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Protein

RNA polymerase sigma factor RpoD

Gene

rpoD

Organism
Pseudomonas tolaasii
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.SAAS annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi576 – 595H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Sigma factorUniRule annotationSAAS annotation
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoDUniRule annotation
Alternative name(s):
Sigma-70UniRule annotation
Gene namesi
Name:rpoDUniRule annotationImported
OrganismiPseudomonas tolaasiiImported
Taxonomic identifieri29442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliO87812
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini406 – 419RNA_pol_sigma70InterPro annotationAdd BLAST14
Domaini575 – 601RNA_pol_sigma70InterPro annotationAdd BLAST27

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni382 – 452Sigma-70 factor domain-2UniRule annotationAdd BLAST71
Regioni461 – 537Sigma-70 factor domain-3UniRule annotationAdd BLAST77
Regioni550 – 603Sigma-70 factor domain-4UniRule annotationAdd BLAST54

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili361 – 381Sequence analysisAdd BLAST21
Coiled coili576 – 596Sequence analysisAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi406 – 409Interaction with polymerase core subunit RpoCUniRule annotation4

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

Gene3Di1.10.10.10, 2 hits
HAMAPiMF_00963 Sigma70_RpoD_SigA, 1 hit
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR000943 RNA_pol_sigma70
IPR009042 RNA_pol_sigma70_r1_2
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR007631 RNA_pol_sigma_70_non-ess
IPR007127 RNA_pol_sigma_70_r1_1
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR012760 RNA_pol_sigma_RpoD_C
IPR028630 Sigma70_RpoD
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF04546 Sigma70_ner, 1 hit
PF03979 Sigma70_r1_1, 1 hit
PF00140 Sigma70_r1_2, 1 hit
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 1 hit
TIGRFAMsiTIGR02393 RpoD_Cterm, 1 hit
TIGR02937 sigma70-ECF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

O87812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKAQQQSR IKELIVLGRE QGYLTYAEVN DHLPEDISDP EQVEDIIRMI
60 70 80 90 100
NDMGINVFEA APDKDSLMLA DADTDEAAAE EAAAALAAVK TDIGRTTDPV
110 120 130 140 150
RMYMREMGTV ELLTREGEIG IAKRIEEGIR EVMGAIAHFP GTVDRILSEY
160 170 180 190 200
NRVTTEGGRL SDVLSGYIDP DDGIAPPAAE VPPPVDAKAA KADDDSEDDD
210 220 230 240 250
AESSGDDEDE VESGPDPIIA AQRFGAVSDQ MEITRKALKK HGRTNKQAIA
260 270 280 290 300
ELIALAELFM PIKLVPKQFE GLVERVRSAL ERLRAQERAI MQLCVRDARM
310 320 330 340 350
PRADFLRQFP GNEVDESWTD ALAKGKAKYA EAIGRLQPDI IRCQQKLTAL
360 370 380 390 400
ETETGLTIAE IKDINRRMSI GEAKARRAKK EMVEANLRLV ISIAKKYTNR
410 420 430 440 450
GLQFLDLIQE GNIGLMKAVD KFEYRRGYKF STYATWWIRQ AITRSIADQA
460 470 480 490 500
RTIRIPVHMI ETINKLNRIS RQMLQEMGRE PTPEELGERM EMPEDKIRKV
510 520 530 540 550
LKIAKEPISM ETPIGDDEDS HLGDFIEDST MQSPIDVATV ESLKEATRDV
560 570 580 590 600
LSGLTAREAK VLRMRFGIDM NTDHTLEEVG KQFDVTRERI RQIEAKALRK
610
LRHPTRSEHL RSFLDE
Length:616
Mass (Da):69,253
Last modified:November 1, 1998 - v1
Checksum:iF35507E857D0B988
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007830 Genomic DNA Translation: CAA07705.1

Similar proteinsi

Entry informationi

Entry nameiO87812_PSETO
AccessioniPrimary (citable) accession number: O87812
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health