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Protein

Urease subunit alpha

Gene

ureC

Organism
Synechococcus sp. (strain WH7805)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation1 Publication

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Kineticsi

  1. KM=0.232 mM for urea1 Publication
  1. Vmax=0.0013 mmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.6.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius.1 Publication

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit gamma (ureA), Urease subunit beta (ureB), Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi138Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi219Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi219Nickel 2; via carbamate groupUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Metal bindingi248Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi274Nickel 2; via tele nitrogenUniRule annotation1
Active sitei322Proton donorUniRule annotation1
Metal bindingi362Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
OrganismiSynechococcus sp. (strain WH7805)
Taxonomic identifieri59931 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002341901 – 569Urease subunit alphaAdd BLAST569

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi59931.WH7805_09809.

Structurei

3D structure databases

ProteinModelPortaliO87402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 569UreaseUniRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
PhylomeDBiO87402.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87402-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYRISRQAY AETYGPTTGD RLRLADTELI LEVEKDFTVY GDEVKFGGGK
60 70 80 90 100
VIRDGMGQSQ TPRAGGAVDT VITNALILDW WGIVKADVGL KDGRIVGIGK
110 120 130 140 150
AGNPDTQAGV TIVVGPGTEA IAGEGHILTA GGIDTHIHFI CPQQIETALA
160 170 180 190 200
SGMTTLMGGG TGPATGTNAT TCTPGAFHIG RMLQAAEGLP VNLGFFGKGN
210 220 230 240 250
ASTPEALEEQ VRAGACGLKL HEDWGTTPAT IDACLSVADR MDVQVCIHTD
260 270 280 290 300
TLNEAGFVED TIAAIKGRTI HTFHTEGAGG GHAPDIIKIC GEANVLPSST
310 320 330 340 350
NPTRPYTRNT LEEHLDMLMV CHHLDPRIPE DVAFAESRIR RETIAAEDIL
360 370 380 390 400
HDLGAFSIIA SDSQAMGRVG EVITRTFQTA HKMKVQRGAL PQDSSRNDNH
410 420 430 440 450
RLKRYIAKVT INPALAHGIS SEVGSIETGK LADLVLWKPG FFGIRPEVVI
460 470 480 490 500
KGGSIVWAQM GDANASIPTP GPVHGRPMFG AFGKALAPSC LTFVSEAAMD
510 520 530 540 550
SDIQRHLGLE RTCMAVKDTR SVGKSALKLN SALPKVSVDP QTYEVFADGE
560
LLTCEPAEVL PLAQRYLLL
Length:569
Mass (Da):60,451
Last modified:November 1, 1998 - v1
Checksum:i78C29686D7AAD5A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056189 Genomic DNA. Translation: AAC61502.1.
RefSeqiWP_006042762.1. NZ_CH724168.1.

Genome annotation databases

PATRICi29903583. VBISynSp124776_2620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056189 Genomic DNA. Translation: AAC61502.1.
RefSeqiWP_006042762.1. NZ_CH724168.1.

3D structure databases

ProteinModelPortaliO87402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi59931.WH7805_09809.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICi29903583. VBISynSp124776_2620.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
PhylomeDBiO87402.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_SYNPV
AccessioniPrimary (citable) accession number: O87402
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.