ID   O87361_STAAU            Unreviewed;       665 AA.
AC   O87361;
DT   01-NOV-1998, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1998, sequence version 1.
DT   27-MAR-2024, entry version 125.
DE   SubName: Full=MobA/MobL family protein {ECO:0000313|EMBL:NUY12087.1};
DE   SubName: Full=Nicking enzyme {ECO:0000313|EMBL:AAQ17176.1};
DE   SubName: Full=OriT nickase {ECO:0000313|EMBL:AAC61938.1, ECO:0000313|EMBL:ACZ68642.1};
DE   SubName: Full=OriT relaxase Nes {ECO:0000313|EMBL:CAR62405.1};
GN   Name=nes {ECO:0000313|EMBL:AAC61938.1};
GN   ORFNames=BN1321_460007 {ECO:0000313|EMBL:CRI20850.1}, GO782_04285
GN   {ECO:0000313|EMBL:MVH47837.1}, GQX37_05950
GN   {ECO:0000313|EMBL:NUY12087.1}, PGO1_p08 {ECO:0000313|EMBL:CAR62405.1},
GN   SAMEA2078260_02856 {ECO:0000313|EMBL:CAA4403206.1}, SAMEA2081063_02873
GN   {ECO:0000313|EMBL:CAA4710499.1}, SAP014A_018
GN   {ECO:0000313|EMBL:ADA61325.1}, SAP015D_002
GN   {ECO:0000313|EMBL:ADA62799.1}, SAP068A_007
GN   {ECO:0000313|EMBL:ACZ68642.1}, SAP069A_033
GN   {ECO:0000313|EMBL:ACZ68723.1}, SAP079A_020
GN   {ECO:0000313|EMBL:ADA62090.1}, SAP080A_038
GN   {ECO:0000313|EMBL:ADA62161.1}, SAP082A_025
GN   {ECO:0000313|EMBL:ADA62197.1}, VRA0057 {ECO:0000313|EMBL:AAQ17176.1};
OS   Staphylococcus aureus.
OG   Plasmid SAP014A {ECO:0000313|EMBL:ADA61325.1},
OG   Plasmid SAP015B {ECO:0000313|EMBL:ADA62799.1},
OG   Plasmid SAP068A {ECO:0000313|EMBL:ACZ68642.1},
OG   Plasmid SAP069A {ECO:0000313|EMBL:ACZ68723.1},
OG   Plasmid SAP079A {ECO:0000313|EMBL:ADA62090.1},
OG   Plasmid SAP080A {ECO:0000313|EMBL:ADA62161.1},
OG   Plasmid SAP082A {ECO:0000313|EMBL:ADA62197.1},
OG   Plasmid pGO1 {ECO:0000313|EMBL:CAR62405.1},
OG   Plasmid pLW043 {ECO:0000313|EMBL:AAQ17176.1}, and
OG   Plasmid pSK41 {ECO:0000313|EMBL:AAC61938.1}.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280 {ECO:0000313|EMBL:AAC61938.1};
RN   [1] {ECO:0000313|EMBL:AAC61938.1}
RP   NUCLEOTIDE SEQUENCE.
RC   PLASMID=pSK41 {ECO:0000313|EMBL:AAC61938.1};
RX   PubMed=9721269;
RA   Berg T., Firth N., Apisiridej S., Hettiaratchi A., Leelaporn A.,
RA   Skurray R.A.;
RT   "Complete nucleotide sequence of pSK41: evolution of staphylococcal
RT   conjugative multiresistance plasmids.";
RL   J. Bacteriol. 180:4350-4359(1998).
RN   [2] {ECO:0000313|EMBL:AAQ17176.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   PLASMID=pLW043 {ECO:0000313|EMBL:AAQ17176.1};
RX   PubMed=14645850; DOI=10.1126/science.1090956;
RA   Weigel L.M., Clewell D.B., Gill S.R., Clark N.C., McDougal L.K.,
RA   Flannagan S.E., Kolonay J.F., Shetty J., Killgore G.E., Tenover F.C.;
RT   "Genetic analysis of a high-level vancomycin-resistant isolate of
RT   Staphylococcus aureus.";
RL   Science 302:1569-1571(2003).
RN   [3] {ECO:0000313|EMBL:AAQ17176.1}
RP   NUCLEOTIDE SEQUENCE.
RC   PLASMID=pLW043 {ECO:0000313|EMBL:AAQ17176.1};
RA   Gill S., Kolonay J., Shetty J., Tenover F., Weigel L.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:CAR62405.1}
RP   NUCLEOTIDE SEQUENCE.
RC   PLASMID=pGO1 {ECO:0000313|EMBL:CAR62405.1};
RX   PubMed=19298835; DOI=10.1016/j.plasmid.2009.03.001;
RA   Caryl J.A., O'Neill A.J.;
RT   "Complete nucleotide sequence of pGO1, the prototype conjugative plasmid
RT   from the Staphylococci.";
RL   Plasmid 62:35-38(2009).
RN   [5] {ECO:0000313|EMBL:ACZ68642.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CDC58 {ECO:0000313|EMBL:ADA61325.1}, CDC61
RC   {ECO:0000313|EMBL:ADA62799.1}, CDCGA672 {ECO:0000313|EMBL:ADA62090.1},
RC   CDCPANICU {ECO:0000313|EMBL:ADA62197.1}, CDCTN147
RC   {ECO:0000313|EMBL:ADA62161.1}, PM64 {ECO:0000313|EMBL:ACZ68642.1}, and
RC   PM79 {ECO:0000313|EMBL:ACZ68723.1};
RC   PLASMID=SAP014A {ECO:0000313|EMBL:ADA61325.1}, SAP015B
RC   {ECO:0000313|EMBL:ADA62799.1}, SAP068A {ECO:0000313|EMBL:ACZ68642.1},
RC   SAP069A {ECO:0000313|EMBL:ACZ68723.1}, SAP079A
RC   {ECO:0000313|EMBL:ADA62090.1}, SAP080A {ECO:0000313|EMBL:ADA62161.1},
RC   and SAP082A {ECO:0000313|EMBL:ADA62197.1};
RA   Gill J., Borman J., Shetty J., Hostetler J., Durkin S., Montgomery B.;
RL   Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:ACZ68642.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CDC58 {ECO:0000313|EMBL:ADA61325.1}, CDC61
RC   {ECO:0000313|EMBL:ADA62799.1}, PM64 {ECO:0000313|EMBL:ACZ68642.1}, and
RC   PM79 {ECO:0000313|EMBL:ACZ68723.1};
RC   PLASMID=SAP014A {ECO:0000313|EMBL:ADA61325.1}, SAP015B
RC   {ECO:0000313|EMBL:ADA62799.1}, SAP068A {ECO:0000313|EMBL:ACZ68642.1},
RC   and SAP069A {ECO:0000313|EMBL:ACZ68723.1};
RA   Summers A.O., Shearer J., Wireman J.;
RL   Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:ADA62090.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CDCGA672 {ECO:0000313|EMBL:ADA62090.1}, CDCPANICU
RC   {ECO:0000313|EMBL:ADA62197.1}, and CDCTN147
RC   {ECO:0000313|EMBL:ADA62161.1};
RC   PLASMID=SAP079A {ECO:0000313|EMBL:ADA62090.1}, SAP080A
RC   {ECO:0000313|EMBL:ADA62161.1}, and SAP082A
RC   {ECO:0000313|EMBL:ADA62197.1};
RX   PubMed=20585117; DOI=10.1128/AAC.00351-10;
RA   McDougal L.K., Fosheim G.E., Nicholson A., Bulens S.N., Limbago B.M.,
RA   Shearer J.E., Summers A.O., Patel J.B.;
RT   "Emergence of Resistance among USA300 Methicillin-Resistant Staphylococcus
RT   aureus Isolates Causing Invasive Disease in the United States.";
RL   Antimicrob. Agents Chemother. 54:3804-3811(2010).
RN   [8] {ECO:0007829|PDB:4HTE}
RP   X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 254-593 IN COMPLEX WITH CA(2+).
RX   PubMed=23359708; DOI=10.1073/pnas.1219701110;
RA   Edwards J.S., Betts L., Frazier M.L., Pollet R.M., Kwong S.M., Walton W.G.,
RA   Ballentine W.K., Huang J.J., Habibi S., Del Campo M., Meier J.L.,
RA   Dervan P.B., Firth N., Redinbo M.R.;
RT   "Molecular basis of antibiotic multiresistance transfer in Staphylococcus
RT   aureus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:2804-2809(2013).
RN   [9] {ECO:0000313|EMBL:CRI20850.1, ECO:0000313|Proteomes:UP000039437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AH1 {ECO:0000313|EMBL:CRI20850.1,
RC   ECO:0000313|Proteomes:UP000039437};
RA   Syromyatnikov M.Y., Popov V.N.;
RL   Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
RN   [10] {ECO:0000313|EMBL:MVH47837.1, ECO:0000313|Proteomes:UP000442194}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S_3122.LTHB.DN {ECO:0000313|EMBL:MVH47837.1,
RC   ECO:0000313|Proteomes:UP000442194};
RA   Stine O.C.;
RT   "Implementation of targeted gown and glove precautions to prevent
RT   Staphylococcus aureus acquisition in community-based nursing homes.";
RL   Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases.
RN   [11] {ECO:0000313|Proteomes:UP000442696, ECO:0000313|Proteomes:UP000443506}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T012_N16_C08 {ECO:0000313|EMBL:CAA4403206.1,
RC   ECO:0000313|Proteomes:UP000442696}, and T065_N03_C06
RC   {ECO:0000313|EMBL:CAA4710499.1, ECO:0000313|Proteomes:UP000443506};
RG   Pathogen Informatics;
RL   Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
RN   [12] {ECO:0000313|EMBL:NUY12087.1, ECO:0000313|Proteomes:UP000547874}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UE1097 {ECO:0000313|EMBL:NUY12087.1,
RC   ECO:0000313|Proteomes:UP000547874};
RX   PubMed=32562543;
RA   Castro B.E., Berrio M., Vargas M.L., Carvajal L.P., Millan L.V., Rios R.,
RA   Hernandez A.K., Rincon S., Cubides P., Forero E., Dinh A., Seas C.,
RA   Munita J.M., Arias C.A., Reyes J., Diaz L.;
RT   "Detection of heterogeneous vancomycin intermediate resistance in MRSA
RT   isolates from Latin America.";
RL   J. Antimicrob. Chemother. 0:0-0(2020).
CC   -!- SIMILARITY: Belongs to the MobA/MobL family.
CC       {ECO:0000256|ARBA:ARBA00010873}.
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DR   EMBL; AF051917; AAC61938.1; -; Genomic_DNA.
DR   EMBL; AE017171; AAQ17176.1; -; Genomic_DNA.
DR   EMBL; GQ900421; ACZ68642.1; -; Genomic_DNA.
DR   EMBL; GQ900422; ACZ68723.1; -; Genomic_DNA.
DR   EMBL; GQ900379; ADA61325.1; -; Genomic_DNA.
DR   EMBL; GQ900432; ADA62090.1; -; Genomic_DNA.
DR   EMBL; GQ900433; ADA62161.1; -; Genomic_DNA.
DR   EMBL; GQ900434; ADA62197.1; -; Genomic_DNA.
DR   EMBL; GQ900501; ADA62799.1; -; Genomic_DNA.
DR   EMBL; CACTQT010000031; CAA4403206.1; -; Genomic_DNA.
DR   EMBL; CACTWD010000031; CAA4710499.1; -; Genomic_DNA.
DR   EMBL; FM207042; CAR62405.1; -; Genomic_DNA.
DR   EMBL; CVOQ01000041; CRI20850.1; -; Genomic_DNA.
DR   EMBL; WPRT01000100; MVH47837.1; -; Genomic_DNA.
DR   EMBL; JAANEC010000062; NUY12087.1; -; Genomic_DNA.
DR   RefSeq; NP_863609.1; NC_005024.1.
DR   RefSeq; NP_878032.1; NC_005054.1.
DR   RefSeq; WP_001252101.1; NZ_WWCF01000338.1.
DR   RefSeq; YP_002790904.1; NC_012547.1.
DR   RefSeq; YP_006937638.1; NC_013320.1.
DR   RefSeq; YP_006938308.1; NC_013338.1.
DR   RefSeq; YP_006938388.1; NC_013339.1.
DR   RefSeq; YP_006938483.1; NC_013342.1.
DR   RefSeq; YP_006938553.1; NC_013343.1.
DR   RefSeq; YP_006938590.1; NC_013344.1.
DR   PDB; 4HTE; X-ray; 3.00 A; A=254-593.
DR   PDBsum; 4HTE; -.
DR   PATRIC; fig|1280.3497.peg.2884; -.
DR   Proteomes; UP000039437; Unassembled WGS sequence.
DR   Proteomes; UP000442194; Unassembled WGS sequence.
DR   Proteomes; UP000442696; Unassembled WGS sequence.
DR   Proteomes; UP000443506; Unassembled WGS sequence.
DR   Proteomes; UP000547874; Unassembled WGS sequence.
DR   Gene3D; 1.10.167.30; -; 1.
DR   Gene3D; 1.20.58.1730; -; 1.
DR   Gene3D; 1.20.58.1740; -; 1.
DR   Gene3D; 3.30.930.30; -; 1.
DR   InterPro; IPR005053; MobA_MobL.
DR   Pfam; PF03389; MobA_MobL; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4HTE}; Calcium {ECO:0007829|PDB:4HTE};
KW   Conjugation {ECO:0000256|ARBA:ARBA00022971};
KW   Metal-binding {ECO:0007829|PDB:4HTE};
KW   Plasmid {ECO:0000313|EMBL:AAC61938.1}.
FT   DOMAIN          17..216
FT                   /note="MobA/MobL protein"
FT                   /evidence="ECO:0000259|Pfam:PF03389"
FT   REGION          645..665
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         561
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0007829|PDB:4HTE"
FT   BINDING         569
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0007829|PDB:4HTE"
SQ   SEQUENCE   665 AA;  78909 MW;  629A3615725E0D50 CRC64;
     MAMYHFQNKF VSKANGQSAT AKSAYNSASR IKDFKENEFK DYSNKQCDYS EILLPNNADD
     KFKDREYLWN KVHDVENRKN SQVAREIIIG LPNEFDPNSN IELAKEFAES LSNEGMIVDL
     NIHKINEENP HAHLLCTLRG LDKNNEFEPK RKGNDYIRDW NTKEKHNEWR KRWENVQNKH
     LEKNGFSVRV SADSYKNQNI DLEPTKKEGW KARKFEDETG KKSSISKHNE SIKKRNQQKI
     DKMFNEVDDM KSHKLNAFSY MNKSDSTTLK NMAKDLKIYV TPINMYKENE RLYDLKQKTS
     LITDDEDRLN KIEDIEDRQK KLESINEVFE KQAGIFFDKN YPDQSLNYSD DEKIFITRTI
     LNDRDVLPAN NELEDIVKEK RIKEAQISLN TVLGNRDISL ESIEKESNFF ADKLSNILEK
     NNLSFDDVLE NKHEGMEDSL KIDYYTNKLE VFRNAENILE DYYDVQIKEL FTDDEDYKAF
     NEVTDIKEKQ QLIDFKTYHG TENTIEMLET GNFIPKYSDE DRKYITEQVK LLQEKEFKPN
     KNQHDKFVFG AIQKKLLSEY DFDYSDNNDL KHLYQESNEV GDEISKDNIE EFYEGNDILV
     DKETYNNYNR KQQAYGLINA GLDSMIFNFN EIFRERMPKY INHQYKGKNH SKQRHELKNK
     RGMHL
//