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Protein

Cobalt-precorrin-4 C(11)-methyltransferase

Gene

cbiF

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cobalt-precorrin-4 = S-adenosyl-L-homocysteine + cobalt-precorrin-5A.1 Publication

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route).
Proteins known to be involved in the 10 steps of the subpathway in this organism are:
  1. Sirohydrochlorin cobaltochelatase (cbiX)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Cobalt-precorrin-4 C(11)-methyltransferase (cbiF)
  5. Cobalt-precorrin-5A hydrolase (cbiG)
  6. Cobalt-precorrin-5B C(1)-methyltransferase (cbiD), Cobalt-precorrin-5B C(1)-methyltransferase (cbiD)
  7. Cobalt-precorrin-6A reductase (cbiJ)
  8. Cobalamin biosynthesis bifunctional protein CbiET (cbiET)
  9. Cobalt-precorrin-8 methylmutase (cbiC)
  10. Cobyrinate a,c-diamide synthase (cbiA), Cobyrinate a,c-diamide synthase (cbiA)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.271. 656.
UniPathwayiUPA00148; UER00226.

Names & Taxonomyi

Protein namesi
Recommended name:
Cobalt-precorrin-4 C(11)-methyltransferase (EC:2.1.1.271)
Alternative name(s):
Cobalt-precorrin-3 methylase
Gene namesi
Name:cbiF
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001503921 – 258Cobalt-precorrin-4 C(11)-methyltransferaseAdd BLAST258

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi545693.BMQ_2612.

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 7Combined sources7
Beta strandi9 – 11Combined sources3
Helixi13 – 15Combined sources3
Helixi18 – 26Combined sources9
Beta strandi28 – 32Combined sources5
Turni34 – 36Combined sources3
Helixi39 – 42Combined sources4
Beta strandi50 – 53Combined sources4
Helixi59 – 70Combined sources12
Turni71 – 73Combined sources3
Beta strandi76 – 82Combined sources7
Turni84 – 87Combined sources4
Helixi91 – 99Combined sources9
Beta strandi103 – 107Combined sources5
Helixi112 – 119Combined sources8
Turni127 – 129Combined sources3
Beta strandi133 – 137Combined sources5
Beta strandi140 – 142Combined sources3
Helixi146 – 148Combined sources3
Helixi150 – 154Combined sources5
Beta strandi158 – 164Combined sources7
Helixi166 – 168Combined sources3
Helixi169 – 178Combined sources10
Beta strandi186 – 192Combined sources7
Beta strandi199 – 204Combined sources6
Helixi205 – 207Combined sources3
Helixi208 – 214Combined sources7
Beta strandi219 – 226Combined sources8
Helixi227 – 229Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CBFX-ray2.40A1-250[»]
2CBFX-ray3.10A1-231[»]
ProteinModelPortaliO87696.
SMRiO87696.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO87696.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi115 – 122Poly-Ala8
Compositional biasi187 – 190Poly-Val4

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105CIA. Bacteria.
COG2875. LUCA.

Family and domain databases

CDDicd11641. Precorrin-4_C11-MT. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006362. Cbl_synth_CobM/CibF.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01465. cobM_cbiF. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLYIIGAGP GDPDLITVKG LKLLQQADVV LYADSLVSQD LIAKSKPGAE
60 70 80 90 100
VLKTAGMHLE EMVGTMLDRM REGKMVVRVH TGDPAMYGAI MEQMVLLKRE
110 120 130 140 150
GVDIEIVPGV TSVFAAAAAA EAELTIPDLT QTVILTRAEG RTPVPEFEKL
160 170 180 190 200
TDLAKHKCTI ALFLSATLTK KVMKEFINAG WSEDTPVVVV YKATWPDEKI
210 220 230 240 250
VRTTVKDLDD AMRTNGIRKQ AMILAGWALD PHIHDKDYRS KLYDKTFTHG

FRKGVKSE
Length:258
Mass (Da):28,452
Last modified:November 1, 1998 - v1
Checksum:i59C2E7FF9FAF1D03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000758 Genomic DNA. Translation: CAA04314.1.
PIRiT44690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000758 Genomic DNA. Translation: CAA04314.1.
PIRiT44690.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CBFX-ray2.40A1-250[»]
2CBFX-ray3.10A1-231[»]
ProteinModelPortaliO87696.
SMRiO87696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi545693.BMQ_2612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CIA. Bacteria.
COG2875. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00226.
BRENDAi2.1.1.271. 656.

Miscellaneous databases

EvolutionaryTraceiO87696.

Family and domain databases

CDDicd11641. Precorrin-4_C11-MT. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006362. Cbl_synth_CobM/CibF.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01465. cobM_cbiF. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBIF_BACME
AccessioniPrimary (citable) accession number: O87696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.