O87802 (O87802_PSEST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Toluene, o-xylene monooxygenase oxygenase subunit EMBL CAA06658.1

Gene names

Name:

touE EMBL CAA06658.1

Organism

Pseudomonas stutzeri (Pseudomonas perfectomarina) EMBL CAA06658.1

Taxonomic identifier

316 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Monooxygenase EMBL CAA06658.1 Oxidoreductase
Technical term	3D-structure PDB 1T0R PDB 1T0Q PDB 1T0S PDB 2IND PDB 2INC PDB 2RDB
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: InterPro transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O87802 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 09F9A3180112D88B	FASTA	330	38,334
	10 20 30 40 50 60 MSEQQPEALK PLKTWSHLAG NRRRPSEYEV VSTNLHYFTD NPERPWELDS NLPMQTWYKK 70 80 90 100 110 120 YCFDSPLKHD DWNAFRDPDQ LVYRTYNLLQ DGQESYVQGL FDQLNDRGHD QMLTREWVET 130 140 150 160 170 180 LARFYTPARY LFHALQMGSV YIHQIAPAST ITNCATYETA DHLRWLTHTA YRTRELANCY 190 200 210 220 230 240 PDVGFGKRER DVWENDPAWQ GFRELIEKAL IAWDWGEAFT AINLVTKPAV EEALLQQLGS 250 260 270 280 290 300 LAQSEGDTLL GLLAQAQKRD AERHRRWSSA LVKMALEKEG NREVLQKWVA KWEPLADKAI 310 320 330 EAYCSALPDG ENAIVEAKSA SRYVRQMMGL

References

[1]	"Analysis of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1." Bertoni G., Martino M., Galli E., Barbieri P. Appl. Environ. Microbiol. 64:3626-3632(1998) [PubMed: 9758777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: OX1 EMBL CAA06658.1.
[2]	"Identification of the Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase regulatory gene (touR) and of its cognate promoter." Arenghi F.L., Pinti M., Galli E., Barbieri P. Appl. Environ. Microbiol. 65:4057-4063(1999) [PubMed: 10473416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: OX1 EMBL CAA06658.1.
[3]	Bertoni G. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: OX1 EMBL CAA06658.1.
[4]	"Crystal structure of the toluene/o-xylene monooxygenase hydroxylase from Pseudomonas stutzeri OX1. Insight into the substrate specificity, substrate channeling, and active site tuning of multicomponent monooxygenases." Sazinsky M.H., Bard J., Di Donato A., Lippard S.J. J. Biol. Chem. 279:30600-30610(2004) [PubMed: 15096510] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
[5]	"X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior." McCormick M.S., Sazinsky M.H., Condon K.L., Lippard S.J. J. Am. Chem. Soc. 128:15108-15110(2006) [PubMed: 17117860] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 8-329.
[6]	"Dioxygen activation at non-heme diiron centers: oxidation of a proximal residue in the I100W variant of toluene/o-xylene monooxygenase hydroxylase." Murray L.J., Garcia-Serres R., McCormick M.S., Davydov R., Naik S.G., Kim S.H., Hoffman B.M., Huynh B.H., Lippard S.J. Biochemistry 46:14795-14809(2007) [PubMed: 18044971] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ005663 Genomic DNA. Translation: CAA06658.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T0Q	X-ray	2.15	B	1-330	[»]
1T0R	X-ray	2.30	B	1-330	[»]
1T0S	X-ray	2.20	B	1-330	[»]
2INC	X-ray	1.85	B	8-329	[»]
2IND	X-ray	2.20	B	8-330	[»]
2RDB	X-ray	2.10	B	1-330	[»]

ProteinModelPortal

O87802.

SMR

O87802. Positions 8-330.

ModBase

Search...

Protein-protein interaction databases

IntAct

O87802. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009078. Ferritin/RR-like.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. Ribncl_red-rel.
[Graphical view]

Gene3D

G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.

Pfam

PF02332. Phenol_Hydrox. 1 hit.
[Graphical view]

PIRSF

PIRSF000040. MMOH_comp. 1 hit.

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O87802_PSEST

Accession

Primary (citable) accession number: O87802

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	October 19, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)