ID   SOXA_RHIME              Reviewed;         987 AA.
AC   O87386;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   16-JUN-2009, entry version 62.
DE   RecName: Full=Sarcosine oxidase subunit alpha;
DE            Short=Sarcosine oxidase subunit;
DE            EC=1.5.3.1;
GN   Name=soxA; OrderedLocusNames=R00083; ORFNames=SMc02606;
OS   Rhizobium meliloti (Sinorhizobium meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=382;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   MEDLINE=21396507; PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D.,
RA   Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U.,
RA   Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont
RT   Sinorhizobium meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59.
RC   STRAIN=1021;
RA   Powers E.L., Vuyyuru V., Kahn M.L.;
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to
CC       yield glycine, hydrogen peroxide and 5,10-
CC       methylenetetrahydrofolate.
CC   -!- CATALYTIC ACTIVITY: Sarcosine + H(2)O + O(2) = glycine +
CC       formaldehyde + H(2)O(2).
CC   -!- COFACTOR: FAD.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the gcvT family.
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DR   EMBL; AL591688; CAC41470.1; -; Genomic_DNA.
DR   EMBL; AF055582; AAC62216.1; -; Genomic_DNA.
DR   RefSeq; NP_384189.1; -.
DR   GeneID; 1231714; -.
DR   GenomeReviews; AL591688_GR; R00083.
DR   KEGG; sme:SMc02606; -.
DR   NMPDR; fig|266834.1.peg.1377; -.
DR   HOGENOM; O87386; -.
DR   OMA; O87386; PNALMTI.
DR   BioCyc; SMEL266834:SMC02606-MON; -.
DR   BRENDA; 1.5.3.1; 142.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:EC.
DR   GO; GO:0006546; P:glycine catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR004792; HI0933-like.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006277; SoxA.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   Pfam; PF03486; HI0933_like; 1.
DR   PIRSF; PIRSF037980; SoxA; 1.
DR   PRINTS; PR00368; FADPNR.
DR   TIGRFAMs; TIGR01372; soxA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN         1    987       Sarcosine oxidase subunit alpha.
FT                                /FTId=PRO_0000122628.
SQ   SEQUENCE   987 AA;  105803 MW;  FE811D96C8FABB31 CRC64;
     MSSYRLPKRG LVDRNVPLSF TFDGRPMQGL EGDTLASALL ANGRMLVGRS FKYHRPRGIL
     TAGAAEPNAL VTVGRGGRAE PNTRATMQEL YEGLEARSQN RWPSLAFDIG ALNGLLSPFL
     GAGFYYKTFM WPAPLWEKLY EPVIRRAAGL GKASYEADPD AYEKSWAHCD LLVIGAGPTG
     LAAALTAGRA GARVILVDEG SLPGGSLLSD TATIDGKAAA DFARDTSDEL RSMPNVQVLV
     RTTAFGWYDG NVFGAVERVQ KHVREPASHL PVERLWRIVA GKALLATGAE ERPLVFGGND
     RPGVMMAGAM RAYLNRYGVA PGRTPAIFTT NDTGYTLAQE LEAAGVDVVA IVDSRPAAGV
     DYRGKARLVR EAVVCGTKGG KAISAIEVHH GGRTETIAVD ALAMAGGFDP IIHLACHRGG
     KPVWSAEKAA FLAPGSLKGL EVAGGAAATT GLAACLGEGA ARAEAIVREL GLPCPPVAVV
     KVESEEGIRS PAPLWSIPGI KDKAFVDFQN DVHLKDIGLA VREGYSHVEL AKRYTTSGMA
     TDQGKLSNVN AIGLIAKARG VSPAEVGTTT FRPFYTPVSF GALTGAHTGH HFQPVRKSPL
     HDWAKKHGAV FVETGLWYRS SWFPRSGERT WRESVEREVL NVRKNAGLCD VSMLGKIEIT
     GSDAAEFLNR VYCNAFLKLP VGKARYGLML REDGFIYDDG TTSRLEENRF FMTTTTAYAA
     GVMNHLEFCA QVLWPQLDVR LASITDQWAQ MAIAGPKARM ILQKIVDEDI SDAAFPFLAA
     KEVSLFGGAL HGCLFRISFS GELAYELAVP AGYGESIADA LLEAGKDHGI MPYGVETLSV
     LRIEKGHVTH NEINGTIVPA DLGFGKMVSA GKPDFVGKAM LQREGLTAPD RPQLVGVVPL
     DPQQSFRSGS HILAKGAAAT LENDEGYVTS SAYSPHVGST IALALVRNGR NRHGEEVLVW
     SGLHGESTPA RLCNPVFFDP QNERLHV
//