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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi487MagnesiumUniRule annotation1
Metal bindingi493MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.7.8. 5092.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001979161 – 701Polyribonucleotide nucleotidyltransferaseAdd BLAST701

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO87792.
SMRiO87792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 613KHUniRule annotationAdd BLAST60
Domaini623 – 691S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR036345. ExoRNase_PH_dom2_sf.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR036612. KH_dom_type_1_sf.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom_sf.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

O87792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPVIKTFQF GQSTVTLETG RIARQATGAV LVTVDNDVTV LVTVVGAKQA
60 70 80 90 100
DPGKGFFPLS VHYQEKTYAA GKIPGGFFKR EGRPSEKETL TSRLIDRPIR
110 120 130 140 150
PLFPEGFMNE VQVVCTVVST SKKTDPDIAA MIGTSAALAI SGIPFEGPIG
160 170 180 190 200
AARVAFHEST GYLLNPTYEQ LAASSLDMVV AGTSDAVLMV ESEAQELTED
210 220 230 240 250
QMLGAVLFAH DEFQAVIQAV KELAAEAGKP TWDWKPAVAN TELFNAIRAE
260 270 280 290 300
FGEAVSQGYT ITVKADRYAR LGELRDQAVA KFSGEEGQPS ASEVKEIFGE
310 320 330 340 350
IEYRTVRENI VNGKPRIDGR DNKTVRPLNI EVGVLPKTHG SALFTRGETQ
360 370 380 390 400
ALVVATLGTA RDAQLLDTLE GEKKDPFMLH YNFPPFSVGE CGRMGGAGRR
410 420 430 440 450
EIGHGRLARR SVQAMLPAAD VFPYTIRVVS EITESNGSSS MASVCGASLA
460 470 480 490 500
LMDAGVPMKA PVAGIAMGLV KEGDKFAVLT DILGDEDHLG DMDFKVAGTA
510 520 530 540 550
KGVTALQMDI KINGITEEIM EIALGQALEA RLNILGQMNQ VIGQSRTELS
560 570 580 590 600
ANAPTMIAMK IDTDKIRDVI GKGGATIRAI CEETKASIDI EDDGSIKIFG
610 620 630 640 650
ETKEAADAAK QRILGITAEA EIGKIYVGKV ERIVDFGAFV NILPGKDGLV
660 670 680 690 700
HISMLSDARV EKVTDILKEG QEVEVLVLDV DNRGRIKLSI KDVAAAKASG

V
Length:701
Mass (Da):74,985
Last modified:November 1, 1998 - v1
Checksum:i5DB8F1A446DF0F2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18132 Genomic DNA. Translation: CAA77048.1.

Similar proteinsi

Entry informationi

Entry nameiPNP_PSEPU
AccessioniPrimary (citable) accession number: O87792
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program