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Protein

Thermolabile glutaminase

Gene

glsA

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641SubstrateBy similarity
Binding sitei114 – 1141SubstrateBy similarity
Binding sitei160 – 1601SubstrateBy similarity
Binding sitei167 – 1671SubstrateBy similarity
Binding sitei191 – 1911SubstrateBy similarity
Binding sitei243 – 2431SubstrateBy similarity
Binding sitei261 – 2611Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-2322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolabile glutaminase (EC:3.5.1.2)
Alternative name(s):
Glutaminase A
Gene namesi
Name:glsA
Ordered Locus Names:RHE_CH02311
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Thermolabile glutaminasePRO_0000110619Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi347834.RHE_CH02311.

Structurei

3D structure databases

ProteinModelPortaliO87405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.Curated

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.
OMAiTKQCAIE.
OrthoDBiEOG6N94BK.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

O87405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLQATLDS IYTDILPRIG EGKVADYIPE LAKIDPRQFG MAIVTVDGQV
60 70 80 90 100
FRVGDADIAF SIQSISKVFM LTLALGKVGE GLWKRVGREP SGSAFNSIVQ
110 120 130 140 150
LEHESGIPRN PFINAGAIAV TDVVMAGHAP REAIGELLRF VRYLADDESI
160 170 180 190 200
TIDDKVARSE TQTGYRNVAL ANFMRAYRNL DHPVDHVLGV YFHQCALAMS
210 220 230 240 250
CEQLARAGLF LAARGSNPMT GHSVVSPKRA RRINALMLTC GHYDGSGDFA
260 270 280 290 300
YHVGLPGKSG VGGGIFAVAP GIASIAVWSP GLNKVGNSQL GAVALEMLAA

RTGWSVFGD
Length:309
Mass (Da):32,987
Last modified:May 16, 2006 - v2
Checksum:iCC678FB5E6A048FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941A → T in AAC63991 (PubMed:10095071).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057158 Genomic DNA. Translation: AAC63991.1.
CP000133 Genomic DNA. Translation: ABC91091.1.
RefSeqiWP_011425571.1. NC_007761.1.
YP_469818.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC91091; ABC91091; RHE_CH02311.
GeneIDi3894584.
KEGGiret:RHE_CH02311.
PATRICi23086148. VBIRhiEtl108884_2727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057158 Genomic DNA. Translation: AAC63991.1.
CP000133 Genomic DNA. Translation: ABC91091.1.
RefSeqiWP_011425571.1. NC_007761.1.
YP_469818.1. NC_007761.1.

3D structure databases

ProteinModelPortaliO87405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH02311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC91091; ABC91091; RHE_CH02311.
GeneIDi3894584.
KEGGiret:RHE_CH02311.
PATRICi23086148. VBIRhiEtl108884_2727.

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.
OMAiTKQCAIE.
OrthoDBiEOG6N94BK.

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-2322-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding a thermolabile glutaminase."
    Calderon J., Huerta-Saquero A., Du Pont G., Duran S.
    Biochim. Biophys. Acta 1444:451-456(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.
  3. Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiGLSA_RHIEC
AccessioniPrimary (citable) accession number: O87405
Secondary accession number(s): Q2K7U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 16, 2006
Last modified: March 4, 2015
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.