O87765 (PCP_LACLM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrrolidone-carboxylate peptidase
EC=3.4.19.3

Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name=PGP-I
Short name=Pyrase

Gene names

Name:	pcp
Ordered Locus Names:	llmg_0663

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. cremoris

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes 5-oxoproline from various penultimate amino acid residues except L-proline By similarity. HAMAP-Rule MF_00417
Catalytic activity	Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro. HAMAP-Rule MF_00417
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase C15 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW pyroglutamyl-peptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Pyrrolidone-carboxylate peptidase HAMAP-Rule MF_00417		PRO_0000184722
Sites
	Active site	78	1	By similarity
	Active site	141	1	By similarity
	Active site	165	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O87765 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 70AE7E36E0882289	FASTA	215	23,519
	10 20 30 40 50 60 MKILVTGFDP FGDDKINPAI EAVKRLPDEI AGAQIVKLEI PTKFNVSADV VKDAIAKEKP 70 80 90 100 110 120 DYVLSIGQAG GRFELTPERV AINLDDGRIQ DNAGYQPLNH TIHGDDENAY FTQLPIKAMA 130 140 150 160 170 180 KAIREAGVPS AVSNTAGTYV CNHIFYQVQY MRDKMFPDIK AGFMHIPFLP EQVVTRPETP 190 200 210 ALSLDDDVLG ITAAIKAIVS RDGKGDIETI EGKDH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A natural large chromosomal inversion in Lactococcus lactis is mediated by homologous recombination between two insertion sequences." Daveran-Mingot M.L., Campo N., Ritzenthaler P., le Bourgeois P. J. Bacteriol. 180:4834-4842(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Pyrrolidone carboxyl peptidase of Lactococcus lactis MG1363." Buist G., Haandrikman A.J., Benus G., Feenstra B., Venema G., Kok J. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MG1363.
[4]	"Identification and characterisation of a gene encoding aminoacylase activity from Lactococcus lactis MG1363." Curley P., van Sinderen D. FEMS Microbiol. Lett. 183:177-182(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-215.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223960 Genomic DNA. Translation: CAA11691.1. AJ223962 Genomic DNA. Translation: CAA11699.1. AF323462 Genomic DNA. Translation: AAK20299.1. AM406671 Genomic DNA. Translation: CAL97264.1. AF168363 Genomic DNA. Translation: AAF36226.1.
RefSeq	YP_001032004.1. NC_009004.1.
3D structure databases
ProteinModelPortal	O87765.
ModBase	Search...
Protein-protein interaction databases
STRING	416870.llmg_0663.
Protein family/group databases
MEROPS	C15.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL97264; CAL97264; llmg_0663.
GeneID	4797823.
KEGG	llm:llmg_0663.
PATRIC	22282430. VBILacLac4574_0679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2039.
HOGENOM	HOG000242641.
KO	K01304.
OMA	AYFTQLP.
ProtClustDB	PRK13197.
Family and domain databases
Gene3D	3.40.630.20. 1 hit.
HAMAP	MF_00417. Pyrrolid_peptidase.
InterPro	IPR000816. Peptidase_C15. IPR016125. Peptidase_C15-like. [Graphical view]
PANTHER	PTHR23402. PTHR23402. 1 hit.
Pfam	PF01470. Peptidase_C15. 1 hit. [Graphical view]
PIRSF	PIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTS	PR00706. PYROGLUPTASE.
SUPFAM	SSF53182. Peptidase_C15-like. 1 hit.
TIGRFAMs	TIGR00504. pyro_pdase. 1 hit.
PROSITE	PS01334. PYRASE_CYS. 1 hit. PS01333. PYRASE_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCP_LACLM

Accession

Primary (citable) accession number: O87765
Secondary accession number(s): A2RJ15, Q9L9P5, Q9R805

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program