O87605 (O87605_9ACTO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cytochrome P450 hydroxylase PiKC EMBL AAC68886.1

Submitted name:
Cytochrome P450 monooxygenase EMBL AAC64105.1

Gene names

Name:	picK EMBL AAC64105.1
Synonyms:	pikC EMBL AAC68886.1

Organism

Streptomyces venezuelae EMBL AAC64105.1

Taxonomic identifier

54571 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the cytochrome P450 family. RuleBase RU000461

Ontologies

Keywords
Ligand	Heme SAAS SAAS002397 RuleBase RU000461 PDB 2C7X PDB 2VZM PDB 2WI9 PDB 2WHW PDB 2BVJ PDB 2CA0 PDB 2C6H PDB 2VZ7 PDB 2CD8 Iron Metal-binding
Molecular function	Monooxygenase SAAS SAAS002397 RuleBase RU003700 EMBL AAC64105.1 Oxidoreductase
Technical term	3D-structure PDB 2C7X PDB 2VZM PDB 2WI9 PDB 2WHW PDB 2BVJ PDB 2CA0 PDB 2C6H PDB 2VZ7 PDB 2CD8
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	354	1	Iron (heme axial ligand) PDB 2C7X PDB 2VZM PDB 2WI9 PDB 2WHW PDB 2BVJ PDB 2CA0 PDB 2C6H PDB 2VZ7 PDB 2CD8

Sequences

	Sequence		Length	Mass (Da)
	O87605 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: B7392C742045F06B	FASTA	416	46,038
	10 20 30 40 50 60 MRRTQQGTTA SPPVLDLGAL GQDFAADPYP TYARLRAEGP AHRVRTPEGD EVWLVVGYDR 70 80 90 100 110 120 ARAVLADPRF SKDWRNSTTP LTEAEAALNH NMLESDPPRH TRLRKLVARE FTMRRVELLR 130 140 150 160 170 180 PRVQEIVDGL VDAMLAAPDG RADLMESLAW PLPITVISEL LGVPEPDRAA FRVWTDAFVF 190 200 210 220 230 240 PDDPAQAQTA MAEMSGYLSR LIDSKRGQDG EDLLSALVRT SDEDGSRLTS EELLGMAHIL 250 260 270 280 290 300 LVAGHETTVN LIANGMYALL SHPDQLAALR ADMTLLDGAV EEMLRYEGPV ESATYRFPVE 310 320 330 340 350 360 PVDLDGTVIP AGDTVLVVLA DAHRTPERFP DPHRFDIRRD TAGHLAFGHG IHFCIGAPLA 370 380 390 400 410 RLEARIAVRA LLERCPDLAL DVSPGELVWY PNPMIRGLKA LPIRWRRGRE AGRRTG

References

[1]	"Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae." Xue Y., Wilson D., Zhao L., Liu H., Sherman D.H. Chem. Biol. 5:661-667(1998) [PubMed: 9831532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15439 EMBL AAC68886.1.
[2]	"The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae." Sherman D.H., Li S., Yermalitskaya L.V., Kim Y., Smith J.A., Waterman M.R., Podust L.M. J. Biol. Chem. 281:26289-26297(2006) [PubMed: 16825192] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH HEME.
[3]	"Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae." Li S., Ouellet H., Sherman D.H., Podust L.M. J. Biol. Chem. 284:5723-5730(2009) [PubMed: 19124459] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH HEME.
[4]	"Selective oxidation of carbolide C-H bonds by an engineered macrolide P450 mono-oxygenase." Li S., Chaulagain M.R., Knauff A.R., Podust L.M., Montgomery J., Sherman D.H. Proc. Natl. Acad. Sci. U.S.A. 106:18463-18468(2009) [PubMed: 19833867] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH HEME.
[5]	"A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity." Xue Y., Zhao L., Liu H.W., Sherman D.H. Proc. Natl. Acad. Sci. U.S.A. 95:12111-12116(1998) [PubMed: 9770448] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15439 EMBL AAC68886.1.
[6]	"Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin." Betlach M.C., Kealey J.T., Ashley G.W., McDaniel R. Biochemistry 37:14937-14942(1998) [PubMed: 9778370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15439 EMBL AAC64105.1.
[7]	"Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc (Cyp107L1)." Yermalitskaya L.I., Kim Y., Sherman D.H., Waterman M.R., Podust L.M. Submitted (DEC-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) IN COMPLEX WITH HEME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF087022 Genomic DNA. Translation: AAC64105.1.
AF079139 Genomic DNA. Translation: AAC68886.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BVJ	X-ray	2.10	A/B	1-416	[»]
2C6H	X-ray	2.35	A/B	1-416	[»]
2C7X	X-ray	1.75	A	1-416	[»]
2CA0	X-ray	2.85	A/B	1-416	[»]
2CD8	X-ray	1.70	A/B	1-416	[»]
2VZ7	X-ray	3.20	A/B	1-416	[»]
2VZM	X-ray	1.85	A/B	1-416	[»]
2WHW	X-ray	2.20	A/B	1-416	[»]
2WI9	X-ray	2.00	A/B	1-416	[»]

ProteinModelPortal

O87605.

SMR

O87605. Positions 11-406.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]

Gene3D

G3DSA:1.10.630.10. Cyt_P450. 1 hit.

Pfam

PF00067. p450. 1 hit.
[Graphical view]

PRINTS

PR00359. BP450.
PR00385. P450.

SUPFAM

SSF48264. Cytochrome_P450. 1 hit.

PROSITE

PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

O87605_9ACTO

Accession

Primary (citable) accession number: O87605

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	July 27, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)