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O87331 (RELA_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene names
Name:relA
Synonyms:rel
Ordered Locus Names:Cgl1653, cg1861
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length760 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. It also has (p)ppGpp-degrading activities.

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 760760GTP pyrophosphokinase
PRO_0000166545

Regions

Domain77 – 17498HD
Domain684 – 75875ACT

Experimental info

Sequence conflict2621E → G Ref.1
Sequence conflict2621E → G Ref.3

Sequences

Sequence LengthMass (Da)Tools
O87331 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: 1A72E595DE85E073

FASTA76084,447
        10         20         30         40         50         60 
MSLERNTQKS SMGVRSMSAR LARSLTGNRV RTNPVLDPLL SIHRQFHPRA DVQVLERAYD 

        70         80         90        100        110        120 
TAERLHDGVI RKSGDPYITH PLAVATIAAE IGMDTTTLVA ALLHDTVEDT DYSLDDLTRD 

       130        140        150        160        170        180 
FGEEVARLVD GVTKLDKVAL GAAAEAETIR KMIVAMSQDP RVLVIKVADR LHNMRTMRFL 

       190        200        210        220        230        240 
PPEKQAKKAR QTLEVIAPLA HRLGMASVKW ELEDLSFAIL YPKKYEEIVR LVADRAPSRD 

       250        260        270        280        290        300 
RYLKEIIDQV TGGLRENNIA AEVLGRPKHY WSIYQKMIVR GRDFDDIFDL VGIRILVDNV 

       310        320        330        340        350        360 
NNCYAAIGVV HSLFNALPGR FKDYISAPRF GVYQSLHTTV MGPGGKPLEV QARTHDMHYN 

       370        380        390        400        410        420 
AEFGIAAHWR YKETKGSHSG EQAEVDQMAW MRQLLDWQKE AADPNEFLDS LRYDLTSKQI 

       430        440        450        460        470        480 
FVFTPKGDVV NLPVNSTPVD FAYAVHTEVG HRCIGAKING KLVALETKLK SGDRVEVFTS 

       490        500        510        520        530        540 
KDQNAGPSRG WQEFVVSPRA KAKIRQWFAK ERREEYLEAG RDALAAVIQR GGLPMHRLFT 

       550        560        570        580        590        600 
ASSMKTVATE LHYPDVDALY TAIGSGSVSA QHVVNRLMAI FGDEEDAEDA LVARTPFSEL 

       610        620        630        640        650        660 
VNSRATTESS TGILVEGSPD VMAKLAKCCM PVPGDEIFGF VTRGGGVSVH RTDCTNVEKL 

       670        680        690        700        710        720 
KEEPERIVSV SWASEGQGSV FSATLQLEAL DRAGLLFELT RVINEQKVSV TAMNSHCSED 

       730        740        750        760 
RVATVRFTFA VSDTKQLGSL MTQLRNAEGV FDVYRVTSGG 

« Hide

References

« Hide 'large scale' references
[1]"The role of the Corynebacterium glutamicum rel gene in (p)ppGpp metabolism."
Wehmeier L., Schaefer A., Burkovski A., Kraemer R., Mechold U., Malke H., Puehler A., Kalinowski J.
Microbiology 144:1853-1862(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF038651 Genomic DNA. Translation: AAC35494.1.
BA000036 Genomic DNA. Translation: BAB99046.1.
BX927153 Genomic DNA. Translation: CAF20036.1.
RefSeqNP_600866.1. NC_003450.3.
YP_225937.1. NC_006958.1.

3D structure databases

ProteinModelPortalO87331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg1861.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99046; BAB99046; BAB99046.
CAF20036; CAF20036; cg1861.
GeneID1019621.
3343569.
KEGGcgb:cg1861.
cgl:NCgl1590.
PATRIC21495342. VBICorGlu203724_1614.

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018301.
KOK00951.
K01139.
OMALDWLNFV.
OrthoDBEOG6SV551.
ProtClustDBCLSK811437.

Enzyme and pathway databases

UniPathwayUPA00908; UER00884.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRELA_CORGL
AccessionPrimary (citable) accession number: O87331
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 11, 2002
Last modified: November 13, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways