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Protein

GTP pyrophosphokinase

Gene

relA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. It also has (p)ppGpp-degrading activities.

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathwayi: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GTP pyrophosphokinase (relA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-11238-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
Synonyms:rel
Ordered Locus Names:Cgl1653, cg1861
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001665451 – 760GTP pyrophosphokinaseAdd BLAST760

Interactioni

Protein-protein interaction databases

STRINGi196627.NCgl1590.

Structurei

3D structure databases

ProteinModelPortaliO87331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 174HDAdd BLAST98
Domaini684 – 758ACTPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiENOG4105CWR. Bacteria.
COG0317. LUCA.
HOGENOMiHOG000018301.
KOiK00951.
K01139.
OMAiHGITIHR.

Family and domain databases

CDDicd05399. NT_Rel-Spo_like. 1 hit.
cd01668. TGS_RelA_SpoT. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
IPR033655. TGS_RelA.
[Graphical view]
PfamiPF13291. ACT_4. 1 hit.
PF13328. HD_4. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLERNTQKS SMGVRSMSAR LARSLTGNRV RTNPVLDPLL SIHRQFHPRA
60 70 80 90 100
DVQVLERAYD TAERLHDGVI RKSGDPYITH PLAVATIAAE IGMDTTTLVA
110 120 130 140 150
ALLHDTVEDT DYSLDDLTRD FGEEVARLVD GVTKLDKVAL GAAAEAETIR
160 170 180 190 200
KMIVAMSQDP RVLVIKVADR LHNMRTMRFL PPEKQAKKAR QTLEVIAPLA
210 220 230 240 250
HRLGMASVKW ELEDLSFAIL YPKKYEEIVR LVADRAPSRD RYLKEIIDQV
260 270 280 290 300
TGGLRENNIA AEVLGRPKHY WSIYQKMIVR GRDFDDIFDL VGIRILVDNV
310 320 330 340 350
NNCYAAIGVV HSLFNALPGR FKDYISAPRF GVYQSLHTTV MGPGGKPLEV
360 370 380 390 400
QARTHDMHYN AEFGIAAHWR YKETKGSHSG EQAEVDQMAW MRQLLDWQKE
410 420 430 440 450
AADPNEFLDS LRYDLTSKQI FVFTPKGDVV NLPVNSTPVD FAYAVHTEVG
460 470 480 490 500
HRCIGAKING KLVALETKLK SGDRVEVFTS KDQNAGPSRG WQEFVVSPRA
510 520 530 540 550
KAKIRQWFAK ERREEYLEAG RDALAAVIQR GGLPMHRLFT ASSMKTVATE
560 570 580 590 600
LHYPDVDALY TAIGSGSVSA QHVVNRLMAI FGDEEDAEDA LVARTPFSEL
610 620 630 640 650
VNSRATTESS TGILVEGSPD VMAKLAKCCM PVPGDEIFGF VTRGGGVSVH
660 670 680 690 700
RTDCTNVEKL KEEPERIVSV SWASEGQGSV FSATLQLEAL DRAGLLFELT
710 720 730 740 750
RVINEQKVSV TAMNSHCSED RVATVRFTFA VSDTKQLGSL MTQLRNAEGV
760
FDVYRVTSGG
Length:760
Mass (Da):84,447
Last modified:July 11, 2002 - v2
Checksum:i1A72E595DE85E073
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262E → G (PubMed:9695918).Curated1
Sequence conflicti262E → G (PubMed:12948626).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038651 Genomic DNA. Translation: AAC35494.1.
BA000036 Genomic DNA. Translation: BAB99046.1.
BX927153 Genomic DNA. Translation: CAF20036.1.
RefSeqiNP_600866.1. NC_003450.3.
WP_003855910.1. NC_003450.3.
WP_011265777.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99046; BAB99046; BAB99046.
CAF20036; CAF20036; cg1861.
GeneIDi1019621.
KEGGicgb:cg1861.
cgl:NCgl1590.
PATRICi21495342. VBICorGlu203724_1614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038651 Genomic DNA. Translation: AAC35494.1.
BA000036 Genomic DNA. Translation: BAB99046.1.
BX927153 Genomic DNA. Translation: CAF20036.1.
RefSeqiNP_600866.1. NC_003450.3.
WP_003855910.1. NC_003450.3.
WP_011265777.1. NC_006958.1.

3D structure databases

ProteinModelPortaliO87331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.NCgl1590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99046; BAB99046; BAB99046.
CAF20036; CAF20036; cg1861.
GeneIDi1019621.
KEGGicgb:cg1861.
cgl:NCgl1590.
PATRICi21495342. VBICorGlu203724_1614.

Phylogenomic databases

eggNOGiENOG4105CWR. Bacteria.
COG0317. LUCA.
HOGENOMiHOG000018301.
KOiK00951.
K01139.
OMAiHGITIHR.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciCORYNE:G18NG-11238-MONOMER.

Family and domain databases

CDDicd05399. NT_Rel-Spo_like. 1 hit.
cd01668. TGS_RelA_SpoT. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
IPR033655. TGS_RelA.
[Graphical view]
PfamiPF13291. ACT_4. 1 hit.
PF13328. HD_4. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRELA_CORGL
AccessioniPrimary (citable) accession number: O87331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 11, 2002
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.