Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrrolidone-carboxylate peptidase

Gene

pcp

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Removes 5-oxoproline from various penultimate amino acid residues except L-proline.By similarity

Catalytic activityi

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781By similarity
Active sitei141 – 1411By similarity
Active sitei165 – 1651By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. pyroglutamyl-peptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-693-MONOMER.

Protein family/group databases

MEROPSiC15.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrrolidone-carboxylate peptidase (EC:3.4.19.3)
Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name:
PGP-I
Short name:
Pyrase
Gene namesi
Name:pcp
Ordered Locus Names:llmg_0663
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000364 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Pyrrolidone-carboxylate peptidasePRO_0000184722Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi416870.llmg_0663.

Structurei

3D structure databases

ProteinModelPortaliO87765.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C15 family.Curated

Phylogenomic databases

eggNOGiCOG2039.
HOGENOMiHOG000242641.
KOiK01304.
OMAiIKLEIPT.
OrthoDBiEOG6X1124.

Family and domain databases

Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 1 hit.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILVTGFDP FGDDKINPAI EAVKRLPDEI AGAQIVKLEI PTKFNVSADV
60 70 80 90 100
VKDAIAKEKP DYVLSIGQAG GRFELTPERV AINLDDGRIQ DNAGYQPLNH
110 120 130 140 150
TIHGDDENAY FTQLPIKAMA KAIREAGVPS AVSNTAGTYV CNHIFYQVQY
160 170 180 190 200
MRDKMFPDIK AGFMHIPFLP EQVVTRPETP ALSLDDDVLG ITAAIKAIVS
210
RDGKGDIETI EGKDH
Length:215
Mass (Da):23,519
Last modified:November 1, 1998 - v1
Checksum:i70AE7E36E0882289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223960 Genomic DNA. Translation: CAA11691.1.
AJ223962 Genomic DNA. Translation: CAA11699.1.
AF323462 Genomic DNA. Translation: AAK20299.1.
AM406671 Genomic DNA. Translation: CAL97264.1.
AF168363 Genomic DNA. Translation: AAF36226.1.
RefSeqiWP_011834666.1. NC_009004.1.
YP_001032004.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL97264; CAL97264; llmg_0663.
KEGGillm:llmg_0663.
PATRICi22282430. VBILacLac4574_0679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223960 Genomic DNA. Translation: CAA11691.1.
AJ223962 Genomic DNA. Translation: CAA11699.1.
AF323462 Genomic DNA. Translation: AAK20299.1.
AM406671 Genomic DNA. Translation: CAL97264.1.
AF168363 Genomic DNA. Translation: AAF36226.1.
RefSeqiWP_011834666.1. NC_009004.1.
YP_001032004.1. NC_009004.1.

3D structure databases

ProteinModelPortaliO87765.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_0663.

Protein family/group databases

MEROPSiC15.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL97264; CAL97264; llmg_0663.
KEGGillm:llmg_0663.
PATRICi22282430. VBILacLac4574_0679.

Phylogenomic databases

eggNOGiCOG2039.
HOGENOMiHOG000242641.
KOiK01304.
OMAiIKLEIPT.
OrthoDBiEOG6X1124.

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-693-MONOMER.

Family and domain databases

Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 1 hit.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A natural large chromosomal inversion in Lactococcus lactis is mediated by homologous recombination between two insertion sequences."
    Daveran-Mingot M.L., Campo N., Ritzenthaler P., le Bourgeois P.
    J. Bacteriol. 180:4834-4842(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Pyrrolidone carboxyl peptidase of Lactococcus lactis MG1363."
    Buist G., Haandrikman A.J., Benus G., Feenstra B., Venema G., Kok J.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.
  4. "Identification and characterisation of a gene encoding aminoacylase activity from Lactococcus lactis MG1363."
    Curley P., van Sinderen D.
    FEMS Microbiol. Lett. 183:177-182(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-215.

Entry informationi

Entry nameiPCP_LACLM
AccessioniPrimary (citable) accession number: O87765
Secondary accession number(s): A2RJ15, Q9L9P5, Q9R805
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: April 29, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.