAKEVPGEP
410 420 430 440 450
RLVERFETYC NGWEIGNAFS ELNDPVEQRA RMVEQMEQAH ARGEKEKTLD
460 470 480 490
EDFLDAMDQG MPPAGGLGIG VDRLIMLLTN SPSIRDIILF PARRQKAD
Length:498
Mass (Da):56,336
Last modified:November 2, 2001 - v2
Checksum:iFAF7D7052961C24A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232P → A in AAC35210 (PubMed:10225420).Curated1
Sequence conflicti237 – 238KR → NA in AAC35210 (PubMed:10225420).Curated2
Sequence conflicti326 – 336VKDATGIDFLA → SRTRPESISLP in AAC35210 (PubMed:10225420).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27221 Genomic DNA. Translation: AAC35210.1.
AL591688 Genomic DNA. Translation: CAC47483.1.
RefSeqiNP_387010.1. NC_003047.1.
WP_003528855.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC47483; CAC47483; SMc03173.
GeneIDi1234581.
KEGGisme:SMc03173.
PATRICi23635392. VBISinMel96828_4425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27221 Genomic DNA. Translation: AAC35210.1.
AL591688 Genomic DNA. Translation: CAC47483.1.
RefSeqiNP_387010.1. NC_003047.1.
WP_003528855.1. NC_003047.1.

3D structure databases

ProteinModelPortaliO87821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc03173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC47483; CAC47483; SMc03173.
GeneIDi1234581.
KEGGisme:SMc03173.
PATRICi23635392. VBISinMel96828_4425.

Phylogenomic databases

eggNOGiENOG4105CRK. Bacteria.
COG1190. LUCA.
HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYK_RHIME
AccessioniPrimary (citable) accession number: O87821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.