Reviewed, UniProtKB/Swiss-Prot O87612 (CLCE_PSEAE)

Names and origin

Protein names

Recommended name:
    Maleylacetate reductase
    EC=1.3.1.32

Gene names

Name:

clcE

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-oxoadipate + NAD(P)+ = 2-maleylacetate + NAD(P)H.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	maleylacetate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Maleylacetate reductase		PRO_0000087845

Sequences

	Sequence		Length	Mass (Da)
	O87612-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D4CE1DD97D830E79	FASTA	352	37,755
	10 20 30 40 50 60 MNFIHDYRSP RVIFGPDSLA RLPQELERLG IDRALVLTTP EQAPLGRQVA EPVIGHVAAF 70 80 90 100 110 120 YDGATMHVPA LVAEEACKIA RTSEANGVIA IGGGSTIGLA KIVALRTELP IVAVPTTYAG 130 140 150 160 170 180 SEMTSIFGIT EGGVKKTGRD ARVMPRAVIY EPRLTLELPL SISVTSAINA IAHAVEGLYA 190 200 210 220 230 240 PDATPLLTIM AQEGIAATVR AISRMYQSPR DLQARGDALY GAWLCASVVG NVSMALHHKL 250 260 270 280 290 300 CHTLGGTLDL PHAQTHTVVL PHALAYNARA VPDAMRVLRI ALGHDDPPTA LYELARDNGA 310 320 330 340 350 PVALRDLGMR EEDIEHVGDL ALQDRYPNPR ELDRDALLAL LRDAYHGRPP SA

References

[1]

"Cloning, nucleotide sequencing, and functional analysis of a novel, mobile cluster of biodegradation genes from Pseudomonas aeruginosa strain JB2." Hickey W.J., Sabat G., Yuroff A.S., Arment A.R., Perez-Lesher J. Appl. Environ. Microbiol. 67:4603-4609(2001) [PubMed: 11571162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JB2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF087482 Genomic DNA. Translation: AAC69478.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.32. 354.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLCE_PSEAE

Accession

Primary (citable) accession number: O87612

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)