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Protein

Homocitrate synthase

Gene

lys20

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase (lys20)
  2. no protein annotated in this organism
  3. Homoaconitase large subunit (hacA), Homoaconitase small subunit (hacB)
  4. Homoisocitrate dehydrogenase (hicd)
  5. 2-aminoadipate transaminase (lysN)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6722.
TTHE262724:GCAT-1572-MONOMER.
SABIO-RKO87198.
UniPathwayiUPA00033; UER00028.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase (EC:2.3.3.14)
Gene namesi
Name:lys20
Ordered Locus Names:TT_C1550
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000592 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Homocitrate synthasePRO_0000140455Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi262724.TTC1550.

Structurei

Secondary structure

1
376
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117Combined sources
Helixi13 – 164Combined sources
Helixi24 – 3714Combined sources
Beta strandi40 – 445Combined sources
Helixi47 – 493Combined sources
Helixi51 – 6111Combined sources
Beta strandi66 – 7510Combined sources
Helixi77 – 859Combined sources
Beta strandi89 – 957Combined sources
Helixi109 – 12618Combined sources
Beta strandi130 – 1367Combined sources
Turni137 – 1415Combined sources
Helixi144 – 15411Combined sources
Helixi155 – 1573Combined sources
Beta strandi159 – 1657Combined sources
Helixi172 – 18615Combined sources
Turni187 – 1893Combined sources
Beta strandi190 – 1978Combined sources
Helixi203 – 21210Combined sources
Beta strandi217 – 2215Combined sources
Helixi222 – 2243Combined sources
Helixi234 – 24411Combined sources
Helixi246 – 2527Combined sources
Helixi255 – 2573Combined sources
Helixi258 – 26912Combined sources
Turni278 – 2803Combined sources
Turni282 – 2854Combined sources
Helixi290 – 2989Combined sources
Helixi300 – 3034Combined sources
Helixi308 – 3114Combined sources
Helixi342 – 35716Combined sources
Helixi363 – 37412Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZTJX-ray1.80A1-376[»]
2ZTKX-ray1.96A1-376[»]
2ZYFX-ray2.15A1-376[»]
3A9IX-ray1.80A1-376[»]
ProteinModelPortaliO87198.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO87198.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108JZA. Bacteria.
COG0119. LUCA.
KOiK01655.
OMAiKAIQHRL.
OrthoDBiEOG6V1M1Z.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87198-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREWKIIDST LREGEQFEKA NFSTQDKVEI AKALDEFGIE YIEVTTPVAS
60 70 80 90 100
PQSRKDAEVL ASLGLKAKVV THIQCRLDAA KVAVETGVQG IDLLFGTSKY
110 120 130 140 150
LRAAHGRDIP RIIEEAKEVI AYIREAAPHV EVRFSAEDTF RSEEQDLLAV
160 170 180 190 200
YEAVAPYVDR VGLADTVGVA TPRQVYALVR EVRRVVGPRV DIEFHGHNDT
210 220 230 240 250
GCAIANAYEA IEAGATHVDT TILGIGERNG ITPLGGFLAR MYTLQPEYVR
260 270 280 290 300
RKYKLEMLPE LDRMVARMVG VEIPFNNYIT GETAFSHKAG MHLKAIYINP
310 320 330 340 350
EAYEPYPPEV FGVKRKLIIA SRLTGRHAIK ARAEELGLHY GEEELHRVTQ
360 370
HIKALADRGQ LTLEELDRIL REWITA
Length:376
Mass (Da):42,159
Last modified:May 24, 2004 - v2
Checksum:i1B8EEA63C82FCF06
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041A → P in BAA33785 (PubMed:9868782).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018379 Genomic DNA. Translation: BAA33785.1.
AE017221 Genomic DNA. Translation: AAS81892.1.
PIRiT51170.
RefSeqiWP_011173924.1. NC_005835.1.

Genome annotation databases

EnsemblBacteriaiAAS81892; AAS81892; TT_C1550.
KEGGitth:TTC1550.
PATRICi23953527. VBITheThe54392_1545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018379 Genomic DNA. Translation: BAA33785.1.
AE017221 Genomic DNA. Translation: AAS81892.1.
PIRiT51170.
RefSeqiWP_011173924.1. NC_005835.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZTJX-ray1.80A1-376[»]
2ZTKX-ray1.96A1-376[»]
2ZYFX-ray2.15A1-376[»]
3A9IX-ray1.80A1-376[»]
ProteinModelPortaliO87198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262724.TTC1550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS81892; AAS81892; TT_C1550.
KEGGitth:TTC1550.
PATRICi23953527. VBITheThe54392_1545.

Phylogenomic databases

eggNOGiENOG4108JZA. Bacteria.
COG0119. LUCA.
KOiK01655.
OMAiKAIQHRL.
OrthoDBiEOG6V1M1Z.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00028.
BioCyciMetaCyc:MONOMER-6722.
TTHE262724:GCAT-1572-MONOMER.
SABIO-RKO87198.

Miscellaneous databases

EvolutionaryTraceiO87198.
PROiO87198.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Lysine is synthesized through the alpha-aminoadipate pathway in Thermus thermophilus."
    Kosuge T., Hoshino T.
    FEMS Microbiol. Lett. 169:361-367(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB27 / ATCC BAA-163 / DSM 7039.

Entry informationi

Entry nameiHOSC_THET2
AccessioniPrimary (citable) accession number: O87198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 24, 2004
Last modified: June 8, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.