Reviewed, UniProtKB/Swiss-Prot O87696 (CBIF_BACME)

Names and origin

Protein names

Recommended name:
    Cobalt-precorrin-4 C(11)-methyltransferase
    EC=2.1.1.-
Alternative name(s):
    Cobalt-precorrin-3 methylase

Gene names

Name:

cbiF

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5.
Catalytic activity	S-adenosyl-L-methionine + cobalt-precorrin-4 = S-adenosyl-L-homocysteine + cobalt-precorrin-5.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinic acid a,c-diamide from sirohydrochlorin (anaerobic route): step 4/10.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	precorrin-4 C11-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 258

258

Cobalt-precorrin-4 C(11)-methyltransferase

PRO_0000150392

Regions

Compositional bias

115 – 122

8

Poly-Ala

Compositional bias

187 – 190

4

Poly-Val

Secondary structure

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258

Helix Strand Turn

Details...

Beta strand

1 – 7

7

Beta strand

9 – 11

3

Helix

13 – 15

3

Helix

18 – 26

9

Beta strand

28 – 32

5

Turn

34 – 36

3

Helix

39 – 42

4

Beta strand

50 – 53

4

Helix

59 – 70

12

Turn

71 – 73

3

Beta strand

76 – 82

7

Turn

84 – 87

4

Helix

91 – 99

9

Beta strand

103 – 107

5

Helix

112 – 119

8

Turn

127 – 129

3

Beta strand

133 – 137

5

Beta strand

140 – 142

3

Helix

146 – 148

3

Helix

150 – 154

5

Beta strand

158 – 164

7

Helix

169 – 178

10

Beta strand

186 – 192

7

Beta strand

199 – 204

6

Helix

205 – 207

3

Helix

208 – 214

7

Beta strand

219 – 226

8

Helix

227 – 229

3

Sequences

	Sequence		Length	Mass (Da)
	O87696-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 59C2E7FF9FAF1D03	FASTA	258	28,452
	10 20 30 40 50 60 MKLYIIGAGP GDPDLITVKG LKLLQQADVV LYADSLVSQD LIAKSKPGAE VLKTAGMHLE 70 80 90 100 110 120 EMVGTMLDRM REGKMVVRVH TGDPAMYGAI MEQMVLLKRE GVDIEIVPGV TSVFAAAAAA 130 140 150 160 170 180 EAELTIPDLT QTVILTRAEG RTPVPEFEKL TDLAKHKCTI ALFLSATLTK KVMKEFINAG 190 200 210 220 230 240 WSEDTPVVVV YKATWPDEKI VRTTVKDLDD AMRTNGIRKQ AMILAGWALD PHIHDKDYRS 250 KLYDKTFTHG FRKGVKSE

References

[1]	"Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon." Raux E., Lanois A., Warren M.J., Rambach A., Thermes C. Biochem. J. 335:159-166(1998) [PubMed: 9742225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 509 / IFO 12109.
[2]	"Cobalamin (vitamin B12) biosynthesis -- cloning, expression and crystallisation of the Bacillus megaterium S-adenosyl-L-methionine-dependent cobalt-precorrin-4 transmethylase CbiF." Raux E., Schubert H.L., Woodcock S.C., Wilson K.S., Warren M.J. Eur. J. Biochem. 254:341-346(1998) [PubMed: 9660189] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ000758 Genomic DNA. Translation: CAA04314.1.

PIR

T44690.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CBF	X-ray	2.40	A	1-231	[»]
2CBF	X-ray	3.10	A	1-231	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006362. Cbl_synth_CobM/CibF_core.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit.
G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit.

Pfam

PF00590. TP_methylase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01465. cobM_cbiF. 1 hit.

PROSITE

PS00839. SUMT_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CBIF_BACME

Accession

Primary (citable) accession number: O87696

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)