Reviewed, UniProtKB/Swiss-Prot O87717 (CHEB1_CAUCR)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	CC_0436

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000157984
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	151 – 343	193	CheB-type methylesterase
Sites
	Active site	163	1	By similarity
	Active site	189	1	By similarity
	Active site	285	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O87717-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: AB46083B61D6A3BF	FASTA	344	35,729
	10 20 30 40 50 60 MAKIRVLVVD DSATMRSLIS AALNRDPDIE VVGGAGDPFE ARGMIKALNP DVVTLDIEMP 70 80 90 100 110 120 NMNGIDFLEK IMRLRPMPVV MVSTLTQAGA EMTLRALELG AVDCVGKPAD ATGTQEALAE 130 140 150 160 170 180 IVAKVKIAAR ASVRTNAGAA PTSAPTRRKD FMPSGDIVAI GSSTGGVEAL LSILQLFPET 190 200 210 220 230 240 CPPTVITQHM PATFTASFAA RLDRSSGAKV QEASDGALLE PGKVYVAPGG ATHLEVVRSA 250 260 270 280 290 300 GLRCRLVAGD PVSGHRPSVD VLFNSVAHAV GDKAVGVILT GMGRDGAQGL LTMRKAGAKT 310 320 330 340 LGQDEASCVV YGMPRSAFEI GAVERQVSLS SMGQSILDLA SARR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Caulobacter major chemotaxis operon." Alley M.R.K. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 19089 / CB15.
[2]	"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ006687 Genomic DNA. Translation: CAA07180.1. AE005673 Genomic DNA. Translation: AAK22423.1.
PIR	C87303.
RefSeq	NP_419255.1.
3D structure databases
HSSP	HSSP built from PDB template 1A2O based on UniProtKB P04042.
ModBase	Search...
Genome annotation databases
GeneID	942022.
GenomeReviews	Gene locus CC_0436 in contig AE005673_GR.
KEGG	ccr:CC_0436.
NMPDR	fig|190650.1.peg.435.
TIGR	CC_0436.
Phylogenomic databases
HOGENOM	O87717.
OMA	GKIYLAP.
Enzyme and pathway databases
BRENDA	3.1.1.61. 2191.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_CAUCR

Accession

Primary (citable) accession number: O87717

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)