O87798 (O87798_PSEST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Toluene, o-xylene monooxygenase oxygenase subunit EMBL CAA06654.1

Gene names

Name:

touA EMBL CAA06654.1

Organism

Pseudomonas stutzeri (Pseudomonas perfectomarina) EMBL CAA06654.1

Taxonomic identifier

316 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Iron PDB 2RDB PDB 1T0Q PDB 2INC PDB 1T0R PDB 1T0S Metal-binding PDB 2RDB PDB 1T0Q PDB 2INC PDB 1T0R PDB 1T0S
Molecular function	Monooxygenase EMBL CAA06654.1 Oxidoreductase
Technical term	3D-structure PDB 2RDB PDB 1T0Q PDB 2INC PDB 1T0R PDB 1T0S PDB 2IND
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	104	1	Iron 1 PDB 2RDB PDB 1T0Q
	Metal binding	134	1	Iron 1 PDB 2RDB PDB 1T0Q
	Metal binding	134	1	Iron 2 PDB 2RDB PDB 2INC
	Metal binding	137	1	Iron 1; via pros nitrogen PDB 2RDB PDB 1T0Q
	Metal binding	197	1	Iron 2 PDB 2RDB PDB 2INC
	Metal binding	231	1	Iron 2 PDB 2RDB PDB 2INC
	Metal binding	234	1	Iron 2; via tele nitrogen PDB 2RDB PDB 2INC

Sequences

	Sequence		Length	Mass (Da)
	O87798 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E49B0C06D5BB43D8	FASTA	498	57,725
	10 20 30 40 50 60 MSMLKREDWY DLTRTTNWTP KYVTENELFP EEMSGARGIS MEAWEKYDEP YKITYPEYVS 70 80 90 100 110 120 IQREKDSGAY SIKAALERDG FVDRADPGWV STMQLHFGAI ALEEYAASTA EARMARFAKA 130 140 150 160 170 180 PGNRNMATFG MMDENRHGQI QLYFPYANVK RSRKWDWAHK AIHTNEWAAI AARSFFDDMM 190 200 210 220 230 240 MTRDSVAVSI MLTFAFETGF TNMQFLGLAA DAAEAGDHTF ASLISSIQTD ESRHAQQGGP 250 260 270 280 290 300 SLKILVENGK KDEAQQMVDV AIWRSWKLFS VLTGPIMDYY TPLESRNQSF KEFMLEWIVA 310 320 330 340 350 360 QFERQLLDLG LDKPWYWDQF MQDLDETHHG MHLGVWYWRP TVWWDPAAGV SPEEREWLEE 370 380 390 400 410 420 KYPGWNDTWG QCWDVITDNL VNGKPELTVP ETLPTICNMC NLPIAHTPGN KWNVKDYQLE 430 440 450 460 470 480 YEGRLYHFGS EADRWCFQID PERYKNHTNL VDRFLKGEIQ PADLAGALMY MSLEPGVMGD 490 DAHDYEWVKA YQKKTNAA

References

[1]	"Analysis of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1." Bertoni G., Martino M., Galli E., Barbieri P. Appl. Environ. Microbiol. 64:3626-3632(1998) [PubMed: 9758777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: OX1 EMBL CAA06654.1.
[2]	"Identification of the Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase regulatory gene (touR) and of its cognate promoter." Arenghi F.L., Pinti M., Galli E., Barbieri P. Appl. Environ. Microbiol. 65:4057-4063(1999) [PubMed: 10473416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: OX1 EMBL CAA06654.1.
[3]	Bertoni G. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: OX1 EMBL CAA06654.1.
[4]	"Crystal structure of the toluene/o-xylene monooxygenase hydroxylase from Pseudomonas stutzeri OX1. Insight into the substrate specificity, substrate channeling, and active site tuning of multicomponent monooxygenases." Sazinsky M.H., Bard J., Di Donato A., Lippard S.J. J. Biol. Chem. 279:30600-30610(2004) [PubMed: 15096510] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEX WITH IRON.
[5]	"X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior." McCormick M.S., Sazinsky M.H., Condon K.L., Lippard S.J. J. Am. Chem. Soc. 128:15108-15110(2006) [PubMed: 17117860] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 2-492 IN COMPLEX WITH IRON.
[6]	"Dioxygen activation at non-heme diiron centers: oxidation of a proximal residue in the I100W variant of toluene/o-xylene monooxygenase hydroxylase." Murray L.J., Garcia-Serres R., McCormick M.S., Davydov R., Naik S.G., Kim S.H., Hoffman B.M., Huynh B.H., Lippard S.J. Biochemistry 46:14795-14809(2007) [PubMed: 18044971] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH IRON.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ005663 Genomic DNA. Translation: CAA06654.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T0Q	X-ray	2.15	A	1-498	[»]
1T0R	X-ray	2.30	A	1-498	[»]
1T0S	X-ray	2.20	A	1-498	[»]
2INC	X-ray	1.85	A	2-492	[»]
2IND	X-ray	2.20	A	2-492	[»]
2RDB	X-ray	2.10	A	1-498	[»]

ProteinModelPortal

O87798.

SMR

O87798. Positions 2-492.

ModBase

Search...

Protein-protein interaction databases

IntAct

O87798. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009078. Ferritin/RR-like.
IPR003430. Phenol_Hydrox.
IPR012348. Ribncl_red-rel.
IPR007029. YHS.
[Graphical view]

Gene3D

G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.

Pfam

PF02332. Phenol_Hydrox. 1 hit.
PF04945. YHS. 1 hit.
[Graphical view]

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O87798_PSEST

Accession

Primary (citable) accession number: O87798

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	October 19, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)