O87170 (O87170_PSEPA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
2-pyrone-4,6-dicarbaxylate hydrolase EMBL BAB88736.1

Submitted name:
2-pyrone-4,6-dicarboxylic acid hydrolase EMBL BAA33799.1

Gene names

Name:

ligI EMBL BAA33799.1

Organism

Pseudomonas paucimobilis (Sphingomonas paucimobilis) EMBL BAA33799.1

Taxonomic identifier

13689 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Hydrolase EMBL BAA33799.1
Technical term	3D-structure PDB 4DIA PDB 4DI8 PDB 4DI9 PDB 4D8L
Gene Ontology (GO)
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O87170 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 95DE7EB8449AADD5	FASTA	293	32,775
	10 20 30 40 50 60 MTNDERILSW NETPSKPRYT PPPGAIDAHC HVFGPMAQFP FSPKAKYLPR DAGPDMLFAL 70 80 90 100 110 120 RDHLGFARNV IVQASCHGTD NAATLDAIAR AQGKARGIAV VDPAIDEAEL AALHEGGMRG 130 140 150 160 170 180 IRFNFLKRLV DDAPKDKFLE VAGRLPAGWH VVIYFEADIL EELRPFMDAI PVPIVIDHMG 190 200 210 220 230 240 RPDVRQGPDG ADMKAFRRLL DSREDIWFKA TCPDRLDPAG PPWDDFARSV APLVADYADR 250 260 270 280 290 VIWGTDWPHP NMQDAIPDDG LVVDMIPRIA PTPELQHKML VTNPMRLYWS EEM

References

[1]	"Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis." Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M. J. Bacteriol. 172:2704-2709(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Genetic and biochemical characterization of a 2-Pyrone-4,6-dicarboxylic acid hydrolase involved in the protocatechuate 4,5-cleavage pathway of Sphingomonas paucimobilis SYK-6." Masai E., Shinohara S., Hara H., Nishikawa S., Katayama Y., Fukuda M. J. Bacteriol. 180:55-62(1999) Cited for: NUCLEOTIDE SEQUENCE. Strain: SYK-6 EMBL BAA33799.1.
[3]	"Genetic and biochemical characterization of a 2-pyrone-4, 6-dicarboxylic acid hydrolase involved in the protocatechuate 4, 5-cleavage pathway of Sphingomonas paucimobilis SYK-6." Masai E., Shinohara S., Hara H., Nishikawa S., Katayama Y., Fukuda M. J. Bacteriol. 181:55-62(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6." Masai E., Momose K., Hara H., Nishikawa S., Katayama Y., Fukuda M. J. Bacteriol. 182:6651-6658(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6." Hara H., Masai E., Katayama Y., Fukuda M. J. Bacteriol. 182:6950-6957(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[6]	"Characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6." Hara H., Masai E., Miyauchi K., Katayama Y., Fukuda M. J. Bacteriol. 185:41-50(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[7]	"Structure and Catalytic Mechanism of LigI: Insight into the Amidohydrolase Enzymes of cog3618 and Lignin Degradation." Hobbs M.E., Malashkevich V., Williams H.J., Xu C., Sauder J.M., Burley S.K., Almo S.C., Raushel F.M. Biochemistry 51:3497-3507(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 2-293.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB015964 Genomic DNA. Translation: BAA33799.1.
AB073227 Genomic DNA. Translation: BAB88736.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4D8L	X-ray	2.00	A	2-293	[»]
4DI8	X-ray	1.81	A/B	2-293	[»]
4DI9	X-ray	1.35	A	2-293	[»]
4DIA	X-ray	2.00	A	2-293	[»]

SMR

O87170. Positions 1-293.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-3467.

Family and domain databases

InterPro

IPR006992. Amidohydro_2.
[Graphical view]

Pfam

PF04909. Amidohydro_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O87170.

Entry information

Entry name

O87170_PSEPA

Accession

Primary (citable) accession number: O87170

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)