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Protein

Glutamine synthetase

Gene

glnT

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by methionine sulfoximine, ADP and pyrophosphate, but not by various nitrogen-containing metabolites that inhibit other GS enzymes.1 Publication

Kineticsi

  1. KM=13.3 mM for glutamate1 Publication
  2. KM=33 mM for ammonium1 Publication

    pH dependencei

    Optimum pH is 6.8.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi123Magnesium 1By similarity1
    Metal bindingi125Magnesium 2By similarity1
    Metal bindingi180Magnesium 2By similarity1
    Metal bindingi187Magnesium 2By similarity1
    Binding sitei232L-glutamate; via carbonyl oxygenBy similarity1
    Metal bindingi236Magnesium 1; via pros nitrogenBy similarity1
    Binding sitei240ATPBy similarity1
    Binding sitei291L-glutamateBy similarity1
    Binding sitei315ATPBy similarity1
    Binding sitei315L-glutamateBy similarity1
    Binding sitei320ATPBy similarity1
    Metal bindingi328Magnesium 1By similarity1
    Binding sitei330L-glutamateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLigase
    Biological processNitrogen fixation
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase1 Publication (EC:6.3.1.21 Publication)
    Short name:
    GS1 Publication
    Alternative name(s):
    Glutamate--ammonia ligaseCurated
    Glutamine synthetase III1 Publication
    Short name:
    GSIII1 Publication
    Gene namesi
    Name:glnT1 Publication
    Ordered Locus Names:R00090
    ORF Names:SMc02613
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    Proteomesi
    • UP000001976 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001532271 – 435Glutamine synthetaseAdd BLAST435

    Interactioni

    Subunit structurei

    Homooctamer.1 Publication

    Protein-protein interaction databases

    STRINGi266834.SMc02613.

    Structurei

    3D structure databases

    ProteinModelPortaliO87393.
    SMRiO87393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105C5F. Bacteria.
    COG0174. LUCA.
    HOGENOMiHOG000005156.
    KOiK01915.
    OMAiQWERDHT.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiView protein in InterPro
    IPR008147. Gln_synt_b-grasp.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR017536. Glutamine_synthetase_typeIII.
    PfamiView protein in Pfam
    PF00120. Gln-synt_C. 1 hit.
    SMARTiView protein in SMART
    SM01230. Gln-synt_C. 1 hit.
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR03105. gln_synth_III. 1 hit.
    PROSITEiView protein in PROSITE
    PS00181. GLNA_ATP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O87393-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTLDLSTFAR EKGVKYFMIS YTDLFGGQRA KLVPAEAIAD MQKGGAGFAG
    60 70 80 90 100
    FATWFDLTPA HPDLFALPDA SAVIQLPWKK DVAWVAADCI MDDAPVEQAP
    110 120 130 140 150
    RVVLKKLVAE AAQEGLRVKT GVEPEFFLIS PDGSKISDTF DTAEKPCYDQ
    160 170 180 190 200
    QAIMRRYDVI AEICDYMLEL GWKPYQNDHE DANGQFEMNW EYDDALRTAD
    210 220 230 240 250
    KHSFFKFMVK SIAEKHGLRA TFMPKPFKGL TGNGCHCHIS VWDLAGEVNA
    260 270 280 290 300
    FADNKAEFGL SAEGRHFLGG IMKHASALAA VTNPTVNSYK RINAPRTISG
    310 320 330 340 350
    ATWAPNSVTW TGNNRTHMVR VPGPGRFELR LPDGAVNPYL LQAIIIAAGL
    360 370 380 390 400
    SGVRSKADPG RHYDIDMYKD GHKVTDAPKL PLNLLDALRE YNRDEELQEA
    410 420 430
    LGREFSAAYL KLKQGEWNTY CSQFTEWEHQ TTLDV
    Length:435
    Mass (Da):48,481
    Last modified:November 1, 1998 - v1
    Checksum:i90DE8275496E5400
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF055582 Genomic DNA. Translation: AAC62223.1.
    AL591688 Genomic DNA. Translation: CAC41477.1.
    RefSeqiNP_384196.1. NC_003047.1.
    WP_003532529.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC41477; CAC41477; SMc02613.
    GeneIDi1231721.
    KEGGisme:SMc02613.
    PATRICi23629349. VBISinMel96828_1447.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF055582 Genomic DNA. Translation: AAC62223.1.
    AL591688 Genomic DNA. Translation: CAC41477.1.
    RefSeqiNP_384196.1. NC_003047.1.
    WP_003532529.1. NC_003047.1.

    3D structure databases

    ProteinModelPortaliO87393.
    SMRiO87393.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi266834.SMc02613.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAC41477; CAC41477; SMc02613.
    GeneIDi1231721.
    KEGGisme:SMc02613.
    PATRICi23629349. VBISinMel96828_1447.

    Phylogenomic databases

    eggNOGiENOG4105C5F. Bacteria.
    COG0174. LUCA.
    HOGENOMiHOG000005156.
    KOiK01915.
    OMAiQWERDHT.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiView protein in InterPro
    IPR008147. Gln_synt_b-grasp.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR017536. Glutamine_synthetase_typeIII.
    PfamiView protein in Pfam
    PF00120. Gln-synt_C. 1 hit.
    SMARTiView protein in SMART
    SM01230. Gln-synt_C. 1 hit.
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR03105. gln_synth_III. 1 hit.
    PROSITEiView protein in PROSITE
    PS00181. GLNA_ATP. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLNA3_RHIME
    AccessioniPrimary (citable) accession number: O87393
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: March 15, 2017
    This is version 97 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can grow, in the absence of GSI and GSII glutamine synthetases, without a glutamine supplement in minimal medium that contains both ammonium and glutamate. However, given the high glutamate and ammonium substrate Km values, glutamine synthase is probably a secondary activity of GlnT.1 Publication
    Two forms of glutamine synthetase (GSI and GSIII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSIII is a divergent type with very low sequence similarity to the type I and II enzymes.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.