Reviewed, UniProtKB/Swiss-Prot O87386 (SOXA_RHIME)

Names and origin

Protein names

Recommended name:
    Sarcosine oxidase subunit alpha
      Short name=Sarcosine oxidase subunit
    EC=1.5.3.1

Gene names

Name:	soxA
Ordered Locus Names:	R00083
ORF Names:	SMc02606

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	987 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
Catalytic activity	Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
Cofactor	FAD.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro sarcosine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 987	987	Sarcosine oxidase subunit alpha		PRO_0000122628

Sequences

	Sequence		Length	Mass (Da)
	O87386-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: FE811D96C8FABB31	FASTA	987	105,803
	10 20 30 40 50 60 MSSYRLPKRG LVDRNVPLSF TFDGRPMQGL EGDTLASALL ANGRMLVGRS FKYHRPRGIL 70 80 90 100 110 120 TAGAAEPNAL VTVGRGGRAE PNTRATMQEL YEGLEARSQN RWPSLAFDIG ALNGLLSPFL 130 140 150 160 170 180 GAGFYYKTFM WPAPLWEKLY EPVIRRAAGL GKASYEADPD AYEKSWAHCD LLVIGAGPTG 190 200 210 220 230 240 LAAALTAGRA GARVILVDEG SLPGGSLLSD TATIDGKAAA DFARDTSDEL RSMPNVQVLV 250 260 270 280 290 300 RTTAFGWYDG NVFGAVERVQ KHVREPASHL PVERLWRIVA GKALLATGAE ERPLVFGGND 310 320 330 340 350 360 RPGVMMAGAM RAYLNRYGVA PGRTPAIFTT NDTGYTLAQE LEAAGVDVVA IVDSRPAAGV 370 380 390 400 410 420 DYRGKARLVR EAVVCGTKGG KAISAIEVHH GGRTETIAVD ALAMAGGFDP IIHLACHRGG 430 440 450 460 470 480 KPVWSAEKAA FLAPGSLKGL EVAGGAAATT GLAACLGEGA ARAEAIVREL GLPCPPVAVV 490 500 510 520 530 540 KVESEEGIRS PAPLWSIPGI KDKAFVDFQN DVHLKDIGLA VREGYSHVEL AKRYTTSGMA 550 560 570 580 590 600 TDQGKLSNVN AIGLIAKARG VSPAEVGTTT FRPFYTPVSF GALTGAHTGH HFQPVRKSPL 610 620 630 640 650 660 HDWAKKHGAV FVETGLWYRS SWFPRSGERT WRESVEREVL NVRKNAGLCD VSMLGKIEIT 670 680 690 700 710 720 GSDAAEFLNR VYCNAFLKLP VGKARYGLML REDGFIYDDG TTSRLEENRF FMTTTTAYAA 730 740 750 760 770 780 GVMNHLEFCA QVLWPQLDVR LASITDQWAQ MAIAGPKARM ILQKIVDEDI SDAAFPFLAA 790 800 810 820 830 840 KEVSLFGGAL HGCLFRISFS GELAYELAVP AGYGESIADA LLEAGKDHGI MPYGVETLSV 850 860 870 880 890 900 LRIEKGHVTH NEINGTIVPA DLGFGKMVSA GKPDFVGKAM LQREGLTAPD RPQLVGVVPL 910 920 930 940 950 960 DPQQSFRSGS HILAKGAAAT LENDEGYVTS SAYSPHVGST IALALVRNGR NRHGEEVLVW 970 980 SGLHGESTPA RLCNPVFFDP QNERLHV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[2]	Powers E.L., Vuyyuru V., Kahn M.L. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC41470.1. AF055582 Genomic DNA. Translation: AAC62216.1.
RefSeq	NP_384189.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1231714.
GenomeReviews	Gene locus R00083 in contig AL591688_GR.
KEGG	sme:SMc02606.
NMPDR	fig|266834.1.peg.1377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O87386.
OMA	O87386. PNALMTI.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC02606-MON.
BRENDA	1.5.3.1. 142.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR004792. HI0933-like. IPR016040. NAD(P)-bd_dom. IPR006277. SoxA. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. PF03486. HI0933_like. 1 hit. [Graphical view]
PIRSF	PIRSF037980. SoxA. 1 hit.
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01372. soxA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SOXA_RHIME

Accession

Primary (citable) accession number: O87386

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)