Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Carbamoyl phosphateBy similarity1
Binding sitei78Carbamoyl phosphateBy similarity1
Binding sitei102Carbamoyl phosphateBy similarity1
Binding sitei160OrnithineBy similarity1
Binding sitei223OrnithineBy similarity1
Binding sitei273Carbamoyl phosphateBy similarity1
Binding sitei291Carbamoyl phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:Npun_F4480
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001129741 – 306Ornithine carbamoyltransferaseAdd BLAST306

Interactioni

Protein-protein interaction databases

STRINGi63737.Npun_F4480.

Structurei

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31Important for structural integrityBy similarity1
Sitei142Important for structural integrityBy similarity1

3D structure databases

ProteinModelPortaliO87319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 55Carbamoyl phosphate bindingBy similarity5
Regioni129 – 132Carbamoyl phosphate bindingBy similarity4
Regioni227 – 228Ornithine bindingBy similarity2
Regioni262 – 265Carbamoyl phosphate bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiPOG091H0543.
PhylomeDBiO87319.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALLGRDLL SLADISSTEL QELLQLATQL KSQQLKLQCN KVLGLLFSKA
60 70 80 90 100
STRTRVSFTV AMYQLGGQVI DLNPNVTQVS RGEPLQDTAR VLDRYLDILA
110 120 130 140 150
IRTFAQQDLE TFAHYAKIPV INALTDAEHP CQVLADLLTI QESFNTLAGL
160 170 180 190 200
TLTYVGDGNN MANSLMLGCA LVGMNVRIAT PDGYEPDSQI VEQARAIANN
210 220 230 240 250
KTEVLLTHDP ELAAKGSTVL YTDVWASMGQ ETEADNRMPI FQPYQISEQL
260 270 280 290 300
LSLAAPEAIV LHCLPAHRGE EITEAVIEGS QSRVWEQAEN RLHAQKALLA

SILGAE
Length:306
Mass (Da):33,439
Last modified:July 22, 2008 - v2
Checksum:iD6D0F8B1B07B601C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti237R → S in AAC36050 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030524 Genomic DNA. Translation: AAC36050.1.
CP001037 Genomic DNA. Translation: ACC82847.1.
RefSeqiWP_012410808.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC82847; ACC82847; Npun_F4480.
KEGGinpu:Npun_F4480.
PATRICi22762205. VBINosPun48114_5252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030524 Genomic DNA. Translation: AAC36050.1.
CP001037 Genomic DNA. Translation: ACC82847.1.
RefSeqiWP_012410808.1. NC_010628.1.

3D structure databases

ProteinModelPortaliO87319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi63737.Npun_F4480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC82847; ACC82847; Npun_F4480.
KEGGinpu:Npun_F4480.
PATRICi22762205. VBINosPun48114_5252.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiPOG091H0543.
PhylomeDBiO87319.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_NOSP7
AccessioniPrimary (citable) accession number: O87319
Secondary accession number(s): B2IVN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: July 22, 2008
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.