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O87405 (GLSA_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thermolabile glutaminase

EC=3.5.1.2
Alternative name(s):
Glutaminase A
Gene names
Name:glsA
Ordered Locus Names:RHE_CH02311
OrganismRhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]
Taxonomic identifier347834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00313

Sequence similarities

Belongs to the glutaminase family.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Thermolabile glutaminase HAMAP-Rule MF_00313
PRO_0000110619

Sites

Binding site641Substrate By similarity
Binding site1141Substrate By similarity
Binding site1601Substrate By similarity
Binding site1671Substrate By similarity
Binding site1911Substrate By similarity
Binding site2431Substrate By similarity
Binding site2611Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict941A → T in AAC63991. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O87405 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: CC678FB5E6A048FD

FASTA30932,987
        10         20         30         40         50         60 
MADLQATLDS IYTDILPRIG EGKVADYIPE LAKIDPRQFG MAIVTVDGQV FRVGDADIAF 

        70         80         90        100        110        120 
SIQSISKVFM LTLALGKVGE GLWKRVGREP SGSAFNSIVQ LEHESGIPRN PFINAGAIAV 

       130        140        150        160        170        180 
TDVVMAGHAP REAIGELLRF VRYLADDESI TIDDKVARSE TQTGYRNVAL ANFMRAYRNL 

       190        200        210        220        230        240 
DHPVDHVLGV YFHQCALAMS CEQLARAGLF LAARGSNPMT GHSVVSPKRA RRINALMLTC 

       250        260        270        280        290        300 
GHYDGSGDFA YHVGLPGKSG VGGGIFAVAP GIASIAVWSP GLNKVGNSQL GAVALEMLAA 


RTGWSVFGD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding a thermolabile glutaminase."
Calderon J., Huerta-Saquero A., Du Pont G., Duran S.
Biochim. Biophys. Acta 1444:451-456(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons."
Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G.
Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.
[3]"Identification of two glutaminases in Rhizobium etli."
Duran S., Sanchez-Linares L., Huerta-Saquero A., Du Pont G., Huerta-Zepeda A., Calderon J.
Biochem. Genet. 34:453-465(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF057158 Genomic DNA. Translation: AAC63991.1.
CP000133 Genomic DNA. Translation: ABC91091.1.
RefSeqYP_469818.1. NC_007761.1.

3D structure databases

ProteinModelPortalO87405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING347834.RHE_CH02311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC91091; ABC91091; RHE_CH02311.
GeneID3894584.
KEGGret:RHE_CH02311.
PATRIC23086148. VBIRhiEtl108884_2727.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2066.
HOGENOMHOG000216890.
KOK01425.
OMAQRARRIN.
OrthoDBEOG6N94BK.

Enzyme and pathway databases

BioCycRETL347834:GJJ0-2322-MONOMER.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLSA_RHIEC
AccessionPrimary (citable) accession number: O87405
Secondary accession number(s): Q2K7U5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families