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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi407Magnesium 1By similarity1
Metal bindingi414Magnesium 1By similarity1
Metal bindingi414Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:R02904
ORF Names:SMc03173
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001526721 – 498Lysine--tRNA ligaseAdd BLAST498

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi266834.SMc03173

Structurei

3D structure databases

ProteinModelPortaliO87821
SMRiO87821
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRK Bacteria
COG1190 LUCA
HOGENOMiHOG000236578
KOiK04567
OMAiMMNIIPG

Family and domain databases

CDDicd00775 LysRS_core, 1 hit
HAMAPiMF_00252 Lys_tRNA_synth_class2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR002313 Lys-tRNA-ligase_II
IPR018149 Lys-tRNA-synth_II_C
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR00982 TRNASYNTHLYS
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00499 lysS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

O87821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKTQAAGL SSDATEVRSQ KLDLLRQQIG DVYPAHFHRT LTNAELAEKY
60 70 80 90 100
AGLEPDTESG ETVTVAGRVF SSRNSGMFMD LHDASGKIQI FSHKDTAPEE
110 120 130 140 150
ARALLPMIDL GDIIGVTGEV RRTKRGELTV NAKEITMLCK SLLPMPEKYH
160 170 180 190 200
GLADIETRYR KRYLDIMVNE ESKLRFQQRS RIVSSLRRFL EDEGFMEVET
210 220 230 240 250
PMLQPIYGGA TAEPFKTHHN TLKLDMYLRI APELYLKRIL VSGLTDKVFE
260 270 280 290 300
INRNFRNEGV STRHNPEFTM MECYWAYADY EDVMGLVERM FETLALAVHG
310 320 330 340 350
KTEFEFGDKQ LSFKGPFPRV SMPAAVKDAT GIDFLALKSD EEARQAARDA
360 370 380 390 400
GVEIEKDATW GEVLAFLFEE KVEATLIQPA HVIHFPKDIS PFAKEVPGEP
410 420 430 440 450
RLVERFETYC NGWEIGNAFS ELNDPVEQRA RMVEQMEQAH ARGEKEKTLD
460 470 480 490
EDFLDAMDQG MPPAGGLGIG VDRLIMLLTN SPSIRDIILF PARRQKAD
Length:498
Mass (Da):56,336
Last modified:November 2, 2001 - v2
Checksum:iFAF7D7052961C24A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232P → A in AAC35210 (PubMed:10225420).Curated1
Sequence conflicti237 – 238KR → NA in AAC35210 (PubMed:10225420).Curated2
Sequence conflicti326 – 336VKDATGIDFLA → SRTRPESISLP in AAC35210 (PubMed:10225420).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27221 Genomic DNA Translation: AAC35210.1
AL591688 Genomic DNA Translation: CAC47483.1
RefSeqiNP_387010.1, NC_003047.1
WP_003528855.1, NC_003047.1

Genome annotation databases

EnsemblBacteriaiCAC47483; CAC47483; SMc03173
GeneIDi1234581
KEGGisme:SMc03173
PATRICifig|266834.11.peg.4425

Similar proteinsi

Entry informationi

Entry nameiSYK_RHIME
AccessioniPrimary (citable) accession number: O87821
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: March 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health