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Protein
Submitted name:

Toluene o-xylene monooxygenase component TouE

Gene

touE

Organism
Pseudomonas stutzeri (Pseudomonas perfectomarina)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

MonooxygenaseImported, Oxidoreductase

Names & Taxonomyi

Protein namesi
Submitted name:
Toluene o-xylene monooxygenase component TouEImported
Submitted name:
Toluene, o-xylene monooxygenase oxygenase subunitImported
Gene namesi
Name:touEImported
OrganismiPseudomonas stutzeri (Pseudomonas perfectomarina)Imported
Taxonomic identifieri316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Interactioni

Protein-protein interaction databases

DIPiDIP-37864N.
IntActiO87802. 1 interaction.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T0QX-ray2.15B1-330[»]
1T0RX-ray2.30B1-330[»]
1T0SX-ray2.20B1-330[»]
2INCX-ray1.85B8-329[»]
2INDX-ray2.20B8-330[»]
2RDBX-ray2.10B1-330[»]
ProteinModelPortaliO87802.
SMRiO87802. Positions 8-330.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO87802.

Family & Domainsi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF02332. Phenol_Hydrox. 1 hit.
[Graphical view]
PIRSFiPIRSF000040. MMOH_comp. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

O87802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQQPEALK PLKTWSHLAG NRRRPSEYEV VSTNLHYFTD NPERPWELDS
60 70 80 90 100
NLPMQTWYKK YCFDSPLKHD DWNAFRDPDQ LVYRTYNLLQ DGQESYVQGL
110 120 130 140 150
FDQLNDRGHD QMLTREWVET LARFYTPARY LFHALQMGSV YIHQIAPAST
160 170 180 190 200
ITNCATYETA DHLRWLTHTA YRTRELANCY PDVGFGKRER DVWENDPAWQ
210 220 230 240 250
GFRELIEKAL IAWDWGEAFT AINLVTKPAV EEALLQQLGS LAQSEGDTLL
260 270 280 290 300
GLLAQAQKRD AERHRRWSSA LVKMALEKEG NREVLQKWVA KWEPLADKAI
310 320 330
EAYCSALPDG ENAIVEAKSA SRYVRQMMGL
Length:330
Mass (Da):38,334
Last modified:November 1, 1998 - v1
Checksum:i09F9A3180112D88B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KT935509 Genomic DNA. Translation: ALP69208.1.
AJ005663 Genomic DNA. Translation: CAA06658.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KT935509 Genomic DNA. Translation: ALP69208.1.
AJ005663 Genomic DNA. Translation: CAA06658.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T0QX-ray2.15B1-330[»]
1T0RX-ray2.30B1-330[»]
1T0SX-ray2.20B1-330[»]
2INCX-ray1.85B8-329[»]
2INDX-ray2.20B8-330[»]
2RDBX-ray2.10B1-330[»]
ProteinModelPortaliO87802.
SMRiO87802. Positions 8-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-37864N.
IntActiO87802. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO87802.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF02332. Phenol_Hydrox. 1 hit.
[Graphical view]
PIRSFiPIRSF000040. MMOH_comp. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1."
    Bertoni G., Martino M., Galli E., Barbieri P.
    Appl. Environ. Microbiol. 64:3626-3632(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: OX1Imported.
  2. "Identification of the Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase regulatory gene (touR) and of its cognate promoter."
    Arenghi F.L.G., Pinti M., Galli E., Barbieri P.
    Appl. Environ. Microbiol. 65:4057-4063(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: OX1Imported.
  3. Bertoni G.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: OX1Imported.
  4. "Crystal structure of the toluene/o-xylene monooxygenase hydroxylase from Pseudomonas stutzeri OX1. Insight into the substrate specificity, substrate channeling, and active site tuning of multicomponent monooxygenases."
    Sazinsky M.H., Bard J., Di Donato A., Lippard S.J.
    J. Biol. Chem. 279:30600-30610(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
  5. "X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior."
    McCormick M.S., Sazinsky M.H., Condon K.L., Lippard S.J.
    J. Am. Chem. Soc. 128:15108-15110(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 8-329.
  6. "Dioxygen activation at non-heme diiron centers: oxidation of a proximal residue in the I100W variant of toluene/o-xylene monooxygenase hydroxylase."
    Murray L.J., Garcia-Serres R., McCormick M.S., Davydov R., Naik S.G., Kim S.H., Hoffman B.M., Huynh B.H., Lippard S.J.
    Biochemistry 46:14795-14809(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).
  7. "Functional redundancy of multicomponent monooxygenase genes is achieved by unified genetic regulation and expression of different catabolic operons in indigenous phenol- and toluene-degrading bacteria."
    Heinaru E., Naanuri E., Joesaar M., Grunbach M., Heinaru A.
    Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2A20Imported.

Entry informationi

Entry nameiO87802_PSEST
AccessioniPrimary (citable) accession number: O87802
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: April 13, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.