O87319 (OTC_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	Npun_F4480

Organism

Nostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]

Taxonomic identifier

63737 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112974
Regions
	Region	51 – 55	5	Carbamoyl phosphate binding By similarity
	Region	262 – 265	4	Ornithine binding By similarity
Sites
	Binding site	102	1	Carbamoyl phosphate By similarity
	Binding site	129	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	142	1	Important for structural integrity By similarity
Experimental info
	Sequence conflict	237	1	R → S in AAC36050. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O87319 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: D6D0F8B1B07B601C	FASTA	306	33,439
	10 20 30 40 50 60 MAALLGRDLL SLADISSTEL QELLQLATQL KSQQLKLQCN KVLGLLFSKA STRTRVSFTV 70 80 90 100 110 120 AMYQLGGQVI DLNPNVTQVS RGEPLQDTAR VLDRYLDILA IRTFAQQDLE TFAHYAKIPV 130 140 150 160 170 180 INALTDAEHP CQVLADLLTI QESFNTLAGL TLTYVGDGNN MANSLMLGCA LVGMNVRIAT 190 200 210 220 230 240 PDGYEPDSQI VEQARAIANN KTEVLLTHDP ELAAKGSTVL YTDVWASMGQ ETEADNRMPI 250 260 270 280 290 300 FQPYQISEQL LSLAAPEAIV LHCLPAHRGE EITEAVIEGS QSRVWEQAEN RLHAQKALLA SILGAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and molecular characterization of a presumptive argF, a structural gene encoding ornithine carbamoyl transferase (OCT), in the cyanobacterium Nostoc PCC 73102." Jansson E., Lindblad P. Physiol. Plantarum 103:347-353(1998) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030524 Genomic DNA. Translation: AAC36050.1. CP001037 Genomic DNA. Translation: ACC82847.1.
RefSeq	YP_001867790.1. NC_010628.1.
3D structure databases
ProteinModelPortal	O87319.
ModBase	Search...
Protein-protein interaction databases
STRING	O87319.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6253832.
GenomeReviews	Gene locus Npun_F4480 in contig CP001037_GR.
KEGG	npu:Npun_F4480.
PATRIC	22762205. VBINosPun48114_5252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	KHLLSIK.
PhylomeDB	O87319.
ProtClustDB	PRK00779.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_NOSP7

Accession

Primary (citable) accession number: O87319
Secondary accession number(s): B2IVN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program