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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78Carbamoyl phosphateUniRule annotation1
Binding sitei102Carbamoyl phosphateUniRule annotation1
Binding sitei160OrnithineUniRule annotation1
Binding sitei223OrnithineUniRule annotation1
Binding sitei291Carbamoyl phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciNPUN63737:G1GBR-4578-MONOMER
UniPathwayiUPA00068; UER00112

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:Npun_F4480
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001129741 – 306Ornithine carbamoyltransferaseAdd BLAST306

Interactioni

Protein-protein interaction databases

STRINGi63737.Npun_F4480

Structurei

3D structure databases

ProteinModelPortaliO87319
SMRiO87319
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 54Carbamoyl phosphate bindingUniRule annotation4
Regioni129 – 132Carbamoyl phosphate bindingUniRule annotation4
Regioni227 – 228Ornithine bindingUniRule annotation2
Regioni263 – 264Carbamoyl phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV Bacteria
COG0078 LUCA
HOGENOMiHOG000022686
KOiK00611
OMAiDGNNVCN
OrthoDBiPOG091H0543
PhylomeDBiO87319

Family and domain databases

Gene3Di3.40.50.1370, 3 hits
HAMAPiMF_01109 OTCase, 1 hit
InterProiView protein in InterPro
IPR006132 Asp/Orn_carbamoyltranf_P-bd
IPR006130 Asp/Orn_carbamoylTrfase
IPR036901 Asp/Orn_carbamoylTrfase_sf
IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
IPR002292 Orn/put_carbamltrans
IPR024904 OTCase_ArgI
PfamiView protein in Pfam
PF00185 OTCace, 1 hit
PF02729 OTCace_N, 1 hit
PRINTSiPR00100 AOTCASE
PR00102 OTCASE
SUPFAMiSSF53671 SSF53671, 1 hit
TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
PROSITEiView protein in PROSITE
PS00097 CARBAMOYLTRANSFERASE, 1 hit

Sequencei

Sequence statusi: Complete.

O87319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALLGRDLL SLADISSTEL QELLQLATQL KSQQLKLQCN KVLGLLFSKA
60 70 80 90 100
STRTRVSFTV AMYQLGGQVI DLNPNVTQVS RGEPLQDTAR VLDRYLDILA
110 120 130 140 150
IRTFAQQDLE TFAHYAKIPV INALTDAEHP CQVLADLLTI QESFNTLAGL
160 170 180 190 200
TLTYVGDGNN MANSLMLGCA LVGMNVRIAT PDGYEPDSQI VEQARAIANN
210 220 230 240 250
KTEVLLTHDP ELAAKGSTVL YTDVWASMGQ ETEADNRMPI FQPYQISEQL
260 270 280 290 300
LSLAAPEAIV LHCLPAHRGE EITEAVIEGS QSRVWEQAEN RLHAQKALLA

SILGAE
Length:306
Mass (Da):33,439
Last modified:July 22, 2008 - v2
Checksum:iD6D0F8B1B07B601C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti237R → S in AAC36050 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030524 Genomic DNA Translation: AAC36050.1
CP001037 Genomic DNA Translation: ACC82847.1
RefSeqiWP_012410808.1, NC_010628.1

Genome annotation databases

EnsemblBacteriaiACC82847; ACC82847; Npun_F4480
KEGGinpu:Npun_F4480

Similar proteinsi

Entry informationi

Entry nameiOTC_NOSP7
AccessioniPrimary (citable) accession number: O87319
Secondary accession number(s): B2IVN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: July 22, 2008
Last modified: March 28, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health