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Protein

Homocitrate synthase

Gene

lys20

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase (lys20)
  2. no protein annotated in this organism
  3. Homoaconitase large subunit (hacA), Homoaconitase small subunit (hacB)
  4. Homoisocitrate dehydrogenase (hicd)
  5. 2-aminoadipate transaminase (lysN)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6722.
SABIO-RKO87198.
UniPathwayiUPA00033; UER00028.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase (EC:2.3.3.14)
Gene namesi
Name:lys20
Ordered Locus Names:TT_C1550
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000592 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404551 – 376Homocitrate synthaseAdd BLAST376

Interactioni

Protein-protein interaction databases

STRINGi262724.TTC1550.

Structurei

Secondary structure

1376
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 11Combined sources7
Helixi13 – 16Combined sources4
Helixi24 – 37Combined sources14
Beta strandi40 – 44Combined sources5
Helixi47 – 49Combined sources3
Helixi51 – 61Combined sources11
Beta strandi66 – 75Combined sources10
Helixi77 – 85Combined sources9
Beta strandi89 – 95Combined sources7
Helixi109 – 126Combined sources18
Beta strandi130 – 136Combined sources7
Turni137 – 141Combined sources5
Helixi144 – 154Combined sources11
Helixi155 – 157Combined sources3
Beta strandi159 – 165Combined sources7
Helixi172 – 186Combined sources15
Turni187 – 189Combined sources3
Beta strandi190 – 197Combined sources8
Helixi203 – 212Combined sources10
Beta strandi217 – 221Combined sources5
Helixi222 – 224Combined sources3
Helixi234 – 244Combined sources11
Helixi246 – 252Combined sources7
Helixi255 – 257Combined sources3
Helixi258 – 269Combined sources12
Turni278 – 280Combined sources3
Turni282 – 285Combined sources4
Helixi290 – 298Combined sources9
Helixi300 – 303Combined sources4
Helixi308 – 311Combined sources4
Helixi342 – 357Combined sources16
Helixi363 – 374Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZTJX-ray1.80A1-376[»]
2ZTKX-ray1.96A1-376[»]
2ZYFX-ray2.15A1-376[»]
3A9IX-ray1.80A1-376[»]
ProteinModelPortaliO87198.
SMRiO87198.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO87198.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 259Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST256

Sequence similaritiesi

Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JZA. Bacteria.
COG0119. LUCA.
KOiK01655.
OMAiRDYVIDK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O87198-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREWKIIDST LREGEQFEKA NFSTQDKVEI AKALDEFGIE YIEVTTPVAS
60 70 80 90 100
PQSRKDAEVL ASLGLKAKVV THIQCRLDAA KVAVETGVQG IDLLFGTSKY
110 120 130 140 150
LRAAHGRDIP RIIEEAKEVI AYIREAAPHV EVRFSAEDTF RSEEQDLLAV
160 170 180 190 200
YEAVAPYVDR VGLADTVGVA TPRQVYALVR EVRRVVGPRV DIEFHGHNDT
210 220 230 240 250
GCAIANAYEA IEAGATHVDT TILGIGERNG ITPLGGFLAR MYTLQPEYVR
260 270 280 290 300
RKYKLEMLPE LDRMVARMVG VEIPFNNYIT GETAFSHKAG MHLKAIYINP
310 320 330 340 350
EAYEPYPPEV FGVKRKLIIA SRLTGRHAIK ARAEELGLHY GEEELHRVTQ
360 370
HIKALADRGQ LTLEELDRIL REWITA
Length:376
Mass (Da):42,159
Last modified:May 24, 2004 - v2
Checksum:i1B8EEA63C82FCF06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104A → P in BAA33785 (PubMed:9868782).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018379 Genomic DNA. Translation: BAA33785.1.
AE017221 Genomic DNA. Translation: AAS81892.1.
PIRiT51170.
RefSeqiWP_011173924.1. NC_005835.1.

Genome annotation databases

EnsemblBacteriaiAAS81892; AAS81892; TT_C1550.
KEGGitth:TT_C1550.
PATRICi23953527. VBITheThe54392_1545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018379 Genomic DNA. Translation: BAA33785.1.
AE017221 Genomic DNA. Translation: AAS81892.1.
PIRiT51170.
RefSeqiWP_011173924.1. NC_005835.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZTJX-ray1.80A1-376[»]
2ZTKX-ray1.96A1-376[»]
2ZYFX-ray2.15A1-376[»]
3A9IX-ray1.80A1-376[»]
ProteinModelPortaliO87198.
SMRiO87198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262724.TTC1550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS81892; AAS81892; TT_C1550.
KEGGitth:TT_C1550.
PATRICi23953527. VBITheThe54392_1545.

Phylogenomic databases

eggNOGiENOG4108JZA. Bacteria.
COG0119. LUCA.
KOiK01655.
OMAiRDYVIDK.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00028.
BioCyciMetaCyc:MONOMER-6722.
SABIO-RKO87198.

Miscellaneous databases

EvolutionaryTraceiO87198.
PROiO87198.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOSC_THET2
AccessioniPrimary (citable) accession number: O87198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 24, 2004
Last modified: November 2, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.