Reviewed, UniProtKB/Swiss-Prot O87388 (SOXB_RHIME)

Names and origin

Protein names

Recommended name:
    Sarcosine oxidase subunit beta
      Short name=Sarcosine oxidase subunit B
    EC=1.5.3.1

Gene names

Name:	soxB
Ordered Locus Names:	R00085
ORF Names:	SMc02608

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate By similarity.
Catalytic activity	Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
Cofactor	Binds 1 FMN covalently By similarity. FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the soxB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sarcosine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Sarcosine oxidase subunit beta		PRO_0000072045
Regions
	Nucleotide binding	33 – 63	31	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O87388-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 86FA18AAA0E9E324	FASTA	416	45,374
	10 20 30 40 50 60 MRYSALSILL NGLRGNRNWA PAWRQPDPKP HYDVIIVGGG GHGLATAYYL AKEFGITNVA 70 80 90 100 110 120 VLEKNYIGSG NVGRNTTIIR SNYLLPGNNP FYELSMKLWE GLEQDFNFNA MVSQRGVLNL 130 140 150 160 170 180 FHSDAQRDAY TRRGNAMRLH GVDAELLYRA AVRKMLPFLD FDNARFPIQG GLLQRRGGTV 190 200 210 220 230 240 RHDAVAWGYA RGADSRGVDI IQNCEVTGIR RENGRVIGVE TSRGFIGCAK LALAAAGNSS 250 260 270 280 290 300 QVAEMAGLRL PIESHVLQAF VSEGLKPFID GVVTFGAGHF YVSQSDKGGL VFGGDIDGYN 310 320 330 340 350 360 SYAQRGNLAT VEHVAEAGKA MIPALSRVRV LRSWGGIMDM SMDGSPIIDR TPIDNLYLNA 370 380 390 400 410 GWCYGGFKAT PASGFCFAHL LARGAPQKTA AAFRLDRFER GFLIDEKGQG AQPNLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Powers E.L., Vuyyuru V., Kahn M.L. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF055582 Genomic DNA. Translation: AAC62218.1. AL591688 Genomic DNA. Translation: CAC41472.1.
RefSeq	NP_384191.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1231716.
GenomeReviews	Gene locus R00085 in contig AL591688_GR.
KEGG	sme:SMc02608.
NMPDR	fig|266834.1.peg.1379.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O87388.
OMA	GGTVRHD.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC02608-MON.
BRENDA	1.5.3.1. 142.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR006278. SoxB. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR01373. soxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SOXB_RHIME

Accession

Primary (citable) accession number: O87388

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)