Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CTP synthase

Gene

pyrG

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Contrary to other CTP synthases, the lactococcal enzyme is also able to convert dUTP to dCTP, but this reaction may not play a significant physiological role.

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate. Also activated by magnesium. Inhibited by CTP.

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei382 – 3821NucleophileBy similarity
Active sitei509 – 5091By similarity
Active sitei511 – 5111By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-480-MONOMER.
SABIO-RKO87761.
UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthase (EC:6.3.4.2)
Alternative name(s):
CTP synthetase
UTP--ammonia ligase
Gene namesi
Name:pyrG
Ordered Locus Names:llmg_0467
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535CTP synthasePRO_0000138192Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi416870.llmg_0467.

Structurei

3D structure databases

ProteinModelPortaliO87761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini293 – 535243Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 255255Aminator domainAdd
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiFDHNITT.
OrthoDBiEOG6RC3NR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKYIFVTG GGTSSMGKGI VAASLGRLLK NRGLKVTVQK FDPYLNIDPG
60 70 80 90 100
TMSPYQHGEV FVTDDGAETD LDLGHYERFI DINLNKYSNV TSGKVYSEIL
110 120 130 140 150
RKERKGEYLG ATVQMVPHVT NMLKEKIKRA ATTTDADIII TEVGGTVGDM
160 170 180 190 200
ESLPFIEALR QMKAEVGADN VMYIHTVPIL HLRAAGELKT KIAQNATKTL
210 220 230 240 250
REYGIQANML VLRSEVPITT EMRDKIAMFC DVAPEAVIQS LDVEHLYQIP
260 270 280 290 300
LNLQAQNMDQ IVCDHLKLDA PKADMAEWSA MVDHVMNLKK KVKIALVGKY
310 320 330 340 350
VELPDAYISV TEALKHAGYA SDAEVDINWV NANDVTDENV AELVGDAAGI
360 370 380 390 400
IVPGGFGQRG TEGKIAAIKY ARENDVPMLG ICLGMQLTAV EFARNVLGLE
410 420 430 440 450
GAHSFELDPE TKYPVIDIMR DQVDVEDMGG TLRLGLYPAK LKNGSRAKAA
460 470 480 490 500
YNDAEVVQRR HRHRYEFNNK YREDFEKAGF VFSGVSPDNR LVEIVELSGK
510 520 530
KFFVACQYHP ELQSRPNRPE ELYTEFIRVA VENSK
Length:535
Mass (Da):59,455
Last modified:October 1, 2000 - v2
Checksum:i045724E0FEDE5B4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010153 Genomic DNA. Translation: CAA09021.2.
AM406671 Genomic DNA. Translation: CAL97071.1.
RefSeqiWP_011834508.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL97071; CAL97071; llmg_0467.
KEGGillm:llmg_0467.
PATRICi22281985. VBILacLac4574_0478.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010153 Genomic DNA. Translation: CAA09021.2.
AM406671 Genomic DNA. Translation: CAL97071.1.
RefSeqiWP_011834508.1. NC_009004.1.

3D structure databases

ProteinModelPortaliO87761.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_0467.

Protein family/group databases

MEROPSiC26.964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL97071; CAL97071; llmg_0467.
KEGGillm:llmg_0467.
PATRICi22281985. VBILacLac4574_0478.

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiFDHNITT.
OrthoDBiEOG6RC3NR.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciLLAC416870:GCDT-480-MONOMER.
SABIO-RKO87761.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and verification of the Lactococcus lactis pyrG gene and characterization of the gene product, CTP synthase."
    Wadskov-Hansen S.L.L., Willemoes M., Martinussen J., Hammer K., Neuhard J., Larsen S.
    J. Biol. Chem. 276:38002-38009(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.

Entry informationi

Entry nameiPYRG_LACLM
AccessioniPrimary (citable) accession number: O87761
Secondary accession number(s): A2RIH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 1, 2000
Last modified: November 11, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.