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O87405

- GLSA_RHIEC

UniProt

O87405 - GLSA_RHIEC

Protein

Thermolabile glutaminase

Gene

glsA

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamine + H2O = L-glutamate + NH3.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641SubstrateBy similarity
    Binding sitei114 – 1141SubstrateBy similarity
    Binding sitei160 – 1601SubstrateBy similarity
    Binding sitei167 – 1671SubstrateBy similarity
    Binding sitei191 – 1911SubstrateBy similarity
    Binding sitei243 – 2431SubstrateBy similarity
    Binding sitei261 – 2611Substrate; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. glutaminase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glutamine metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciRETL347834:GJJ0-2322-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thermolabile glutaminase (EC:3.5.1.2)
    Alternative name(s):
    Glutaminase A
    Gene namesi
    Name:glsA
    Ordered Locus Names:RHE_CH02311
    OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
    Taxonomic identifieri347834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000001936: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Thermolabile glutaminasePRO_0000110619Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi347834.RHE_CH02311.

    Structurei

    3D structure databases

    ProteinModelPortaliO87405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.Curated

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    KOiK01425.
    OMAiQRARRIN.
    OrthoDBiEOG6N94BK.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O87405-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLQATLDS IYTDILPRIG EGKVADYIPE LAKIDPRQFG MAIVTVDGQV    50
    FRVGDADIAF SIQSISKVFM LTLALGKVGE GLWKRVGREP SGSAFNSIVQ 100
    LEHESGIPRN PFINAGAIAV TDVVMAGHAP REAIGELLRF VRYLADDESI 150
    TIDDKVARSE TQTGYRNVAL ANFMRAYRNL DHPVDHVLGV YFHQCALAMS 200
    CEQLARAGLF LAARGSNPMT GHSVVSPKRA RRINALMLTC GHYDGSGDFA 250
    YHVGLPGKSG VGGGIFAVAP GIASIAVWSP GLNKVGNSQL GAVALEMLAA 300
    RTGWSVFGD 309
    Length:309
    Mass (Da):32,987
    Last modified:May 16, 2006 - v2
    Checksum:iCC678FB5E6A048FD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti94 – 941A → T in AAC63991. (PubMed:10095071)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057158 Genomic DNA. Translation: AAC63991.1.
    CP000133 Genomic DNA. Translation: ABC91091.1.
    RefSeqiYP_469818.1. NC_007761.1.

    Genome annotation databases

    EnsemblBacteriaiABC91091; ABC91091; RHE_CH02311.
    GeneIDi3894584.
    KEGGiret:RHE_CH02311.
    PATRICi23086148. VBIRhiEtl108884_2727.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057158 Genomic DNA. Translation: AAC63991.1 .
    CP000133 Genomic DNA. Translation: ABC91091.1 .
    RefSeqi YP_469818.1. NC_007761.1.

    3D structure databases

    ProteinModelPortali O87405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 347834.RHE_CH02311.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC91091 ; ABC91091 ; RHE_CH02311 .
    GeneIDi 3894584.
    KEGGi ret:RHE_CH02311.
    PATRICi 23086148. VBIRhiEtl108884_2727.

    Phylogenomic databases

    eggNOGi COG2066.
    HOGENOMi HOG000216890.
    KOi K01425.
    OMAi QRARRIN.
    OrthoDBi EOG6N94BK.

    Enzyme and pathway databases

    BioCyci RETL347834:GJJ0-2322-MONOMER.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    HAMAPi MF_00313. Glutaminase.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view ]
    PANTHERi PTHR12544. PTHR12544. 1 hit.
    Pfami PF04960. Glutaminase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding a thermolabile glutaminase."
      Calderon J., Huerta-Saquero A., Du Pont G., Duran S.
      Biochim. Biophys. Acta 1444:451-456(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFN 42 / ATCC 51251.
    3. Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiGLSA_RHIEC
    AccessioniPrimary (citable) accession number: O87405
    Secondary accession number(s): Q2K7U5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3